Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Kraken Kratom Red Vein Kratom   PhytoExtractum Maeng Da Thai Kratom Leaf Powder

Jump to first unread post Pages: 1 | 2 | 3 | 4 | Next >  [ show all ]
OfflineSirPsycho
Purple Belt in Google-Fu
I'm a teapot User Gallery


Registered: 01/01/20
Posts: 6,900
Loc: Rent free in your head
Last seen: 9 hours, 13 minutes
Trusted Cultivator
I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. * 2
    #27853904 - 07/07/22 06:24 PM (1 year, 6 months ago)

I've using a little tool to cut perfect 1/4" circles of agar for about a year now. Kinda ghetto but very good functionality. It was a little paint brush with the bristles pulled out and a hole in the side of the metal sleeve so it doesn't suction up the transfer pieces and very thin guage so it doesn't stay hot long and melt my reusable PP5 petris.
:rollsafe:

Here's the problem though, it forces you to do one of two things in the SAB.

1. Place your hand directly over the open (big no no) plate since the tool needs to come down perpendicular to the surface to cut the little circle.

2. Remove the lid of the plate completely (another big no no) and put it aside to pick up the plate at an angle. I never actually did this one

Coincidentally... :facepalm3: I also happened to notice an increase in bacterial contam on my plates in that time period but just never made a connection.

So yeah, until I get a LFH and can "safely" open containers fully, I'm gonna stick with a knife and just slightly opening plates.


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023


Extras: Filter Print Post Top
OfflineSockadin
Male User Gallery


Registered: 01/03/10
Posts: 7,244
Last seen: 2 months, 19 days
Trusted Cultivator
Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: SirPsycho] * 2
    #27853914 - 07/07/22 06:31 PM (1 year, 6 months ago)



Extras: Filter Print Post Top
OfflineSirPsycho
Purple Belt in Google-Fu
I'm a teapot User Gallery


Registered: 01/01/20
Posts: 6,900
Loc: Rent free in your head
Last seen: 9 hours, 13 minutes
Trusted Cultivator
Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: Sockadin]
    #27853915 - 07/07/22 06:34 PM (1 year, 6 months ago)

I like it, I use a loop to cut bits of agar out of 15ml slant tubes. I can get a bit thicker guage wire and bend it up all how I need. You're a gentleman and a scholar fine sir, thank you


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023


Extras: Filter Print Post Top
OfflineSockadin
Male User Gallery


Registered: 01/03/10
Posts: 7,244
Last seen: 2 months, 19 days
Trusted Cultivator
Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: SirPsycho] * 1
    #27853920 - 07/07/22 06:37 PM (1 year, 6 months ago)

Gotta be honest I haven't had to flame sterilize in a year and have very few issues other than drunken transfers that occasionally get ditched. And thank you for your compliment. I do enjoy your posts.


Extras: Filter Print Post Top
OfflineSirPsycho
Purple Belt in Google-Fu
I'm a teapot User Gallery


Registered: 01/01/20
Posts: 6,900
Loc: Rent free in your head
Last seen: 9 hours, 13 minutes
Trusted Cultivator
Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: Sockadin]
    #27853927 - 07/07/22 06:40 PM (1 year, 6 months ago)

Oh I plan on sticking it on the end of an x-acto handle and flaming the hell out of it.

Also, thank you as well.


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023


Extras: Filter Print Post Top
InvisibleGuerrilla
Bumbaclart


Registered: 01/30/21
Posts: 3,170
Loc: United Kingdom Flag
Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: SirPsycho] * 2
    #27853938 - 07/07/22 06:49 PM (1 year, 6 months ago)

My friend, these metal straws work great and completely avoid the issues mentioned in your post.



--------------------
Being pissed on does not make you a real man.


Edited by Guerrilla (07/07/22 06:51 PM)


Extras: Filter Print Post Top
InvisibleElf_on_a_Log
Elf

Registered: 07/13/14
Posts: 610
Loc: On a log
Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: SirPsycho] * 1
    #27853960 - 07/07/22 07:09 PM (1 year, 6 months ago)

Quote:

SirPsycho said:
1. Place your hand directly over the open (big no no) plate since the tool needs to come down perpendicular to the surface to cut the little circle.



Have you tried holding the transferring plate sideways or top-angled-down?  The receiving plate can't be at a steep angle for obvious reasons but doing that with the transferring plate greatly cuts contam chances.

That no-flame paperclip method is interesting.  Going to have to give that a try sometime.


--------------------
"I was strolling through the woods one day when I came upon an elf sitting on a log.  He offered me a strange-looking mushroom to eat.  As soon as I ate it, I realized that I was the elf on the log and that my human life had been but a dream."
- Elf_on_a_Log



LAGM 2.022 Grow Log


Extras: Filter Print Post Top
OfflineSirPsycho
Purple Belt in Google-Fu
I'm a teapot User Gallery


Registered: 01/01/20
Posts: 6,900
Loc: Rent free in your head
Last seen: 9 hours, 13 minutes
Trusted Cultivator
Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: Guerrilla]
    #27853980 - 07/07/22 07:39 PM (1 year, 6 months ago)

Quote:

Guerrilla said:
My friend, these metal straws work great and completely avoid the issues mentioned in your post.





