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OnlineSpicolis_Dad
Gaian Warrior
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Registered: 08/24/20
Posts: 75
Last seen: 6 seconds
Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #27965744 - 09/24/22 09:36 AM (1 year, 5 months ago)

No worries, thank you for the quick reply... leave it to me to find something Nats can't deal with:crazy:

Learning is learning.  May I assume that if I can see the color, it has sporulated and that anything in the tub should be tossed?

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OfflineRusty2096
rah rah raw in Lady gaga
I'm a teapot User Gallery


Registered: 08/23/22
Posts: 4,946
Loc: 🌌
Last seen: 23 days, 10 hours
Re: Ask Quick Questions, Get Quick Answers [Re: Spicolis_Dad] * 1
    #27965767 - 09/24/22 09:47 AM (1 year, 5 months ago)

Quote:

Spicolis_Dad said:
No worries, thank you for the quick reply... leave it to me to find something Nats can't deal with:crazy:

Learning is learning. May I assume that if I can see the color, it has sporulated and that anything in the tub should be tossed?




Yes. Compost bin ftw.

Also, your positive attitude is commendable. :super:

Do not :whiteflag:.

Your next project will be just fine, the universe told me so.

Edit: Also, your attitude just earned you your first rating :dancer:


--------------------
Currently looking for nothing. You guys who sent me stuff are straight up awesome!. :mushroom2:

We don't own things - things own us.

Semi-solid liquid culture (SSLC)

Edited by Rusty2096 (09/24/22 09:49 AM)

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OnlineSpicolis_Dad
Gaian Warrior
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Registered: 08/24/20
Posts: 75
Last seen: 6 seconds
Re: Ask Quick Questions, Get Quick Answers [Re: Rusty2096]
    #27965778 - 09/24/22 09:56 AM (1 year, 5 months ago)

Quote:

Rusty2096 said:
Quote:

Spicolis_Dad said:
No worries, thank you for the quick reply... leave it to me to find something Nats can't deal with:crazy:

Learning is learning. May I assume that if I can see the color, it has sporulated and that anything in the tub should be tossed?




Yes. Compost bin ftw.

Also, your positive attitude is commendable. :super:

Do not :whiteflag:.

Your next project will be just fine, the universe told me so.

Edit: Also, your attitude just earned you your first rating :dancer:




Thanks man, I appreciate it. Thanks for the positive vibe too!

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OfflineScrewup
Googles your dumb questions
Trans-female

Registered: 01/27/22
Posts: 6,339
Last seen: 2 hours, 18 minutes
Re: Ask Quick Questions, Get Quick Answers [Re: Spicolis_Dad]
    #27965837 - 09/24/22 10:29 AM (1 year, 5 months ago)

Yeah that shits fucked. If it ain’t trich it’s some other mold or bacteria IMO.

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Invisiblemilkboy
Child
I'm a teapot User Gallery


Registered: 05/01/21
Posts: 2,318
Re: Ask Quick Questions, Get Quick Answers [Re: Screwup]
    #27966219 - 09/24/22 03:25 PM (1 year, 5 months ago)

Do any of you nerds do agar to agar transfers with a sterile swab ?


--------------------
Gummies by milkboy
Milkboys Public Display of Affection

"Vires acquirit eundo"


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OfflineNichrome
I'm a torso!
I'm a teapot User Gallery

Registered: 12/17/18
Posts: 6,728
Loc: Zone 5
Last seen: 6 hours, 8 minutes
Trusted Cultivator
Re: Ask Quick Questions, Get Quick Answers [Re: milkboy]
    #27966225 - 09/24/22 03:29 PM (1 year, 5 months ago)

No but I wouldn't slight you at all for doing so. Whatever you have that will work is my motto.


--------------------
Once you've got something figured out completely, you're stupid.

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OfflineGrind365
AlwaysGrinding

Registered: 08/07/22
Posts: 448
Last seen: 1 year, 1 month
Re: Ask Quick Questions, Get Quick Answers [Re: milkboy]
    #27966239 - 09/24/22 03:37 PM (1 year, 5 months ago)

Quote:

milkboy said:
Do any of you nerds do agar to agar transfers with a sterile swab ?



Milkboy. I'm a noob but as a experiment I sterilized cut off swab ends clamped into hemostat forceps. I then used the sterile tools to swab clean agar cultures before dropping the swab ends into grain jars for inoculation.

Performance was very poor most did nothing 1 jar was successful.
My guess is the mycelium dried out before getting a chance to jump off.

I know this isnt what you asked but thought I'd share my experience.


