|
rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
Posts: 6,767
Loc: In the Gills
Last seen: 6 hours, 9 minutes
|
|
Quote:
CowsPoopShrooms said:
Quote:
rumfor69 said:
Quote:
CowsPoopShrooms said: Quick Question: Is finding pins in an agar culture any indication of the cleanliness/contam-free of that specific cup/plate?
Not necessarily but a pin on a dish is a great way to get a clone. Transfer the pin to a new dish, then do a couple of series of transfers after that from the pin clone plate. If ur technique is good you should end up with a clean culture to then test on grain.
Ok, I've already transfered them to fresh agar. There was ~11 pins in 7-8 different cups I cut a couple larger ones in half and put them in their own cups totaling ~15 cups.
So, now I have a question about the clone: I read yesterday a post by RogerRabbit saying that just transferring a pin like that is just a T transfer and not a clone. I'll try to relocate it, but any thoughts on that? EDIT: Odd, everything I am finding now is saying the exact opposite. I'll keep looking.
Here ya go:
"Transferring pins from one petri dish to another is redundant. It also isn't cloning, but just transferring. Use that dish to inoculate grains if you want to test the strain. RR" https://www.shroomery.org/forums/showflat.php/Number/14792641#14792641
However, he has a whole bunch of posts talking just the opposite - specifically about using pins to clone.
I never thought I would do this but... I disagree with RR there. A clone is more genetically isolated. Instead of a multispore dish that has countless genetics in it a pin is going to have significantly less gene types even if it grew on that multispore plate. Plus it can be a good sign of a prolific fruiting variant that will fruit in less than ideal conditions. Add that to the pin growing on a less than clean plate and you could get something a little more contam resilient in addition to prolific traits.
|
CowsPoopShrooms
StillUphill


Registered: 06/08/22
Posts: 505
|
Re: Ask Quick Questions, Get Quick Answers [Re: PBJ710]
#27863004 - 07/14/22 08:38 PM (1 year, 6 months ago) |
|
|
Right, but it seems that his opinion
Edited by CowsPoopShrooms (10/14/22 02:32 PM)
|
Grogu


Registered: 07/02/22
Posts: 1,712
Last seen: 8 months, 11 days
|
Re: Ask Quick Questions, Get Quick Answers [Re: WyoMX]
#27863170 - 07/14/22 11:28 PM (1 year, 6 months ago) |
|
|
Quote:
WyoMX said:
Quote:
shroomsurf said: I guess they did send me Milo not Rye...lol
Had to take a second look...

Either one works just fine. Many people have grown many many pounds of mushrooms on all types of grain. My personal preference was to use milo as my master jar and g2g that to 10 jars of rye or wheat then spawn those to bulk. Rye being inferior or bad like that site is indicating is just wrong imo. Sounds like they just couldn't prep the rye correctly. Everyone has preferences so it's fine but claiming a grain sucks is weird. People say oats suck all the time but the people who use oats love em and defend them with their lives.
Thanks for your post!
|
Brian Jones
Club 27



Registered: 12/18/12
Posts: 12,340
Loc: attending Snake Church
Last seen: 1 day, 12 hours
|
|
Quote:
johntb said: Not millet but 50lb whole oats are $25 at my local tractor supply. If they’ll ever get it back in stock. Been there three time in last two weeks and still out. Maybe there on to me. Lol
My local Farm and Fleet store always has them, but the price recently doubled to $25.
-------------------- "The Rolling Stones will break up over Brian Jones' dead body" John Lennon I don't want no commies in my car. No Christians either. The worst thing about corruption is that it works so well,
|
DERRAYLD
Constructus


Registered: 05/13/02
Posts: 9,281
Loc: South Africa
Last seen: 14 hours, 34 minutes
|
Re: Ask Quick Questions, Get Quick Answers [Re: Grogu]
#27863323 - 07/15/22 06:11 AM (1 year, 6 months ago) |
|
|
Quote:
shroomsurf said: I guess they did send me Milo not Rye...lol
Had to take a second look...

Milo is good, enjoy.
|
rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
Posts: 6,767
Loc: In the Gills
Last seen: 6 hours, 9 minutes
|
Re: Ask Quick Questions, Get Quick Answers [Re: DERRAYLD]
#27863575 - 07/15/22 10:44 AM (1 year, 6 months ago) |
|
|
Question: With shiitake blocks, say using Aloha75, running in proper conditions, what is the average wet harvest yield per block?
Say for 100 blocks how much weight harvest?
Do you dunk blocks b4 fruiting or after first flush or not at all?
|
Grogu


Registered: 07/02/22
Posts: 1,712
Last seen: 8 months, 11 days
|
Re: Ask Quick Questions, Get Quick Answers [Re: DERRAYLD]
#27863599 - 07/15/22 11:04 AM (1 year, 6 months ago) |
|
|
Quote:
DERRAYLD said:
Quote:
shroomsurf said: I guess they did send me Milo not Rye...lol
Had to take a second look...

