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OfflineSirPsycho
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I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. * 2
    #27853904 - 07/07/22 06:24 PM (1 year, 6 months ago)

I've using a little tool to cut perfect 1/4" circles of agar for about a year now. Kinda ghetto but very good functionality. It was a little paint brush with the bristles pulled out and a hole in the side of the metal sleeve so it doesn't suction up the transfer pieces and very thin guage so it doesn't stay hot long and melt my reusable PP5 petris.
:rollsafe:

Here's the problem though, it forces you to do one of two things in the SAB.

1. Place your hand directly over the open (big no no) plate since the tool needs to come down perpendicular to the surface to cut the little circle.

2. Remove the lid of the plate completely (another big no no) and put it aside to pick up the plate at an angle. I never actually did this one

Coincidentally... :facepalm3: I also happened to notice an increase in bacterial contam on my plates in that time period but just never made a connection.

So yeah, until I get a LFH and can "safely" open containers fully, I'm gonna stick with a knife and just slightly opening plates.


--------------------
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OfflineSockadin
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: SirPsycho] * 2
    #27853914 - 07/07/22 06:31 PM (1 year, 6 months ago)



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OfflineSirPsycho
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: Sockadin]
    #27853915 - 07/07/22 06:34 PM (1 year, 6 months ago)

I like it, I use a loop to cut bits of agar out of 15ml slant tubes. I can get a bit thicker guage wire and bend it up all how I need. You're a gentleman and a scholar fine sir, thank you


--------------------
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Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

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OfflineSockadin
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: SirPsycho] * 1
    #27853920 - 07/07/22 06:37 PM (1 year, 6 months ago)

Gotta be honest I haven't had to flame sterilize in a year and have very few issues other than drunken transfers that occasionally get ditched. And thank you for your compliment. I do enjoy your posts.


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OfflineSirPsycho
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: Sockadin]
    #27853927 - 07/07/22 06:40 PM (1 year, 6 months ago)

Oh I plan on sticking it on the end of an x-acto handle and flaming the hell out of it.

Also, thank you as well.


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

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InvisibleGuerrilla
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: SirPsycho] * 2
    #27853938 - 07/07/22 06:49 PM (1 year, 6 months ago)

My friend, these metal straws work great and completely avoid the issues mentioned in your post.



--------------------
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Edited by Guerrilla (07/07/22 06:51 PM)


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InvisibleElf_on_a_Log
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: SirPsycho] * 1
    #27853960 - 07/07/22 07:09 PM (1 year, 6 months ago)

Quote:

SirPsycho said:
1. Place your hand directly over the open (big no no) plate since the tool needs to come down perpendicular to the surface to cut the little circle.



Have you tried holding the transferring plate sideways or top-angled-down?  The receiving plate can't be at a steep angle for obvious reasons but doing that with the transferring plate greatly cuts contam chances.

That no-flame paperclip method is interesting.  Going to have to give that a try sometime.


--------------------
"I was strolling through the woods one day when I came upon an elf sitting on a log.  He offered me a strange-looking mushroom to eat.  As soon as I ate it, I realized that I was the elf on the log and that my human life had been but a dream."
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OfflineSirPsycho
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: Guerrilla]
    #27853980 - 07/07/22 07:39 PM (1 year, 6 months ago)

Quote:

Guerrilla said:
My friend, these metal straws work great and completely avoid the issues mentioned in your post.





I've looked at those a few times, they look like a lot of metal. Do they stay hot a long time?


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
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  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023


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OfflineSirPsycho
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: Elf_on_a_Log]
    #27853981 - 07/07/22 07:41 PM (1 year, 6 months ago)

Quote:

Elf_on_a_Log said:
Quote:

SirPsycho said:
1. Place your hand directly over the open (big no no) plate since the tool needs to come down perpendicular to the surface to cut the little circle.



Have you tried holding the transferring plate sideways or top-angled-down?  The receiving plate can't be at a steep angle for obvious reasons but doing that with the transferring plate greatly cuts contam chances.

That no-flame paperclip method is interesting.  Going to have to give that a try sometime.



I have, it's a bit un-widely for my liking while holding onto the lid with the same hand.


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023


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OfflineCocaineBuffet
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: SirPsycho] * 5
    #27854098 - 07/07/22 09:42 PM (1 year, 6 months ago)

I just cut squares or triangles out of the donating plate to transfer. I think there are some benefits to using a punch but there are too many vectors to make me feel like I need to switch.

