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Invisiblefungusul
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: Rotnpins] * 1
    #27921792 - 08/27/22 08:33 PM (1 year, 4 months ago)

:threadmonitor:


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OfflineRotnpins
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: Rotnpins] * 1
    #27922242 - 08/28/22 08:38 AM (1 year, 4 months ago)

Here are some pictures to show you guys the growth on a few plates.. they aren't the best pictures,  but you should be able to see tge leading edge to get an idea of the speed of growth.

Plate stared 8/10 (18 days ago):



8/11 (17 days ago):



8/12 (16 days ago):



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OfflineCob
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: Rotnpins]
    #27922326 - 08/28/22 09:48 AM (1 year, 4 months ago)

Damn that is fast! That's cool, I'm going to make a batch and transfer some really slow growing B+ I have. I think transferring it to a less nutritious medium may get it moving.


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Offlinezilker
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: Rotnpins] * 2
    #27924925 - 08/29/22 10:15 PM (1 year, 4 months ago)

The speed of growth on this shit is extremely fast. I currently have 4 "types" of mushrooms growing on WA, each using different methods; spores, substrate, and A2A transfers. Definitely a great clean up tool. When using, be prepared to transfer quickly, as some types & methods are very aggressive growers.

Of the 4 types I've used this with, gourmet seems to grow out faster than cubes.  I know...super scientific with too many details to follow.

Believe it or not, it actually took me 45 minutes to write this post when I realized I'm all over the f'n place, deleted the nonsense, and settled with the KISS strategy.

:drinkingproblem:


Edited by zilker (08/29/22 11:23 PM)


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OfflineChildOfTheMoon
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: zilker] * 1
    #27927725 - 08/31/22 03:46 PM (1 year, 4 months ago)

Thank you so much for this thread :heart: gave it a try last week with a few dirty plates and so far it seems like Iโ€™m getting clean growth out of them all (thereโ€™s two plates with condensation I havenโ€™t looked at) :cool:


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OfflineRotnpins
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: Rotnpins]
    #27927754 - 08/31/22 04:01 PM (1 year, 4 months ago)

Quote:

ChildOfTheMoon said:
Thank you so much for this thread :heart: gave it a try last week with a few dirty plates and so far it seems like Iโ€™m getting clean growth out of them all (thereโ€™s two plates with condensation I havenโ€™t looked at) :cool:





That's awesome! Glad you're seeing good results :super:


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OfflineCob
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: zilker]
    #27928024 - 08/31/22 07:01 PM (1 year, 4 months ago)

Quote:

zilker said:
The speed of growth on this shit is extremely fast. I currently have 4 "types" of mushrooms growing on WA, each using different methods; spores, substrate, and A2A transfers. Definitely a great clean up tool. When using, be prepared to transfer quickly, as some types & methods are very aggressive growers.

Of the 4 types I've used this with, gourmet seems to grow out faster than cubes.  I know...super scientific with too many details to follow.

Believe it or not, it actually took me 45 minutes to write this post when I realized I'm all over the f'n place, deleted the nonsense, and settled with the KISS strategy.

:drinkingproblem:




I wondered about germinating spores on water agar. You've had luck with it you say?

I'm thinking of making my next batch WA and trying some spores out on it. All my current plates are growing pretty slow at 8g LME. Thought about dialing that back a little next time I make nutritious agar plates.


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OfflineRotnpins
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: Cob] * 2
    #27928043 - 08/31/22 07:14 PM (1 year, 4 months ago)

Yeah, I've germinated spores on water agar..

It works, but I don't think the growth is any faster than a nutrient plate..

I've had good luck putting a little less than half my normal nutrient level for germ plates.. I use MYA for my standard plates, but for the germ plates I use half the malt and skip the yeast.


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InvisibleBaba Yaga
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: Rotnpins] * 3
    #27928387 - 08/31/22 11:17 PM (1 year, 4 months ago)

I second that. For a while I'm using agar with 1% LME for everything including germination with not problems. Thinking about lowering it to 0.5% and see how that performs.


