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OfflineRotnpins
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: Bigworm]
    #28020273 - 10/27/22 08:30 PM (1 year, 2 months ago)

Hopefully the results will be encouraging, but even if they're not I plan on running more jars.. one bad jar doesn't really mean anything.. I could have sent too much of the plate and not cut far enough from the center..

I've got 3 plates that are knotting up, so I'm saving them to see if it's actually possible to get a plate pin on WA.. but I'll be sending a bunch of my other plats soon.


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Offlinehazyhorse
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: Rotnpins] * 4
    #28020279 - 10/27/22 08:32 PM (1 year, 2 months ago)

for real, youโ€™re doing some amazing stuff for the community with this thread & itโ€™s all great info to have. the more we know the more we can apply our knowledge & streamline shit.

but the way we treat plants & fungus is just so hilarious on a meta level. cutting it down to a few cells with a hot knife & forcing it to do all this stress response shit, shaking it up as itโ€™s trying to colonize & consume nutrients, breaking it up & forcing it to recolonize & weโ€™re out here like โ€œoh yeah, the myc LOVES itโ€ lol


--------------------
you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

๐Ÿ…ƒ ๐Ÿ„ด ๐Ÿ„ฐ ๐Ÿ„ผ    ๐Ÿ„ฒ ๐Ÿ„ป ๐Ÿ„ธ ๐Ÿ„ฝ ๐Ÿ„ถ ๐Ÿ…† ๐Ÿ… ๐Ÿ„ฐ ๐Ÿ„ฟ


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OfflineSirPsycho
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: hazyhorse] * 3
    #28020297 - 10/27/22 08:38 PM (1 year, 2 months ago)

Quote:

hazyhorse said:
for real, youโ€™re doing some amazing stuff for the community with this thread & itโ€™s all great info to have. the more we know the more we can apply our knowledge & streamline shit.

but the way we treat plants & fungus is just so hilarious on a meta level. cutting it down to a few cells with a hot knife & forcing it to do all this stress response shit, shaking it up as itโ€™s trying to colonize & consume nutrients, breaking it up & forcing it to recolonize & weโ€™re out here like โ€œoh yeah, the myc LOVES itโ€ lol



Maybe the myc likes it rough, you don't know


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  ๐Ÿ…‘๐Ÿ…ž๐Ÿ…ฃ๐Ÿ…ฃ๐Ÿ…›๐Ÿ…” ๐Ÿ…–๐Ÿ…๐Ÿ…๐Ÿ…–

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OfflineRotnpins
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: hazyhorse]
    #28020304 - 10/27/22 08:40 PM (1 year, 2 months ago)

I completely agree.. I've actually had the same thoughts before.. a lot of things done in the name of scientific progress tend to have this effect..

Hopefully, since I've been running experiments on the poor mycelium, I'll relieve any suffering when I send the plates to grain and allow them to flourish :mushroom2:


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Offlinehazyhorse
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: Rotnpins] * 2
    #28020334 - 10/27/22 08:49 PM (1 year, 2 months ago)

oh yeah, itโ€™s much easier to get data if you throw ethics out the window. luckily fungus doesnโ€™t have a seat at the UNโ€ฆ yet

and after that beautiful flush they can experience the sweet release of death as their progeny are cloned & tortured. the circle of life :inlove3:


--------------------
you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

๐Ÿ…ƒ ๐Ÿ„ด ๐Ÿ„ฐ ๐Ÿ„ผ    ๐Ÿ„ฒ ๐Ÿ„ป ๐Ÿ„ธ ๐Ÿ„ฝ ๐Ÿ„ถ ๐Ÿ…† ๐Ÿ… ๐Ÿ„ฐ ๐Ÿ„ฟ


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OfflineSmellyhobbit
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: hazyhorse]
    #28020340 - 10/27/22 08:51 PM (1 year, 2 months ago)

Quote:

hazyhorse said:
oh yeah, itโ€™s much easier to get data if you throw ethics out the window. luckily fungus doesnโ€™t have a seat at the UNโ€ฆ yet




That youโ€™re aware of ๐Ÿ‘€


--------------------
A Love Letter to New Growers
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Growth 2023 - A Year In Review

Grow more shrooms. Eat more ass. :mushroom:



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Offlinehazyhorse
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: Smellyhobbit]
    #28020495 - 10/27/22 10:36 PM (1 year, 2 months ago)

.
Quote:

