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OfflineNuclearTidalWave
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Registered: 09/07/16
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: rumfor69]
    #28160574 - 01/27/23 10:08 PM (1 year, 6 hours ago)

I concur


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OfflineSirPsycho
Purple Belt in Google-Fu
I'm a teapot User Gallery


Registered: 01/01/20
Posts: 6,900
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Trusted Cultivator
Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: NuclearTidalWave] * 1
    #28162430 - 01/29/23 06:49 AM (11 months, 22 days ago)

I miss Rotn. Anyone else?

Sure hope he's doing ok.


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:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  ๐Ÿ…‘๐Ÿ…ž๐Ÿ…ฃ๐Ÿ…ฃ๐Ÿ…›๐Ÿ…” ๐Ÿ…–๐Ÿ…๐Ÿ…๐Ÿ…–

"Mist your balls and fan your asshole" - Pandaskis, 2023


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InvisibleShrimps
Traveler
I'm a teapot

Registered: 10/13/22
Posts: 2,501
Loc: Under the sea
Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: SirPsycho] * 1
    #28162431 - 01/29/23 06:53 AM (11 months, 22 days ago)

I just thought of him yesterday, whats up with him? Sending some good energy towards him!


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I am not crazy, I prefer the term mentally hillarious.

Agar - The way to go!
Clean spawn checklist
Proper Surface Moisture
Recognizing and dealing with contamination

๐Ÿ…ƒ ๐Ÿ„ด ๐Ÿ„ฐ ๐Ÿ„ผ    ๐Ÿ„ฒ ๐Ÿ„ป ๐Ÿ„ธ ๐Ÿ„ฝ ๐Ÿ„ถ ๐Ÿ…† ๐Ÿ… ๐Ÿ„ฐ ๐Ÿ„ฟ


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Offlinerumfor69
Bodhicitta Cultivator
Male


Registered: 08/05/11
Posts: 6,767
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: Shrimps] * 2
    #28164266 - 01/30/23 11:56 AM (11 months, 21 days ago)

I've thought about his absence also. Hopefully he's good.


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InvisibleHILLBILLY OUTLAWS
Above And Beyond!
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Registered: 04/21/22
Posts: 2,852
Loc: Luckenbach Texas
Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: Rotnpins]
    #28164581 - 01/30/23 03:46 PM (11 months, 21 days ago)

I PMd him over the last week or so to check in on him with no reply.  Been offline since just before Christmas.
Defiantly a presence that has been greatly missed.


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๐Ÿ…ƒ ๐Ÿ„ด ๐Ÿ„ฐ ๐Ÿ„ผ    ๐Ÿ„ฒ ๐Ÿ„ป ๐Ÿ„ธ ๐Ÿ„ฝ ๐Ÿ„ถ ๐Ÿ…† ๐Ÿ… ๐Ÿ„ฐ ๐Ÿ„ฟ
TEAM SPREAD THE LOVE!
Smellyhobbit said:
Embarrassment and bashfulness are leeches on your ability to learn.


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OfflineSeaSalt
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Registered: 12/08/21
Posts: 495
Loc: PNW WWA
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: HILLBILLY OUTLAW]
    #28224107 - 03/10/23 05:23 PM (10 months, 13 days ago)

No sign of Rottn?
I've had several varieties of cubes and woodlovers on water agar since Last fall. Unfortunately, I don't know how to read the mycelium. Some have white rings, some have cloudy halos, some have brown rings.
I'm never confident taking a transfer because I don't know what I'm looking at. At what point are you sure the mycellium has out run the bacteria?

Taking pictures of water agar is a challenge in itself. Unless, a person is set up with back lighting.
Does anyone know of another thread that shows mycelium on water agar?


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Offlinerumfor69
Bodhicitta Cultivator
Male


Registered: 08/05/11
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: SeaSalt]
    #28224204 - 03/10/23 06:12 PM (10 months, 13 days ago)

I'm not sure. But the theory here is that the bacteria doesn't grow out from the transferred piece. So if you grab some myce that has grown out towards the edge your going to get something clean. I suggest transferring while back lighting in a dark room under magnification if possible.

I miss Rotn


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InvisibleEgon_Spengler
Ever lurkin never workin
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Registered: 04/02/22
Posts: 2,294
Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: rumfor69]
    #28224231 - 03/10/23 06:21 PM (10 months, 13 days ago)

Quote:

rumfor69 said:

I miss Rotn




Me too

:feelsbatman:


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InvisibleHILLBILLY OUTLAWS
Above And Beyond!
Male


Registered: 04/21/22
Posts: 2,852
Loc: Luckenbach Texas
Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: Rotnpins]
    #28224359 - 03/10/23 07:31 PM (10 months, 13 days ago)

We wish you were here!!



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๐Ÿ…ƒ ๐Ÿ„ด ๐Ÿ„ฐ ๐Ÿ„ผ    ๐Ÿ„ฒ ๐Ÿ„ป ๐Ÿ„ธ ๐Ÿ„ฝ ๐Ÿ„ถ ๐Ÿ…† ๐Ÿ… ๐Ÿ„ฐ ๐Ÿ„ฟ
TEAM SPREAD THE LOVE!
Smellyhobbit said:
Embarrassment and bashfulness are leeches on your ability to learn.


