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OfflineCocaineBuffet
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Re: Ask Quick Questions, Get Quick Answers [Re: Bobgas] * 7
    #28259807 - 04/02/23 09:18 PM (9 months, 21 days ago)

The clone is not stabilized enough so you are still getting mixed results. Keep cloning the ones you want that have the same trait and you will be able to get more consistency.


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OfflineBobgas
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Re: Ask Quick Questions, Get Quick Answers [Re: CocaineBuffet] * 2
    #28259816 - 04/02/23 09:24 PM (9 months, 21 days ago)

Quote:

CocaineBuffet said:
The clone is not stabilized enough so you are still getting mixed results. Keep cloning the ones you want that have the same trait and you will be able to get more consistency.




I completely misunderstood the concept of working with clones.  Thank you.  That’s very helpful.


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OfflineCocaineBuffet
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Re: Ask Quick Questions, Get Quick Answers [Re: Bobgas] * 1
    #28259823 - 04/02/23 09:27 PM (9 months, 21 days ago)

I mean that is my assumption based on my experience working with clone plates. I expected them to be a CLONE of the same fruit but it hasn't been my experience so I figured that is probably what it is.

I wouldn't say contamination because there is no contamination present to my eye.


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OfflineEugene Gesuale
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Re: Ask Quick Questions, Get Quick Answers [Re: CocaineBuffet] * 1
    #28260005 - 04/03/23 04:30 AM (9 months, 21 days ago)

Iirc when you take a tissue biopsy to agar you can have as many as 9 sectors on a plate.


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OfflineHorsewithnoname
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Re: Ask Quick Questions, Get Quick Answers [Re: Eugene Gesuale] * 1
    #28260029 - 04/03/23 05:26 AM (9 months, 21 days ago)

Why isn't honey agar more popular? Does it work? I'm asking because given because the thing I had I thought was malt extract is actually malted barley flour (which explains the extreme levels of sediment I was getting).

I'm wondering if I should try making homemade malt extract from the flour or just switch to something else.


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Invisiblemilkboy
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Re: Ask Quick Questions, Get Quick Answers [Re: Horsewithnoname] * 1
    #28260031 - 04/03/23 05:28 AM (9 months, 21 days ago)

Tons of agar recipes "work" but honeys expensive and my mushrooms dont have taste buds


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OfflineNFLProof
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Re: Ask Quick Questions, Get Quick Answers [Re: Horsewithnoname]
    #28260033 - 04/03/23 05:34 AM (9 months, 21 days ago)

I'm not sure which tek you're using but if boiling before pc'ing, could you strain it after the microwave step?


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OfflineHorsewithnoname
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Re: Ask Quick Questions, Get Quick Answers [Re: NFLProof]
    #28260039 - 04/03/23 05:55 AM (9 months, 21 days ago)

Quote:

milkboy said:
Tons of agar recipes "work" but honeys expensive and my mushrooms dont have taste buds




Funnily, getting actual malt extract where I leave seems more expensive than just using honey (which I pretty much always have at home).

So I'm wondering if there are any downsides to honey I'm unaware of.

Quote:

NFLProof said:
I'm not sure which tek you're using but if boiling before pc'ing, could you strain it after the microwave step?




Just a regular LME 2% recipe. It just turns out that what I'm using isn't actually malt extract, it's the milled malted barley grains that can be used to make malt extract.

For my last agar batch I filtered the water+flour mix but was still left with a bunch of sediment sadly. I'm currently looking into how malt extract is made to see if I can reproduce something like that for agar.

But I'm also wondering if it just isn't worth the trouble and I should use something else more easily available here (like honey).


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OfflineHorsewithnoname
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Re: Ask Quick Questions, Get Quick Answers [Re: Horsewithnoname] * 1
    #28260045 - 04/03/23 06:07 AM (9 months, 21 days ago)

On that note, wtf are potato flakes? Is it just another name for that dry mashed potatoes mix, like to make puree by adding water/milk? Or is it something else?


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OfflineGrogu
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Re: Ask Quick Questions, Get Quick Answers [Re: Horsewithnoname] * 2
    #28260057 - 04/03/23 06:18 AM (9 months, 21 days ago)

Quote:

Horsewithnoname said:
On that note, wtf are potato flakes? Is it just another name for that dry mashed potatoes mix, like to make puree by adding water/milk? Or is it something else?






:pointmade:


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OfflineHorsewithnoname
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Re: Ask Quick Questions, Get Quick Answers [Re: Grogu]
    #28260070 - 04/03/23 06:48 AM (9 months, 21 days ago)

How to make malt extract out of malted barley flour according to ChatGPT (GPT-4)

Quote:



Malted Barley Extract: Sediment-Free

Model: GPT-4

How do I turn malted barley flour into malt extract that won't produce much sediment when mixed with water?

