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EniQma
Registered: 11/28/23
Posts: 470
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Re: Ask Quick Questions, Get Quick Answers [Re: Luminous7]
#28631673 - 01/23/24 03:33 AM (4 days, 19 hours ago) |
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QQ: From where shall I take my tissue
Edited by EniQma (01/23/24 03:33 AM)
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SwabMarley
Twisted Metal Head



Registered: 12/07/20
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Loc: Drunken Stupor
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Re: Ask Quick Questions, Get Quick Answers [Re: EniQma]
#28631679 - 01/23/24 03:45 AM (4 days, 19 hours ago) |
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For cloning? I’d take some from the big boy on his own and some from the biggest one of the small cluster in the back centre of the tub.
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EniQma
Registered: 11/28/23
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Re: Ask Quick Questions, Get Quick Answers [Re: SwabMarley]
#28631683 - 01/23/24 03:55 AM (4 days, 19 hours ago) |
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Would you send them each on their own or mix spawn in same tub later
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SwabMarley
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Re: Ask Quick Questions, Get Quick Answers [Re: EniQma]
#28631739 - 01/23/24 06:15 AM (4 days, 17 hours ago) |
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I’d take the 2 separate clones and send them each to a jar and see which one performs best.
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psilocybenjamin
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Re: Ask Quick Questions, Get Quick Answers [Re: Luminous7]
#28631944 - 01/23/24 09:46 AM (4 days, 13 hours ago) |
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Quote:
Luminous7 said:
Quote:
psilocybenjamin said: This is my first grow. Just put into fruiting conditions a couple days ago. It’s looking kinda fuzzy even though the fan is always on.
What do you guys think? Any feedback at all would be appreciated.


It looks ok , starting to show knots. But that set up is unnecessary dawg
The myc will consume the metal trays putting holes in it, and ditch the humidifying setup.
Read up on a monotub. You could just place those trays into a monotub .
I would give your trays a light mist to get some beading of water droplets on the surface.
No need for a fan, its ok to have ambient air flow in the grow room, but not directly at the tubs.
Looks like its guna fruit for ya tho.
Alright, thanks! I misted them. I probably will go with a monotub next time. I figured since I’m at work all day this would be a good way to keep the humidity and airflow up.
I’m a kinda worried though. I’m starting to see a very faint bit of blue in both trays. Could the trays still get contaminated at this stage? I figured after the grain fully colonized I was basically in the clear if I kept the substrate in good conditions.
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morningWood
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Can I go 1% lme for agar plates and still get good growth?
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Smellyhobbit
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Re: Ask Quick Questions, Get Quick Answers [Re: morningWood]
#28631963 - 01/23/24 10:11 AM (4 days, 13 hours ago) |
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Why do that though?
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morningWood
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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit]
#28631982 - 01/23/24 10:25 AM (4 days, 12 hours ago) |
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I'm wanting to lower the nutrition, hopeing to see more rhizo growth. All my plates have gone tomentoes and I'm having a hard time telling if they are clean.
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Smellyhobbit
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Re: Ask Quick Questions, Get Quick Answers [Re: morningWood]
#28631995 - 01/23/24 10:33 AM (4 days, 12 hours ago) |
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Post pics of the plates. Tomentose is fine, you don’t need to chase rhizo growth. Learning to recognize healthy tomentose growth is more valuable than wasting time trying to force rhizomorphic growth.
1g is low as hell. Use 2% agar, 1.5% malt extract, and add 1g of nutritional yeast per 500ml of water if you want more rhizo growth.
Again, just learn to read tomentose growth. Tomentose is fine.
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Mr Piggy
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Registered: 09/29/11
Posts: 8,379
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Re: Ask Quick Questions, Get Quick Answers [Re: Luminous7] 1
#28631998 - 01/23/24 10:36 AM (4 days, 12 hours ago) |
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Quote:
Luminous7 said:
It looks ok , starting to show knots. But that set up is unnecessary dawg
That setup is one of the new vendors on here. I gotta say, shroomery is really just letting anyone be a vendor now. The shroomery seal of approval means nothing anymore.
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Mr Piggy
Big Dick Retard



