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ShroomNugget
Mycologist Wannabe



Registered: 02/10/23
Posts: 355
Last seen: 25 days, 21 hours
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Re: Ask Quick Questions, Get Quick Answers [Re: CocaineBuffet]
#28261281 - 04/03/23 09:40 PM (9 months, 20 days ago) |
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tholos said:
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ShroomNugget said:
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ShroomNugget said: Why are these guys so bruised up in this little patch? I assume it's a sign they are in need of something. More FAE? More water?
The bigger guy I circled on the other cake hasn't changed size in two days. Is it possible its done and I should harvest it? This is APE and they're still quite small. Standard sharpie in there for scale. Input would be much appreciated! My first grow, with APE none the less, but they seem to be turning out quite well.

Anybody out there with APE experience that can help a brutha out?
It is normal for APE caps to turn blue look up "Starry Night" APES or SNAPES to see an extremely blue cap phenotype -- nothing wrong with it!
I hope to get a hold of some SNAPEs soon from SS. They are so beautiful. 
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CocaineBuffet said:
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tholos said:
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ShroomNugget said:
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ShroomNugget said: Why are these guys so bruised up in this little patch? I assume it's a sign they are in need of something. More FAE? More water?
The bigger guy I circled on the other cake hasn't changed size in two days. Is it possible its done and I should harvest it? This is APE and they're still quite small. Standard sharpie in there for scale. Input would be much appreciated! My first grow, with APE none the less, but they seem to be turning out quite well.

Anybody out there with APE experience that can help a brutha out?
It is normal for APE caps to turn blue look up "Starry Night" APES or SNAPES to see an extremely blue cap phenotype -- nothing wrong with it!
The fruits will start to bruise as the cap breaks and cracks from getting too mature (personal preference).
Dark caps would usually be aborts if you are growing APE. I have not grown Starry Night so I don't have any first hand experience to draw from but I have grown plenty of APE.
Edit: To address the first post that I quoted I would say you are experiencing bruising from over misting. Those fruits are drenched.
I took the pic immediately after misting. Though it could be that I'm over misting, but I hear PE are thirsty mofos and there were times it seemed like they really grew between mistings. I thought they needed it but maybe I'm wrong . One thing is for sure, they need some misting or they dry out quick, within 6-8 hours. I was told being too dry was causing my mycelium to bruise prior to having pins as it was turning grey/blue. Since misting they've definitely been plumping up.
Thanks for the input fellas!
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Newshroom42
Stranger
Registered: 03/13/23
Posts: 222
Last seen: 3 months, 19 days
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Re: Ask Quick Questions, Get Quick Answers [Re: Grogu]
#28261322 - 04/03/23 10:45 PM (9 months, 20 days ago) |
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Ok perfect sounds good!
I plan on being as clean as possible anyways when doing the coier so I will be good on that. The tubs will be located in my closet with a curtain for a door so there will be FAE. I will have to run a heater as temps can get down in the house, but itβs on the other side of the rooms so should be good.
Only time I plan to pop the lid is either if it gets excessively dry or if the grits start pushing on the lid.
Hopefully that sounds like a decent setup!
Only question is the temp as I have the digital thermometer switch for the heater set to 70F, and thermometer at the level of the shoebox says 68. Hopefully that is a decent temp (currently doing some testing with that right now)
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Grogu


Registered: 07/02/22
Posts: 1,712
Last seen: 8 months, 11 days
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Re: Ask Quick Questions, Get Quick Answers [Re: Newshroom42]
#28261374 - 04/03/23 11:40 PM (9 months, 20 days ago) |
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Quote:
Newshroom42 said: Ok perfect sounds good!
I plan on being as clean as possible anyways when doing the coier so I will be good on that. The tubs will be located in my closet with a curtain for a door so there will be FAE. I will have to run a heater as temps can get down in the house, but itβs on the other side of the rooms so should be good.
Only time I plan to pop the lid is either if it gets excessively dry or if the grits start pushing on the lid.
Hopefully that sounds like a decent setup!
Only question is the temp as I have the digital thermometer switch for the heater set to 70F, and thermometer at the level of the shoebox says 68. Hopefully that is a decent temp (currently doing some testing with that right now)
I'm new but in my experience the magic I've grown like it above 65F so I guess your fine, and like some experts have said if your comfortable in the same environment as the mushrooms they will be also...
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trollettin_reyiz
Stranger



Registered: 01/27/22
Posts: 49
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Re: Ask Quick Questions, Get Quick Answers [Re: Grogu]
#28261520 - 04/04/23 04:57 AM (9 months, 20 days ago) |
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Hey guys! This is my original agar plate. I made more plates and tried to clone this one to others. I used a syringe tip and made it It looked like everything was going great at first.

