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OfflineBlueAndOrangeS
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Question about inoculation loops
    #27833258 - 06/23/22 06:31 PM (1 day, 21 hours ago)

I searched and found nothing. It appears that nichrome loops are not heatable with an induction coil sterilizer. I’m coming up empty finding loops that would be. I really love how easy and fast it is to work with the coil with a footswitch. Anyone have any recommendations that might help me keep an open flame out of my lab?


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OfflineAgentClaret
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Re: Question about inoculation loops [Re: BlueAndOrange] * 2
    #27833275 - 06/23/22 06:44 PM (1 day, 21 hours ago)

Make your own! I used an Xacto handle and a piece of vape coil wire that I looped and braided at the end.


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OnlineKROM
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Re: Question about inoculation loops [Re: BlueAndOrange]
    #27833277 - 06/23/22 06:46 PM (1 day, 21 hours ago)

I have no idea if it would work, but maybe a stainless steel dermatology loop? Might be worth a shot. I apologize, but this is the best picture I could find:

Full disclosure, it might be a terrible idea.

Conversely, you could sterilize cotton swabs wrapped in foil, as seen here. They work quite well for swabbing plates!

The vape coil x-acto handle that agent claret suggested also sounds like a good solution.


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InvisibleMycoRob
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Re: Question about inoculation loops [Re: BlueAndOrange]
    #27833375 - 06/23/22 07:49 PM (1 day, 20 hours ago)

May just have to use the disposable plastic ones for a bit until you find one to fit your situation


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OfflineBlueAndOrangeS
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Re: Question about inoculation loops [Re: MycoRob]
    #27833431 - 06/23/22 08:23 PM (1 day, 19 hours ago)

I’m going to use the nichrome with a flame for now while I search for something compatible with my sterilizer.


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OfflineTanavast
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Re: Question about inoculation loops [Re: BlueAndOrange]
    #27833436 - 06/23/22 08:27 PM (1 day, 19 hours ago)

So uh, what’s an inoculation loop bros?


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OfflineBlueAndOrangeS
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Re: Question about inoculation loops [Re: Tanavast]
    #27833439 - 06/23/22 08:30 PM (1 day, 19 hours ago)

It’s a little loop of metal for inoculating agar (or other things I guess) with spores from sporeprints or even syringes. You can make one out of a paper clip if you wanted. They’re just a little piece of wire bent into a loop at the end. The loop protects you from damaging spores.


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OfflineTanavast
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Re: Question about inoculation loops [Re: BlueAndOrange]
    #27833467 - 06/23/22 08:47 PM (1 day, 19 hours ago)

So you “scrape” some spores onto the loop then gently rub them  onto the agar?

I haven’t ventured into the agar world having mostly done pf tek grows and ground brf cakes to bulk but agar is the next journey, any info on your techniques would be appreciated friend!


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InvisibleMycoRob
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Re: Question about inoculation loops [Re: Tanavast]
    #27833473 - 06/23/22 08:49 PM (1 day, 19 hours ago)

It’s basically just a little tool to scrape the spores off of a spore print onto agar


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OfflineTanavast
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Re: Question about inoculation loops [Re: MycoRob]
    #27833483 - 06/23/22 08:52 PM (1 day, 19 hours ago)

And the reason you don’t want to use a blade is that the blade will damage some spores?


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OfflineMushit
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Re: Question about inoculation loops [Re: Tanavast]
    #27833493 - 06/23/22 09:01 PM (1 day, 18 hours ago)

Quote:

Tanavast said:
And the reason you don’t want to use a blade is that the blade will damage some spores?



A blade works, but it is easier to spread spores around on a plate with a loop than a blade, IMHO.


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OfflineTanavast
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Re: Question about inoculation loops [Re: Mushit]
    #27833667 - 06/23/22 10:49 PM (1 day, 17 hours ago)

Quote:

Mushit said:
Quote:

Tanavast said:
And the reason you don’t want to use a blade is that the blade will damage some spores?



A blade works, but it is easier to spread spores around on a plate with a loop than a blade, IMHO.



You just made it click in my brain.  Makes a lot of sense thank you!


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OfflineBobbins
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Re: Question about inoculation loops [Re: Tanavast]
    #27833885 - 06/24/22 03:04 AM (1 day, 12 hours ago)

These can also be used to transfer mycelium around.


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OfflineThe Tao
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Re: Question about inoculation loops [Re: Bobbins]
    #27833973 - 06/24/22 07:46 AM (1 day, 8 hours ago)

Quote:

BlueAndOrange said:
I searched and found nothing. It appears that nichrome loops are not heatable with an induction coil sterilizer. I’m coming up empty finding loops that would be. I really love how easy and fast it is to work with the coil with a footswitch. Anyone have any recommendations that might help me keep an open flame out of my lab?



Make your own loops using steel tie wire. Cut off about 8 inches of wire. Loop it around a screwdriver. Hold the ends with pliers and twist.




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OfflineBlueAndOrangeS
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Re: Question about inoculation loops [Re: The Tao]
    #27834075 - 06/24/22 10:09 AM (1 day, 5 hours ago)

I guess I’ll do exactly that. Thanks.


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Offlinerumfor69
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Re: Question about inoculation loops [Re: BlueAndOrange]
    #27834101 - 06/24/22 10:30 AM (1 day, 5 hours ago)

A loops primary use is for streaking plates.
You make a series of streaks going one way
then streak across those streaks going an
opposite way. This allows spores to be put
in lines which will give more points to
choose from later and help isolate down
genetics a little faster.


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OfflineThe Tao
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Re: Question about inoculation loops [Re: BlueAndOrange] * 1
    #27834116 - 06/24/22 10:41 AM (1 day, 5 hours ago)

Get the cheap black wire and use a magnet to confirm it has ferromagnetic properties. Otherwise it won’t work with induction.


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