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LotKid
Never.Trust.A.Prankster



Registered: 01/07/17
Posts: 8,169
Loc: Shakedown St.
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. *DELETED* [Re: sandman420]
#27855734 - 07/09/22 08:14 AM (1 year, 6 months ago) |
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@psycho, seemed like an extreme reaction to me. I didnt read every line but seemed to me like they were the only one getting fired up.
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san pedro guy
Captain



Registered: 10/22/17
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. *DELETED* [Re: LotKid] 1
#27855801 - 07/09/22 09:23 AM (1 year, 6 months ago) |
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Quote:
LotKid said:
I mean, it was in the pub, where its okay to offend, what did they expect?
I wish this were true, you can offend many people but some are a protected classβ¦.
For transfers I always take the lid off donor plate and set it down, didnβt know this was bad practice I have the tool in one had, pick up the transfer, the other hand clamshells the receiver.
-------------------- Noob Grow Along 2022
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SirPsycho
Purple Belt in Google-Fu



Registered: 01/01/20
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. *DELETED* [Re: san pedro guy] 1
#27855807 - 07/09/22 09:28 AM (1 year, 6 months ago) |
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I keep everything covered and "clam shell" (I really like this description btw) each container as I go.
It's not so bad to leave it open as long as you aren't passing your hand over it still is not ideal.
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HomerSimpson
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: SirPsycho]
#28121153 - 01/02/23 12:44 PM (1 year, 25 days ago) |
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Quote:
SirPsycho said: I've using a little tool to cut perfect 1/4" circles of agar for about a year now. Kinda ghetto but very good functionality. It was a little paint brush with the bristles pulled out and a hole in the side of the metal sleeve so it doesn't suction up the transfer pieces and very thin guage so it doesn't stay hot long and melt my reusable PP5 petris. 
Here's the problem though, it forces you to do one of two things in the SAB.
1. Place your hand directly over the open (big no no) plate since the tool needs to come down perpendicular to the surface to cut the little circle.
2. Remove the lid of the plate completely (another big no no) and put it aside to pick up the plate at an angle. I never actually did this one
Coincidentally... I also happened to notice an increase in bacterial contam on my plates in that time period but just never made a connection.
So yeah, until I get a LFH and can "safely" open containers fully, I'm gonna stick with a knife and just slightly opening plates.
I was thinking of getting a cheap dental amalgam carrier for biopsies. It looks ergonomic, has a nice angle so your hand wouldn't be above the plate, and just press the button to expel the biopsy.
Images: https://www.google.com/search?q=amalgam+carrier&source=lnms&tbm=isch&sa=X&ved=2ahUKEwjxjqbK0an8AhU7LEQIHan3CFYQ_AUoAXoECAEQAw&biw=1366&bih=601
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fungusul
Fungus Kingdom


Registered: 07/16/20
Posts: 1,028
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: HomerSimpson]
#28121229 - 01/02/23 01:51 PM (1 year, 25 days ago) |
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I've stopped using metal straws/x-acto for transfers after reading Syringe Neelde as Transfer Tool. Works best with longer needles(4-5").
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cooleko
Augmentum provocatus

Registered: 08/15/22
Posts: 1,010
Loc: Seattle
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: fungusul] 1
#28121256 - 01/02/23 02:12 PM (1 year, 25 days ago) |
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A pair of stainless steel tweezers is all you need, dont complicate it!
You can literally tweeze a hair off the mycelium for a transfer. No cutting, no frustration, just simplicity that works.
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SirPsycho
Purple Belt in Google-Fu



Registered: 01/01/20
Posts: 6,900
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: cooleko]
#28121413 - 01/02/23 03:43 PM (1 year, 25 days ago) |
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Quote:
cooleko said: A pair of stainless steel tweezers is all you need, dont complicate it!
You can literally tweeze a hair off the mycelium for a transfer. No cutting, no frustration, just simplicity that works.
I really like that. I've just been using a scalpel now but I like that idea
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SeventhMushroom
just a tiny agar pin

Registered: 12/30/22
Posts: 55
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: cooleko]
#28122034 - 01/03/23 12:39 AM (1 year, 25 days ago) |
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Quote:
cooleko said: A pair of stainless steel tweezers is all you need, dont complicate it!
You can literally tweeze a hair off the mycelium for a transfer. No cutting, no frustration, just simplicity that works.
Just to add my, *ahem*, extremely valuable experience, of *exactly one time*, I don't think I will use the tweezers again.
I uh, may have left a plate alone for too long, and had some nice strands on the side of the plate, so I thought "sure, why not?". I used used some tweezers to grab some of the strands individually, and used a scalpel to grab some squares out of the plate as well.
The strands grew very slowly compared to the agar samples. They consistently lagged a few days behind. Which, makes intuitive sense to me, after the fact: without any agar, there is nothing to support new growth until it gets a foothold on the new medium.
10/10 for simplicity though!
-------------------- LAGM 2024
Edited by SeventhMushroom (01/03/23 12:40 AM)
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cooleko
Augmentum provocatus

Registered: 08/15/22
Posts: 1,010
Loc: Seattle
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: SeventhMushroom]
#28122145 - 01/03/23 05:18 AM (1 year, 25 days ago) |
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A wedge has millions of cells and a tweezer full has hundreds cells. It takes time for the hundreds to catch up. Personally, I'm never in a hurry so don't mind waiting. I also dislike the look of a giant wedge in the middle of my agar, too, so am pleased with it all around.
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fungusul
Fungus Kingdom