Registered: 09/29/11
Posts: 8,379
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Quote:
psilocybenjamin said:
Alright, thanks! I misted them. I probably will go with a monotub next time. I figured since I’m at work all day this would be a good way to keep the humidity and airflow up.
When it comes to cubes, nothing in this hobby needs to be automated. A regular ol monotub will do a better job at keeping a stable and humid environment than that contraption will. To be honest, your sub looks very dry and that's probably where the bruising is coming from. Your sub is already shrinking well away from the trays and you haven't even had a flush yet.
I think you'll find monotubs to be a much cheaper and more effective method.
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morningWood
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Re: Ask Quick Questions, Get Quick Answers [Re: Mr Piggy]
#28632048 - 01/23/24 11:25 AM (4 days, 11 hours ago) |
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Quote:
Smellyhobbit said: Post pics of the plates. Tomentose is fine, you don’t need to chase rhizo growth. Learning to recognize healthy tomentose growth is more valuable than wasting time trying to force rhizomorphic growth.
1g is low as hell. Use 2% agar, 1.5% malt extract, and add 1g of nutritional yeast per 500ml of water if you want more rhizo growth.
Again, just learn to read tomentose growth. Tomentose is fine.
Definitely want to learn to read tomentoes!! I might just be paranoid.
Quote:
Smellyhobbit said: Post pics of the plates. Tomentose is fine, you don’t need to chase rhizo growth. Learning to recognize healthy tomentose growth is more valuable than wasting time trying to force rhizomorphic growth.
1g is low as hell. Use 2% agar, 1.5% malt extract, and add 1g of nutritional yeast per 500ml of water if you want more rhizo growth.
Again, just learn to read tomentose growth. Tomentose is fine.
I definitely want to recognize clean tomentoes. I might just be paranoid.


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Luminous7


Registered: 01/26/16
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Re: Ask Quick Questions, Get Quick Answers [Re: Mr Piggy] 1
#28632127 - 01/23/24 01:00 PM (4 days, 10 hours ago) |
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Quote:
Mr Piggy said:
Quote:
Luminous7 said:
It looks ok , starting to show knots. But that set up is unnecessary dawg
That setup is one of the new vendors on here. I gotta say, shroomery is really just letting anyone be a vendor now. The shroomery seal of approval means nothing anymore.
WHat? hahah
Quote:
Mr Piggy said:
Quote:
psilocybenjamin said:
Alright, thanks! I misted them. I probably will go with a monotub next time. I figured since I’m at work all day this would be a good way to keep the humidity and airflow up.
When it comes to cubes, nothing in this hobby needs to be automated. A regular ol monotub will do a better job at keeping a stable and humid environment than that contraption will. To be honest, your sub looks very dry and that's probably where the bruising is coming from. Your sub is already shrinking well away from the trays and you haven't even had a flush yet.
I think you'll find monotubs to be a much cheaper and more effective method.

The bruising is likey from dried out sub, or possibly from the humidifier hitting the surface, in any case its just blue bruising due to oxidation and nothing to be worried about.
But yeah, a monotub is designed to be "automated" you dont have to do shit with it. Theres a good write up explaining the thermo dynamics of how the monotub works to keep humidiy levels, introduce fresh air, and mitigate co2 levels. Check it out, its really good read.
https://www.shroomery.org/forums/showflat.php/Number/21371089/fpart/all
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Smellyhobbit
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Re: Ask Quick Questions, Get Quick Answers [Re: morningWood] 1
#28632130 - 01/23/24 01:04 PM (4 days, 10 hours ago) |
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Quote:
morningWood said:
Quote:
Smellyhobbit said: Post pics of the plates. Tomentose is fine, you don’t need to chase rhizo growth. Learning to recognize healthy tomentose growth is more valuable than wasting time trying to force rhizomorphic growth.
1g is low as hell. Use 2% agar, 1.5% malt extract, and add 1g of nutritional yeast per 500ml of water if you want more rhizo growth.
Again, just learn to read tomentose growth. Tomentose is fine.
Definitely want to learn to read tomentoes!! I might just be paranoid.
Quote:
Smellyhobbit said: Post pics of the plates. Tomentose is fine, you don’t need to chase rhizo growth. Learning to recognize healthy tomentose growth is more valuable than wasting time trying to force rhizomorphic growth.
1g is low as hell. Use 2% agar, 1.5% malt extract, and add 1g of nutritional yeast per 500ml of water if you want more rhizo growth.
Again, just learn to read tomentose growth. Tomentose is fine.
I definitely want to recognize clean tomentoes. I might just be paranoid.



