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InvisibleUnknownfungi
Amateur mycologist
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Registered: 09/28/21
Posts: 584
Panaeolus cinctulus Miraculix potency test * 7
    #27884555 - 07/31/22 08:02 PM (1 year, 5 months ago)

Round number three is live and onstage is Panaeolus cinctulus in this episode of

How Strong is That


The cinctulus was collected a month ago and dried immediately at 155f. Record of the cinctulus find is here

150mg of the sample was ground into a fine powder. Miraculix instructions were followed just as in other testing episodes.

A darkening color change occured indicating psilocybin. Yet this color change did not fit well with the provided color chart and is hard to pick up on camera. The color changed to a dark brown with a greenish tint. I had a peer check the sample and both of us couldn't place it well on the color chart due to the green tint.

We both agreed the colors best fit ranged between 1.2%-1.5%  or 12mg-15mg of psilocybin per gram of dried material. I would say on the higher end of that range. That is spicy!

Personally I'm not happy with these results and might consider talking with Miraculix and/or retesting yet I would think the same color change would reoccur.

I'd be interested to hear what others think when comparing the sample to the color chart. How strong does it look to you?







 


Now what happened to cause the green color change? I don't have an answer but my best guess is interference with similar reacting chemicals contained in Panaeolus cinctulus.


I hope you all enjoyed this episode of How Strong is That?


--------------------
Gymnopilus luteofolius potency results
Gymnopilus luteus potency results
Active Pluteus potency results
Panaeolus cinctulus potency results
Psilocybe caerulipes potency results
Gymnopilus luteus
Gymnopilus luteofolius
Psilocybe ovoideocystidiata
Psilocybe caerulipes
Panaeolus cinctulus
Pluteus americanus


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OfflineMentalPariah
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Re: Panaeolus cinctulus Miraculix potency test [Re: Unknownfungi]
    #27884667 - 07/31/22 09:14 PM (1 year, 5 months ago)

That seems a wee bit stout for cinctulus. I would almost wager to bet it's reacting also with other indole like molecules, mainly serotonin and 5htp which are also insoles closely resembling psilocybin/psilocin


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Whoever appeals to the law against his
Fellow man is either a fool or a coward
Whoever cannot take care of himself without that law is both
For a wounded man shall say to his assailant
If I live I will kill you, if I die you are forgiven
Such is the rule of honor


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OfflineDave Bowman
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Re: Panaeolus cinctulus Miraculix potency test [Re: Unknownfungi]
    #27884671 - 07/31/22 09:16 PM (1 year, 5 months ago)

Cool test and cool test kit :mushroom2:


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OfflineAnglerfishM
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Re: Panaeolus cinctulus Miraculix potency test [Re: MentalPariah] * 1
    #27884876 - 08/01/22 03:06 AM (1 year, 5 months ago)

Quote:

MentalPariah said:
That seems a wee bit stout for cinctulus. I would almost wager to bet it's reacting also with other indole like molecules, mainly serotonin and 5htp which are also insoles closely resembling psilocybin/psilocin




Maybe it's my eyes, but to me the solution in the glass seems to correspond more with 6 mg .


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Offlinewojownik
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Re: Panaeolus cinctulus Miraculix potency test [Re: Anglerfish]
    #27884879 - 08/01/22 03:19 AM (1 year, 5 months ago)

I agree, but also, my eyes aren't the best.


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OfflineAnglerfishM
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Re: Panaeolus cinctulus Miraculix potency test [Re: wojownik]
    #27884889 - 08/01/22 03:40 AM (1 year, 5 months ago)

Comparing to the result for Pluteus salicinus in this thread, which landed on
the same potency range, the colour is very different to my eyes, more vividly orange-brown and compares
much better to the chart. The result for P. cinctulus appears much duller and the solution looks almost dirty.

My question is - how fine is the filtering process? Is there a chance that spores from the samples might pass into the final liquid?


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Offlineknarkkorven
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Re: Panaeolus cinctulus Miraculix potency test [Re: Anglerfish]
    #27887059 - 08/02/22 03:09 PM (1 year, 5 months ago)

I'm also thinking there could be cross reactions to the similar molecules present in P. cinctulus.

I think this test should more be used to test "is it active/weak/strong?", not "exactly how strong is it?".

This phenotype of P. cinctulus is often dosed as P. cubensis in weight, so most probably the true potency is well below 1%.