Milo is good, enjoy.
|
kirkeng
Stranger


Registered: 02/14/21
Posts: 805
Loc: Lost
|
Re: Ask Quick Questions, Get Quick Answers [Re: rumfor69]
#27863676 - 07/15/22 12:07 PM (1 year, 6 months ago) |
|
|
Rumfor, I can’t speak to that culture specifically, though I worked at farm a few back and we were getting 3-4lbs per 5lb block. Blocks consisted of wood chips, sawdust, mustard hulls, and spent brewers grain, forgive me I cannot remember the ratios from so long ago. The extent of our methods were hosing the blocks down after taking them from the bag. We never rehosed them again, just let em ride till they shriveled. Hope this helps.
|
rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
Posts: 6,767
Loc: In the Gills
Last seen: 6 hours, 9 minutes
|
Re: Ask Quick Questions, Get Quick Answers [Re: kirkeng]
#27863689 - 07/15/22 12:18 PM (1 year, 6 months ago) |
|
|
That's good info and coming from a farming experience vs hobby growing. I realize all sorts of variables can affect yield but I just want to have some kind of average idea for future plans. Seems like a reasonable idea would be 0.75lb yield per 1lb of block material.
|
kirkeng
Stranger


Registered: 02/14/21
Posts: 805
Loc: Lost
|
Re: Ask Quick Questions, Get Quick Answers [Re: rumfor69]
#27863751 - 07/15/22 01:17 PM (1 year, 6 months ago) |
|
|
I think that’s a good estimate! One other note I forgot, I can not confirm that this was useful though in our side by sides it appeared to initiate good first flushes. We would slap the blocks several times before unbagging them, in an effort to simulate ground vibrations from storms and such. I gotta get back to gourmets at some point…
|
AphexPin
Strange

Registered: 02/22/22
Posts: 464
|
Re: Ask Quick Questions, Get Quick Answers [Re: kirkeng]
#27863837 - 07/15/22 03:09 PM (1 year, 6 months ago) |
|
|
Advice for boil prep with millet? Was planning on bringing an adequate volume of water to boil, then turning off heat, adding millet and draining once I observe some bursts kernels.
|
natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
|
Re: Ask Quick Questions, Get Quick Answers [Re: AphexPin]
#27863871 - 07/15/22 03:41 PM (1 year, 6 months ago) |
|
|
@kirkeng- .75lb from 1lb of sub for any woodloving edible is a ridiculously high yield.
I mean shit a 5lb block at 60% water content is only 2lb of DRY sub material. If you're getting 4lb fresh mushrooms out of 2lb of dry sub material that's 200% biological efficiency which is pretty fucking difficult to achieve with any edible let alone shiitake which isnt a great yielder.
BE= fresh mushrooms/dry sub material x 100
4lb fresh mushrooms/2lb dry sub x 100 = 200% BE
My experience with all edibles is 1lb per 5lb block first flush is the benchmark and anything you get from flush 2 if you even do one is icing but not that thick.
--------------------
|
kirkeng
Stranger


Registered: 02/14/21
Posts: 805
Loc: Lost
|
Re: Ask Quick Questions, Get Quick Answers [Re: natedawgnow]
#27863907 - 07/15/22 04:18 PM (1 year, 6 months ago) |
|
|
Nate, that’s a fair point, I’m just basing off what I remember from back then so it’s possible my numbers aren’t 100% accurate. A few things I’ll say, we were far from accurate in our weighing our substrate out so there is discrepancy within that. We also ran blocks through several flushes, often blocks would be fruiting for several months. That being said I’m also taking estimates given what I remember from harvesting a room fulls total weight over my estimate for how many blocks, so bad estimations on either of those values could heavily skew. I should have stated better how I was making this estimate, also having it been a long time ago things can be skewed. I am far from an expert and base things off what I can remember from that time. I appreciate that breakdown though, it’s a good reminder to explain how I come to these things better, so thanks for that. I do recall us having very good yields on them though.
|
rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
Posts: 6,767
Loc: In the Gills
Last seen: 6 hours, 9 minutes
|
Re: Ask Quick Questions, Get Quick Answers [Re: kirkeng]
#27863943 - 07/15/22 05:15 PM (1 year, 6 months ago) |
|
|
The shiitake blocks in my sig produced just over 1lb each. They were some cheap tissue sample I got off a rando farm site. And I fruited them in a wrongly designed SGFC.
RR has pics of his Aloha75 blocks and you can't even see the block they're so covered in fruits.
0.75lb yield per 1lb of block(including the water cause they're 90% made of water) Doesn't seem unreasonable in proper conditions with a nice Aloha75 culture from Aloha Medicinals.
Just doesn't seem right to think that 200 blocks, 1,000lbs of material&water, Would only get you 200lbs of yield.
I don't think taking the water outta the BE math is correct.
Edited by rumfor69 (07/15/22 05:22 PM)
|
AphexPin
Strange