Smellyhobbit said:
That youโ€™re aware of ๐Ÿ‘€




tbh would not be shocked at all to find out fungus has been slowly controlling the entire human sociopolitical sphere. they already cultivate every other aspect of earth


--------------------
you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

๐Ÿ…ƒ ๐Ÿ„ด ๐Ÿ„ฐ ๐Ÿ„ผ    ๐Ÿ„ฒ ๐Ÿ„ป ๐Ÿ„ธ ๐Ÿ„ฝ ๐Ÿ„ถ ๐Ÿ…† ๐Ÿ… ๐Ÿ„ฐ ๐Ÿ„ฟ


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InvisibleBaba Yaga
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: hazyhorse] * 4
    #28020539 - 10/27/22 11:25 PM (1 year, 2 months ago)

Honestly using water agar is like cheating, where is the craft and art in this? No need for taking great care to cut the tiniest sample over and over again just to find out there is still a contam riding along. I was throwing this pin onto a water agar plate and taking transfers when it had barely grown out 10mm just to get 4 pristine clean plates on the first try and I could probably have gone straight to LC with this making this whole thing soooooooo easy boooooooring. You young folks are missing out on a painful but character building experience.




What challenge has life got ready next? Painting by numbers maybe?

This is utterly ridiculous. :oldman::lol:


Edited by Baba Yaga (10/27/22 11:39 PM)


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Offlinehazyhorse
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: Baba Yaga] * 1
    #28020542 - 10/27/22 11:27 PM (1 year, 2 months ago)

bad ass dude!! im currently trying this on some cultures that look weird & am excited to see the results

has anyone tried germinating spores on WA yet? didnโ€™t feel like digging through the thread at the moment


--------------------
you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

๐Ÿ…ƒ ๐Ÿ„ด ๐Ÿ„ฐ ๐Ÿ„ผ    ๐Ÿ„ฒ ๐Ÿ„ป ๐Ÿ„ธ ๐Ÿ„ฝ ๐Ÿ„ถ ๐Ÿ…† ๐Ÿ… ๐Ÿ„ฐ ๐Ÿ„ฟ


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InvisibleEgon_Spengler
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: Rotnpins] * 1
    #28020751 - 10/28/22 05:53 AM (1 year, 2 months ago)

Quote:

hazyhorse said:
bad ass dude!! im currently trying this on some cultures that look weird & am excited to see the results

has anyone tried germinating spores on WA yet? didnโ€™t feel like digging through the thread at the moment




TLDR; likely too slow to be useful.
Quote:

Rotnpins said:
Quote:

baz said:
Can you germinate spores on water agar?




I haven't tried to do it, but I may toss some spores on a plate later and post the results ๐Ÿค” 

Personally, I like to use plates that have slightly less nutes and agar than my normal plates when making plates specifically for germination... using less agar will make the agar softer and there will be more water to help hydrate the spores.. using less nutrients will help the plate colonize faster, giving you quick growth to transfer to a new plate.

Sometimes you'll see spores germinate in a non nutritional syringe, so I think its possible the plate would germinate.. however I suspect that anything that does germinate will be thin and whispy and hard to distinguish what, if anything, would be worth transferring.

My recommendation for now would be to use nutrient plates for germination.. this will give the spores energy to germinate, and the mycelium will probably be easier to identify.. but I'll post my findings after I give it a try.




:disco:


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OfflineRotnpins
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: Baba Yaga] * 2
    #28020803 - 10/28/22 07:12 AM (1 year, 2 months ago)

:laugh2: you're absolutely right.. if used as your primary method of cleaning, WA does take the sport out of it.. but then again, so do PF pucks and the jossex poke :lol: (not aimed at Faht, he's a total beast that takes things to a whole different level with his oven bags and notoriously clean swabs, etc)

WA has a time and place in this hobby, but if it's used as a crutch, it will definitely take the fun out of things.



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Offlinehazyhorse
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: Rotnpins] * 1
    #28021070 - 10/28/22 10:34 AM (1 year, 2 months ago)

gonna start spitting on my plates just to have a challenge again


--------------------
you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

๐Ÿ…ƒ ๐Ÿ„ด ๐Ÿ„ฐ ๐Ÿ„ผ    ๐Ÿ„ฒ ๐Ÿ„ป ๐Ÿ„ธ ๐Ÿ„ฝ ๐Ÿ„ถ ๐Ÿ…† ๐Ÿ… ๐Ÿ„ฐ ๐Ÿ„ฟ


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OnlineScrewup
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: hazyhorse] * 1
    #28021094 - 10/28/22 10:47 AM (1 year, 2 months ago)

Quote:

hazyhorse said:
gonna start spitting on my plates just to have a challenge again



From which orifice on your body?


