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Melgo
Semperviva Fanatic



Registered: 02/11/16
Posts: 698
Loc: Western Europe
Last seen: 2 hours, 21 minutes
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: Zigzagzin] 6
#27931771 - 09/03/22 07:17 AM (1 year, 5 months ago) |
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Since I'm now focusing exclusively on this active mushroom until I figure it out, I've been thinking about it a lot.
For all of us having issues with contamination, I think the first and most simple test is:
- Get a jar fully colonized with clean agar.
- Case it with pasteurized cactus soil (or coir - but haven't tried).
- Put it in fruiting conditions.
- Wait.
I've had 80% successes with this approach. Tiny harvests, but clone-able mushrooms. Very simple, not too much resources & time spent.
Here is an example (that I already posted before, but I replicated multiple times since):

Max I got was 2 flushes, but most time only 1. Each time I see my grain contaminating. Which makes me think something is off with my prep.
I think I got the idea from smalltalk_canceled, so hat's off to him.
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SirPsycho
Purple Belt in Google-Fu



Registered: 01/01/20
Posts: 6,948
Loc: Rent free in your head
Last seen: 2 days, 4 hours
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: Konbri]
#27931780 - 09/03/22 07:26 AM (1 year, 5 months ago) |
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Quote:
Konbri said: Btw, I tried to mimic your grows, SirPsycho, but with no luck. I used tall PP5 bottles, stuffed to the rim. I used pure coco and millet spawn, and the one I cased with humus has triched, the other one I cased with coco is running for almost two months, it was cold shocked, dunked, dryed and rehydrated, the only thing I didn't try is scratching. But I don't give up, I'll try making a bag with only hay + millet spawn and see what it brings.
Did you do a regular S2B type thing with colonized spawn dumped into sub or did you follow Muda's write up?
--------------------
Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖
    "Mist your balls and fan your asshole" - Pandaskis, 2023
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the_chosen_one
On the Darkslide


Registered: 09/11/06
Posts: 2,937
Loc: 1984
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: Melgo]
#27931786 - 09/03/22 07:31 AM (1 year, 5 months ago) |
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Good philosophy for all exotics.
-------------------- "Luck favors the observant." - Workman
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Zigzagzin



Registered: 04/23/21
Posts: 200
Loc: Down South
Last seen: 8 days, 2 hours
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: Rotnpins] 1
#27931799 - 09/03/22 07:46 AM (1 year, 5 months ago) |
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Quote:
Rotnpins said:
Quote:
Zigzagzin said: All this trich is making me nervous.
Me too! I've got some Semperviva ready to go to grain in a day or two... starting to lose the tiny bit of confidence that I had 
I am exactly in the same position. I might try what melgo just mentioned for a couple of my jars to be on the safe side.
-------------------- Namo Tassa Bhagavato Arahato Samma Sambuddhasa
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Rotnpins
🤮 Rotten-Pins 🍄



Registered: 01/11/22
Posts: 4,738
Loc: in (front of) the hood
Last seen: 1 year, 1 month
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: Zigzagzin] 1
#27931801 - 09/03/22 07:47 AM (1 year, 5 months ago) |
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Quote:
Zigzagzin said:
Quote:
Rotnpins said:
Quote:
Zigzagzin said: All this trich is making me nervous.
Me too! I've got some Semperviva ready to go to grain in a day or two... starting to lose the tiny bit of confidence that I had 
I am exactly in the same position. I might try what melgo just mentioned for a couple of my jars to be on the safe side.
For sure, I was thinking the same thing
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Zigzagzin



Registered: 04/23/21
Posts: 200
Loc: Down South
Last seen: 8 days, 2 hours
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: Melgo]
#27931859 - 09/03/22 08:32 AM (1 year, 5 months ago) |
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Is there a specific tek for pasturizing cactus soil or do you use the bucket tek like you would for coir?
-------------------- Namo Tassa Bhagavato Arahato Samma Sambuddhasa
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nosf3r4tu

Registered: 03/26/19
Posts: 775
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: Zigzagzin] 2
#27931925 - 09/03/22 09:02 AM (1 year, 5 months ago) |
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I put my casing in jars and cook it in the oven at 70C for 2-3h.
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YoshiTrainer
Onion tied to belt



Registered: 04/30/22
Posts: 1,244
Loc: Castles made of sand
Last seen: 1 hour, 49 minutes
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: nosf3r4tu] 7
#27931964 - 09/03/22 09:32 AM (1 year, 5 months ago) |
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4th flush starting to get a little Dr. Seuss going on. The tub went outside, hopefully Mom takes over from here.
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Zigzagzin



Registered: 04/23/21
Posts: 200
Loc: Down South
Last seen: 8 days, 2 hours
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: nosf3r4tu]
#27931967 - 09/03/22 09:33 AM (1 year, 5 months ago) |
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Would 76.6C work? My stove doesn't go that low.
-------------------- Namo Tassa Bhagavato Arahato Samma Sambuddhasa
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nosf3r4tu

