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InvisibleLotKid
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: SirPsycho] * 4
    #27854551 - 07/08/22 09:38 AM (1 year, 6 months ago)

I've never heard that either. Mainly because i think its bullshit.


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OfflineDERRAYLD
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: LotKid] * 1
    #27854585 - 07/08/22 10:18 AM (1 year, 6 months ago)

:lol:


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InvisibleCapSlinger
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: DERRAYLD]
    #27854646 - 07/08/22 11:13 AM (1 year, 6 months ago)

:popcorn::takingnotes:


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OfflineSirPsycho
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: LotKid]
    #27854657 - 07/08/22 11:18 AM (1 year, 6 months ago)

Quote:

LotKid said:
I've never heard that either. Mainly because i think its bullshit.



What I said about transferring before the myc reaches the edge of the plate or what they said?


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InvisibleSharaVabdas
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. *DELETED* [Re: SirPsycho] * 1
    #27854720 - 07/08/22 12:17 PM (1 year, 6 months ago)

Post deleted by SharaVabdas

Reason for deletion: Shroomery is full of bigots and I'm leaving.


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InvisibleLotKid
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: SharaVabdas] * 2
    #27854739 - 07/08/22 12:27 PM (1 year, 6 months ago)

If the plate is clean neither matter. I do both all the time.

But specifically, i've never heard the "dont cut all the way to the bottom of the plate". I dont think thats a real thing. Maybe it is among the science scholars of reddit, that could be true. :shrug:


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OfflineBobbins
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: LotKid]
    #27854928 - 07/08/22 02:27 PM (1 year, 6 months ago)

I mostly work with my plates face down in the SAB and pick the filled side up and work with it at and angle such as the open side is facing somewhat downwards then it's always above my tools. Sure, contaminants may fall in the lid side, but it works pretty reliably for me. Sometimes such as when you're lifting your transfer out you will need to turn the open face upwards a bit so gravity is helping you control your transfer but I never work with the open plate on the 'floor'.


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InvisibleLotKid
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: Bobbins]
    #27854969 - 07/08/22 03:03 PM (1 year, 6 months ago)

that sounds like a pain in the ass.


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OfflineSirPsycho
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: LotKid]
    #27855009 - 07/08/22 03:48 PM (1 year, 6 months ago)

Quote:

LotKid said:
If the plate is clean neither matter. I do both all the time.

But specifically, i've never heard the "dont cut all the way to the bottom of the plate". I dont think thats a real thing. Maybe it is among the science scholars of reddit, that could be true. :shrug:



Yeah, I'm not really concerned about the edge of the plate either. Just remember reading it in a Stamets book and figured they might be getting their thing confused for the Stamets thing


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023


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InvisibleLotKid
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: SirPsycho]
    #27855123 - 07/08/22 05:39 PM (1 year, 6 months ago)

I never bothered to read stamets books. :shrug:


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OfflineSockadin
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: LotKid]
    #27855145 - 07/08/22 06:08 PM (1 year, 6 months ago)

:whathesaid:

Also mycelium grows outward in all directions so not grabbing the bottom of the plate does absolutely nothing.


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OfflineSirPsycho
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: Sockadin]
    #27855219 - 07/08/22 07:29 PM (1 year, 6 months ago)

Lmfao, y'all aren't missing out on much. The books are like 40 and 20 years old


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023


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InvisibleImatryin
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: SirPsycho]
    #27855387 - 07/08/22 09:40 PM (1 year, 6 months ago)

I can't hold a blade steady enough to cut and pick up a small wedge. I placed a sharpened 8 g transfer needle on a syringe and shaped a bicycle some as a plunger to push out the plug. It's 3.4 mm I.D.  now that I think about it I've had more contams than I'd like to see. I previously tried an innoc loop but it flexed too much. I'm going to try the clay sculpture tools.


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InvisibleGuerrilla
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: SirPsycho] * 1
    #27855650 - 07/09/22 06:46 AM (1 year, 6 months ago)

Quote:

SirPsycho said:
Quote:

Guerrilla said:
My friend, these metal straws work great and completely avoid the issues mentioned in your post.





I've looked at those a few times, they look like a lot of metal. Do they stay hot a long time?




No, less than a scalpel. Never gets too hot to handle, heat doesn't transfer up the straw even in long transfer sessions.


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OfflineBrian Jones
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. *DELETED* [Re: SharaVabdas]
    #27855657 - 07/09/22 06:54 AM (1 year, 6 months ago)

Quote:

SharaVabdas said:
Post deleted by SharaVabdas

Reason for deletion: Shroomery is full of bigots and I'm leaving.




I never noticed anything like that in the cultivation forum.


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InvisibleGuerrilla
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. *DELETED* [Re: Brian Jones] * 1
    #27855661 - 07/09/22 07:00 AM (1 year, 6 months ago)

Quote:

Brian Jones said:
Quote:

SharaVabdas said:
Post deleted by SharaVabdas

Reason for deletion: Shroomery is full of bigots and I'm leaving.




I never noticed anything like that in the cultivation forum.




He's deleted all 489 of his posts with the same comment! Whoa


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Being pissed on does not make you a real man.


Edited by Guerrilla (07/09/22 07:00 AM)


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InvisibleLotKid
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. *DELETED* [Re: Guerrilla] * 4
    #27855673 - 07/09/22 07:13 AM (1 year, 6 months ago)

I got curious and looked up his/hers/their/its posts. And they were posting in the pub on a trans woman thread and got offended by looks of it. I dont see what was so offensive but appearently sex and gender was more important to them then every other aspect of this site.

I mean, it was in the pub, where its okay to offend, what did they expect?


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InvisibleInfinite Monkeys

Registered: 09/01/21
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. *DELETED* [Re: SirPsycho]
    #27855711 - 07/09/22 07:52 AM (1 year, 6 months ago)

Is there a method to make cuts without "ripping" or "dragging" rhizo growth off of the top of agar plates?

One reason I like the punch method is because the myc doesn't seem to rip.


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OfflineSirPsycho
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. *DELETED* [Re: LotKid]
    #27855716 - 07/09/22 07:58 AM (1 year, 6 months ago)

Quote:

Guerrilla said:
Quote:

SirPsycho said:
Quote:

Guerrilla said:
My friend, these metal straws work great and completely avoid the issues mentioned in your post.





I've looked at those a few times, they look like a lot of metal. Do they stay hot a long time?




No, less than a scalpel. Never gets too hot to handle, heat doesn't transfer up the straw even in long transfer sessions.



I'll grab a set and give them a go as well.
Quote:

LotKid said:
I got curious and looked up his/hers/their/its posts. And they were posting in the pub on a trans woman thread and got offended by looks of it. I dont see what was so offensive but appearently sex and gender was more important to them then every other aspect of this site.

I mean, it was in the pub, where its okay to offend, what did they expect?



Yep, I did the same. Parts of their posts in that thread are quoted by others. I really fail to see what stuck in their craw


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023


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Invisiblesandman420
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Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. *DELETED* [Re: Infinite Monkeys] * 3
    #27855728 - 07/09/22 08:08 AM (1 year, 6 months ago)

Quote:

Infinite Monkeys said:
Is there a method to make cuts without "ripping" or "dragging" rhizo growth off of the top of agar plates?

One reason I like the punch method is because the myc doesn't seem to rip.




Yes, use a sawing motion (up-down-up-down) when you are going "across the grain" of the mycelium to prevent that tear out.


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