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OfflineSoloist
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Registered: 02/10/22
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: rumfor69]
    #28274752 - 04/12/23 04:53 PM (9 months, 11 days ago)

Quote:

rumfor69 said:

Also if you have enmeshed bacteria or mold that feeds off and with the mycelium in a symbiotic way WA wont help you at all.





What do you do when this happens?
I just transferred off water agar back onto wbs agar..
All my cups look like this.




I donโ€™t know what to do next.


--------------------
Embrace your darkness,
For without it,
Your light can never
truly exist.

                  ๐ŸŒ•๐ŸŒ–๐ŸŒ—๐ŸŒ˜๐ŸŒ‘๐ŸŒ’๐ŸŒ“๐ŸŒ”๐ŸŒ•
                          The Earth And I
89g fresh Gymnopilus subspectibilis
Rapรฉh Crafters
Trade and wish list
๐Ÿ„๐Ÿ‘€MO๐Ÿ„๐Ÿ‘€
Itโ€™s time to ghost this placeโœŒ๐Ÿป


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Offlinerumfor69
Bodhicitta Cultivator
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Registered: 08/05/11
Posts: 6,767
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: Soloist] * 1
    #28274878 - 04/12/23 06:59 PM (9 months, 11 days ago)

I wanna help but those pics aren't good enough. You can't use a flash. Try using a white LED flashlight and taking the pic from different close up angles till you get something better. Also take some in a dark room with the flashlight back lighting it to show its translucency.

Stuff like this:


And as far as enmeshed go there's no way to tell until you goto grain and even then can only be sure if your technique is flawless and wasn't an error on your part. And FYI these cultures in these pics were enmeshed with bacteria and would ruin any grain jar they went in. The only fix was to go back to spores and start again.


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Invisiblemyc_ousin_vinny
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: rumfor69]
    #28274893 - 04/12/23 07:16 PM (9 months, 11 days ago)

Damn, that first plate looks close to perfect too.


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Offlinerumfor69
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: myc_ousin_vinny]
    #28275081 - 04/12/23 09:13 PM (9 months, 11 days ago)

They continued to be transferred and transferred even to WA and back again. They eventually all looked perfect. All I could do was blame the grain, or it's prep, or the pc cycle.

But after doing tests with blank grain jars and many conversations with Stipe and others I concluded they were enmeshed with bacteria.
Next bacterial tubs that fruited I took some swabs, put them directly to agar, they were perfect by T1, went to grain, everything was clean, already cloned 6 monsters.

The new dishes don't look anything like those enmeshed ones. I have a theory as to what characteristics can be seen in these pics to watch out for but it's just a guess. And can be super subtle like in the first pic.


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OfflineAspectOfTheCreator
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: rumfor69]
    #28276236 - 04/13/23 05:31 PM (9 months, 10 days ago)

Put a speck of Natalensis myc from a PF tek jar onto a water agar plate. It behaved unlike other agar to water agar transfers I have done.

After a week, I haven't gotten that fine translucent growth I've come to associate with water agar. Not sure if it's a Natalensis thing or just what happens when going pf tek to water agar.









Have several other varities of pf tek myc to water agar going. Will have to see if they behave similarly. Perhaps it's just Natalensis being the monster that it is.

Regardless, is it too soon to transfer? Should I wait longer? Has the water agar done its job?


Edited by AspectOfTheCreator (04/13/23 06:28 PM)


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Offlinerumfor69
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: AspectOfTheCreator]
    #28277297 - 04/14/23 11:35 AM (9 months, 9 days ago)

I'd probably let them grow a little bit more but they look good


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OfflineSoloist
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Registered: 02/10/22
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: rumfor69]
    #28277462 - 04/14/23 01:34 PM (9 months, 9 days ago)

So I posted these in other spots and a TC said it looked like weak myc, due to its filamentous nature. You can see it on the edges.
I had the same haze on the germ plates, and I thought it was weak myc at that point tooโ€ฆ but another member diagnosed it as bacterial and being a noob, on to WA they went.
Here we are at t2.
Iโ€™m going to trust the TC on this one (Nichrome).
I may be just a noob but Iโ€™ve done hella research and some intentional contamination test runs before I tried to germ, so I knew what to look for. This haze doesnโ€™t line up with any of the other obvious bacterial contams Iโ€™ve seen either.

All the donor cups so far smell nice and mushroomy too.
I appreciate all the input. Just gonna let these guys run till itโ€™s t3 time.


--------------------
Embrace your darkness,
For without it,
Your light can never
truly exist.