But as you can see from the last plate, I see some kind of green coloring at most of these. What do you think this is? Could it still be mycelium? It kinda gives me the rhyzomorphic growth look, but like I told you the green color is giving me the heebie jeebies. Please someone tell me "Oh, that happens sometimes with the food coloring and all, it'll pass in a few days"
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DERRAYLD
Constructus

Registered: 05/13/02
Posts: 9,281
Loc: South Africa
Last seen: 1 hour, 14 minutes
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That's contaminated and no, it's not just something that happens with agar.
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Eyeofthunderer
Stranger

Registered: 07/11/20
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Last seen: 4 months, 28 days
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Re: Ask Quick Questions, Get Quick Answers [Re: DERRAYLD]
#28261572 - 04/04/23 06:20 AM (9 months, 20 days ago) |
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Ready to birth ?

Thanks
-------------------- It's the wait that kills you!
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Screwup
Googles your dumb questions


Registered: 01/27/22
Posts: 6,296
Last seen: 28 minutes, 52 seconds
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Re: Ask Quick Questions, Get Quick Answers [Re: Eyeofthunderer]
#28261574 - 04/04/23 06:22 AM (9 months, 20 days ago) |
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Doesnβt look great IMO but give it a few more days to see if it can colonize the uncolonized pieces.
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π° πΏ Help US help YOU TEK 2023 Dehydrator TEK
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trollettin_reyiz
Stranger



Registered: 01/27/22
Posts: 49
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Re: Ask Quick Questions, Get Quick Answers [Re: Eyeofthunderer]
#28261584 - 04/04/23 06:37 AM (9 months, 20 days ago) |
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Thanks, Derralyd.
Fine, back to square 1 then.
I have two big plates. The more-grown one is the first clone. Then I made the second one from the first. I'll inoculate more agar plates today. Which one do you think I should use? And if possible, which parts would be your favorite to clone?
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tholos
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Registered: 03/06/23
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Quote:
trollettin_reyiz said: Thanks, Derralyd.
Fine, back to square 1 then.
I have two big plates. The more-grown one is the first clone. Then I made the second one from the first. I'll inoculate more agar plates today. Which one do you think I should use? And if possible, which parts would be your favorite to clone?

First one looks sketchy, second one does not look promising either IMO
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PBJ710
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Registered: 07/05/19
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Re: Ask Quick Questions, Get Quick Answers [Re: tholos]
#28261596 - 04/04/23 06:51 AM (9 months, 20 days ago) |
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Both of those are heavily contaminated. Start a new project from a cleaner spore source - that syringe is likely bad since all of the growth is doing that.
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DelianSK13
Stranger

Registered: 03/29/23
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Last seen: 6 months, 20 days
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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
#28261617 - 04/04/23 07:14 AM (9 months, 20 days ago) |
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My cakes are fruiting in a water tub. How important is avoiding high temperature? Tomorrow it's going to be 80+ degrees out which means it will be 90+ in my 2nd floor apartment. I don't feel like putting in the window AC unit yet but I will if I have to.
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trollettin_reyiz
Stranger



Registered: 01/27/22
Posts: 49
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Re: Ask Quick Questions, Get Quick Answers [Re: PBJ710]
#28261622 - 04/04/23 07:17 AM (9 months, 20 days ago) |
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Quote:
PBJ710 said: Both of those are heavily contaminated. Start a new project from a cleaner spore source - that syringe is likely bad since all of the growth is doing that.
It wasn't a spore syringe. I just used a sterile syringe needle. I flame sterilized it frequently.
How can you tell they're contaminated? A few weeks ago I had posted them here and a few people said they're gathering tomentose. I'm not arguing, just asking what to watch out for. Cuz these look like good white stuff to me.
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myc_ousin_vinny
Keeping_It_Real



Registered: 04/29/20
Posts: 1,415
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Quote:
trollettin_reyiz said:
Quote:
PBJ710 said: Both of those are heavily contaminated. Start a new project from a cleaner spore source - that syringe is likely bad since all of the growth is doing that.
It wasn't a spore syringe. I just used a sterile syringe needle. I flame sterilized it frequently.
How can you tell they're contaminated? A few weeks ago I had posted them here and a few people said they're gathering tomentose. I'm not arguing, just asking what to watch out for. Cuz these look like good white stuff to me. 
So it was a print?
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trollettin_reyiz
Stranger



Registered: 01/27/22
Posts: 49
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I need help, guys. From the above conversation, you can see that people said my big plates might be contam.
Since I'm in a country that is very hard to get spores, I need your advice BADLY.
I'll tell you guys what I have.
1- Weak Spores I received a spore print. I tried to put it inside the SAB after cleaning with alcohol. It turned out that the plastic bag had holes, and the alcohol went in and touched all the spores. I tried to salvage what I can with these agar plates. Do you think I can clone from any of these?

