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SirPsycho
Purple Belt in Google-Fu



Registered: 01/01/20
Posts: 6,900
Loc: Rent free in your head
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Re: Ask Quick Questions, Get Quick Answers [Re: Grogu]
#28264824 - 04/06/23 08:29 AM (9 months, 18 days ago) |
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Quote:
Grogu said:
Quote:
SirPsycho said:
Quote:
Eugene Gesuale said: I’m no expert but it’s basically when the culture gets tired and stops producing well. After many many transfers and grows. I’m not sure how because it doesn’t make much sense in my mind. Like why can’t you just take a clone culture to keep it going. Why do you have to start back over from spore?
Because errors happen in the DNA transcription as cells divide, the more divisions the more errors and this eventually leads to cells not behaving as they should.
Like PBJ said, it's the same reason we experience more and more problems as we age.
So what's the workaround so the "package", in terms of Humans gets a full copy? Makes me wonder if it's natural to have things stay the same or get better and someone messed with genetics to make them degrade instead of at worse regenerate upon command from the body owner...

Lay off the drugs
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Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖
    "Mist your balls and fan your asshole" - Pandaskis, 2023
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Ingushkaya
Stranger


Registered: 07/03/21
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Re: Ask Quick Questions, Get Quick Answers [Re: SirPsycho]
#28264829 - 04/06/23 08:32 AM (9 months, 18 days ago) |
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Thank you for your response. I have a culture that i have had to transfer 10+ times and just now beginning to see growth that is promising and was afraid that it's age would have an effect for the culture. Glad to hear that this wasn't the case.
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SirPsycho
Purple Belt in Google-Fu



Registered: 01/01/20
Posts: 6,900
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Re: Ask Quick Questions, Get Quick Answers [Re: Mycodemus] 2
#28264831 - 04/06/23 08:34 AM (9 months, 18 days ago) |
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Quote:
Mycodemus said:
Quote:
SirPsycho said:
Quote:
Eugene Gesuale said: I’m no expert but it’s basically when the culture gets tired and stops producing well. After many many transfers and grows. I’m not sure how because it doesn’t make much sense in my mind. Like why can’t you just take a clone culture to keep it going. Why do you have to start back over from spore?
Because errors happen in the DNA transcription as cells divide, the more divisions the more errors and this eventually leads to cells not behaving as they should.
Like PBJ said, it's the same reason we experience more and more problems as we age.
Makes sense. I often take cuttings of my houseplants and wondered why it wasn’t the same, but I guess fungi are more like fauna in that respect.
Makes me wonder if you kept cloning a human clone if they would degrade… like in that movie Multiplicity haha
As I said in my other comment, senesence really isn't an issue for home cultivation. I'm not very well versed in plants but I'd imagine the same would sort of things happens there as well. Just that most plants tend to have a much longer life span.
If I were to guess, I'd imagine annuals would show signs of senesence much quicker than perennials if you kept taking cuttings and propagating that way
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Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖
    "Mist your balls and fan your asshole" - Pandaskis, 2023
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SirPsycho
Purple Belt in Google-Fu



Registered: 01/01/20
Posts: 6,900
Loc: Rent free in your head
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Re: Ask Quick Questions, Get Quick Answers [Re: Ingushkaya] 1
#28264836 - 04/06/23 08:35 AM (9 months, 18 days ago) |
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Quote:
Ingushkaya said: Thank you for your response. I have a culture that i have had to transfer 10+ times and just now beginning to see growth that is promising and was afraid that it's age would have an effect for the culture. Glad to hear that this wasn't the case. 
Nah, transfer to grain will have way more cellular divisions happen than 10 agar plates and people do multiple g2g "generations" without issue
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Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖
    "Mist your balls and fan your asshole" - Pandaskis, 2023
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trollettin_reyiz
Stranger



