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InvisibleCreonAntigone
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Registered: 05/30/21
Posts: 2,971
Detection of endophyte from conidia content in plant matter
    #27838055 - 06/26/22 08:33 PM (1 year, 10 months ago)

In my research, it seems that many endophytes can live within plants without being easily detectable. Using one of the obvious examples, ergot, it can easily live in a plant without being noticeable unless it fruits.

So, let us say there are a cluster of grasses, and only one has a detectable ergot fruit; however, I am fairly certain that at the least, the surrounding grasses will have a latent endophyte infection. Presumably, the plant matter will contain asexual spores, conidia, which are produced readily by ergot (as by other endophytes) even in the absence of fruits.

My question is, supposing that I know exactly what species I'm looking for and have a very high confidence that the plant is highly infected - so, in this example, grasses very close to grasses that contain ergot fruits - is there a method that could allow the processing of the plant material to see whether or not there are any conidia in the plants?

I know very well that spores can easily be detected by microscopy. Presumably, even without fruits, I might be able to find areas that are likely to contain a high amount of endophyte conidia - let us say, in the case of ergot, plant ovaries that are darkening, or that contain honeydew. Is there any tried and true method that could allow me to tell the species from just these conidia, even without fruits? How could I test it?

It might be prudent to culture the material somehow to multiply it and get a guess at the species, if the endophyte is capable of growing in culture.

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OfflineJawn802
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Registered: 07/14/22
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Last seen: 1 year, 9 months
Re: Detection of endophyte from conidia content in plant matter [Re: CreonAntigone]
    #27862417 - 07/14/22 11:59 AM (1 year, 9 months ago)

You could probably design a primer unique to ergot and then use pcr and gel electrophoresis  to tell if it is present in the plant material

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OfflineMr_Mushrooms
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Registered: 07/29/22
Posts: 87
Last seen: 1 year, 9 months
Re: Detection of endophyte from conidia content in plant matter [Re: CreonAntigone]
    #27882937 - 07/30/22 01:57 PM (1 year, 9 months ago)

This is really interesting and would be highly useful if you could let us know when you find out anything and even post a tek and pictures! Thanks for doing this! Following thread

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Invisibleellomello
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Registered: 08/11/08
Posts: 2,435
Loc: babilonUSA
Re: Detection of endophyte from conidia content in plant matter [Re: CreonAntigone]
    #27929299 - 09/01/22 04:33 PM (1 year, 8 months ago)

not sure if it would work, maybe a color drop test could detect them..
colormetric drop test

-test a sample known to contain conidia, hopefully get a unique color,
-compared to another control sample that is known not to contain them.
-then test suspected samples to see if you get the same unique color.


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