I've looked at those a few times, they look like a lot of metal. Do they stay hot a long time?


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023


Extras: Filter Print Post Top
OfflineSirPsycho
Purple Belt in Google-Fu
I'm a teapot User Gallery


Registered: 01/01/20
Posts: 6,900
Loc: Rent free in your head
Last seen: 9 hours, 13 minutes
Trusted Cultivator
Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: Elf_on_a_Log]
    #27853981 - 07/07/22 07:41 PM (1 year, 6 months ago)

Quote:

Elf_on_a_Log said:
Quote:

SirPsycho said:
1. Place your hand directly over the open (big no no) plate since the tool needs to come down perpendicular to the surface to cut the little circle.



Have you tried holding the transferring plate sideways or top-angled-down?  The receiving plate can't be at a steep angle for obvious reasons but doing that with the transferring plate greatly cuts contam chances.

That no-flame paperclip method is interesting.  Going to have to give that a try sometime.



I have, it's a bit un-widely for my liking while holding onto the lid with the same hand.


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023


Extras: Filter Print Post Top
OfflineCocaineBuffet
Stranger
 User Gallery


Registered: 08/29/19
Posts: 3,455
Last seen: 7 hours, 20 minutes
Trusted Cultivator
Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: SirPsycho] * 5
    #27854098 - 07/07/22 09:42 PM (1 year, 6 months ago)

I just cut squares or triangles out of the donating plate to transfer. I think there are some benefits to using a punch but there are too many vectors to make me feel like I need to switch.

Nice uniform plates are dope don't get me wrong but they are hardly necessary.


Extras: Filter Print Post Top
Offline99.99
Stranger
 User Gallery

Registered: 12/22/14
Posts: 1,933
Last seen: 7 hours, 31 minutes
Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: SirPsycho] * 1
    #27854112 - 07/07/22 09:59 PM (1 year, 6 months ago)

I use clay sculpture tools…. you can wrap them up in foil and sterilize them the same time you’re doing your agar



Amazon and eBay has these for a couple bucks a set


Extras: Filter Print Post Top
InvisibleThe Fresh PrintsS
Smell ya later
Male User Gallery


Registered: 05/19/12
Posts: 1,377
Loc: Bel-Air
Trusted Cultivator
Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: 99.99] * 1
    #27854146 - 07/07/22 10:48 PM (1 year, 6 months ago)

Definitely glad you made the distinction for SAB work. Punch tools are great in front of the hood, but the SAB is a whole different ball game


--------------------


Extras: Filter Print Post Top
OfflineSirPsycho
Purple Belt in Google-Fu
I'm a teapot User Gallery


Registered: 01/01/20
Posts: 6,900
Loc: Rent free in your head
Last seen: 9 hours, 13 minutes
Trusted Cultivator
Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: CocaineBuffet]
    #27854307 - 07/08/22 04:55 AM (1 year, 6 months ago)

Quote:

99.99 said:
I use clay sculpture tools…. you can wrap them up in foil and sterilize them the same time you’re doing your agar



Amazon and eBay has these for a couple bucks a set



When I use the inoculation loop to grab agar out of slants it's used in a similar fashion to those tools. I'll cut away ribbons of agar by dragging the loop across the surface

Quote:

The Fresh Prints said:
Definitely glad you made the distinction for SAB work. Punch tools are great in front of the hood, but the SAB is a whole different ball game



They are ok in the SAB too just not really best practice. It did increase my contam rate but just slightly, hence taking a whole damn year to put 2 and 2 together.

Quote:

CocaineBuffet said:
I just cut squares or triangles out of the donating plate to transfer. I think there are some benefits to using a punch but there are too many vectors to make me feel like I need to switch.

Nice uniform plates are dope don't get me wrong but they are hardly necessary.




Beyond pretty plates, I also found it really handy when doing lots of A2G. Only been doing A2G into pints of millet lately though, I then use the millet to noc other stuff. So I've kinda lost out on the A2G benefit of it too.

Pretty much just left with another couple of vectors and some not so best practice.

Gonna give the paper clip loop a shot, seems to solve all my handling issues and I'm not worried about melting through plates or burning my hands, which are the reasons I've avoided the straws Guerilla posted.


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023


Extras: Filter Print Post Top
OfflineDERRAYLD
Constructus
Male User Gallery

Registered: 05/13/02
Posts: 9,281
Loc: South Africa
Last seen: 46 minutes, 48 seconds
Trusted Cultivator
Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: SirPsycho] * 2
    #27854309 - 07/08/22 04:56 AM (1 year, 6 months ago)

I use punches quite rarely as well, the circle looks good but the growth is always very similar to a triangle without the added vector.