Registered: 01/27/22
Posts: 49
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Re: Ask Quick Questions, Get Quick Answers *DELETED* [Re: myc_ousin_vinny]
#28264844 - 04/06/23 08:37 AM (9 months, 18 days ago) |
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Post deleted by trollettin_reyiz
Reason for deletion: Wrong reply
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SirPsycho
Purple Belt in Google-Fu



Registered: 01/01/20
Posts: 6,900
Loc: Rent free in your head
Last seen: 2 seconds
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Yes, it is. Transfer on whenever you want.
Also, alcohol don't do shit to spores. If it did we wouldn't need to flame sterilize our tools to kill. Mold spores and bacterial endospores
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Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖
    "Mist your balls and fan your asshole" - Pandaskis, 2023
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Dirtnapz
Cake Kingdom



Registered: 02/22/23
Posts: 338
Loc: Lurkin yo Threads
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Re: Ask Quick Questions, Get Quick Answers [Re: Ingushkaya] 1
#28264849 - 04/06/23 08:39 AM (9 months, 18 days ago) |
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Morning everyone, should I consider transferring this before it starts to get closer to the edge?

Thanks in advance!
-------------------- Extinction is forever
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trollettin_reyiz
Stranger



Registered: 01/27/22
Posts: 49
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Re: Ask Quick Questions, Get Quick Answers [Re: Dirtnapz]
#28264850 - 04/06/23 08:40 AM (9 months, 18 days ago) |
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Alcohol touched my spores and I tried making do with what I have. It's been like a month. This is one of the best looking plates. You think I should clone from this? Is this mycelium?
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SirPsycho
Purple Belt in Google-Fu



Registered: 01/01/20
Posts: 6,900
Loc: Rent free in your head
Last seen: 2 seconds
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IQuote:
trollettin_reyiz said:

Alcohol touched my spores and I tried making do with what I have. It's been like a month. This is one of the best looking plates. You think I should clone from this? Is this mycelium?
Check two comments above your post, I responded before you deleted the other post
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Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖
    "Mist your balls and fan your asshole" - Pandaskis, 2023
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trollettin_reyiz
Stranger



Registered: 01/27/22
Posts: 49
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Re: Ask Quick Questions, Get Quick Answers [Re: SirPsycho] 2
#28264871 - 04/06/23 08:52 AM (9 months, 18 days ago) |
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Thank you so much SirPsycho
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ShroomNugget
Mycologist Wannabe



Registered: 02/10/23
Posts: 355
Last seen: 25 days, 21 hours
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A few simple questions if somebody could oblige:
I'm about to harvest my first PF cakes. How long should the second dunk take? 24 hours like the first? After the second dunk should I reroll in coir or just send straight to fruiting? I've heard both.
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johnukguy
Learning



Registered: 06/23/22
Posts: 2,423
Loc: Colorado
Last seen: 2 months, 23 days
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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
#28265060 - 04/06/23 10:47 AM (9 months, 17 days ago) |
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Quick question: Once pinning has started, can misting cause aborts?
-------------------- How to post pictures to shroomery TEK Shroomery Trusted Cultivator And Member YouTube Channels. “Evey Hammond: Who are you? V: Who? Who is but the form following the function of what and what I am is a man in a mask. Evey Hammond: Well I can see that. V: Of course you can. I'm not questioning your powers of observation I'm merely remarking upon the paradox of asking a masked man who he is”
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Screwup
Googles your dumb questions


Registered: 01/27/22
Posts: 6,296
Last seen: 28 minutes, 32 seconds
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Re: Ask Quick Questions, Get Quick Answers [Re: johnukguy] 1
#28265066 - 04/06/23 10:49 AM (9 months, 17 days ago) |
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Quote:
johnukguy said: Quick question: Once pinning has started, can misting cause aborts?
I’ve misted and will continue to do dumb shit like misting pins and havent had issues.
-------------------- 🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿 Help US help YOU TEK 2023 Dehydrator TEK
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Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 11,144
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Re: Ask Quick Questions, Get Quick Answers [Re: Screwup] 1
#28265070 - 04/06/23 10:51 AM (9 months, 17 days ago) |
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I’m not sure where the “don’t mist pins” thing comes from. I’ve misted pins on pretty much every shoebox and cake I’ve ever run and it has caused zero problems.
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Yahra
Meow