Nice uniform plates are dope don't get me wrong but they are hardly necessary.


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Offline99.99
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: SirPsycho] * 1
    #27854112 - 07/07/22 09:59 PM (1 year, 6 months ago)

I use clay sculpture tools…. you can wrap them up in foil and sterilize them the same time you’re doing your agar



Amazon and eBay has these for a couple bucks a set


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InvisibleThe Fresh PrintsS
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: 99.99] * 1
    #27854146 - 07/07/22 10:48 PM (1 year, 6 months ago)

Definitely glad you made the distinction for SAB work. Punch tools are great in front of the hood, but the SAB is a whole different ball game


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OfflineSirPsycho
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: CocaineBuffet]
    #27854307 - 07/08/22 04:55 AM (1 year, 6 months ago)

Quote:

99.99 said:
I use clay sculpture tools…. you can wrap them up in foil and sterilize them the same time you’re doing your agar



Amazon and eBay has these for a couple bucks a set



When I use the inoculation loop to grab agar out of slants it's used in a similar fashion to those tools. I'll cut away ribbons of agar by dragging the loop across the surface

Quote:

The Fresh Prints said:
Definitely glad you made the distinction for SAB work. Punch tools are great in front of the hood, but the SAB is a whole different ball game



They are ok in the SAB too just not really best practice. It did increase my contam rate but just slightly, hence taking a whole damn year to put 2 and 2 together.

Quote:

CocaineBuffet said:
I just cut squares or triangles out of the donating plate to transfer. I think there are some benefits to using a punch but there are too many vectors to make me feel like I need to switch.

Nice uniform plates are dope don't get me wrong but they are hardly necessary.




Beyond pretty plates, I also found it really handy when doing lots of A2G. Only been doing A2G into pints of millet lately though, I then use the millet to noc other stuff. So I've kinda lost out on the A2G benefit of it too.

Pretty much just left with another couple of vectors and some not so best practice.

Gonna give the paper clip loop a shot, seems to solve all my handling issues and I'm not worried about melting through plates or burning my hands, which are the reasons I've avoided the straws Guerilla posted.


--------------------
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Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023


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OfflineDERRAYLD
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: SirPsycho] * 2
    #27854309 - 07/08/22 04:56 AM (1 year, 6 months ago)

I use punches quite rarely as well, the circle looks good but the growth is always very similar to a triangle without the added vector.


Edited by DERRAYLD (07/08/22 06:38 AM)


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InvisibleLotKid
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: DERRAYLD] * 4
    #27854344 - 07/08/22 06:08 AM (1 year, 6 months ago)

#7 scalpel handle and #11 blades for the win.

I've never found the hole punch method appealing.


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OfflineSirPsycho
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: LotKid]
    #27854377 - 07/08/22 06:47 AM (1 year, 6 months ago)

Well damn, Jackie!

I just made my way through the 1000 x-acto blades I got when I started and ordered a proper scalpel yesterday but I went with a #3 handle


--------------------
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Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: SirPsycho] * 3
    #27854457 - 07/08/22 08:18 AM (1 year, 6 months ago)

get yourself a #7 handle. it has the longest reach. and #11 blades have a nice point on them for spearing your transfers.


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OfflineSpirit-Crusher
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: LotKid]
    #27854490 - 07/08/22 08:38 AM (1 year, 6 months ago)

Quote:

LotKid said:
get yourself a #7 handle. it has the longest reach. and #11 blades have a nice point on them for spearing your transfers.



This is what I use as well.

I got caught up in trying to use a punch for like a minute. My agar always got stuck in the punch hole. Then I realized it was a waste of time for me. I just use squares and my plates look just fine, but even more important, they're functional.


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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. *DELETED* [Re: SirPsycho]
    #27854493 - 07/08/22 08:40 AM (1 year, 6 months ago)

Post deleted by SharaVabdas

Reason for deletion: Shroomery is full of bigots and I'm leaving.


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OfflineSirPsycho
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: SharaVabdas]
    #27854521 - 07/08/22 09:05 AM (1 year, 6 months ago)

Quote:

LotKid said:
get yourself a #7 handle. it has the longest reach. and #11 blades have a nice point on them for spearing your transfers.



Yeah, I will and those are the blades I got

Quote:

SharaVabdas said:

You're not really supposed to cut to the bottom of the agar, you're supposed to just grab a thin, top layer to minimize contact with uncolonized agar from the source plate.




Never heard that before.