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InvisibleJackFrost67

Registered: 08/27/22
Posts: 61
Re: Ask Quick Questions, Get Quick Answers [Re: Grind365]
    #27966604 - 09/24/22 08:30 PM (1 year, 5 months ago)

c

Edited by JackFrost67 (10/20/22 06:26 PM)

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OfflineRusty2096
rah rah raw in Lady gaga
I'm a teapot User Gallery


Registered: 08/23/22
Posts: 4,946
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Re: Ask Quick Questions, Get Quick Answers [Re: JackFrost67]
    #27966655 - 09/24/22 09:27 PM (1 year, 5 months ago)

Quote:

itskater said:
I'm getting some liquid culture soon but it might be a week or so. I have a 3.5lb bag of grain spawn in a cooler bag with ice packs but I also just purchased a pressure cooker that will cook at 15 psi. Should I re sterilize before inoculating the bag?

If so should it be at 15 psi for an hour and a half with the whole bag and all put in (raised so its not directly on the bottom)?

Thanks!






That's the picture of an insta pot, not a PC. The difference is that you have to basically "trust" the insta pot to really be at 15 PSI and have no way to know for sure. Where with a PC you do know. I know 2 people who owned an insta pot intended for sterilization and both returned it within a few uses.

I'm not saying it's not going to work or anything, but if it's not too late already you might want to consider a PC.

Whatever you do, best of luck to you :super:


--------------------
Currently looking for nothing. You guys who sent me stuff are straight up awesome!. :mushroom2:

We don't own things - things own us.

Semi-solid liquid culture (SSLC)

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InvisibleJackFrost67

Registered: 08/27/22
Posts: 61
Re: Ask Quick Questions, Get Quick Answers [Re: Rusty2096]
    #27966733 - 09/24/22 10:32 PM (1 year, 5 months ago)

c

Edited by JackFrost67 (10/20/22 06:25 PM)

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InvisibleStipe-n CapMDiscord
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Registered: 08/04/12
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Re: Ask Quick Questions, Get Quick Answers [Re: JackFrost67] * 2
    #27966734 - 09/24/22 10:34 PM (1 year, 5 months ago)

:whathesaid:

You can't sterilize grains in an instant pot, not suitable as an autoclave.

More like this:




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OfflineRusty2096
rah rah raw in Lady gaga
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Registered: 08/23/22
Posts: 4,946
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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #27966756 - 09/24/22 11:04 PM (1 year, 5 months ago)

How do you guys calculate your spawn:bulk ratio.

For me, let's say I want to do 1:3, I will mesure mycoquarts (let's call that 600ml) for both sides. So 1 mycoquart spawn for 1800ml bulk (1:3)

I know some/many will calculate in mycoquarts for spawn but full quarts (950ml or so) for spawn. So those people would call 600ml spawn and 2850ml bulk a 1:3 ratio.

The difference in considerable. I know in the end it doesn't matter so much and is up to everyone's experience and preference (the 1:3 ratio here was purely given as an example) but still I'm curious if you guys count mycoquart:mycoquart or mycoquart:fullquart when you talk about your ratio.


--------------------
Currently looking for nothing. You guys who sent me stuff are straight up awesome!. :mushroom2:

We don't own things - things own us.

Semi-solid liquid culture (SSLC)

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InvisibleCowsPoopShrooms
StillUphill


Registered: 06/08/22
Posts: 505
Re: Ask Quick Questions, Get Quick Answers [Re: Rusty2096]
    #27966760 - 09/24/22 11:08 PM (1 year, 5 months ago)

mycoquart

Edited by CowsPoopShrooms (10/14/22 05:39 PM)

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OfflineVibetyme
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Registered: 06/08/21
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Re: Ask Quick Questions, Get Quick Answers [Re: JackFrost67] * 1
    #27966771 - 09/24/22 11:30 PM (1 year, 5 months ago)

Quote:

itskater said:
I'm getting some liquid culture soon but it might be a week or so. I have a 3.5lb bag of grain spawn in a cooler bag with ice packs but I also just purchased a pressure cooker that will cook at 15 psi. Should I re sterilize before inoculating the bag?

If so should it be at 15 psi for an hour and a half with the whole bag and all put in (raised so its not directly on the bottom)?

Thanks!






I'd look up the specs on your Cuisinart model but I'm betting it might reach 15psi but then the heat will shut off and it will actually cook in the 12-13psi range for the bulk of the time.  That is what my instant pot does anyway.