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OfflineRusty2096
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: Rotnpins] * 2
    #27933386 - 09/04/22 10:21 AM (1 year, 4 months ago)

WA vs BRF agar experiment

I'm posting here because I will be trying to clean a culture using water agar and I thought it would be fun as I will also compare the results with BRF agar. My logic here is WA could work due to the rapid expansion of the myc looking for nutes and maybe be able to outrun the bacteria. For BRF I was just thinking: if bacterias have a hard time in BRF cakes, maybe it will also have a hard time on BRF agar vs the original PDA plates.

In the picture below, PDA plates 1 and 4 were inoculated from grains coming from a jar noct with a very bacterial LC. As you can see it's still really bad.

I'm hoping the myc on plate 1 will manage top grow past the bacteria (towards where the purple arrow is pointing) to make a simple transfer. That being said I'm realistic and I'm not counting on it so much.

In case it doesn't grow past it, I made transfers from plate 4 to both plates 2 and 3. I used a scalpel to scrape a tiny amount of myc on the grain and then dipped my blade in 2 locations on both plates 2 and 3 (don't worry I did flame sterilize my blade between plates).



Anyways just a fun little experiment of how well it's gonna go to clean the original PDA plate transfering to BRF agar vs WA.

I will post my progress in a few day.

:dancer:


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Currently looking for nothing. You guys who sent me stuff are straight up awesome!. :mushroom2:

We don't own things - things own us.

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Edited by Rusty2096 (09/04/22 10:23 AM)


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OfflineRotnpins
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: Rotnpins] * 2
    #27933396 - 09/04/22 10:27 AM (1 year, 4 months ago)

Thanks for sharing ๐Ÿ‘  I look forward to seeing your results.


For those of you interested in the experiment p9 and I were talking about a few posts ago, I'm going to start the plates during my agar session today.. I'll post the results in a few days.


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OfflineRusty2096
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: Rotnpins]
    #27933414 - 09/04/22 10:34 AM (1 year, 4 months ago)

:popcorn:


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Currently looking for nothing. You guys who sent me stuff are straight up awesome!. :mushroom2:

We don't own things - things own us.

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InvisiblePsicomb
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: Rusty2096] * 1
    #27933444 - 09/04/22 10:43 AM (1 year, 4 months ago)

Cool convo erybody :cool:

Just inoculated water agar plates with 15 different cubensis varieties.  Really hoping they all take a footing :smile: threw the cultures on nutrient plates too in case the water agar gives me trouble for unknown reasons.

I'll post as it plays out.  Planning to use this for the sterilised distilled water storage.


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When we constantly pull things apart trying to see how it works, we may end up with only an understanding of how to destroy something
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InvisibleStipe-n CapMDiscord
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: Rusty2096] * 2
    #27933487 - 09/04/22 11:07 AM (1 year, 4 months ago)

Quote:

Rusty2096 said:
WA vs BRF agar experiment

... For BRF I was just thinking: if bacterias have a hard time in BRF cakes, maybe it will also have a hard time on BRF agar vs the original PDA plates.





So the reason why brf is resistant to bacterial blooms isn't the result of rice flour having an intrinsic antibacterial property to it.

Mycelium that is grown on grains that has been weakened by an infection will be further weakened by the shake prior to spawning; shaking allows for aggressive competitors like bacteria to take hold during this window of opportunity.

Shaking up compromised spawn not only weakens the mycelium but it simultaneously spreads bacteria throughout the grains; when these weakened/infected grains are mixed into a bulk substrate they're now exposed to a water reservoir that fascilitates the bloom of bacteria.

This is why pf tek is the preferred tek when using septic inoculum like ms solution. With pf tek the granular structure of the brf holds bacteria in place while the colony runs through the substrate; fruiting intact pf cakes  keeps the mycelial colony intact because we're no longer shaking and weakening the colony allowing for the infection to not only spread but take advantage of the recovery window that the mycelium needs.

Good luck with your experiment, I don't mean to be overly didactic/pedantic but it seems germaine to point out why brf/PF tek does what it does.


