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Psilosophy23 said:
This is nuts, so basically that is a species hybrid right ?

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Psilosophy23 said:
That is sooo unbelievably cool.
I just started down the genetics path and kept seeing that two species can't be crossed and then i see this haha this is mad.
Good work bro aye

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veggie said:
Fascinating project! Great work so far. I'll be following along with much interest to see how it progresses.

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Judge Doom said:

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Guerrilla said:

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Judge Doom said:
I do not believe it is possible to cross dikaryotic strains in any compacity, including mixing grain together. What you need is monokaryons and then to confirm compatibility with clamp connections. Spontaneous dedikaryotization is very rare

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I'd be interested in a break down of your process. Are there any threads detailing what you went through? If not perhaps you would care to write one some day? A picture guide on mating spores / strain crossing with a scope would get a lot of attention.

You've greatly piqued my interest - I'm into the idea of getting a microscope more than ever now. Which one do you have?

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I will work on giving a visual outline in the future but for now I'll just give you a quick run down here. I germinated APE spores and isolated the monokaryons using my isolation technique, then my friend Yoshi Amano germinated Ps. natalensis spores and also isolated them using my technique. He handed me the natalensis monokaryons and I paired them with 16 of my cubensis monokaryons in some multiwell culture plates (48). I did a total of 144 pairings. After they had some time to colonize the wells I took small samples of the mycelium with a scalpel and checked the hyphae for clamp connections. Once I confirmed the presence of clamp connections I isolated the pairings in petris and then fruited them.



I'm glad you find it as fascinating as I do! I use a Amscope B120 compound microscope.

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I was starting to think you were Yoshi. I listened to all of the talks that he posted, cool stuff also, species is kind of a grey area. Different species can mate and produce fertile offspring. Savannah cats would be a good example of that, a hybrid of a serval and domestic cat. I read the F1,F2, and F3 males are often sterile though. So I don't see why nats wouldn't necessarily be there own species.

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SharaVabdas said:

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Psilosophy23 said:
That is sooo unbelievably cool.
I just started down the genetics path and kept seeing that two species can't be crossed and then i see this haha this is mad.
Good work bro aye

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Typically when two species can be so easily crossed, it indicates that they're closely related enough as to be considered the same species.

Since Nats can cross so readily with cubes, as has been demonstrated by Judge_Doom, it is likely that Natalensis should be considered a "subspecies" of cubensis, which there has been some ongoing debate about since Natalensis's original classification.

Note that there is a difference between a subspecies, a variety, and a cultivar. Subspecies indicates a different, yet compatible, genetic line that arose, specifically, due to geographical isolation from other populations of the organism. Among subspecies there can be naturally occurring varieties (the examples are usually the Bengal Tiger, being a subspecies of Tigers, and the various colors of Bengal tigers each being different varieties within that subspecies.)

A cultivar is, of course, a variety that was bred into existence by humans (it's actually at the same level of subspecies in the hierarchy, usually, but it's sort of its own thing.)

In this case I think Judge_Doom has proven that this species should be reclassified as a subspecies of Ps. Cubensis with their breeding work.

Some cross-species breeding can be done with transgenic (genetic modification) techniques, but that's not what's going on here.

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Tanavast said:
The fact they can can reproduce at all together I imagine

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Mr.Giggles said:
Even you and me have Neanderthal and Denisovan DNA, you may even have a little homo erectus in you lol dumb joke.

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