I know it's best practice to transfer while the myc is still 1/4" away from the edge of the plate and you're not supposed to transfer that uncolonized agar since the edges of the plate are where contam is most likely.


--------------------
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Balance in life is like running on ice.

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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: SirPsycho] * 4
    #27854551 - 07/08/22 09:38 AM (1 year, 6 months ago)

I've never heard that either. Mainly because i think its bullshit.


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OfflineDERRAYLD
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: LotKid] * 1
    #27854585 - 07/08/22 10:18 AM (1 year, 6 months ago)

:lol:


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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: DERRAYLD]
    #27854646 - 07/08/22 11:13 AM (1 year, 6 months ago)

:popcorn::takingnotes:


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OfflineSirPsycho
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: LotKid]
    #27854657 - 07/08/22 11:18 AM (1 year, 6 months ago)

Quote:

LotKid said:
I've never heard that either. Mainly because i think its bullshit.



What I said about transferring before the myc reaches the edge of the plate or what they said?


--------------------
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Balance in life is like running on ice.

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"Mist your balls and fan your asshole" - Pandaskis, 2023


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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. *DELETED* [Re: SirPsycho] * 1
    #27854720 - 07/08/22 12:17 PM (1 year, 6 months ago)

Post deleted by SharaVabdas

Reason for deletion: Shroomery is full of bigots and I'm leaving.


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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: SharaVabdas] * 2
    #27854739 - 07/08/22 12:27 PM (1 year, 6 months ago)

If the plate is clean neither matter. I do both all the time.

But specifically, i've never heard the "dont cut all the way to the bottom of the plate". I dont think thats a real thing. Maybe it is among the science scholars of reddit, that could be true. :shrug:


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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: LotKid]
    #27854928 - 07/08/22 02:27 PM (1 year, 6 months ago)

I mostly work with my plates face down in the SAB and pick the filled side up and work with it at and angle such as the open side is facing somewhat downwards then it's always above my tools. Sure, contaminants may fall in the lid side, but it works pretty reliably for me. Sometimes such as when you're lifting your transfer out you will need to turn the open face upwards a bit so gravity is helping you control your transfer but I never work with the open plate on the 'floor'.


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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: Bobbins]
    #27854969 - 07/08/22 03:03 PM (1 year, 6 months ago)

that sounds like a pain in the ass.


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OfflineSirPsycho
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: LotKid]
    #27855009 - 07/08/22 03:48 PM (1 year, 6 months ago)

Quote:

LotKid said:
If the plate is clean neither matter. I do both all the time.

But specifically, i've never heard the "dont cut all the way to the bottom of the plate". I dont think thats a real thing. Maybe it is among the science scholars of reddit, that could be true. :shrug:



Yeah, I'm not really concerned about the edge of the plate either. Just remember reading it in a Stamets book and figured they might be getting their thing confused for the Stamets thing


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023


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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: SirPsycho]
    #27855123 - 07/08/22 05:39 PM (1 year, 6 months ago)

I never bothered to read stamets books. :shrug:


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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: LotKid]
    #27855145 - 07/08/22 06:08 PM (1 year, 6 months ago)

:whathesaid:

Also mycelium grows outward in all directions so not grabbing the bottom of the plate does absolutely nothing.


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OfflineSirPsycho
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: Sockadin]
    #27855219 - 07/08/22 07:29 PM (1 year, 6 months ago)

Lmfao, y'all aren't missing out on much. The books are like 40 and 20 years old


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: SirPsycho]
    #27855387 - 07/08/22 09:40 PM (1 year, 6 months ago)

I can't hold a blade steady enough to cut and pick up a small wedge. I placed a sharpened 8 g transfer needle on a syringe and shaped a bicycle some as a plunger to push out the plug. It's 3.4 mm I.D.  now that I think about it I've had more contams than I'd like to see. I previously tried an innoc loop but it flexed too much. I'm going to try the clay sculpture tools.


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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: SirPsycho] * 1
    #27855650 - 07/09/22 06:46 AM (1 year, 6 months ago)

Quote:

SirPsycho said:
Quote:

Guerrilla said:
My friend, these metal straws work great and completely avoid the issues mentioned in your post.





I've looked at those a few times, they look like a lot of metal. Do they stay hot a long time?




No, less than a scalpel. Never gets too hot to handle, heat doesn't transfer up the straw even in long transfer sessions.


