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Offlineuninc4life2010
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Typically, how many agar transfers are required to achieve a clean mycelial sample?
    #27917604 - 08/25/22 04:31 AM (1 year, 5 months ago)

So, assuming that I'm starting from a drop of spore solution on an agar plate, how many transfers to a new plate are typically required before I can achieve a contamination-free plate that can be used to inoculate grain spawn?

I've been told that spore solution isn't perfectly sterile, so I'm assuming that my first agar plate won't be contamination-free.


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OfflineDERRAYLD
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Re: Typically, how many agar transfers are required to achieve a clean mycelial sample? [Re: uninc4life2010] * 2
    #27917608 - 08/25/22 04:35 AM (1 year, 5 months ago)

It can be anything from 1 to ......

It's ultimately up to the cleanliness of your starting sample be it spores, lc , agar, etc.


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OfflineB Traven
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Re: Typically, how many agar transfers are required to achieve a clean mycelial sample? [Re: DERRAYLD]
    #27917810 - 08/25/22 08:23 AM (1 year, 5 months ago)

I like to multiply plates while transferring. 1 plate becomes 2-3, 2-3 become 4-9, etc.

When I feel good about how a plate looks and decide to send it, there are a dozen other plates from that lineage still in the transfer mill. If that leap to grain works out, then I'll probably start sending the other plates. So, even within one specific culture, one plate could go after three transfers while another goes after six.

I also try my best to catch the leading edge of growth for transfers. When a plate has gotten away from me and fully colonized, I usually leave it to see if I can get some pins for cloning. If it's already a clone culture and I'm not interested in doing that, and it looks OK, then I might send it to grain just because.

Part of it is just my way of keeping plates actively growing. Every transfer buys me a little more time, and spreads out the production of grain masters, without getting into cold storage. I'm also trying to shift into cold-storing grain masters and dehydrated grains, rather than agar plates.

Also, yeah, the main goal is to make sure that you have clean growth. But sequential transfers also constitute genetic manipulation. So there's fun to be had just stretching things out and seeing what happens. My new diversion is letting clone plates pin and taking sequential clones from them. I'm really curious whether I'll see a difference between a single clone and a triple clone in a culture line.


Edited by B Traven (08/25/22 08:32 AM)


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Invisiblebakedbeings
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Re: Typically, how many agar transfers are required to achieve a clean mycelial sample? [Re: B Traven] * 1
    #27917822 - 08/25/22 08:49 AM (1 year, 5 months ago)

its all about how it looks. and its also not just about contam, you want a nice organized plate with no sectoring


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