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InvisibleGuerrilla
Bumbaclart


Registered: 01/30/21
Posts: 3,171
Loc: United Kingdom Flag
Re: Post Your New Tools Of The Trade. [Re: Hindsight] * 1
    #27916912 - 08/24/22 04:10 PM (1 year, 8 months ago)

Yea these are just spores on a slide.

I couldn't get a good view of an agar wedge I cut and put on a slide either but I did manage to get a good look at hyphae on an agar plate.

Also looking at a thin slice of some fresh mushroom gills was cool. Could see spores in there besides the actual tissue up close. Only issue with the gills is that some parts were in focus and some not, but I looked into it and this is normal, there's special techniques for getting it just right, or taking several snapshots of each part in focus.

Haven't had a proper chance to play with it yet so any improvements and I'll let you know! :smile:


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Being pissed on does not make you a real man.

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OfflineHindsight
Mad Scientist
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Registered: 01/24/21
Posts: 2,706
Last seen: 1 year, 28 days
Trusted Cultivator
Re: Post Your New Tools Of The Trade. [Re: Guerrilla] * 2
    #27917315 - 08/24/22 09:12 PM (1 year, 8 months ago)

Yeah please do keep me posted. I have seen one or two people post photos of mycelium coming out of spores early on in the germination process, which I don't think I could capture on my scope. I noticed the same thing as you in terms of areas being out of focus when looking at certain things and it makes sense because the focal window is SO narrow that you can really only see a razor-thin plane at any single moment. I have found that this makes looking at mycelium very challenging because we think of mycelium as spreading out on a flat plane but it's really more like if you took a bowl of spaghetti and used a laser to slice through it, then looked at the sliced part. There are things running in every which direction!

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OfflinemmmushTek
Stranger
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Registered: 08/23/22
Posts: 8
Last seen: 1 year, 7 months
Re: Post Your New Tools Of The Trade. [Re: Hindsight]
    #27917429 - 08/24/22 11:13 PM (1 year, 8 months ago)

I got a $10 roasting pan off of Craigslist. Going to try some oven pasteurization. It smells like a sauna, not too shabby

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InvisibleHobbit GDF
Deadhead
Male


Registered: 02/14/19
Posts: 3,489
Loc: Terrapin station
Re: Post Your New Tools Of The Trade. [Re: mmmushTek] * 1
    #27919538 - 08/26/22 10:04 AM (1 year, 8 months ago)



Got a case of 500 plates today. Yay.
Have an order for 160 agar plates, so I got some work to do.
Fun fun

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OnlineHILLBILLY OUTLAWS
Above And Beyond!
Male User Gallery


Registered: 04/21/22
Posts: 3,286
Loc: Luckenbach Texas
Last seen: 47 seconds
Re: Post Your New Tools Of The Trade. [Re: Hobbit GDF]
    #27919591 - 08/26/22 10:46 AM (1 year, 8 months ago)

Quote:

Hobbit GDF said:


Got a case of 500 plates today. Yay.
Have an order for 160 agar plates, so I got some work to do.
Fun fun




Did you have many damaged. I got a 500 pack from Amazon and at least 1/4 of the sleeves were damaged.


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🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
TEAM SPREAD THE LOVE!
Smellyhobbit said:
Embarrassment and bashfulness are leeches on your ability to learn.

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InvisibleGuerrilla
Bumbaclart


Registered: 01/30/21
Posts: 3,171
Loc: United Kingdom Flag
Re: Post Your New Tools Of The Trade. [Re: HILLBILLY OUTLAW]
    #27919594 - 08/26/22 10:47 AM (1 year, 8 months ago)

Quote:

HILLBILLY OUTLAW said:
Quote:

Hobbit GDF said:


Got a case of 500 plates today. Yay.
Have an order for 160 agar plates, so I got some work to do.
Fun fun




Did you have many damaged. I got a 500 pack from Amazon and at least 1/4 of the sleeves were damaged.




This happens to me with cheaper petris. Sometimes it's worth it cause of the price and usually it's only 1 dish in the sleeves that are damaged. Or I could pay 3x the price for better quality.


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Being pissed on does not make you a real man.