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OfflineSmellyhobbit
Actual Retard
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Registered: 04/01/22
Posts: 11,144
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: HILLBILLY OUTLAW] * 1
    #28224501 - 03/10/23 09:44 PM (10 months, 13 days ago)

Imagine writing a song so fucking good. Imagine putting on a show and your song being so beloved that the audience is either totally fucking silent while you play it or they all sing it with you perfectly.

God. I wish.

Rotn please come home. Iโ€™m a wreck without you.


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A Love Letter to New Growers
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OfflineSeaSalt
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Registered: 12/08/21
Posts: 495
Loc: PNW WWA
Last seen: 4 months, 24 days
Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: Smellyhobbit] * 1
    #28224599 - 03/11/23 01:11 AM (10 months, 13 days ago)

Quote:

Smellyhobbit said:
Imagine writing a song so fucking good. Imagine putting on a show and your song being so beloved that the audience is either totally fucking silent while you play it or they all sing it with you perfectly.

God. I wish.

Rotn please come home. Iโ€™m a wreck without you.



Me too.
Tomorrow I'll try and photograph my plates.
I'm hoping you guys can help me wing it.


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OfflineSoloist
Indigenous Human

Registered: 02/10/22
Posts: 922
Loc: Suburban hell
Last seen: 12 hours, 21 minutes
Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: SeaSalt]
    #28225306 - 03/11/23 03:37 PM (10 months, 12 days ago)

This thread is an amazing resource.
Noccโ€™d up a few deli cups with a TYC print labeled โ€œdirtyโ€ the other dayโ€ฆ I could see bacteria on day two haha, over half of them.
Nice to have this in my pocket.
Thanks to all you guys!


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Embrace your darkness,
For without it,
Your light can never
truly exist.

                  ๐ŸŒ•๐ŸŒ–๐ŸŒ—๐ŸŒ˜๐ŸŒ‘๐ŸŒ’๐ŸŒ“๐ŸŒ”๐ŸŒ•
                          The Earth And I
89g fresh Gymnopilus subspectibilis
Rapรฉh Crafters
Trade and wish list
๐Ÿ„๐Ÿ‘€MO๐Ÿ„๐Ÿ‘€
Itโ€™s time to ghost this placeโœŒ๐Ÿป


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Invisiblemyc_ousin_vinny
Keeping_It_Real
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Registered: 04/29/20
Posts: 1,415
Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: Soloist]
    #28233405 - 03/17/23 08:42 AM (10 months, 6 days ago)

I'm making some water agar today. I've got some Natalensis and Albino Cambodian to germ.

:seriousthankyou:

Rotnpins you still out there?


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Let's stay focused on what works consistently.

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Offlineherbstation
Child


Registered: 01/05/23
Posts: 217
Last seen: 1 day, 17 hours
Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: myc_ousin_vinny]
    #28233444 - 03/17/23 09:09 AM (10 months, 6 days ago)

I just got some cultures and spore syringes. Can I use water agar or should I add a tiny bit of karo? I have been making potato flake agar and it works but I inevitably get slime.

Cheers
Herb


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Expanding my mind until I can join the collective


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Offlinerumfor69
Bodhicitta Cultivator
Male


Registered: 08/05/11
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: herbstation]
    #28233540 - 03/17/23 10:53 AM (10 months, 6 days ago)

Usually water agar is a tool used when you have a bacterial infected culture. While you can probably germinate spores on it. I would personally germinate on regular agar first.


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Offlineherbstation
Child


Registered: 01/05/23
Posts: 217
Last seen: 1 day, 17 hours
Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: rumfor69]
    #28233612 - 03/17/23 11:51 AM (10 months, 6 days ago)

Thank you. I have a contaminated culture I would like to save as well as some clean ones so this is perfect.


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Expanding my mind until I can join the collective


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Offlinerumfor69
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: herbstation]
    #28233629 - 03/17/23 12:02 PM (10 months, 6 days ago)

Just remember this tek wont help with mold contamination cuz it's mycelium and will grow on WA. This tool is only for bacteria.


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OfflineSeaSalt
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Registered: 12/08/21
Posts: 495
Loc: PNW WWA
Last seen: 4 months, 24 days
Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: rumfor69]
    #28233782 - 03/17/23 01:26 PM (10 months, 6 days ago)

Quote:

rumfor69 said:
Just remember this tek wont help with mold contamination cuz it's mycelium and will grow on WA. This tool is only for bacteria.



Any idea what white mold looks like on Water Agar?


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Offlinerumfor69
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Male


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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: SeaSalt]
    #28233791 - 03/17/23 01:30 PM (10 months, 6 days ago)

It's probably gonna look real wispy and difficult if not impossible to distinguish from the mycelium you want. If you think a culture has mold, regular agar would be better at feeding it so it shows stronger characteristics that allow you to transfer away from it easier. Just my opinion.


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OfflineSeaSalt
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: rumfor69]
    #28233811 - 03/17/23 01:47 PM (10 months, 6 days ago)

Quote:

rumfor69 said:
It's probably gonna look real wispy and difficult if not impossible to distinguish from the mycelium you want. If you think a culture has mold, regular agar would be better at feeding it so it shows stronger characteristics that allow you to transfer away from it easier. Just my opinion.



Well, I have a few plates that develop wispy mycilium around 60+% colonization.
One of these days I'll figure out how to take pictures of WA from my phone. Trying to get back lighting and a background that doesn't show light fixtures/wallpaper/windows etc...has proved frustrating.


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