To make a malt extract from malted barley flour with minimal sediment, you can follow these steps:
  • Measure your malted barley flour: For this process, you can use a ratio of 1:4 (1 part malted barley flour to 4 parts water). The exact amount of malt extract you need depends on your recipe, but these ratios will help you scale up or down.

  • Mix with cold water: In a large pot, slowly add the malted barley flour to cold water while stirring constantly. Make sure there are no lumps or clumps. Using cold water will help reduce the chances of sediment forming.

  • Heat the mixture: Gradually heat the mixture over low to medium heat, stirring constantly to ensure even heating and to prevent the flour from settling at the bottom of the pot. Do not allow the mixture to boil, as this can cause sediment to form.

  • Maintain the temperature: Once the mixture reaches a temperature of 150-160°F (65-70°C), maintain this temperature for 30-60 minutes. This will allow the enzymes in the malted barley flour to break down the starches into fermentable sugars. Stir the mixture occasionally to prevent sediment from forming.

  • Cool the mixture: After the starch conversion is complete, remove the pot from the heat and allow the mixture to cool down to room temperature. The cooler temperature will help any remaining sediment to settle at the bottom of the pot.

  • Filter the liquid: To remove the sediment, carefully pour the liquid through a fine mesh strainer, cheesecloth, or coffee filter placed over a clean container. This will help you separate the liquid malt extract from any remaining sediment. Be patient during this step, as it may take some time for the liquid to pass through the filter.

  • Optional - reduce the liquid: If you want a more concentrated malt extract, you can return the filtered liquid to the pot and gently simmer it over low heat until it reaches your desired consistency. Be sure to stir frequently to prevent scorching.

  • Cool and store: Once you have achieved the desired consistency, allow the malt extract to cool to room temperature. Then, transfer it to an airtight container for storage. You can store the malt extract in the refrigerator for up to several weeks or freeze it for longer-term storage.

  • When using your homemade malt extract, remember to mix it well with water to ensure proper dissolution and minimal sediment formation. It's also a good idea to use a fine mesh strainer or cheesecloth when transferring the mixture to your fermenting vessel to catch any remaining sediment.

    By following these steps, you can create a malt extract from malted barley flour that produces minimal sediment when mixed with water, making it a suitable ingredient for brewing or other culinary applications.





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    InvisibleKeyMaker
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    Re: Ask Quick Questions, Get Quick Answers [Re: Horsewithnoname]
        #28260075 - 04/03/23 07:02 AM (9 months, 21 days ago)

    I germed some plates about a month ago, did a few transfers.  I have a few plates I was going to send to grain this week, but I noticed one of the germ plates I kept has two little knots forming.  I'm not trying to grow a lot of mushrooms at the moment, as I have plenty.  I'm trying to get the best genetics for a future grow.  So the question is, should I just wait til a pin forms on that germ plate and transfer it, instead of growing fruits to clone?


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    OfflineNFLProof
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    Re: Ask Quick Questions, Get Quick Answers [Re: KeyMaker] * 1
        #28260092 - 04/03/23 07:18 AM (9 months, 21 days ago)

    Why not both?


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    InvisibleKeyMaker
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    Re: Ask Quick Questions, Get Quick Answers [Re: NFLProof]
        #28260101 - 04/03/23 07:25 AM (9 months, 21 days ago)

    I could certainly do both, but if one is better than the other, I'd like to know.  I'm trying to limit the amount of space I use for this hobby, as well as the materials I go through.  If I correctly understand how cloning works, can I skip the time it would take for me to grow all these shrooms I don't need, just to have something to clone?


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    Offlineherbstation
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    Re: Ask Quick Questions, Get Quick Answers [Re: Horsewithnoname] * 1
        #28260105 - 04/03/23 07:26 AM (9 months, 21 days ago)

    Malt extract is just the sugars and some flavor from sprouted barley when you extract it via boiling. AKA yeah it's pretty easy to make your own extract and if you add hops and yeast your extract becomes a wort which eventually ferments into a beer. It makes sense that we use it for mushrooms cause it's essentially yeast food.

    If you dry the extract it's called Dry Malt Extract, if you reduce it into a syrup it's Liquid Malt Extract, and if you toast the barley before you boil it then you get Dark Malt Extract, which has less sugar and more FLAVA.

    Dunno the process for making it with flour. Malting = sprouting so if you have some oats on hand you could always just malt them and then extract those! I think if you're going to go that far you may as well use honey though. It would be a fun project but not a time saver.

    I use just grain water with agar and it does great!

