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OfflineSmellyhobbit
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Re: Ask Quick Questions, Get Quick Answers [Re: UncleSillySiBen]
    #27861550 - 07/13/22 06:27 PM (1 year, 6 months ago)

Quote:

UncleSillySiBen said:
Quick Question: Can I use scotch tape to cover the holes on my lids after injecting?



What kind of jars are you doing?

PF jars don’t need to be covered, everything else should have protection you can inject through.


--------------------
A Love Letter to New Growers
A Guide for New Growers
Growth 2023 - A Year In Review

Grow more shrooms. Eat more ass. :mushroom:



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Offlinecozmyc
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Registered: 06/20/21
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Re: Ask Quick Questions, Get Quick Answers [Re: UncleSillySiBen]
    #27861667 - 07/13/22 07:58 PM (1 year, 6 months ago)

Ditto

If you have a layer of dry verm your good. If not some micro pore is good 👍 scotch tape would smother


--------------------
You're conscious population 2 stardust
----------------------
and that's valuable :sunny:


Edited by cozmyc (07/13/22 08:00 PM)


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OfflineGrogu
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Re: Ask Quick Questions, Get Quick Answers [Re: CowsPoopShrooms]
    #27861682 - 07/13/22 08:07 PM (1 year, 6 months ago)

Quote:

CowsPoopShrooms said:
Quote:

shroomsurf said:
Thanks guys for the "soaking answers" that really helps!

I have another question please? At this spawn magic site he says that rye truly sucks and there changing to Milo...lol

https://spawnmagic.com/product/3-lbs-grain-spawn/

I don't think they've changed the picture as I think Milo is small and round like a tiny BB.

Thanks




Quote from the page linked:

Quote:

NOTICE: Rye truly sucks. We’re switching back to Milo which is a large kernel Grain of sorghum...




Emphasis added

Which does not look like the pictures at duckduckgo: https://duckduckgo.com/?q=Milo+grain+&t=brave&iax=images&ia=images




When I ordered from them they sent me I think rye berry...

I got package really quick and with boomershroomer it's only $37 about for 9 lb including shipping...

:smile:


--------------------


Mushroom Cultivation Techniques...


Edited by Grogu (07/13/22 08:09 PM)


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OfflinePBJ710
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Re: Ask Quick Questions, Get Quick Answers [Re: Grogu]
    #27862029 - 07/14/22 04:39 AM (1 year, 6 months ago)

Quote:

shroomsurf said:
I got package really quick and with boomershroomer it's only $37 about for 9 lb including shipping...
:smile:




LOL...only $37 for 9lbs?!  Make your own and the cost will be 1/20th of that.


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InvisibleCowsPoopShrooms
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Registered: 06/08/22
Posts: 505
Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome] * 3
    #27862280 - 07/14/22 09:54 AM (1 year, 6 months ago)

Blue is PDA


Edited by CowsPoopShrooms (10/14/22 02:27 PM)


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OfflineHindsight
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Re: Ask Quick Questions, Get Quick Answers [Re: CowsPoopShrooms] * 1
    #27862292 - 07/14/22 10:02 AM (1 year, 6 months ago)

Nice work!


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OfflineRotnpins
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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #27862322 - 07/14/22 10:25 AM (1 year, 6 months ago)

I know that a lot of people don't use the outer ring of an agar plate because of possible contamination finding its way in from the edge of the plate... what if I was going to put a plate in cold storage and decided to roll the dice and cut out the outer ring first and toss it in a grain jar to see if it comes out clean.. if some contamination does succeed in getting in to the edge of the plate,  after the outer ring is already cut out, do you think it would likely stay in the outer part of the plate or does the new "outer ring" of agar have the same risk of contam as the original outer ring?

Hopefully that makes sense :shrug: I swear it made sense in my head


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InvisibleInfiniteLupercal
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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #27862323 - 07/14/22 10:26 AM (1 year, 6 months ago)

Hello!