Edited by DERRAYLD (07/08/22 06:38 AM)


Extras: Filter Print Post Top
InvisibleLotKid
Never.Trust.A.Prankster
Male User Gallery


Registered: 01/07/17
Posts: 8,169
Loc: Shakedown St.
Trusted Cultivator
Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: DERRAYLD] * 4
    #27854344 - 07/08/22 06:08 AM (1 year, 6 months ago)

#7 scalpel handle and #11 blades for the win.

I've never found the hole punch method appealing.


--------------------

:mushroom2: LotKid's PE Tips        :shpongle:    LotKid's Chocolates :mushroom2:
:dancingbear: :dancingbear:


Extras: Filter Print Post Top
OfflineSirPsycho
Purple Belt in Google-Fu
I'm a teapot User Gallery


Registered: 01/01/20
Posts: 6,900
Loc: Rent free in your head
Last seen: 9 hours, 13 minutes
Trusted Cultivator
Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: LotKid]
    #27854377 - 07/08/22 06:47 AM (1 year, 6 months ago)

Well damn, Jackie!

I just made my way through the 1000 x-acto blades I got when I started and ordered a proper scalpel yesterday but I went with a #3 handle


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023


Extras: Filter Print Post Top
InvisibleLotKid
Never.Trust.A.Prankster
Male User Gallery


Registered: 01/07/17
Posts: 8,169
Loc: Shakedown St.
Trusted Cultivator
Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: SirPsycho] * 3
    #27854457 - 07/08/22 08:18 AM (1 year, 6 months ago)

get yourself a #7 handle. it has the longest reach. and #11 blades have a nice point on them for spearing your transfers.


--------------------

:mushroom2: LotKid's PE Tips        :shpongle:    LotKid's Chocolates :mushroom2:
:dancingbear: :dancingbear:


Extras: Filter Print Post Top
OfflineSpirit-Crusher
DOOM HIPPIE


Registered: 04/24/19
Posts: 358
Loc: PNW
Last seen: 15 days, 3 minutes
Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: LotKid]
    #27854490 - 07/08/22 08:38 AM (1 year, 6 months ago)

Quote:

LotKid said:
get yourself a #7 handle. it has the longest reach. and #11 blades have a nice point on them for spearing your transfers.



This is what I use as well.

I got caught up in trying to use a punch for like a minute. My agar always got stuck in the punch hole. Then I realized it was a waste of time for me. I just use squares and my plates look just fine, but even more important, they're functional.


Extras: Filter Print Post Top
InvisibleSharaVabdas
Male User Gallery


Registered: 03/23/22
Posts: 489
Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. *DELETED* [Re: SirPsycho]
    #27854493 - 07/08/22 08:40 AM (1 year, 6 months ago)

Post deleted by SharaVabdas

Reason for deletion: Shroomery is full of bigots and I'm leaving.


Extras: Filter Print Post Top
OfflineSirPsycho
Purple Belt in Google-Fu
I'm a teapot User Gallery


Registered: 01/01/20
Posts: 6,900
Loc: Rent free in your head
Last seen: 9 hours, 13 minutes
Trusted Cultivator
Re: I stopped using punch tools to cut agar transfer pieces in the SAB cause I think it's bad practice. [Re: SharaVabdas]
    #27854521 - 07/08/22 09:05 AM (1 year, 6 months ago)

Quote:

LotKid said:
get yourself a #7 handle. it has the longest reach. and #11 blades have a nice point on them for spearing your transfers.



Yeah, I will and those are the blades I got

Quote:

SharaVabdas said:

You're not really supposed to cut to the bottom of the agar, you're supposed to just grab a thin, top layer to minimize contact with uncolonized agar from the source plate.




Never heard that before.

I know it's best practice to transfer while the myc is still 1/4" away from the edge of the plate and you're not supposed to transfer that uncolonized agar since the edges of the plate are where contam is most likely.


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023


Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 | 3 | 4 | Next >  [ show all ]

Shop: Kraken Kratom Red Vein Kratom   PhytoExtractum Maeng Da Thai Kratom Leaf Powder


Similar ThreadsPosterViewsRepliesLast post
* Any Tips on Agar Transfers Haste 148 5 01/24/24 07:09 AM
by Haste
* How to make agar 2 agar transfer? esp 6,652 7 01/08/04 11:08 AM
by YidakiMan
* agar transfers *DELETED* thisone 1,245 3 11/20/03 08:58 PM
by Mycomancer
* Innoculation with agar wedge Visigoth 5,276 6 01/15/23 12:59 PM
by Screwup
* agar questions stara 1,356 6 04/21/04 12:29 PM
by stara
* Benefits of Agar? celsius 9,379 10 12/01/19 12:36 AM
by wildernessjunkie
* Re: mold in agar plate Anonymous 12,180 3 12/04/99 12:35 AM
by Anonymous
* NEW Tissue Culture Technique - No agar, No flow hood, easy as hell
( 1 2 all )
funkyballoon 21,280 38 05/11/01 11:08 AM
by MAIA

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
2,420 topic views. 30 members, 168 guests and 29 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.032 seconds spending 0.01 seconds on 14 queries.