2- Grain on Agar This grain was inoculated with some of those things I had on the bigger plates a few posts ago. Looking from the under parts, there's some dark formation.

3- Cardboard on Agar from Dried Fruit I had put some dried fruits on cardboard. Then I put some white fuzzy pieces on agar. Do you think the white parts are worth a shot? Or just cobweb mold?

4- Dried Fruits and Caps I have some dried fruits and caps. Do you think I could clone from these? I could get these into my SAB and touch the gills with a scalpel and throw them on the agar plates.
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PBJ710
Strangler


Registered: 07/05/19
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Was that a josex poke then? Your original agar has splotchy pattern with no indicators of radial growth from the inoculation point and the T2s(?) appear to just be a more isolated culture of the mold.
This thread will show and describe way better than I can, but the dense growth in the middle with hazy growth at the margins, and foamy looking growth, swiss cheese tendrils & the wet margins are also consistent with contams.
How it should & shouldn't look - NEW CULTIVATORS GUIDE - go to the mycelium on agar section.
You may be better off starting your own thread to keep track of everything since this has gone way beyond quick questions and answers.
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trollettin_reyiz
Stranger



Registered: 01/27/22
Posts: 49
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Quote:
myc_ousin_vinny said:
Quote:
trollettin_reyiz said:
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PBJ710 said: Both of those are heavily contaminated. Start a new project from a cleaner spore source - that syringe is likely bad since all of the growth is doing that.
It wasn't a spore syringe. I just used a sterile syringe needle. I flame sterilized it frequently.
How can you tell they're contaminated? A few weeks ago I had posted them here and a few people said they're gathering tomentose. I'm not arguing, just asking what to watch out for. Cuz these look like good white stuff to me. 
So it was a print?

Here is their mother. I had some dried fruits, and I touched the gills with the scalpel to throw some spores on the agars. One of them resulted with this agar plate. People said it looked clean enough, and that it was mycelium, however tomentose it is. They said it was probably multi-spore, hence the ballish growth. I cloned this little dude into more agars. Two of the plates, I've already showed you in the first message I showed today. Here's another plate I cloned from that little ball.

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trollettin_reyiz
Stranger



Registered: 01/27/22
Posts: 49
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Re: Ask Quick Questions, Get Quick Answers [Re: PBJ710]
#28261674 - 04/04/23 08:06 AM (9 months, 20 days ago) |
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Quote:
PBJ710 said: Was that a josex poke then? Your original agar has splotchy pattern with no indicators of radial growth from the inoculation point and the T2s(?) appear to just be a more isolated culture of the mold.
This thread will show and describe way better than I can, but the dense growth in the middle with hazy growth at the margins, and foamy looking growth, swiss cheese tendrils & the wet margins are also consistent with contams.
How it should & shouldn't look - NEW CULTIVATORS GUIDE - go to the mycelium on agar section.
You may be better off starting your own thread to keep track of everything since this has gone way beyond quick questions and answers.
I had to research the josex poke, but yes, it looks similar. I flame sterilized a needle tip, red hot, then scraped the white stuff a bit and touched it on the agar. I made sure that the syringe tip wasn't too hot before touching anything.
And you're right about a different thread, but it wouldn't get the same attention, this is a popular thread and I need all the help I can get, asap 
Promise I won't post anything long from now on, just answers to you fellow shroomers
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machinym
hmm


Registered: 03/10/23
Posts: 68
Last seen: 2 days, 15 hours
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Why do noobies keep doing orbeez tek? I've never seen a good canopy from it.
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Screwup
Googles your dumb questions


Registered: 01/27/22
Posts: 6,296
Last seen: 28 minutes, 52 seconds
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Re: Ask Quick Questions, Get Quick Answers [Re: machinym] 2
#28261684 - 04/04/23 08:19 AM (9 months, 20 days ago) |
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Because Reddit is a hell of a drug
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π
π° πΏ Help US help YOU TEK 2023 Dehydrator TEK
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LSDCubed
Finally I understand peace



Registered: 06/10/21
Posts: 47
Loc: US
Last seen: 5 months, 18 days
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What's happening here [Re: Screwup]
#28261710 - 04/04/23 08:45 AM (9 months, 20 days ago) |
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What are these blobs? Supposed to be B+ but on a previous syringe from this vendor, I got albinos (AA+?) from what was supposed to be Ban Hua Thanon.
Agar to Grain to g2g to cvg. Everything looked and smelled good but I'm a noob. I didn't use casing on this mono just for the sake of experimenting. Other monos have good growth, no blobs (yet).
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