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rumfor69
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Re: Let's talk about Water Agar!! 💧 [Re: Rotnpins] 1
#27901299 - 08/12/22 08:20 PM (1 year, 5 months ago) |
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Nice write up I could have definitely used this many times in the past. Is the reason to use 7g agar instead of 10g so that it's a little softer?
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rumfor69
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Re: Let's talk about Water Agar!! 💧 [Re: Psicomb] 1
#27913600 - 08/22/22 12:16 PM (1 year, 5 months ago) |
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Quote:
Psicomvb said: transfer wedges from those into vials of sterilized distilled water for long term storage.
I always thought about doing this rather than making slants but wasn't sure how it'd work.
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rumfor69
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Re: Let's talk about Water Agar!! 💧 [Re: Rotnpins] 1
#27913605 - 08/22/22 12:20 PM (1 year, 5 months ago) |
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I'm curious if it would starve without any nutrients in long term storage.
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rumfor69
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Re: Let's talk about Water Agar!! 💧 [Re: Rotnpins] 1
#27913632 - 08/22/22 12:41 PM (1 year, 5 months ago) |
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That'd be awesome
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rumfor69
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Re: Let's talk about Water Agar!! 💧 [Re: Rotnpins] 1
#27913644 - 08/22/22 12:52 PM (1 year, 5 months ago) |
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rumfor69
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Re: Let's talk about Water Agar!! 💧 [Re: Psicomb] 1
#27914106 - 08/22/22 06:54 PM (1 year, 5 months ago) |
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Quote:
Psicomvb said:
Quote:
rumfor69 said: I'm curious if it would starve without any nutrients in long term storage.
Only one way to find out 
I just hate slants .. They never come out the way I want them to.
Still gonna make some just in case anyway but I pray these are my last slants 4 evaaa
I never tried slants cause I figured it wouldn't come out right for me. I've always just made super thick no pours that last forever in the fridge.
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rumfor69
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Re: Let's talk about Water Agar!! 💧 [Re: Rusty2096]
#27993001 - 10/10/22 11:12 PM (1 year, 3 months ago) |
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rumfor69
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Re: Let's talk about Water Agar!! 💧 [Re: Rusty2096]
#27994216 - 10/11/22 06:40 PM (1 year, 3 months ago) |
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Quote:
Rusty2096 said:
Quote:
CJD said: If I'm being honest... I'm waiting for semp pics mostly. I keep checking back on that thread hoping to see progress pictures. But I'll settle for water agar tests if they come first. 
Had some unprotected sex lately. Pretty sure the baby will come fist 
I thought we weren't gonna tell anyone yet 🙊
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rumfor69
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Re: Let's talk about Water Agar!! 💧 [Re: Rotnpins] 2
#27994246 - 10/11/22 07:04 PM (1 year, 3 months ago) |
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Quote:
Rotnpins said:
Quote:
rumfor69 said:
Quote:
Rusty2096 said:
Quote:
CJD said: If I'm being honest... I'm waiting for semp pics mostly. I keep checking back on that thread hoping to see progress pictures. But I'll settle for water agar tests if they come first. 
Had some unprotected sex lately. Pretty sure the baby will come fist 
I thought we weren't gonna tell anyone yet 🙊
Awww... little Rusty-Rum babies?
Rum, why you been cheating on me? Don't I keep you satisfied?
Don't worry Rotn, you'll always be my bottom bitch 😘
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rumfor69
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Re: Let's talk about Water Agar!! 💧 [Re: Screwup] 1
#28021097 - 10/28/22 10:48 AM (1 year, 2 months ago) |
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Quote:
Screwup said:
Quote:
hazyhorse said: gonna start spitting on my plates just to have a challenge again
From which orifice on your body?
All of them, gotta have consistency in experimentation
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rumfor69
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Re: Let's talk about Water Agar!! 💧 [Re: Rotnpins]
#28021535 - 10/28/22 02:31 PM (1 year, 2 months ago) |
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rumfor69
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Re: Let's talk about Water Agar!! 💧 [Re: Rotnpins]
#28021549 - 10/28/22 02:35 PM (1 year, 2 months ago) |
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I definitely am. The min i get a questionable dish or take a new clone I'm going right to WA immediately with it lol
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rumfor69
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Re: Let's talk about Water Agar!! 💧 [Re: Rotnpins]
#28025729 - 10/31/22 08:22 AM (1 year, 2 months ago) |
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That's interesting stuff. Nutrient agar transferred to a water slant grows out some then stops when it runs outta food but stays viable for a yr or two
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rumfor69
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Re: Let's talk about Water Agar!! 💧 [Re: Land Trout]
#28080269 - 12/02/22 07:53 PM (1 year, 1 month ago) |
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I think WA is just good for getting away from bacteria. Molds are still gonna grow on it.
Outdoor clone I'd say goto nutritional plate first, isolate the mycelium you want then goto WA to get away from bacteria, then back to nutritional plate.
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rumfor69
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Re: Let's talk about Water Agar!! 💧 [Re: Seeker2be]
#28160570 - 01/27/23 10:06 PM (1 year, 6 hours ago) |
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rumfor69
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Re: Let's talk about Water Agar!! 💧 [Re: Shrimps] 2
#28164266 - 01/30/23 11:56 AM (11 months, 21 days ago) |
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I've thought about his absence also. Hopefully he's good.
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rumfor69
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Re: Let's talk about Water Agar!! 💧 [Re: SeaSalt]
#28224204 - 03/10/23 06:12 PM (10 months, 13 days ago) |
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I'm not sure. But the theory here is that the bacteria doesn't grow out from the transferred piece. So if you grab some myce that has grown out towards the edge your going to get something clean. I suggest transferring while back lighting in a dark room under magnification if possible.
I miss Rotn
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rumfor69
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Re: Let's talk about Water Agar!! 💧 [Re: herbstation]
#28233540 - 03/17/23 10:53 AM (10 months, 6 days ago) |
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Usually water agar is a tool used when you have a bacterial infected culture. While you can probably germinate spores on it. I would personally germinate on regular agar first.
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rumfor69
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Re: Let's talk about Water Agar!! 💧 [Re: herbstation]
#28233629 - 03/17/23 12:02 PM (10 months, 6 days ago) |
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Just remember this tek wont help with mold contamination cuz it's mycelium and will grow on WA. This tool is only for bacteria.
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rumfor69
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Re: Let's talk about Water Agar!! 💧 [Re: SeaSalt]
#28233791 - 03/17/23 01:30 PM (10 months, 6 days ago) |
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It's probably gonna look real wispy and difficult if not impossible to distinguish from the mycelium you want. If you think a culture has mold, regular agar would be better at feeding it so it shows stronger characteristics that allow you to transfer away from it easier. Just my opinion.
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