But great to see this and the Pluteus test/thread as well! :cheers:


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Offlinethe man
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Re: Panaeolus cinctulus Miraculix potency test [Re: knarkkorven]
    #27887626 - 08/03/22 03:00 AM (1 year, 5 months ago)

while0.6% makes the most sense. its pretty clearly more brown than it is orange or yellow. infact see very little orange if any att all putting it higher on the %. i dont know limitations of the test but perhaps as others have said cross reactive to other tryptamine alkaloids?  was weighed correctly? as sample size being accurate is very important in these types of assays, well most assays lol.. prob need 3 poin scale as could be almost 10% extra? ie could be close to 0.17 pretty easily.

also using caps with dark spores may muddy things... if was 1.6% prob would be closer to pan cyan potency, which is probably incorrect.


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InvisibleUnknownfungi
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Registered: 09/28/21
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Re: Panaeolus cinctulus Miraculix potency test [Re: the man] * 2
    #27891668 - 08/05/22 07:04 PM (1 year, 5 months ago)

Sorry a bit late but let my try and answer some of the questions.


Filtering is done via a Sterifilt membrane filter. A basic Sterifilt "eliminates over 90% of all insoluble particles of over 5 µm and over 93% of all particles larger than 10 µm".
In original post, fourth picture down you can see the 1ml of filtered solution in the syringe. The solution was clear with no solids seen by the eye. Therefore I don't believe any non-soluble matter made a difference in the results. Where the Gymnopilus luteus test visibly changed the color of the extract with its soluble brightly colored yellow and orange pigments but still worked fine.
The amount of extract that is added is minimal compared to the volume of testing liquid.

The sample was weighed multiple times but you do bring up a great point in that a more accurate scale such as a 0.001 scale would be more appropriate for small samples. The error with the scale used is +-10mg. That's equal to +-6.67% when considering the sample is 150mg.

Side reactions with indole ring byproducts such as serotonin. All Panaeolus contain serotonin to varying degrees. Other substances such as 5-htp can also be found.  This is where I place my bet for the wonky color change. I'd love to see some other active and inactive Panaeolus species tested for comparison.


Side notes- It cannot be seen in the pictures but the sample had quite the apparent green/yellow tint. The sample was the darkest in color out of all the tests I've done but never fit with the chart because it's color.


--------------------
Gymnopilus luteofolius potency results
Gymnopilus luteus potency results
Active Pluteus potency results
Panaeolus cinctulus potency results
Psilocybe caerulipes potency results
Gymnopilus luteus
Gymnopilus luteofolius
Psilocybe ovoideocystidiata
Psilocybe caerulipes
Panaeolus cinctulus
Pluteus americanus


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OfflineShroomz420
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Re: Panaeolus cinctulus Miraculix potency test [Re: Unknownfungi]
    #27891921 - 08/05/22 10:24 PM (1 year, 5 months ago)

I just love the way pan cincts look especially the stems. Nice post!


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InvisibleHartford
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Re: Panaeolus cinctulus Miraculix potency test [Re: Shroomz420]
    #27892042 - 08/06/22 02:32 AM (1 year, 5 months ago)

Looks like 18 mg (1,8%) to me.


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InvisibleIcyurmt
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Re: Panaeolus cinctulus Miraculix potency test [Re: Unknownfungi]
    #27892623 - 08/06/22 02:44 PM (1 year, 5 months ago)

:congrats: I'm a bit late to the party, but hay! Thank You for doing and sharing these tests!!

I agree with everyone, based on the color there is likely other side reactions skewing it to look higher. I guessed they would turn out similar to normal cubes based on my own experiences with them. Have you sent these results to the company to see what they can say about it?


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👁️ 🌊 why you are empty.

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OfflineAdas
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Re: Panaeolus cinctulus Miraculix potency test [Re: Anglerfish]
    #27951478 - 09/15/22 02:04 PM (1 year, 4 months ago)

Quote:

Anglerfish said:
Quote:

MentalPariah said:
That seems a wee bit stout for cinctulus. I would almost wager to bet it's reacting also with other indole like molecules, mainly serotonin and 5htp which are also insoles closely resembling psilocybin/psilocin




Maybe it's my eyes, but to me the solution in the glass seems to correspond more with 6 mg .




Same, I'd also place it in the 6mg ballpark.


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