Registered: 03/06/23
Posts: 1,837
Loc: Earth
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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit] 1
#28265075 - 04/06/23 10:53 AM (9 months, 17 days ago) |
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I believe Pan pins are allergic to misting.
-------------------- Disobey
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Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 11,144
Loc: Stables
Last seen: 29 minutes, 18 seconds
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Re: Ask Quick Questions, Get Quick Answers [Re: Yahra]
#28265081 - 04/06/23 10:56 AM (9 months, 17 days ago) |
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Fair enough, I’ve never grown pans.
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SirPsycho
Purple Belt in Google-Fu



Registered: 01/01/20
Posts: 6,900
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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit] 1
#28265086 - 04/06/23 10:58 AM (9 months, 17 days ago) |
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Quote:
Smellyhobbit said: I’m not sure where the “don’t mist pins” thing comes from. I’ve misted pins on pretty much every shoebox and cake I’ve ever run and it has caused zero problems.
If you're using one of those big Zep spray bottles from Home Depot, the ones meant for cleaning products, that thing delivers a lot of force and pointing it directly at your fruit and misting from up close it's like poking them with a sharp stick.
I used to use them to spray my cat and it definitely bounces off him pretty forcefully
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Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖
    "Mist your balls and fan your asshole" - Pandaskis, 2023
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Yahra
Meow


Registered: 03/06/23
Posts: 1,837
Loc: Earth
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Re: Ask Quick Questions, Get Quick Answers [Re: SirPsycho] 2
#28265087 - 04/06/23 11:00 AM (9 months, 17 days ago) |
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-------------------- Disobey
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johnukguy
Learning



Registered: 06/23/22
Posts: 2,423
Loc: Colorado
Last seen: 2 months, 23 days
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Re: Ask Quick Questions, Get Quick Answers [Re: Yahra]
#28265099 - 04/06/23 11:06 AM (9 months, 17 days ago) |
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I've been misting more indirectly, from above and did wonder about this so thought it was worth asking. Thank you to all of you who responded. Much appreciated.
-------------------- How to post pictures to shroomery TEK Shroomery Trusted Cultivator And Member YouTube Channels. “Evey Hammond: Who are you? V: Who? Who is but the form following the function of what and what I am is a man in a mask. Evey Hammond: Well I can see that. V: Of course you can. I'm not questioning your powers of observation I'm merely remarking upon the paradox of asking a masked man who he is”
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Grogu


Registered: 07/02/22
Posts: 1,712
Last seen: 8 months, 11 days
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Re: Ask Quick Questions, Get Quick Answers [Re: SirPsycho]
#28265111 - 04/06/23 11:21 AM (9 months, 17 days ago) |
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Quote:
SirPsycho said:
Quote:
Grogu said:
Quote:
SirPsycho said:
Quote:
Eugene Gesuale said: I’m no expert but it’s basically when the culture gets tired and stops producing well. After many many transfers and grows. I’m not sure how because it doesn’t make much sense in my mind. Like why can’t you just take a clone culture to keep it going. Why do you have to start back over from spore?
Because errors happen in the DNA transcription as cells divide, the more divisions the more errors and this eventually leads to cells not behaving as they should.
Like PBJ said, it's the same reason we experience more and more problems as we age.
So what's the workaround so the "package", in terms of Humans gets a full copy? Makes me wonder if it's natural to have things stay the same or get better and someone messed with genetics to make them degrade instead of at worse regenerate upon command from the body owner...

Lay off the drugs
What drugs, if you didn't have the answer just say so...
People have been cured for unknown reasons having terrible afflictions, I was just curious since you sounded so in limitations instead of what's possible even if just in theory...
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