So quick cultivation background; I have done quite a few BRF cakes with pretty good success. Ready to take my first dive into bulk growing. Already have a grain jar almost totally colonized. However, the spawn colonization has slowed or possible halted (inoculated about a month ago). My suspicion is too much liquid in the jar (I used liquid culture and was a little overzealous on the amount). My question is; with 75-80% of the grain covered in beautiful white mycelium, is there a way to utilize what HAS colonized, and safely get rid of the uncolonized grain? Or does it matter, and should I just put the loose uncolonized grain in with my bulk substrate and not worry about it? Sorry if this is overly vague or broad, or has been touched on anywhere. Just figured for a first post this wouldn't be too bad?!?! Thanks for any help!!!

(I can snap a picture and attach it if that would help?!)


--------------------
"You will try," he said. "But today you face the Emperor's sons and his warriors. We are the Luna Wolves, and this Legion is the anvil upon which you will be broken."


Edited by InfiniteLupercal (07/14/22 10:33 AM)


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InvisibleCowsPoopShrooms
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Re: Ask Quick Questions, Get Quick Answers [Re: CowsPoopShrooms] * 1
    #27862334 - 07/14/22 10:36 AM (1 year, 6 months ago)


:super:


Edited by CowsPoopShrooms (10/14/22 02:28 PM)


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OfflineHindsight
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Re: Ask Quick Questions, Get Quick Answers [Re: InfiniteLupercal]
    #27862359 - 07/14/22 10:58 AM (1 year, 6 months ago)

Quote:

InfiniteLupercal said:
Hello!

So quick cultivation background; I have done quite a few BRF cakes with pretty good success. Ready to take my first dive into bulk growing. Already have a grain jar almost totally colonized. However, the spawn colonization has slowed or possible halted (inoculated about a month ago). My suspicion is too much liquid in the jar (I used liquid culture and was a little overzealous on the amount). My question is; with 75-80% of the grain covered in beautiful white mycelium, is there a way to utilize what HAS colonized, and safely get rid of the uncolonized grain? Or does it matter, and should I just put the loose uncolonized grain in with my bulk substrate and not worry about it? Sorry if this is overly vague or broad, or has been touched on anywhere. Just figured for a first post this wouldn't be too bad?!?! Thanks for any help!!!

(I can snap a picture and attach it if that would help?!)




Pics might help give a better answer, depending on what the pics show. We might be able to identify some contamination in the jar depending on how it looks. If the jar is contaminated, most people don't bother trying to salvage portions of the jar because the success rate is rather low. You are best to just toss them and start over. If you REALLY want to salvage them and you don't have the culture backed up on an agar plate, I would suggest sterilizing a blade, letting it cool, then pulling out a couple of grains that look clean from the top and putting them on agar. Alternately you wrap a spoon in foil then bake it at 400F for 30 mins. Let it cool, then use it to scoop a tablespoon or two of grains from the top of the jar and dump those into a new clean jar of spawn and shake. That second option is likely to have a low success rate though. And either option should be done in your SAB or in front of your hood. Again, most of us just toss bad jars. If you have a yard, toss the grains under a bush and cover it with Blak Cow, compost, or coir and you are likely to get some good mushrooms outside:





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InvisibleInfiniteLupercal
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Registered: 07/14/22
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Re: Ask Quick Questions, Get Quick Answers [Re: Hindsight]
    #27862377 - 07/14/22 11:20 AM (1 year, 6 months ago)

Good to know! Thank you for the info! Here are the pictures. Second one is to show the excess liquid culture that pooled on the bottom. The brown on the mycelium is from that liquid. Otherwise, I don't think (I am probably wrong) there is any sign of contamination?





--------------------
"You will try," he said. "But today you face the Emperor's sons and his warriors. We are the Luna Wolves, and this Legion is the anvil upon which you will be broken."


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InvisibleCowsPoopShrooms
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Re: Ask Quick Questions, Get Quick Answers [Re: CowsPoopShrooms]
    #27862380 - 07/14/22 11:23 AM (1 year, 6 months ago)

wait for the others.


