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InvisibleSharaVabdas
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Refrigerated, Long-Term Storage of Cold-Intolerant Species on Sorghum. *DELETED* * 1
    #27728033 - 04/09/22 09:12 PM (1 year, 9 months ago)

Post deleted by SharaVabdas

Reason for deletion: Shroomery is full of bigots and I'm leaving.


Edited by SharaVabdas (04/09/22 09:39 PM)


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Offlinenerdintheshell
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Re: Refrigerated, Long-Term Storage of Cold-Intolerant Species on Sorghum. [Re: SharaVabdas] * 2
    #27728182 - 04/09/22 11:20 PM (1 year, 9 months ago)

Quote:

I'm not certain how retail vendors of these cultures keep their monocultures fresh over the years




My guess is glycerin stocks, at least that's what mycelia uses.


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InvisibleSpeeker

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Re: Refrigerated, Long-Term Storage of Cold-Intolerant Species on Sorghum. [Re: SharaVabdas] * 2
    #27728413 - 04/10/22 07:33 AM (1 year, 9 months ago)

> as any pink oyster grower will know, is that they can not be refrigerated without dying.
I have kept them in a fridge and they were always okay. Also sent some old wedges around straight from the fridge with no complains.
So, this isn't always true. Probably a strain question then. I didn't like its taste nor the yellow oyster, so they're gone..

edit:
or are they? I still have this original package (thick plastic) sent by expertcontamgrower, I hope I remembered his name right.

Some cultures I have noticed just weirdly survive no matter what..


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Edited by Speeker (04/10/22 07:59 AM)


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InvisibleSharaVabdas
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Re: Refrigerated, Long-Term Storage of Cold-Intolerant Species on Sorghum. *DELETED* [Re: SharaVabdas] * 1
    #27737510 - 04/16/22 03:58 PM (1 year, 9 months ago)

Post deleted by SharaVabdas

Reason for deletion: Shroomery is full of bigots and I'm leaving.


Edited by SharaVabdas (04/16/22 04:27 PM)


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OfflineAshleyRPlayer
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Re: Refrigerated, Long-Term Storage of Cold-Intolerant Species on Sorghum. [Re: SharaVabdas] * 1
    #27756010 - 04/29/22 01:59 AM (1 year, 8 months ago)

The ability of sorghum genotypes to germinate, thrive, and generate sufficient grain yields in cold (but above freezing) air and soil temperatures is referred to as cold tolerance. Cold tolerance sources were explored, as well as their utility in grain sorghum. When graded at physiological maturity, 39 of 380 excessively tall, photoperiod-sensitive, and late-maturing accessions acquired from China, Ethiopia, and Uganda were cold-tolerant, 757 moderately tolerant, and the rest were vulnerable. The accessions from Ethiopia (e.g. Alemaya 70, Jejewegere 935, and Muyra) and Uganda (e.g. Alemaya 70, Jejewegere 935, and Muyra) have the highest levels of cold tolerance (Mabere, Magune, and Nyundo). A dominant attribute appears to be cold tolerance. However, there was a wide range of variation among cold-tolerant genotypes in cold-susceptible x cold-tolerant crosses, and the frequency of highly tolerant segregates was less than lAVo in the Fr, implying that several genes controlled the character's inheritance and that their effects were largely cumulative. Dwarf, photoperiod-insensitive, early-maturing, and cold-tolerant experimental lines were created in significant numbers. Some of them had adapted well to the highlands of Honduras, Kenya, and Mexico. They were often unsuited to high-latitude environments in the northwest United States, southern Canada, and West Germany.


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InvisibleSharaVabdas
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Re: Refrigerated, Long-Term Storage of Cold-Intolerant Species on Sorghum. *DELETED* [Re: AshleyRPlayer] * 1
    #27756287 - 04/29/22 08:51 AM (1 year, 8 months ago)

Post deleted by SharaVabdas

Reason for deletion: Shroomery is full of bigots and I'm leaving.


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Offlinetrippleblack
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Re: Refrigerated, Long-Term Storage of Cold-Intolerant Species on Sorghum. [Re: SharaVabdas] * 2
    #27757252 - 04/30/22 07:00 AM (1 year, 8 months ago)

I'm not certain how retail vendors of these cultures keep their monocultures fresh over the years  -most of them dont lol 



interested in seeing if this work. I have gotten very degraded cultures of pink oyster in the past. I took expensive measures in trails to store cultures very long term, specifically cordycep, which will sensescene over nothing.  What i'm doing is freeze drying/ lyopholization, which is easy once you have the machine and technique.  If this works well for you, it's something i would love to consider using as a mid range backup stored in culture tubes.


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OfflineShu
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Re: Refrigerated, Long-Term Storage of Cold-Intolerant Species on Sorghum. [Re: SharaVabdas] * 1
    #27769415 - 05/09/22 04:40 AM (1 year, 8 months ago)

:popcorn:


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InvisibleSharaVabdas
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Re: Refrigerated, Long-Term Storage of Cold-Intolerant Species on Sorghum. *DELETED* [Re: SharaVabdas]
    #27783534 - 05/18/22 08:33 PM (1 year, 8 months ago)

Post deleted by SharaVabdas

Reason for deletion: Shroomery is full of bigots and I'm leaving.