    Edit: I also see ChatGPTs recipe. You have to simmer grains at 150 for beer or they will release off flavors. I think for agar you can boil it, you're not gonna be drinking the end result.

    Cheers


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    Edited by herbstation (04/03/23 07:30 AM)


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    OfflineDERRAYLD
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    Re: Ask Quick Questions, Get Quick Answers [Re: KeyMaker] * 1
        #28260108 - 04/03/23 07:28 AM (9 months, 21 days ago)

    Quote:

    KeyMaker said:
    I could certainly do both, but if one is better than the other, I'd like to know.  I'm trying to limit the amount of space I use for this hobby, as well as the materials I go through.  If I correctly understand how cloning works, can I skip the time it would take for me to grow all these shrooms I don't need, just to have something to clone?




    Go for a clone not a plin in that case because you won't know what the plin will express until much later after g2g which could be a waste of time.
    I've had this happen before after using a plin, the result wasn't favorable so I went back to clone.


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    OfflineHorsewithnoname
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    Re: Ask Quick Questions, Get Quick Answers [Re: Grogu] * 1
        #28260111 - 04/03/23 07:29 AM (9 months, 21 days ago)

    Quote:

    Grogu said:
    Quote:

    Horsewithnoname said:
    On that note, wtf are potato flakes? Is it just another name for that dry mashed potatoes mix, like to make puree by adding water/milk? Or is it something else?






    :pointmade:




    :mindexpanding:


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    InvisibleKeyMaker
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    Re: Ask Quick Questions, Get Quick Answers [Re: DERRAYLD]
        #28260116 - 04/03/23 07:35 AM (9 months, 21 days ago)

    Quote:

    DERRAYLD said:
    Quote:

    KeyMaker said:
    I could certainly do both, but if one is better than the other, I'd like to know.  I'm trying to limit the amount of space I use for this hobby, as well as the materials I go through.  If I correctly understand how cloning works, can I skip the time it would take for me to grow all these shrooms I don't need, just to have something to clone?




    Go for a clone not a plin in that case because you won't know what the plin will express until much later after g2g which could be a waste of time.
    I've had this happen before after using a plin, the result wasn't favorable so I went back to clone.




    Thanks.  In what situation is it favorable to clone a pin from a plate?  I've seen this advised in different circumstances.


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    OfflineSan Pedro GirlS
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    Re: Ask Quick Questions, Get Quick Answers [Re: Horsewithnoname] * 2
        #28260124 - 04/03/23 07:44 AM (9 months, 21 days ago)


    Quote:

    Horsewithnoname said:
    Quote:

    milkboy said:
    Tons of agar recipes "work" but honeys expensive and my mushrooms dont have taste buds




    Funnily, getting actual malt extract where I leave seems more expensive than just using honey (which I pretty much always have at home).

    So I'm wondering if there are any downsides to honey I'm unaware of.

    Quote:

    NFLProof said:
    I'm not sure which tek you're using but if boiling before pc'ing, could you strain it after the microwave step?




    Just a regular LME 2% recipe. It just turns out that what I'm using isn't actually malt extract, it's the milled malted barley grains that can be used to make malt extract.

    For my last agar batch I filtered the water+flour mix but was still left with a bunch of sediment sadly. I'm currently looking into how malt extract is made to see if I can reproduce something like that for agar.

    But I'm also wondering if it just isn't worth the trouble and I should use something else more easily available here (like honey).



    One downside other than cost is that honey contains fructose, which will caramelize in a PC. Similar to honey, but with a much better nutrient profile is molasses if you just like experimenting.


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    OfflineSan Pedro GirlS
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    Re: Ask Quick Questions, Get Quick Answers [Re: KeyMaker]
        #28260138 - 04/03/23 07:54 AM (9 months, 21 days ago)

    Quote:

    KeyMaker said:
    Quote:

    DERRAYLD said:
    Quote:

    KeyMaker said:
    I could certainly do both, but if one is better than the other, I'd like to know.  I'm trying to limit the amount of space I use for this hobby, as well as the materials I go through.  If I correctly understand how cloning works, can I skip the time it would take for me to grow all these shrooms I don't need, just to have something to clone?




    Go for a clone not a plin in that case because you won't know what the plin will express until much later after g2g which could be a waste of time.
    I've had this happen before after using a plin, the result wasn't favorable so I went back to clone.




    Thanks.  In what situation is it favorable to clone a pin from a plate?  I've seen this advised in different circumstances.



    I grow almost exclusively from plate pins. The plate pin has already proven to be strong genetics because it beat out all other genetics on the plate.

    However, some plate pins just suck and end up making a bunch of tiny, short fruits. Yes, it’s a clone that quickly limits genetics since most of mine come from my first transfer plates, but you don’t really know what you’ll get until you grow it out.


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