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Invisiblejonneill
Hero
Registered: 06/08/04
Posts: 133
Question about flame sterilization
    #2786871 - 06/12/04 02:31 AM (13 years, 5 months ago)

I have read that petrochemicals such as butane or pentane, even in small amounts, can be harmful to mycelium, and that even residue from burning them can adversely affect the fungus. I was planning on making my own spore syringes but I don't have an alchohol lamp with which to sterilize the needle... I figure it's a good idea to flame-sterilize them between innoculating jars, maybe after every 3 or 4, just to be on the safe side.

The question: has anyone noticed anything bad stemming from use of a "dirty" flame like a lighter or a smallish blowtorch as opposed to a clean alchohol flame?


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OfflineSev
Astropath
Registered: 06/06/03
Posts: 1,426
Loc: NY
Last seen: 3 years, 1 month
Re: Question about flame sterilization [Re: jonneill]
    #2786890 - 06/12/04 02:40 AM (13 years, 5 months ago)

Naw.


--------------------
"Do we want the stars? We can have them. Can we borrow cups of fire from the sun? We can and must and light the world." --"On the Shoulders of Giants", Ray Bradbury

All of my posts are full of fiction and blatant lies.


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OfflinePsilocybeingzz
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Registered: 12/15/02
Posts: 14,463
Loc: International waters
Last seen: 5 years, 17 days
Re: Question about flame sterilization [Re: jonneill]
    #2786923 - 06/12/04 02:55 AM (13 years, 5 months ago)

Rub alcohol on the needle, flame it inject, rub alcohol on the needle ,flame it , cap it.

SIMPLE.

and if the pertrochemicals hurt mycelium(what almost unthinkable ammountof residue  thats there) well the mycelium I have seen has never heard of that. :smile:

Petrochemical are destroying the planet, not your shroom jars. Dont worry...about your jars anyway. :smile:


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Offlinetrippysmurf
Smurfette'sSugar Daddy

Registered: 02/22/04
Posts: 195
Last seen: 11 years, 9 months
Re: Question about flame sterilization [Re: Psilocybeingzz]
    #2787471 - 06/12/04 12:31 PM (13 years, 5 months ago)

hm... Never flamed. Just used alcohol. Never a contam.


--------------------
I like your Christ, but I do not like Christians - they are so unlike Christ. -Gandhi


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Offlinesublimesubmind
enthusiast
Registered: 05/25/04
Posts: 264
Last seen: 11 years, 4 months
Re: Question about flame sterilization [Re: trippysmurf]
    #2787506 - 06/12/04 12:46 PM (13 years, 5 months ago)

i didnt even sterilize between my 12 cakes, and i didnt get a single contam. So i bet a alcohol swab is more than enough, flaming is just being paranoid


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Offlinedeath_by_desire
me
Registered: 05/26/04
Posts: 99
Loc: In your mind!
Last seen: 13 years, 2 months
Re: Question about flame sterilization [Re: sublimesubmind]
    #2787583 - 06/12/04 01:11 PM (13 years, 5 months ago)

What about using disinfectant spray instead of alcohol? Does that work to?!?!


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OfflineDr. Greenthumb
HipHopHead 4Life

Registered: 12/15/99
Posts: 333
Last seen: 10 months, 2 days
Re: Question about flame sterilization [Re: death_by_desire]
    #2787604 - 06/12/04 01:24 PM (13 years, 5 months ago)

Quote:

death_by_desire said:
What about using disinfectant spray instead of alcohol? Does that work to?!?!



Why stray from the tried and true method ecspecially when a bottle of alcohol costs under a buck?


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Offlinesublimesubmind
enthusiast
Registered: 05/25/04
Posts: 264
Last seen: 11 years, 4 months
Re: Question about flame sterilization [Re: Dr. Greenthumb]
    #2787652 - 06/12/04 01:52 PM (13 years, 5 months ago)

yeah, any walgreens has it, its so cheap it they probably wouldnt care if you stole it:)


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