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InvisibleSharaVabdas
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Re: Refrigerated, Long-Term Storage of Cold-Intolerant Species on Sorghum. *DELETED* [Re: SharaVabdas]
    #27836753 - 06/25/22 08:51 PM (1 year, 6 months ago)

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Reason for deletion: Shroomery is full of bigots and I'm leaving.


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Offlinecozmyc
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Re: Refrigerated, Long-Term Storage of Cold-Intolerant Species on Sorghum. [Re: SharaVabdas] * 1
    #27836805 - 06/25/22 09:42 PM (1 year, 6 months ago)

Yay :popcorn:


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InvisibleSharaVabdas
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Re: Refrigerated, Long-Term Storage of Cold-Intolerant Species on Sorghum. *DELETED* [Re: SharaVabdas]
    #27840400 - 06/28/22 10:22 AM (1 year, 6 months ago)

Post deleted by SharaVabdas

Reason for deletion: Shroomery is full of bigots and I'm leaving.


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OfflineShu
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Re: Refrigerated, Long-Term Storage of Cold-Intolerant Species on Sorghum. [Re: SharaVabdas]
    #27843438 - 06/30/22 06:38 AM (1 year, 6 months ago)

What temp is your refrigerator?


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InvisibleSharaVabdas
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Re: Refrigerated, Long-Term Storage of Cold-Intolerant Species on Sorghum. *DELETED* [Re: SharaVabdas]
    #27843628 - 06/30/22 09:52 AM (1 year, 6 months ago)

Post deleted by SharaVabdas

Reason for deletion: Shroomery is full of bigots and I'm leaving.


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OfflineEclipse3130
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Re: Refrigerated, Long-Term Storage of Cold-Intolerant Species on Sorghum. [Re: SharaVabdas]
    #27843727 - 06/30/22 11:43 AM (1 year, 6 months ago)

Why are you trying to refrigerate a cold intolerant species just out of curiosity. Keep everything in room temp


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"In The Material World One seeks retirement and grows Old
In The Magical World One seeks Enlightenment and grows Wiser
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InvisibleSharaVabdas
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Re: Refrigerated, Long-Term Storage of Cold-Intolerant Species on Sorghum. *DELETED* [Re: Eclipse3130]
    #27844738 - 07/01/22 08:47 AM (1 year, 6 months ago)

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OfflineEclipse3130
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Re: Refrigerated, Long-Term Storage of Cold-Intolerant Species on Sorghum. [Re: SharaVabdas]
    #27845789 - 07/01/22 09:42 PM (1 year, 6 months ago)

Quote:

SharaVabdas said:
Quote:

Eclipse3130 said:
Why are you trying to refrigerate a cold intolerant species just out of curiosity. Keep everything in room temp




It's pretty thoroughly explained in the first post of this thread. There is a whole "Why Though?" section up there.

It's a matter of protecting a given culture from rapid senescence. Same reason we refrigerate any culture.




That's what I mean, I don't refrigerate anything, especially cubes. I store cubes in room temp. Refrigeration doesn't do anything beneficial especially for cold intolerant species. Slants are air tight when screwed down and the mycelium stalls naturally on its own for years without having to subject them to low temps which actually mostly just damage the mycelium further


--------------------
"In The Material World One seeks retirement and grows Old
In The Magical World One seeks Enlightenment and grows Wiser
In The Miraculous World One seeks nothing and grows Lighter
As we all tread the Homeward Path we will explore many Realms
And one day... we will all Realize that all experiences are Simply
Different ways in which The
All-That Is
Perceives Itself"


Edited by Eclipse3130 (07/02/22 12:13 AM)


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OfflineShu
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Re: Refrigerated, Long-Term Storage of Cold-Intolerant Species on Sorghum. [Re: Eclipse3130] * 1
    #27846167 - 07/02/22 08:23 AM (1 year, 6 months ago)

Quote:

Eclipse3130 said:...
mycelium stalls naturally on its own for years without having to subject them to low temps which actually mostly just damage the mycelium further



This is contrary to every microbiology lab practice I'm aware of. Do you have a reference?


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OfflineEclipse3130
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Re: Refrigerated, Long-Term Storage of Cold-Intolerant Species on Sorghum. [Re: Shu]
    #27846264 - 07/02/22 10:07 AM (1 year, 6 months ago)

Temperatures can definitely slow down mycelium from growing as well but it's unnecessary especially for cold intolerant species, it is not needed to effectively "stall" mycelium into a dormant state. Lack of gas exchange will do that all on its own no refrigeration needed.

I store slants in room temp and it works well, if I want to stall an agar plate for a longer duration I'll vac seal it(I'll even sometimes vac seal slants for further protection) this is to help reduce any excess gas exchange that could result in dehydrating your agar, usually only an issue with plates as they don't have a tight seal.

I have some slants from 1.5 years ago in room temp that I just transfered and are recovering nicely.


--------------------
"In The Material World One seeks retirement and grows Old
In The Magical World One seeks Enlightenment and grows Wiser
In The Miraculous World One seeks nothing and grows Lighter
As we all tread the Homeward Path we will explore many Realms
And one day... we will all Realize that all experiences are Simply
Different ways in which The
All-That Is
Perceives Itself"


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