Edited by CowsPoopShrooms (10/14/22 02:29 PM)


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OfflineScrewup
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Re: Ask Quick Questions, Get Quick Answers [Re: InfiniteLupercal] * 2
    #27862382 - 07/14/22 11:25 AM (1 year, 6 months ago)

Yea that’s 100% contaminated.


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InvisibleInfiniteLupercal
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Registered: 07/14/22
Posts: 13
Re: Ask Quick Questions, Get Quick Answers [Re: InfiniteLupercal]
    #27862389 - 07/14/22 11:31 AM (1 year, 6 months ago)

Well dang! Thanks for the diagnosis! Any idea from what you can see how it became contaminated or how I could avoid that in the future?

Never used agar before, but heard a lot about it. Not sure how much it would cost to get everything I need to start working with agar, but already on kind of a tight budget. I will read up on it more here and see what I can come up with. Thanks again folks, I really appreciate it!


--------------------
"You will try," he said. "But today you face the Emperor's sons and his warriors. We are the Luna Wolves, and this Legion is the anvil upon which you will be broken."


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OfflineSmellyhobbit
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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #27862396 - 07/14/22 11:37 AM (1 year, 6 months ago)

You said you inoculated with liquid culture? Did you prepare the LC with a multi spire syringe? If so, that’s how contaminants got into your grain. The reason people use agar is to remove contaminants like competing spores and bacteria from your cubensis spores before inoculation. You can get lucky with spore to grain, but spore to liquid to grain has a lot more time for something bad to grow alongside your myc.


--------------------
A Love Letter to New Growers
A Guide for New Growers
Growth 2023 - A Year In Review

Grow more shrooms. Eat more ass. :mushroom:



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InvisibleInfiniteLupercal
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Registered: 07/14/22
Posts: 13
Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit]
    #27862403 - 07/14/22 11:46 AM (1 year, 6 months ago)

My apologies, and this will show how little I know. What is a multi spire syringe? Tried to ol' Google machine but it's getting confused on multi spore syringe.

Looks like it's time to go agar!


--------------------
"You will try," he said. "But today you face the Emperor's sons and his warriors. We are the Luna Wolves, and this Legion is the anvil upon which you will be broken."


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OfflineHindsight
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Re: Ask Quick Questions, Get Quick Answers [Re: InfiniteLupercal]
    #27862407 - 07/14/22 11:51 AM (1 year, 6 months ago)

That jar is extremely contaminated - bacterial. Just toss it outside and skip trying to save any grains from it.

How did you get the LC? And did you use a spore print or a spore syringe? You want to go spores to agar to grains. Can skip LC entirely unless you are growing large volumes.


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InvisibleInfiniteLupercal
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Registered: 07/14/22
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Re: Ask Quick Questions, Get Quick Answers [Re: Hindsight]
    #27862422 - 07/14/22 12:06 PM (1 year, 6 months ago)

LC I ordered from a favorite company of mine. And I went from syringes (again acquired from a favorite company of mine). Only ever taken one spore print, and not sure it worked or not.


--------------------
"You will try," he said. "But today you face the Emperor's sons and his warriors. We are the Luna Wolves, and this Legion is the anvil upon which you will be broken."


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OfflineHindsight
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Re: Ask Quick Questions, Get Quick Answers [Re: InfiniteLupercal] * 1
    #27862460 - 07/14/22 01:02 PM (1 year, 6 months ago)

Look up agar teks. I would suggest starting with no-pours. If you are getting LC or syringes, you will want to put them on agar to ensure they are clean. If you absolutely can't, or don't want to wait, make some BRF cakes and follow PF-Tek (using your LC syringe instead of a spore syringe). Agar is better but you can have a chance at success with BRF cakes.

https://www.shroomery.org/forums/showflat.php/Number/24144021


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OfflineSmellyhobbit
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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #27862465 - 07/14/22 01:06 PM (1 year, 6 months ago)

Hey off topic but Horus Aximand is a shithead.

If the Iron Warriors had stayed loyal y’all’s shenanigans would have been halted immediately.


--------------------
A Love Letter to New Growers
A Guide for New Growers
Growth 2023 - A Year In Review

Grow more shrooms. Eat more ass. :mushroom:



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