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Offlinerumfor69
Bodhicitta Cultivator
Male


Registered: 08/05/11
Posts: 7,131
Loc: In the Gills
Last seen: 3 hours, 29 minutes
Re: Post Your New Tools Of The Trade. [Re: HILLBILLY OUTLAW]
    #27919597 - 08/26/22 10:48 AM (1 year, 8 months ago)

Quote:

HILLBILLY OUTLAW said:
Quote:

Hobbit GDF said:


Got a case of 500 plates today. Yay.
Have an order for 160 agar plates, so I got some work to do.
Fun fun




Did you have many damaged. I got a 500 pack from Amazon and at least 1/4 of the sleeves were damaged.




I'd be grumpy about that and seeking some refund


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Links For Beginners And Beyond:mspoil:

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InvisibleGuerrilla
Bumbaclart


Registered: 01/30/21
Posts: 3,171
Loc: United Kingdom Flag
Re: Post Your New Tools Of The Trade. [Re: rumfor69]
    #27919604 - 08/26/22 10:49 AM (1 year, 8 months ago)

Yeah my supplier always sends a fresh case if I have any damages.


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Being pissed on does not make you a real man.

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OnlineSmellyhobbitM
Actual Retard
Male User Gallery


Registered: 04/01/22
Posts: 12,833
Loc: Hole
Last seen: 2 seconds
Re: Post Your New Tools Of The Trade. [Re: hamloaf] * 1
    #27919636 - 08/26/22 11:04 AM (1 year, 8 months ago)

I’m thinking I could ultimately save some cash and time in the long run if I bought about 20 glass plates and then a single box of 60 disposable ones for the year. Rotate through the glass ones, then use a disposable for longer term storage when necessary.

But glass Petris are pricey. So probably for a while I’m just gonna use disposable ones.


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A Love Letter to New Growers
A Guide for New Growers
Need Spores? - Sablabs.org

Just because your tub contamed, doesn’t mean your attitude has to contam as well. :mushroom:


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InvisibleHobbit GDF
Deadhead
Male


Registered: 02/14/19
Posts: 3,489
Loc: Terrapin station
Re: Post Your New Tools Of The Trade. [Re: Smellyhobbit] * 1
    #27919651 - 08/26/22 11:18 AM (1 year, 8 months ago)

None are broke yet that I know

I got them from 'southern lab warehouse
For $111.00

Something like that

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InvisibleReverendMyc
succinct is not my forte

Registered: 03/29/19
Posts: 1,847
Trusted Cultivator
Re: Post Your New Tools Of The Trade. [Re: Hobbit GDF] * 7
    #27919675 - 08/26/22 11:44 AM (1 year, 8 months ago)

Officially an empty nester (...again). So now I can set up a lab instead of hauling everything from the garage to the kitchen table and back. Just a start so far, but hoping it means that I can be a  lot more productive going forward.

Plan to split the weekend between scouring and setting up the room and doing some actual cult.


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Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any more
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic



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InvisibleHobbit GDF
Deadhead
Male


Registered: 02/14/19
Posts: 3,489
Loc: Terrapin station
Re: Post Your New Tools Of The Trade. [Re: ReverendMyc]
    #27919707 - 08/26/22 12:10 PM (1 year, 8 months ago)

That looks good

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Offlinerumfor69
Bodhicitta Cultivator
Male


Registered: 08/05/11
Posts: 7,131
Loc: In the Gills
Last seen: 3 hours, 29 minutes
Re: Post Your New Tools Of The Trade. [Re: Hobbit GDF] * 1
    #27932139 - 09/03/22 11:35 AM (1 year, 8 months ago)

So this is just an idea I haven't tested it yet. But those of us in older houses, less control over house air particulate load. I have a theory that contams and dust really get up under the threads of jars and get blown into jars while working in front the flowhood and lifting lids. So I'm thinking of trying these to cover the metal band ring glass gap so they come out of the cooker and remain sterile up under there till you can noc em up

6 Pcs Black Silicone Elastic Bands https://a.co/d/4Qdy75R

They stretch, come in different diameters, 1" width should be sufficient, thin enough to tight fit the contours, and very heat resistant.