Registered: 03/26/19
Posts: 775
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: Zigzagzin] 2
#27932012 - 09/03/22 10:06 AM (1 year, 5 months ago) |
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Yeah. But you can also use verm strait out of the bag. And keep it moist with a syringe.
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nosf3r4tu

Registered: 03/26/19
Posts: 775
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: nosf3r4tu]
#27932015 - 09/03/22 10:09 AM (1 year, 5 months ago) |
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People who have problems with the sub and don't see pins in 2 weeks, what are your fruiting temperatures?
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Zigzagzin



Registered: 04/23/21
Posts: 200
Loc: Down South
Last seen: 8 days, 2 hours
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: nosf3r4tu]
#27932018 - 09/03/22 10:10 AM (1 year, 5 months ago) |
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Thanks!
-------------------- Namo Tassa Bhagavato Arahato Samma Sambuddhasa
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Konbri


Registered: 10/16/17
Posts: 141
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: SirPsycho]
#27932021 - 09/03/22 10:11 AM (1 year, 5 months ago) |
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Quote:
SirPsycho said: Did you do a regular S2B type thing with colonized spawn dumped into sub or did you follow Muda's write up?
Yes, it was a regular S2B. Now I'll try Muda's tek (I figured same technique and tried it with pans with no luck, so I concentrated on my nats grow), I'll try pasteurized straw in a bag and I'll try fruiting from spawn jar cased with verm (or cactus soil?)
-------------------- Radio Gnome Invisible
   
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Konbri


Registered: 10/16/17
Posts: 141
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: nosf3r4tu]
#27932025 - 09/03/22 10:14 AM (1 year, 5 months ago) |
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Quote:
nosf3r4tu said: People who have problems with the sub and don't see pins in 2 weeks, what are your fruiting temperatures?
It was 28-29C for the whole month, sometimes dropping to 26-27, as I couldn't make it colder with this crazy hot August. Now it's around 26. I know that temps closer to 20 are much better, but the choise is either 15 or 26
-------------------- Radio Gnome Invisible
   
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Rotnpins
🤮 Rotten-Pins 🍄



Registered: 01/11/22
Posts: 4,738
Loc: in (front of) the hood
Last seen: 1 year, 1 month
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: nosf3r4tu] 1
#27932062 - 09/03/22 10:35 AM (1 year, 5 months ago) |
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T2 Semperviva plates.. going take a transfer and send the rest later today.
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nosf3r4tu

Registered: 03/26/19
Posts: 775
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: Konbri]
#27932108 - 09/03/22 11:03 AM (1 year, 5 months ago) |
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That might be the issue. Ideal temps are 20-23 C
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The Tao
Look at the flowers.


Registered: 09/12/19
Posts: 1,568
Loc: Canada
Last seen: 4 minutes, 20 seconds
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: Rotnpins]
#27932131 - 09/03/22 11:25 AM (1 year, 5 months ago) |
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Nice plates. What are you sending it to?
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stagger



Registered: 05/05/19
Posts: 1,369
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: The Tao]
#27932191 - 09/03/22 12:10 PM (1 year, 5 months ago) |
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lost all my boxes to trich, 1 a few days after casing. Still have grains colonizing that aren't showing any signs of sabotage but I'm not sure I can trust them either. Guess I'll start new plates
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Rotnpins
🤮 Rotten-Pins 🍄



Registered: 01/11/22
Posts: 4,738
Loc: in (front of) the hood
Last seen: 1 year, 1 month
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: The Tao]
#27932234 - 09/03/22 12:47 PM (1 year, 5 months ago) |
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Quote:
The Tao said: Nice plates. What are you sending it to?
50/50 mix of oats & millet and LC for some cakes
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Mycoplex
Sporocarp



Registered: 10/09/21
Posts: 836
Last seen: 12 hours, 8 minutes
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: Melgo] 2
#27932256 - 09/03/22 01:09 PM (1 year, 5 months ago) |
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Quote:
Melgo said: Since I'm now focusing exclusively on this active mushroom until I figure it out, I've been thinking about it a lot.
For all of us having issues with contamination, I think the first and most simple test is:
- Get a jar fully colonized with clean agar.
- Case it with pasteurized cactus soil (or coir - but haven't tried).
- Put it in fruiting conditions.
- Wait.
I've had 80% successes with this approach. Tiny harvests, but clone-able mushrooms. Very simple, not too much resources & time spent.
Here is an example (that I already posted before, but I replicated multiple times since):

Max I got was 2 flushes, but most time only 1. Each time I see my grain contaminating. Which makes me think something is off with my prep.
I think I got the idea from smalltalk_canceled, so hat's off to him.
I think I'll adopt this approach to try narrowing in on my semp contamination. I like the idea of using the jar as a way to cut out the root cause of contam 50/50: If you see the contents of the jar contaminating while fruiting it, you know that the source of contam is not related to spawning to a tub, etc. but rather something with the culture isolation or grain prep.
In my case, I believe the root problem for me will come out to me culture isolation but I'm going to use this strategy and find out.
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