Registered: 02/22/22
Posts: 464
|
Re: Ask Quick Questions, Get Quick Answers [Re: rumfor69]
#27863979 - 07/15/22 06:07 PM (1 year, 6 months ago) |
|
|
Quote:
rumfor69 said: I don't think taking the water outta the BE math is correct.
That's how BE is defined.
|
rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
Posts: 6,767
Loc: In the Gills
Last seen: 6 hours, 9 minutes
|
Re: Ask Quick Questions, Get Quick Answers [Re: AphexPin]
#27863987 - 07/15/22 06:13 PM (1 year, 6 months ago) |
|
|
Ok, I'm learning stuff. I didn't know the water is excluded when it's so crucial to yield.
|
InfiniteLupercal
Newb

Registered: 07/14/22
Posts: 13
|
Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit]
#27863992 - 07/15/22 06:25 PM (1 year, 6 months ago) |
|
|
Quote:
Smellyhobbit said:
Quote:
Screwup said: Literally where are you getting 50LBs for 30$?
Quote:
InfiniteLupercal said:
Quote:
Smellyhobbit said: Hey off topic but Horus Aximand is a shithead.
If the Iron Warriors had stayed loyal y’all’s shenanigans would have been halted immediately.
TRUE! But Horus Lupercal is even worse! Haha! That is why I use the Luna Wolves symbol. I am a loyalist at heart. Loken was my favorite! Just glad someone recognized it that fast!  
Huuuuuge 40k fan here. Maybe some time I’ll post some of my painted minis. Im a commission painter as a side gig.
Wow! I would LOVE to see those! Let me know if you post them somewhere, and I will too. Though I am not that good. Poor eyesight and shaky hands don't make for the best mini-painter. Would LOVE to see your work!
-------------------- "You will try," he said. "But today you face the Emperor's sons and his warriors. We are the Luna Wolves, and this Legion is the anvil upon which you will be broken."
|
Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 11,144
Loc: Stables
Last seen: 11 hours, 59 minutes
|
Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome] 1
#27864002 - 07/15/22 06:35 PM (1 year, 6 months ago) |
|
|
Here’s a couple I posted to the random pics thread. One from this year one from last.
https://www.shroomery.org/forums/showflat.php/Number/27863999#27863999
|
offworld
Lurker


Registered: 08/07/21
Posts: 71
|
Re: Ask Quick Questions, Get Quick Answers [Re: AphexPin] 1
#27864025 - 07/15/22 06:56 PM (1 year, 6 months ago) |
|
|
Quote:
AphexPin said: Advice for boil prep with millet? Was planning on bringing an adequate volume of water to boil, then turning off heat, adding millet and draining once I observe some bursts kernels.
I like the ease of Cracks no prep millet, seems to be pretty popular here.
If you're wanting to boil them you might try FooMans WBS prep, probably works fine for millet only (the WBS I find is millet/milo/sunflower seeds). I've only just started using WBS though, maybe others can chime in with their favorite.
Edited by offworld (07/15/22 06:58 PM)
|
Rotnpins
🤮 Rotten-Pins 🍄



Registered: 01/11/22
Posts: 4,738
Loc: in (front of) the hood
Last seen: 1 year, 1 month
|
Re: Ask Quick Questions, Get Quick Answers [Re: offworld]
#27864033 - 07/15/22 07:07 PM (1 year, 6 months ago) |
|
|
Quote:
offworld said:
Quote:
AphexPin said: Advice for boil prep with millet? Was planning on bringing an adequate volume of water to boil, then turning off heat, adding millet and draining once I observe some bursts kernels.
I like the ease of Cracks no prep millet, seems to be pretty popular here.
easiest grain prep tek ever... jars don't come out very full tho, so sometimes I bump it up to 1.5 cups millet and 3/4 cup h2o per 1qt jar
|
|