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Invisiblebakedbeings
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Registered: 09/01/20
Posts: 4,563
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Re: reviving +15 year old spores [Re: SymPlayTon]
    #28001525 - 10/16/22 08:46 AM (2 years, 2 months ago)

OP is playing mush cult on hard mode


--------------------
Confused? Well now you can!
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OfflineSymPlayTon
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Registered: 09/03/22
Posts: 420 420 Posts!
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Re: reviving +15 year old spores [Re: bakedbeings]
    #28001535 - 10/16/22 08:57 AM (2 years, 2 months ago)

He is just using it as a flex so other newbs like me can't complain. 

:pointmade:

Edited by SymPlayTon (10/16/22 08:57 AM)

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Offlinegoldcap
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Re: reviving +15 year old spores [Re: Rusty2096]
    #28004547 - 10/18/22 11:24 AM (2 years, 2 months ago)

Quote:

Rusty2096 said:
Quote:

goldcap said:
I wonder how many newbie-no-nothin-wanna-be-growers (like me) find themselves having the problem of reviving decades old spores... can not possibly be that many.




1 - you

The other noobs buy grow kits :lol:


Quote:

21 Celsius seems to much close to ideal growing conditions



21 is perfect. Higher temps you were maintening (like 26) is still inside cubes myc acceptable range but the thing is, at that temp, you will favorise bacterial growth.

Spores can take up to many weeks to germinate (especially older ones). While moving to 21c was the right thing to do , I doubt that was the turning point for germination. More likely a coincidence.




I dont believe in coincidences, so the most likely reason is that on the same day I noticed growth,I also shaved my legs and drop my computer on the ground... yeaaa. that have to be it!

But hey, yea probably I was just damn lucky. Good point about keeping temperature lower to reduce contaminates! I will keep it lower!

I did lie a little bit though... I did grow some shrooms like 15 years ago so its not the first time ever. I ate them maybe 2 times and made 30 prints or something and then never thought of it again.

Out of these 30 prints, I have gotten 2 previous ones to develop into clouds. But since I am well basically a n00b for all intents and purposes, I used the most horrible growing techniques and contaminants had my cubes for breakfast.

Now I got no more prints but one jar with a cloud, lol. Lucky bastard right! :sun:

My plan is to suck up the myc cloud from the LC and drop it onto several agar plates.

Then if the myc grows I will cut out healthy agar and move onto other agar plates, so they I get plenty of plates.

Then I guess I will wait for one of the agars to be fully overgrown by myc, before attempting to inoculate a jar with grains.

I am aiming for getting from myc to mushroom ASAP... so I get can some fresh spores....

...then I will wait 30 years and see if I am able to germinate those :eek::tongue2:

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Offlinegoldcap
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Re: reviving +15 year old spores [Re: SymPlayTon]
    #28004563 - 10/18/22 11:34 AM (2 years, 2 months ago)

Quote:

Bakedbeings said:
OP is playing mush cult on hard mode




Quote:

SymPlayTon said:
He is just using it as a flex so other newbs like me can't complain. 

:pointmade:




Well I lied a little. I did make the spores myself all those years back. I did a handful of grows and made some spores. Not super n00b. But clearly I am at least making n00b mistakes here, so well... :blush:

I made spore prints sandwiched between glass and taped up so no air could get inside. Stored in ziplock. Darkness. Room temperature. Perhaps 2 of those prints produces clouds in LC.

This last print was made on tinfoil, the edges of the print folded so no air could get inside. Stored in ziplock bag. Room temperature.

Apparently tinfoil worked basically as well as glass, for preservation. But they can easily get damaged by accident, tearing a whole.

N00b or not, I hope this thread will be helpful for other people.

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OfflineSymPlayTon
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Re: reviving +15 year old spores [Re: goldcap]
    #28005649 - 10/18/22 11:24 PM (2 years, 2 months ago)

I am excited to keep up with your results and see your grows! 

I hope to learn a lot from your logs.

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Offlinegoldcap
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Re: reviving +15 year old spores [Re: SymPlayTon] * 1
    #28006178 - 10/19/22 10:06 AM (2 years, 2 months ago)

Quote:

SymPlayTon said:
I am excited to keep up with your results and see your grows! 

I hope to learn a lot from your logs.