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Offlinerumfor69
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: Screwup] * 1
    #28021097 - 10/28/22 10:48 AM (1 year, 2 months ago)

Quote:

Screwup said:
Quote:

hazyhorse said:
gonna start spitting on my plates just to have a challenge again



From which orifice on your body?




All of them, gotta have consistency in experimentation


--------------------

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OfflineRotnpins
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: rumfor69]
    #28021195 - 10/28/22 11:41 AM (1 year, 2 months ago)

Quote:

rumfor69 said:
Quote:

Screwup said:
Quote:

hazyhorse said:
gonna start spitting on my plates just to have a challenge again



From which orifice on your body?




All of them, gotta have consistency in experimentation




:notards:

just kidding.. that shit made me laugh :lol:


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Offlinehazyhorse
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: rumfor69]
    #28021219 - 10/28/22 11:49 AM (1 year, 2 months ago)

Quote:

rumfor69 said:
Quote:

Screwup said:
Quote:

hazyhorse said:
gonna start spitting on my plates just to have a challenge again



From which orifice on your body?




All of them, gotta have consistency in experimentation




rum gets it, itโ€™s all in the name of

:havesomescience:


--------------------
you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

๐Ÿ…ƒ ๐Ÿ„ด ๐Ÿ„ฐ ๐Ÿ„ผ    ๐Ÿ„ฒ ๐Ÿ„ป ๐Ÿ„ธ ๐Ÿ„ฝ ๐Ÿ„ถ ๐Ÿ…† ๐Ÿ… ๐Ÿ„ฐ ๐Ÿ„ฟ


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OfflineSirPsycho
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: Rotnpins] * 1
    #28021225 - 10/28/22 11:52 AM (1 year, 2 months ago)

Quote:

Rotnpins said:
WA has a time and place in this hobby, but if it's used as a crutch, it will definitely take the fun out of things.




Clean cultures are pretty fun. :shrug:


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  ๐Ÿ…‘๐Ÿ…ž๐Ÿ…ฃ๐Ÿ…ฃ๐Ÿ…›๐Ÿ…” ๐Ÿ…–๐Ÿ…๐Ÿ…๐Ÿ…–

"Mist your balls and fan your asshole" - Pandaskis, 2023


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Offlinehazyhorse
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: SirPsycho]
    #28021258 - 10/28/22 12:06 PM (1 year, 2 months ago)

:whathesaid:

also is it really a crutch just for making things easier? itโ€™s a lot less fun dealing with contamination problems


--------------------
you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

๐Ÿ…ƒ ๐Ÿ„ด ๐Ÿ„ฐ ๐Ÿ„ผ    ๐Ÿ„ฒ ๐Ÿ„ป ๐Ÿ„ธ ๐Ÿ„ฝ ๐Ÿ„ถ ๐Ÿ…† ๐Ÿ… ๐Ÿ„ฐ ๐Ÿ„ฟ


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OfflineRotnpins
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: hazyhorse]
    #28021285 - 10/28/22 12:16 PM (1 year, 2 months ago)

Quote:

hazyhorse said:
:whathesaid:

also is it really a crutch just for making things easier? itโ€™s a lot less fun dealing with contamination problems




Yeah.. but someone mentioned earlier automatically doing T1 WA plates.. then all the T2s would be clean, or you could even just send the T1s to grain...

one of my favorite things about this hobby is the agar work .. if you always use WA, and don't just use it for the tricky cultures that won't clean up or are really contaminated, doesn't it kinda take a lot of the fun out of good old fashioned agar cleaning?

If you use it all the time, it kinda makes me think of when I'd use a cheat code on a video game when I was a kid... I'd get bored of the game really fast..

If used once in a while, it's more like using a "potion" or "power-up"... you can still enjoy the game :wink:


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OnlineScrewup
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Re: Let's talk about Water Agar!! ๐Ÿ’ง [Re: Rotnpins]
    #28021288 - 10/28/22 12:18 PM (1 year, 2 months ago)

Gotta say this feels like some boomer ass shit lads. Sure you could get a nice desk job but wouldnโ€™t you like to destroy your body working construction for 20 years? Maybe Iโ€™d hate agar less if I had a FFU thoughโ€ฆ


Edited by Screwup (10/28/22 12:18 PM)


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