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OfflineBrian Jones
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. *DELETED* [Re: SharaVabdas]
    #27855657 - 07/09/22 06:54 AM (1 year, 6 months ago)

Quote:

SharaVabdas said:
Post deleted by SharaVabdas

Reason for deletion: Shroomery is full of bigots and I'm leaving.




I never noticed anything like that in the cultivation forum.


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InvisibleGuerrilla
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. *DELETED* [Re: Brian Jones] * 1
    #27855661 - 07/09/22 07:00 AM (1 year, 6 months ago)

Quote:

Brian Jones said:
Quote:

SharaVabdas said:
Post deleted by SharaVabdas

Reason for deletion: Shroomery is full of bigots and I'm leaving.




I never noticed anything like that in the cultivation forum.




He's deleted all 489 of his posts with the same comment! Whoa


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InvisibleLotKid
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. *DELETED* [Re: Guerrilla] * 4
    #27855673 - 07/09/22 07:13 AM (1 year, 6 months ago)

I got curious and looked up his/hers/their/its posts. And they were posting in the pub on a trans woman thread and got offended by looks of it. I dont see what was so offensive but appearently sex and gender was more important to them then every other aspect of this site.

I mean, it was in the pub, where its okay to offend, what did they expect?


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InvisibleInfinite Monkeys

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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. *DELETED* [Re: SirPsycho]
    #27855711 - 07/09/22 07:52 AM (1 year, 6 months ago)

Is there a method to make cuts without "ripping" or "dragging" rhizo growth off of the top of agar plates?

One reason I like the punch method is because the myc doesn't seem to rip.


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OfflineSirPsycho
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. *DELETED* [Re: LotKid]
    #27855716 - 07/09/22 07:58 AM (1 year, 6 months ago)

Quote:

Guerrilla said:
Quote:

SirPsycho said:
Quote:

Guerrilla said:
My friend, these metal straws work great and completely avoid the issues mentioned in your post.





I've looked at those a few times, they look like a lot of metal. Do they stay hot a long time?




No, less than a scalpel. Never gets too hot to handle, heat doesn't transfer up the straw even in long transfer sessions.



I'll grab a set and give them a go as well.
Quote:

LotKid said:
I got curious and looked up his/hers/their/its posts. And they were posting in the pub on a trans woman thread and got offended by looks of it. I dont see what was so offensive but appearently sex and gender was more important to them then every other aspect of this site.

I mean, it was in the pub, where its okay to offend, what did they expect?



Yep, I did the same. Parts of their posts in that thread are quoted by others. I really fail to see what stuck in their craw


--------------------
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Invisiblesandman420
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. *DELETED* [Re: Infinite Monkeys] * 3
    #27855728 - 07/09/22 08:08 AM (1 year, 6 months ago)

Quote:

Infinite Monkeys said:
Is there a method to make cuts without "ripping" or "dragging" rhizo growth off of the top of agar plates?

One reason I like the punch method is because the myc doesn't seem to rip.




Yes, use a sawing motion (up-down-up-down) when you are going "across the grain" of the mycelium to prevent that tear out.


--------------------
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. *DELETED* [Re: sandman420]
    #27855734 - 07/09/22 08:14 AM (1 year, 6 months ago)

:whathesaid:



@psycho, seemed like an extreme reaction to me. I didnt read every line but seemed to me like they were the only one getting fired up. :shrug:


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Offlinesan pedro guy
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. *DELETED* [Re: LotKid] * 1
    #27855801 - 07/09/22 09:23 AM (1 year, 6 months ago)

Quote:

LotKid said:

I mean, it was in the pub, where its okay to offend, what did they expect?




I wish this were true, you can offend many people but some are a protected class….

For transfers I always take the lid off donor plate and set it down, didn’t know this was bad practice :lol: I have the tool in one had, pick up the transfer, the other hand clamshells the receiver.


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OfflineSirPsycho
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. *DELETED* [Re: san pedro guy] * 1
    #27855807 - 07/09/22 09:28 AM (1 year, 6 months ago)

I keep everything covered and "clam shell" (I really like this description btw) each container as I go.

It's not so bad to leave it open as long as you aren't passing your hand over it still is not ideal.


--------------------
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OfflineHomerSimpson
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: SirPsycho]
    #28121153 - 01/02/23 12:44 PM (1 year, 25 days ago)

Quote:

SirPsycho said:
I've using a little tool to cut perfect 1/4" circles of agar for about a year now. Kinda ghetto but very good functionality. It was a little paint brush with the bristles pulled out and a hole in the side of the metal sleeve so it doesn't suction up the transfer pieces and very thin guage so it doesn't stay hot long and melt my reusable PP5 petris.
:rollsafe:

Here's the problem though, it forces you to do one of two things in the SAB.