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Links For Beginners And Beyond:mspoil:

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OfflineLogicaL ChaosM
Ascension Energy & Alien UFOs
Male User Gallery


Folding@home Statistics
Registered: 05/12/07
Posts: 70,093
Loc: The Inexpressible... Flag
Last seen: 2 days, 39 minutes
Trusted Cultivator
OG Cultivator
Re: Post Your New Tools Of The Trade. [Re: rumfor69]
    #27932346 - 09/03/22 02:18 PM (1 year, 8 months ago)

Great idea! Seal those bands like store-bought bottles.

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Offlinerumfor69
Bodhicitta Cultivator
Male


Registered: 08/05/11
Posts: 7,131
Loc: In the Gills
Last seen: 3 hours, 29 minutes
Re: Post Your New Tools Of The Trade. [Re: LogicaL Chaos] * 5
    #27940004 - 09/08/22 08:17 AM (1 year, 8 months ago)

I pulled the trigger on it found this listing they're cheap, returnable, you get 30 of them the size should fit nice and tightly.



This will seal that underband area keeping it sterile or at least a million times cleaner I can use them on my jar no pours, masters, and g2g receivers. Should really help I think.

https://a.co/d/8i3u6J9


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Links For Beginners And Beyond:mspoil:

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OfflineLowDownDennis
Stranger
 User Gallery
Registered: 07/04/22
Posts: 27
Last seen: 1 year, 5 months
Re: Post Your New Tools Of The Trade. [Re: rumfor69]
    #27945332 - 09/11/22 01:07 PM (1 year, 8 months ago)

I got these to hold desiccant beads to keep in storage jars.



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Offlinerumfor69
Bodhicitta Cultivator
Male


Registered: 08/05/11
Posts: 7,131
Loc: In the Gills
Last seen: 3 hours, 29 minutes
Re: Post Your New Tools Of The Trade. [Re: LowDownDennis]
    #27945375 - 09/11/22 01:32 PM (1 year, 8 months ago)

Should work pretty good. You can always put the beads in womens nylon booties tied in knots too.


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Links For Beginners And Beyond:mspoil:

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Offlinerumfor69
Bodhicitta Cultivator
Male


Registered: 08/05/11
Posts: 7,131
Loc: In the Gills
Last seen: 3 hours, 29 minutes
Re: Post Your New Tools Of The Trade. [Re: rumfor69] * 3
    #27945533 - 09/11/22 03:05 PM (1 year, 8 months ago)

Oooo my silicone bands just got delivered. They fit perfectly! A little tight but over time they should stretch and loosen a little from pressure cooking and be great! Sterile underband!



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Links For Beginners And Beyond:mspoil:

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Offlinerumfor69
Bodhicitta Cultivator
Male


Registered: 08/05/11
Posts: 7,131
Loc: In the Gills
Last seen: 3 hours, 29 minutes
Re: Post Your New Tools Of The Trade. [Re: rumfor69] * 1
    #27945551 - 09/11/22 03:18 PM (1 year, 8 months ago)

Right onto the glass bevel for a perfect fit



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Links For Beginners And Beyond:mspoil:

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OfflineCocaineBuffet
Stranger
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Registered: 08/29/19
Posts: 3,758
Last seen: 2 hours, 28 minutes
Trusted Cultivator
Re: Post Your New Tools Of The Trade. [Re: ReverendMyc]
    #27946042 - 09/11/22 09:04 PM (1 year, 8 months ago)

Quote:

ReverendMyc said:
Officially an empty nester (...again). So now I can set up a lab instead of hauling everything from the garage to the kitchen table and back. Just a start so far, but hoping it means that I can be a  lot more productive going forward.

Plan to split the weekend between scouring and setting up the room and doing some actual cult.




Damn the Herman Miller for the clean bench. You living large.

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Shop: North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Mushroom-Hut Substrate Bags   PhytoExtractum Kratom Powder for Sale   Left Coast Kratom Buy Kratom Extract   Myyco.com Isolated Cubensis Liquid Culture For Sale   OlympusMyco.com Olympus Myco Bulk Substrate   Bridgetown Botanicals CBD Concentrates


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