                  ๐ŸŒ•๐ŸŒ–๐ŸŒ—๐ŸŒ˜๐ŸŒ‘๐ŸŒ’๐ŸŒ“๐ŸŒ”๐ŸŒ•
                          The Earth And I
89g fresh Gymnopilus subspectibilis
Rapรฉh Crafters
Trade and wish list
๐Ÿ„๐Ÿ‘€MO๐Ÿ„๐Ÿ‘€
Itโ€™s time to ghost this placeโœŒ๐Ÿป


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Offlinedyel
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: Rotnpins] * 1
    #28278555 - 04/15/23 08:10 AM (9 months, 8 days ago)

giving  this a shot cloning nasty supermarket mushrooms
nice writeup :rockon:


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Invisiblemyc_ousin_vinny
Keeping_It_Real
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Registered: 04/29/20
Posts: 1,415
Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: Soloist]
    #28278634 - 04/15/23 09:05 AM (9 months, 8 days ago)

Quote:

Soloist said:
So I posted these in other spots and a TC said it looked like weak myc, due to its filamentous nature. You can see it on the edges.
I had the same haze on the germ plates, and I thought it was weak myc at that point tooโ€ฆ but another member diagnosed it as bacterial and being a noob, on to WA they went.
Here we are at t2.
Iโ€™m going to trust the TC on this one (Nichrome).
I may be just a noob but Iโ€™ve done hella research and some intentional contamination test runs before I tried to germ, so I knew what to look for. This haze doesnโ€™t line up with any of the other obvious bacterial contams Iโ€™ve seen either.

All the donor cups so far smell nice and mushroomy too.
I appreciate all the input. Just gonna let these guys run till itโ€™s t3 time.




Did you mean to post pics?


--------------------
Bod's Easy AF Search Engine TEK

Let's stay focused on what works consistently.

"Sitting in a bunker here behind my wall..."


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OfflineSoloist
Indigenous Human

Registered: 02/10/22
Posts: 922
Loc: Suburban hell
Last seen: 12 hours, 22 minutes
Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: myc_ousin_vinny]
    #28278698 - 04/15/23 10:07 AM (9 months, 8 days ago)

Nah, replying to comments about a post a few up.


--------------------
Embrace your darkness,
For without it,
Your light can never
truly exist.

                  ๐ŸŒ•๐ŸŒ–๐ŸŒ—๐ŸŒ˜๐ŸŒ‘๐ŸŒ’๐ŸŒ“๐ŸŒ”๐ŸŒ•
                          The Earth And I
89g fresh Gymnopilus subspectibilis
Rapรฉh Crafters
Trade and wish list
๐Ÿ„๐Ÿ‘€MO๐Ÿ„๐Ÿ‘€
Itโ€™s time to ghost this placeโœŒ๐Ÿป


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Offlinex7x_x7x
x7x, my problem child.
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Folding@home Statistics
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: Rotnpins] * 1
    #28284236 - 04/18/23 06:42 PM (9 months, 5 days ago)

could spores grown in water agar or is for transfers only?

great work.


--------------------
cultivando en la miseria

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x7x_x7x@shroomery.org

carl_jung_in_lsd@yahoo.com

koh samui and oak ridge are my favourite strains


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Offlinerumfor69
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Registered: 08/05/11
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: x7x_x7x] * 1
    #28284493 - 04/18/23 09:00 PM (9 months, 5 days ago)

Quote:

x7x_x7x said:
could spores grown in water agar or is for transfers only?

great work.




I believe some earlier in the thread germinated spores on WA. But it's not necessary. WA is just a tool you can use to try to get away from stubborn bacteria. Otherwise it's pointless to use really.


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Offlinex7x_x7x
x7x, my problem child.
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: rumfor69]
    #28292056 - 04/23/23 07:06 PM (9 months, 10 hours ago)

thanks! just made six plates. the most interesting media after tomatoes agar.

:discorex:


Quote:

rumfor69 said:
Quote:

x7x_x7x said:
could spores grown in water agar or is for transfers only?

great work.




I believe some earlier in the thread germinated spores on WA. But it's not necessary. WA is just a tool you can use to try to get away from stubborn bacteria. Otherwise it's pointless to use really.




--------------------
cultivando en la miseria

SuctoSporeยฎ Pictorial Tek


x7x_x7x@shroomery.org

carl_jung_in_lsd@yahoo.com

koh samui and oak ridge are my favourite strains


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OfflineSoloist
Indigenous Human

Registered: 02/10/22
Posts: 922
Loc: Suburban hell
Last seen: 12 hours, 22 minutes
Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: x7x_x7x]
    #28611778 - 01/06/24 01:52 PM (21 days, 14 hours ago)

So Iโ€™ve had this Water agar for about 9 months, maybe out of the fridge for the last 2 months or soโ€ฆ

Held it up to a light for shits and gigsโ€ฆand saw these.





3 transparent discs of different sizes.
They have a mirrored sheen at a certain angle and a slight convex shape.
Obviously some sort of contam but nothing else visible.
Really interesting.

You guys ever see anything like this before?


--------------------
Embrace your darkness,
For without it,
Your light can never
truly exist.

                  ๐ŸŒ•๐ŸŒ–๐ŸŒ—๐ŸŒ˜๐ŸŒ‘๐ŸŒ’๐ŸŒ“๐ŸŒ”๐ŸŒ•
                          The Earth And I
89g fresh Gymnopilus subspectibilis
Rapรฉh Crafters
Trade and wish list
๐Ÿ„๐Ÿ‘€MO๐Ÿ„๐Ÿ‘€
Itโ€™s time to ghost this placeโœŒ๐Ÿป


Edited by Soloist (01/06/24 01:53 PM)


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