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Offlineburner
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Registered: 05/27/22
Posts: 52
Last seen: 7 months, 9 days
LC Submersion
    #27830390 - 06/21/22 01:31 PM (1 year, 7 months ago)

Ive made lots of LC lately and when i stir them up some seem to float at the top and others at the bottom like a big jellyfish... personally i think the bottom guys look better but id like opinion on these, should the mycelium sink to the bottom or float along the top to be generally healthy ? i stir them up fast to introduce O2. THANKS !


Edited by burner (06/21/22 03:36 PM)


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InvisibleChRnZN
Din of Doom
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Registered: 12/21/08
Posts: 6,265
Loc: ADK
Re: LC Submersion [Re: burner]
    #27830624 - 06/21/22 04:18 PM (1 year, 7 months ago)

Sunk mycelium is healthy and ready to inoculate. Stuff at the top should be stirred in unless you are checking for contamination.


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OfflinePBJ710
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Registered: 07/05/19
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Re: LC Submersion [Re: ChRnZN]
    #27838074 - 06/26/22 08:53 PM (1 year, 6 months ago)

Stuff that floats to the top is often a sign of mold IME.  Once the agar transfer is fully saturated, it should sink and stay submerged after a swirl.  Are any colonies growing on the glass at the top of the LC?


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