Registered: 07/16/20
Posts: 1,028
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: cooleko]
#28122655 - 01/03/23 01:21 PM (1 year, 24 days ago) |
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When using tweezers there are some disadvantages: - slower to get them red hot(only tip becomes red hot, remaining part that goes over dish is not sterilized) - increased time to cool them (not ideal when using no-pour plastic dishes) - hot handle becomes uncomfortable to touch
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cooleko
Augmentum provocatus

Registered: 08/15/22
Posts: 1,010
Loc: Seattle
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: fungusul]
#28122678 - 01/03/23 01:42 PM (1 year, 24 days ago) |
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I haven't found any of those points to hold true enough to hinder me, but agree that your comments are sound.
Do you flame the handle of your scalpel? I think most people only flame what touches the agar. I flame about two centimeters of the tip and it takes 3-5 seconds. Then I only use a few mm of the tip for the transfers.
I have never worried about cooling them. Once flamed I get right to grabbing because the tips are thin enough to air cool in the distance it takes to get from flame to agar. Are you talking about the big fat tweezers? I use fine tip ones.
A few times I have noticed heat buildup when doing many transfers in a row, but nothing too hot.
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fungusul
Fungus Kingdom


Registered: 07/16/20
Posts: 1,028
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: cooleko]
#28122801 - 01/03/23 02:41 PM (1 year, 24 days ago) |
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Quote:
Do you flame the handle of your scalpel? I think most people only flame what touches the agar. I flame about two centimeters of the tip and it takes 3-5 seconds. Then I only use a few mm of the tip for the transfers.
I'm not using a scalpel, so I don't know how heat transfers from blade to handle.
I would flame a little bit, 5-6 cm of scalpel, but not to red hot level.
When using an exacto, flaming the blade doesn't transfer too much heat to handle's top(maybe because they are made of different metals). I'm keeping my exacto in alchool(4-5cm) before flaming, so that helps with sanitization of handle, but I do flame a little bit of the handle's top(4-5cm).
Quote:
Are you talking about the big fat tweezers? I use fine tip ones.
Using thinner tweezers makes sense.
Using a long needle 4-5" helps because you can flame the entire needle(metalic ones).
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SirPsycho
Purple Belt in Google-Fu



Registered: 01/01/20
Posts: 6,900
Loc: Rent free in your head
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: cooleko] 3
#28122976 - 01/03/23 04:22 PM (1 year, 24 days ago) |
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I flame my #7 handle past the blade, to the little dip in the handle, each time I put it down and pick it back up. Only the blade between transfers if I don't put the scalpel down
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cooleko
Augmentum provocatus

Registered: 08/15/22
Posts: 1,010
Loc: Seattle
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: SirPsycho]
#28122993 - 01/03/23 04:31 PM (1 year, 24 days ago) |
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You guys are overzealous with your flaming! I haven't had any issues with the more reserved approach, but if I ever get mysterious contamination I'll keep these differences in mind!
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SirPsycho
Purple Belt in Google-Fu



Registered: 01/01/20
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: cooleko]
#28123000 - 01/03/23 04:36 PM (1 year, 24 days ago) |
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Definitely. Waiting for the air to settle and flaming are the two things I go ham on. But no gloves, no iso, no wiping stuff down, cutting out the soap water spray this year.
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Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
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5280feethigh
Stranger


Registered: 04/24/23
Posts: 63
Loc: Colorado
Last seen: 3 days, 20 hours
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: SirPsycho]
#28632989 - 01/24/24 07:56 AM (3 days, 23 hours ago) |
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Sorry I know this thread is old but for anyone having the same issues as the op look into angled tissue punches. You can flame sterilize easy and it keeps you from having to hold your dish at an angle or hold your hand above your dish. You can get a set of 4 for pretty cheap too.
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SirPsycho
Purple Belt in Google-Fu



Registered: 01/01/20
Posts: 6,900
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: 5280feethigh]
#28633004 - 01/24/24 08:10 AM (3 days, 23 hours ago) |
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Quote:
5280feethigh said: Sorry I know this thread is old but for anyone having the same issues as the op look into angled tissue punches. You can flame sterilize easy and it keeps you from having to hold your dish at an angle or hold your hand above your dish. You can get a set of 4 for pretty cheap too.
That would melt through my plates, too much mass and will hold too much heat. Been using a stainless steel straw lately but still have to be careful
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5280feethigh
Stranger


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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: SirPsycho]
#28633036 - 01/24/24 08:43 AM (3 days, 22 hours ago) |
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I haven't had any issue with melting my plates. I use a alcohol lamp and don't get it red hot.The smaller 4mm one that I use for transfers is much thinner metal also. I just thought it might address some of the issues you were having.
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SirPsycho
Purple Belt in Google-Fu



Registered: 01/01/20
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: 5280feethigh]
#28633112 - 01/24/24 09:57 AM (3 days, 21 hours ago) |
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Oh I flame the shit out of my tools with a propane torch. Any part of the tool that goes over the plate needs to get red hot or the little voice in my head won't stop screaming. I flame a good inch past the bend in the stainless steel straw and all the way to the dip in my #7 scalpel handle
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Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
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5280feethigh
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: SirPsycho]
#28633145 - 01/24/24 10:26 AM (3 days, 21 hours ago) |
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Well that little voice definitely has a valid point. I need to get one of those straws,it's nice to try different tools. Do you need to modify it at all?
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