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OfflineRusty2096
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: Stipe-n Cap] * 1
    #27933641 - 09/04/22 12:57 PM (1 year, 4 months ago)

I do appreciate your explicit answer p9. Your BRF cake resistance explanation totally make sense.

I am still very new to all this so sometimes I may not connect the dots fast enough.

I'll keep reading, practicing and experimenting. Also, I love the challenge of cleaning that culture I was told by a few experienced members to give up on. The reason of my perseverance with this project is not to prove them wrong but to learn, experiment and grow in the process while I am waiting for some prints to come in.


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Currently looking for nothing. You guys who sent me stuff are straight up awesome!. :mushroom2:

We don't own things - things own us.

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InvisibleStipe-n CapMDiscord
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: Rusty2096] * 2
    #27933644 - 09/04/22 01:00 PM (1 year, 4 months ago)

That's the name of the game:

"The difference between screwing around and science is writing it down."
-Adam Savage


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OfflineRotnpins
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: Rusty2096]
    #27933704 - 09/04/22 02:00 PM (1 year, 4 months ago)

Quote:

Rusty2096 said:
Also, I love the challenge of cleaning that culture I was told by a few experienced members to give up on. The reason of my perseverance with this project is not to prove them wrong but to learn, experiment and grow in the process while I am waiting for some prints to come in.




There were a lot of plates that I put in my "toss box" before I started working with water agar that I've now had success going back and cleaning up.. It's definitely a game changer for my cleaning process and deciding when a culture is too difficult to clean..

Now my attitude is more "if there is the tiniest piece of mycelium to salvage, it's probably possible to clean it"...hopefully you have a similar experience.


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OfflineRusty2096
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: Rotnpins]
    #27933818 - 09/04/22 03:12 PM (1 year, 4 months ago)

Quote:

That's the name of the game:

"The difference between screwing around and science is writing it down."
-Adam Savage




:takingnotes:


Quote:

Rotnpins said:
Quote:

Rusty2096 said:
Also, I love the challenge of cleaning that culture I was told by a few experienced members to give up on. The reason of my perseverance with this project is not to prove them wrong but to learn, experiment and grow in the process while I am waiting for some prints to come in.




There were a lot of plates that I put in my "toss box" before I started working with water agar that I've now had success going back and cleaning up.. It's definitely a game changer for my cleaning process and deciding when a culture is too difficult to clean..

Now my attitude is more "if there is the tiniest piece of mycelium to salvage, it's probably possible to clean it"...hopefully you have a similar experience.




:takingnotes:


--------------------
Currently looking for nothing. You guys who sent me stuff are straight up awesome!. :mushroom2:

We don't own things - things own us.

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Offlinemy_mycelium
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: Rusty2096]
    #27934077 - 09/04/22 05:55 PM (1 year, 4 months ago)

Hey there shroomies,

Quick question please, I'm trying to grow B+ on agar from a print. This looks pretty good to me but want to check this isn't some weird contam that I'm not aware of. Am I ok here?

Also, I'm going to do a couple of agar to agar transfers, am I close to the point at which I should do the transfers?


Thanks alot for all the advice!




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OfflineRusty2096
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: my_mycelium] * 1
    #27934089 - 09/04/22 06:03 PM (1 year, 4 months ago)

Quote:

my_mycelium said:
Hey there shroomies,

Quick question please, I'm trying to grow B+ on agar from a print. This looks pretty good to me but want to check this isn't some weird contam that I'm not aware of. Am I ok here?

Also, I'm going to do a couple of agar to agar transfers, am I close to the point at which I should do the transfers?


Thanks alot for all the advice!







Welcome to the Shroomery!

In the spirit of not hijacking this water agar thread (unless you did use WA and forgot to mention it) maybe you should post there instead --> AGAR ENVY


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Currently looking for nothing. You guys who sent me stuff are straight up awesome!. :mushroom2:

We don't own things - things own us.

Semi-solid liquid culture (SSLC)


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