Smart move! If you follow me closely I can make all the mistakes so you dont have to :grin:

I will keep the thread updated of my results.

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Offlinegoldcap
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Re: reviving +15 year old spores [Re: goldcap]
    #28006241 - 10/19/22 10:46 AM (2 years, 2 months ago)

How to sterilize syringe?

I tried boiling it (PC), but it damaged the syringe (the plastic went bad and water leaked out when pressing the plunger).

I dont have a UV lamp strong enough to kill bacteria...

But I am thinking that maybe I could suck up some chlorine, leave it in the syringe a little while and then empty the syringe. If I wait like 7 days the chlorine drops still remaining should have evaporated?!

Are there any better tricks?

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Invisiblebakedbeings
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Re: reviving +15 year old spores [Re: goldcap]
    #28006256 - 10/19/22 10:51 AM (2 years, 2 months ago)

not sure what went wrong but i sterilize syringes in the PC all the time. i reuse the same ones for years and they still work. they are the originals from when i bought spore syringes

ive heard of people simply boiling water and pulling it into the syringe, pushing it out, and repeating several times. gets the thing pretty clean i guess. but i dont see why you cant do it in the PC


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Offlinegoldcap
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Re: reviving +15 year old spores [Re: bakedbeings]
    #28007309 - 10/20/22 01:18 AM (2 years, 2 months ago)

Quote:

bakedbeings said:
not sure what went wrong but i sterilize syringes in the PC all the time. i reuse the same ones for years and they still work. they are the originals from when i bought spore syringes

ive heard of people simply boiling water and pulling it into the syringe, pushing it out, and repeating several times. gets the thing pretty clean i guess. but i dont see why you cant do it in the PC




Thanks for your input and this confirms what I have read elsewhere.

My first idea was to PC the syringe and I tried it, then tested it and noticed that it stopped working.

My bet is that your syringe has an O-ring at the bottom of the plunger, that is slightly bigger then the inside of the syringe, so that the O-ring is pressed against the sides. That way no water can leak out when you apply pressure on the plunger.

My syringe has no O-ring. Instead it is relying on having like a perfect fit to the inside of the syringe. I noticed this at once when I got the syringe and was confounded "will this really work?".

Actually it works really well. I can even put rubberbands (forced hydration) on the plunger, which will slightly bend the top of the plunger (where you put would out finger when pressing) but no air or water is able to leak out, between the plunger and the inside how the syringe.

After having PC the syringe, its no longer air or water tight. My bet is that the high temperature deformed the plastic (I dont believe in magic and dont think nano technology played any part in this particular case.)... has got the be the heat.

So therefor I got 2 ways to go with sterilizing without heat:
UV-light
chemicals

UV-light is nasty stuff... can easily damage your eyes and stuff. I dont want that crap lying around in my tent, should a cat or some of the nature people wander around and find it and look into it by accident.

Chemicals: I got isopropanol and chlorine. Both are easily obtainable. Isopropanol can be found in most disinfectant. Even hand disinfectants could maaaaybe work. They usually have some glycerine, but that actually only a benefit because it will make it less likely for the spores to attack to the syringe itself. But there is other crap in it as well, such as some kind of thickeners chemicals and I dont know how that will affect the spores. Then there are car cleaning stuff that contains isopropanol, but who knows what other kinds of chemical residues might be there... I mean its not food graded ;-)

However, I already got some chlorine at hand and hey, accordingly to internet it breaks down in a few days (not as fast as isopropanol though). Especially if the sun (UV) is shining on it. Less of a fire hazard too...

So rinsing the syringe with water, then sucking up some chlorine and then putting it in the sun to dry out... that should work. Chlorine is not food graded either, there will be some toxic stuff left from that too.

Hmm... maybe rinsing out the syringe with chlorine then cleaning it out with pre-boilied water would work. That way there would be no chemicals left. Yea thats probably it.

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Offlinegoldcap
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Re: reviving +15 year old spores [Re: goldcap]
    #28025559 - 10/31/22 03:00 AM (2 years, 2 months ago)

Update:
All the agar plates have a really healthy and thriving green mold looking ugly stuff growing on them. It looks like the cloud I thought was cubensis, was anything but. That was my last spore print, so end of this test. Fun as long as it lasted.

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