I do all my grain sterilization in an instant pot and it is small but works well none the less. Is it as good as a pc? No it's probably not.  But it is set and forget most definitely.  I just have to let em go 3.5 hrs but no worries cuz there is no babysitting involved.

Check this chart out on sterilization times and psi you need to reach.


RR did his grain in a steam barrel outside for 12 hrs on 1-2psi. It just takes more time the lower you go.


--------------------
Vibetyme's LAGM 2.022

Vibetyme's LAGM_23

Vibetyme's LAGM_24

                 

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InvisibleJackFrost67

Registered: 08/27/22
Posts: 61
Re: Ask Quick Questions, Get Quick Answers [Re: Vibetyme]
    #27966812 - 09/25/22 12:21 AM (1 year, 5 months ago)

v

Edited by JackFrost67 (10/20/22 06:23 PM)

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OfflineGrogu
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Registered: 07/02/22
Posts: 1,712
Last seen: 9 months, 13 days
Re: Ask Quick Questions, Get Quick Answers [Re: Vibetyme]
    #27966866 - 09/25/22 01:31 AM (1 year, 5 months ago)

Quote:

Vibetyme said:
Quote:

itskater said:
I'm getting some liquid culture soon but it might be a week or so. I have a 3.5lb bag of grain spawn in a cooler bag with ice packs but I also just purchased a pressure cooker that will cook at 15 psi. Should I re sterilize before inoculating the bag?

If so should it be at 15 psi for an hour and a half with the whole bag and all put in (raised so its not directly on the bottom)?

Thanks!






I'd look up the specs on your Cuisinart model but I'm betting it might reach 15psi but then the heat will shut off and it will actually cook in the 12-13psi range for the bulk of the time.  That is what my instant pot does anyway.

I do all my grain sterilization in an instant pot and it is small but works well none the less. Is it as good as a pc? No it's probably not.  But it is set and forget most definitely.  I just have to let em go 3.5 hrs but no worries cuz there is no babysitting involved.

Check this chart out on sterilization times and psi you need to reach.


RR did his grain in a steam barrel outside for 12 hrs on 1-2psi. It just takes more time the lower you go.




How could I see my 4-4qt or 4 bags/16 qts in that chart? I know to do it 3.5hrs but not sure how to convert to big bags?

:shrug:


--------------------


Mushroom Cultivation Techniques...

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Offlinerumfor69
Bodhicitta Cultivator
Male


Registered: 08/05/11
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Last seen: 23 hours, 5 minutes
Re: Ask Quick Questions, Get Quick Answers [Re: Stipe-n Cap]
    #27966907 - 09/25/22 03:07 AM (1 year, 5 months ago)

Quote:

p9hu7 said:






I have 2 of those cadco electric hot plates. They work great and they just keep going and going tons of hours of use outta them so far.


--------------------

Links For Beginners And Beyond:mspoil:

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InvisibleStipe-n CapMDiscord
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Registered: 08/04/12
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Re: Ask Quick Questions, Get Quick Answers [Re: rumfor69] * 6
    #27967028 - 09/25/22 07:11 AM (1 year, 5 months ago)

So, just fyi guys....that chart is useless.

Instant pots are not appropriate for grain; maybe you can get away with liquid media? Still though...

Instant pot's are not appropriate equipment for microbiology, period. Do not buy an instant pot hoping that it will work as an autoclave. You will not find an instant pot in a hospital, dentists office  tattoo parlor, mycology/microbiology lab  etc.

Why?

Because they're for making soup, yogurt, etc. They're are not suitable for the purpose of effectively destroying thermophilic bacterial endospores.

Edited by Stipe-n Cap (09/25/22 07:19 AM)

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OfflineFarm3r
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Registered: 05/04/21
Posts: 535
Loc: River of the West Flag
Last seen: 1 year, 3 months
Re: Ask Quick Questions, Get Quick Answers [Re: Stipe-n Cap]
    #27967039 - 09/25/22 07:20 AM (1 year, 5 months ago)

I wonder what happened to AKA and Chron. They were using them and both were TC and Chron was MOD. So there is people using them.

https://www.shroomery.org/forums/showflat.php/Number/26423474/fpart/1/vc/1

If it stays at 15psi it will.

Edited by Farm3r (09/25/22 07:20 AM)

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InvisibleStipe-n CapMDiscord
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Registered: 08/04/12
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Loc: Canada Flag
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Re: Ask Quick Questions, Get Quick Answers [Re: Farm3r]
    #27967042 - 09/25/22 07:21 AM (1 year, 5 months ago)

Like I said, liquids.

They're not using them for grain sterilization.

You again, eh, :lol:

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