Definitely cubensis mycelium. I’d probably take another transfer from each of those and send them after. They’re pretty good looking.
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Rohrschach
Stranger

Registered: 01/06/24
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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit]
#28632137 - 01/23/24 01:16 PM (4 days, 10 hours ago) |
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Trigger warning: I'm going to shoot some of my MS syringe into grains (wheat). Just because I ordered too many syringes, am waiting for my PF cakes to colonize and don't want to get into agar yet, since I have yet to find affordable containers/petri dishes.
I'm aware MS to grains isn't optimal. Still maybe someone cant tell me what the best technique for inoculation is with my setup? I'm pcing whole wheat right now. I prepared the lids with polyfill (bod's tek) and added some micropore just to make sure the polyfill doesnt move.
Now should I unscrew the jars in the SAB to introduce the syringe (risk of contam through big opening) or can I pierce the micropore/polyfill with the redhot needle and then add another piece of micropore (risk of contam because the new pieve of micropore isnt sterilized but maybe the polyfill will close it's hole just fine?)? Anyone have any have any inputs?
Edited by Rohrschach (01/23/24 01:19 PM)
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psilocybenjamin
Newb

Registered: 12/20/23
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Re: Ask Quick Questions, Get Quick Answers [Re: Luminous7]
#28632147 - 01/23/24 01:30 PM (4 days, 9 hours ago) |
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Thank you guys for the advice!
Well, damn. That sucks, but better news than contamination. Would misting it more help out the dry substrate?
I’m sure it was at field capacity when I mixed it with the spawn, but maybe the fan dried it out?
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TPython
A Fraction of the Whole


Registered: 12/01/12
Posts: 236
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Re: Ask Quick Questions, Get Quick Answers [Re: Rohrschach] 1
#28632148 - 01/23/24 01:31 PM (4 days, 9 hours ago) |
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Quote:
Rohrschach said: don't want to get into agar yet, since I have yet to find affordable containers/petri dishes.
Ive been using condiment cups for agar and its been working great. Hop on the agar train, you won't regret it!
The MS syringe itself is your biggest contam variable here, not the method of inoculation.
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morningWood
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Re: Ask Quick Questions, Get Quick Answers [Re: Rohrschach] 1
#28632153 - 01/23/24 01:34 PM (4 days, 9 hours ago) |
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Smellyhobbit- thanks for looking!! You can see in the last pic we're I took from, that's transfer is looking better. Again, thanks. I'll try 1.5% lme.
Edited by morningWood (01/23/24 01:37 PM)
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Rohrschach
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Re: Ask Quick Questions, Get Quick Answers [Re: TPython]
#28632158 - 01/23/24 01:44 PM (4 days, 9 hours ago) |
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Quote:
TPython said: Ive been using condiment cups for agar and its been working great. Hop on the agar train, you won't regret it!
Will do, but it's hard to get pc'able "condiment cups" or the like around here and petri dishes are very expensive. The same for whole grains, not even WBS is a thing here (contains mostly seeds and grains, but hulled).
Quote:
TPython said: The MS syringe itself is your biggest contam variable here, not the method of inoculation.
I was just thinking that unscrewing the lid and opening it a bit might be more dangerous than just piercing the polyfill (even though I'm aware that you'd have to unscre to do a2g or g2g anyways).
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Nerex
Stranger

Registered: 02/06/22
Posts: 176
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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
#28632162 - 01/23/24 01:47 PM (4 days, 9 hours ago) |
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I got a little half ass european PC which takes just 2 quarts jars barely... in order to make it run for like 3 hour I need to put in water enough to reach almost half the jars' height. Would be OK too for sterilizing?<
Maybe I put too much water in, but I don't want to risk to get a dry PC and leave that way.
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