1. Place your hand directly over the open (big no no) plate since the tool needs to come down perpendicular to the surface to cut the little circle.

2. Remove the lid of the plate completely (another big no no) and put it aside to pick up the plate at an angle. I never actually did this one

Coincidentally... :facepalm3: I also happened to notice an increase in bacterial contam on my plates in that time period but just never made a connection.

So yeah, until I get a LFH and can "safely" open containers fully, I'm gonna stick with a knife and just slightly opening plates.




I was thinking of getting a cheap dental amalgam carrier for biopsies.
It looks ergonomic, has a nice angle so your hand wouldn't be above the plate, and just press the button to expel the biopsy. 

Images:  https://www.google.com/search?q=amalgam+carrier&source=lnms&tbm=isch&sa=X&ved=2ahUKEwjxjqbK0an8AhU7LEQIHan3CFYQ_AUoAXoECAEQAw&biw=1366&bih=601


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Invisiblefungusul
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: HomerSimpson]
    #28121229 - 01/02/23 01:51 PM (1 year, 25 days ago)

I've stopped using metal straws/x-acto for transfers after reading Syringe Neelde as Transfer Tool. Works best with longer needles(4-5").


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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: fungusul] * 1
    #28121256 - 01/02/23 02:12 PM (1 year, 25 days ago)

A pair of stainless steel tweezers is all you need, dont complicate it!

You can literally tweeze a hair off the mycelium for a transfer. No cutting, no frustration, just simplicity that works.


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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: cooleko]
    #28121413 - 01/02/23 03:43 PM (1 year, 25 days ago)

Quote:

cooleko said:
A pair of stainless steel tweezers is all you need, dont complicate it!

You can literally tweeze a hair off the mycelium for a transfer. No cutting, no frustration, just simplicity that works.



I really like that. I've just been using a scalpel now but I like that idea


--------------------
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OfflineSeventhMushroom
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: cooleko]
    #28122034 - 01/03/23 12:39 AM (1 year, 25 days ago)

Quote:

cooleko said:
A pair of stainless steel tweezers is all you need, dont complicate it!

You can literally tweeze a hair off the mycelium for a transfer. No cutting, no frustration, just simplicity that works.




Just to add my, *ahem*, extremely valuable experience, of *exactly one time*, I don't think I will use the tweezers again.

I uh, may have left a plate alone for too long, and had some nice strands on the side of the plate, so I thought "sure, why not?". I used used some tweezers to grab some of the strands individually, and used a scalpel to grab some squares out of the plate as well.

The strands grew very slowly compared to the agar samples. They consistently lagged a few days behind. Which, makes intuitive sense to me, after the fact: without any agar, there is nothing to support new growth until it gets a foothold on the new medium.

10/10 for simplicity though!


--------------------
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Edited by SeventhMushroom (01/03/23 12:40 AM)


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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: SeventhMushroom]
    #28122145 - 01/03/23 05:18 AM (1 year, 25 days ago)

A wedge has millions of cells and a tweezer full has hundreds cells. It takes time for the hundreds to catch up. Personally, I'm never in a hurry so don't mind waiting. I also dislike the look of a giant wedge in the middle of my agar, too, so am pleased with it all around.


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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: cooleko]
    #28122655 - 01/03/23 01:21 PM (1 year, 24 days ago)

:imo: When using tweezers there are some disadvantages:
- slower to get them red hot(only tip becomes red hot, remaining part that goes over dish is not sterilized)
- increased time to cool them (not ideal when using no-pour plastic dishes)
- hot handle becomes uncomfortable to touch


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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: fungusul]
    #28122678 - 01/03/23 01:42 PM (1 year, 24 days ago)

I haven't found any of those points to hold true enough to hinder me, but agree that your comments are sound.

Do you flame the handle of your scalpel? I think most people only flame what touches the agar. I flame about two centimeters of the tip and it takes 3-5 seconds. Then I only use a few mm of the tip for the transfers.

I have never worried about cooling them. Once flamed I get right to grabbing because the tips are thin enough to air cool in the distance it takes to get from flame to agar. Are you talking about the big fat tweezers? I use fine tip ones.

A few times I have noticed heat buildup when doing many transfers in a row, but nothing too hot.


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Invisiblefungusul
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: cooleko]
    #28122801 - 01/03/23 02:41 PM (1 year, 24 days ago)

Quote:

Do you flame the handle of your scalpel? I think most people only flame what touches the agar. I flame about two centimeters of the tip and it takes 3-5 seconds. Then I only use a few mm of the tip for the transfers.




I'm not using a scalpel, so I don't know how heat transfers from blade to handle.

:imo: I would flame a little bit, 5-6 cm of scalpel, but not to red hot level.

When using an exacto, flaming the blade doesn't transfer too much heat to handle's top(maybe because they are made of different metals). I'm keeping my exacto in alchool(4-5cm) before flaming, so that helps with sanitization of handle, but I do flame a little bit of the handle's top(4-5cm). 

Quote:

Are you talking about the big fat tweezers? I use fine tip ones.




Using thinner tweezers makes sense.

:imo: Using a long needle 4-5" helps because you can flame the entire needle(metalic ones).


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OfflineSirPsycho
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: cooleko] * 3
    #28122976 - 01/03/23 04:22 PM (1 year, 24 days ago)

I flame my #7 handle past the blade, to the little dip in the handle, each time I put it down and pick it back up. Only the blade between transfers if I don't put the scalpel down


--------------------
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: SirPsycho]
    #28122993 - 01/03/23 04:31 PM (1 year, 24 days ago)

You guys are overzealous with your flaming! I haven't had any issues with the more reserved approach, but if I ever get mysterious contamination I'll keep these differences in mind!


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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: cooleko]
    #28123000 - 01/03/23 04:36 PM (1 year, 24 days ago)

Definitely. Waiting for the air to settle and flaming are the two things I go ham on. But no gloves, no iso, no wiping stuff down, cutting out the soap water spray this year.


--------------------
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Offline5280feethigh
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: SirPsycho]
    #28632989 - 01/24/24 07:56 AM (3 days, 23 hours ago)

Sorry I know this thread is old but for anyone having the same issues as the op look into angled tissue punches. You can flame sterilize easy and it keeps you from having to hold your dish at an angle or hold your hand above your dish. You can get a set of 4 for pretty cheap too.


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OfflineSirPsycho
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: 5280feethigh]
    #28633004 - 01/24/24 08:10 AM (3 days, 23 hours ago)

Quote:

5280feethigh said:
Sorry I know this thread is old but for anyone having the same issues as the op look into angled tissue punches. You can flame sterilize easy and it keeps you from having to hold your dish at an angle or hold your hand above your dish. You can get a set of 4 for pretty cheap too.



That would melt through my plates, too much mass and will hold too much heat. Been using a stainless steel straw lately but still have to be careful


--------------------
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Offline5280feethigh
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: SirPsycho]
    #28633036 - 01/24/24 08:43 AM (3 days, 22 hours ago)

I haven't had any issue with melting my plates. I use a alcohol lamp and don't get it red hot.The smaller 4mm one that I use for transfers is much thinner metal also. I just thought it might address some of the issues you were having.


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OfflineSirPsycho
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: 5280feethigh]
    #28633112 - 01/24/24 09:57 AM (3 days, 21 hours ago)

Oh I flame the shit out of my tools with a propane torch. Any part of the tool that goes over the plate needs to get red hot or the little voice in my head won't stop screaming. I flame a good inch past the bend in the stainless steel straw and all the way to the dip in my #7 scalpel handle


--------------------
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Offline5280feethigh
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: SirPsycho]
    #28633145 - 01/24/24 10:26 AM (3 days, 21 hours ago)

Well that little voice definitely has a valid point. I need to get one of those straws,it's nice to try different tools. Do you need to modify it at all?


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OfflineSirPsycho
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: 5280feethigh]
    #28633148 - 01/24/24 10:31 AM (3 days, 21 hours ago)

Quote:

5280feethigh said:
Well that little voice definitely has a valid point. I need to get one of those straws,it's nice to try different tools. Do you need to modify it at all?



Yeah, you need to make a handle(heat sink) for yourself. I just wrapped a bunch of micropore tape. Stipe used Flex Seal spray. Just make sure you leave both ends of the straw open so it doesn't form a vacuum and pick up pieces


--------------------
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Balance in life is like running on ice.

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Offline5280feethigh
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: SirPsycho]
    #28633159 - 01/24/24 10:41 AM (3 days, 20 hours ago)

I will definitely give those a shot. Thank you


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