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Smellyhobbit
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Re: Ask Quick Questions, Get Quick Answers [Re: Rotnpins] 1
#27847644 - 07/03/22 11:52 AM (1 year, 6 months ago) |
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Excellent 👍
The oven method seems really simple.
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wild_aussie
Punk rocker til I die



Registered: 01/05/22
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Quote:
Oneleggedwarrior said: Quick question...
How long is agar (no pour method) ok to leave before inoculation and are they ok stored in cupboard?
I had mine in a cupboard for over 3 months and still good. In fact you know they are clean if last that long with nothing growing in them. I also use no pour
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Oneleggedwarrior
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Registered: 06/12/22
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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
#27847794 - 07/03/22 02:26 PM (1 year, 6 months ago) |
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Quick question?
Does it hit slightly harder with wet fruits? Had 1.2g mix of dry and wet munched and had a fantastic experience, more than expected with that dose! 🙏🏼
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Screwup
Googles your dumb questions


Registered: 01/27/22
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Yeah fresh fruits are supposed to be slightly or more potent than dry.
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Smellyhobbit
Actual Retard


Registered: 04/01/22
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Quote:
Oneleggedwarrior said: Quick question?
Does it hit slightly harder with wet fruits? Had 1.2g mix of dry and wet munched and had a fantastic experience, more than expected with that dose! 🙏🏼
Some people swear by fresh. Logically there has to be SOME potency loss while drying, right? Even if it’s really minimal.
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BagOfDicks
Procrastinator



Registered: 12/11/21
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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit] 1
#27847897 - 07/03/22 04:28 PM (1 year, 6 months ago) |
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I think it's very minimal if dried properly. Fresh panaeolus was my favorite yet!! But, I've also had some fresh mushrooms that were weak AF! Guess it's really more about potency. I will say that the visuals are probably slightly more intense fresh though--IME.
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Infinite Monkeys

Registered: 09/01/21
Posts: 276
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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
#27847929 - 07/03/22 05:11 PM (1 year, 6 months ago) |
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Quick question.
When it comes to making a flowhood, I've read that the depth of the filter should be 6" rather than 12".
The filters that are available where I live have a much smaller depth.
12" x 12" x 2.6" - PA @ 0.45m/s is 105 12" x 12" x 3.5" - PA @ 0.45m/s is 90 12" x 12" x 4.1" - PA @ 0.45m/s is 80
These are much smaller than 6".
Can I use these for a flow hood?
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Oneleggedwarrior
Stranger

Registered: 06/12/22
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Re: Ask Quick Questions, Get Quick Answers [Re: BagOfDicks]
#27848511 - 07/04/22 12:12 AM (1 year, 6 months ago) |
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Quote:
Screwup said: Yeah fresh fruits are supposed to be slightly or more potent than dry.
Quote:
Smellyhobbit said:
Quote:
Oneleggedwarrior said: Quick question?
Does it hit slightly harder with wet fruits? Had 1.2g mix of dry and wet munched and had a fantastic experience, more than expected with that dose! 🙏🏼
Some people swear by fresh. Logically there has to be SOME potency loss while drying, right? Even if it’s really minimal.
Quote:
BagOfDicks said: I think it's very minimal if dried properly. Fresh panaeolus was my favorite yet!! But, I've also had some fresh mushrooms that were weak AF! Guess it's really more about potency. I will say that the visuals are probably slightly more intense fresh though--IME.
Thanks for the answers, was wondering about potency of the fruit, guess will try all dry same dose next time and see how hits. Slightly preferred munching the dry fruit to be fair.
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Smellyhobbit
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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
#27852038 - 07/06/22 01:18 PM (1 year, 6 months ago) |
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Quick question:
Given the dirty nature of spores, how important is a sterile work environment for the creation of syringes? Does it make much of a difference if I do it out in the open vs a SAB?
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Rotnpins
🤮 Rotten-Pins 🍄



Registered: 01/11/22
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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit] 1
#27852042 - 07/06/22 01:20 PM (1 year, 6 months ago) |
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Quote:
Smellyhobbit said: Quick question:
Given the dirty nature of spores, how important is a sterile work environment for the creation of syringes? Does it make much of a difference if I do it out in the open vs a SAB?
Even with the inherently dirty nature of spore syringes, you still want to do everything you can to not increase the possibility/quantity of bacteria/mold spores... the cleaner it is, the more likely you are to have successful colonization
In my opinion, yes, it does make a difference. But that's just
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Smellyhobbit
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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome] 1
#27852045 - 07/06/22 01:22 PM (1 year, 6 months ago) |
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Yeah that seems common sense now that you’ve typed it out. Thanks.
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Cob
Ovoid fanboy


Registered: 07/01/22
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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit]
#27852164 - 07/06/22 02:39 PM (1 year, 6 months ago) |
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Noob here with a question about building a FH. I'm new to this, I have ordered and accumulated some things to work with. I understand some trying to cultivate the easiest and cheapest way possible. But in my opinion after doing some research it appears that I would be ahead to jump straight to the more established techniques. I have the ability to build a FH and want to go ahead and get one built. I'll be following c10's flow hood build to build mine: https://www.shroomery.org/forums/showflat.php/Number/24201748
My question is about the material and paint used. I have the plywood necessary to build one, I also have OSB. I'm guessing the OSB is not a great idea considering how rough the surface texture is. Plywood seems a better choice, possibly sanded on the interior surfaces that will be exposed to the work area. Then when it comes to paint, I've read many use VOC free paint. If I go with plywood, would it be pertinent to prime the plywood with KILZ?
Another question regarding materials, is there a substitute for plywood that would be better? I was thinking gauge metal/sheet metal or similar? The only reason I ask is because I have access to both carbon and stainless steel and the ability weld/fabricate the same box. It will be a little bit of money vs using plywood I already have. But I can easily fabricate a steel cabinet for under $150 or less. I have multiple blower motors though I need to calculate the cfm they produce.
I'm basically just trying to figure out how I'm going to build this FH. I'm building it around a 24"x24"x6" 99.99% @ 3mic filter backed by a second "large particulate" filter to help protect my HEPA filter.
I'm also going to make a simple SAB and begin with making Agar before I even attempt to inoculate any cakes/jars. My goal is to be able make clean Agar plates before I even unwrap my first MS syringe. I have a good PC and most of the materials. Once I have verified clean myc on Agar I'll be starting out with PF cakes in 1/2 pint jars. My goal is to successfully grow PE, I just want to do it right. It only recently occured to me that I could grow my own "fruits". After doing the research I'm stoked, I just want to take my time getting started and minimize contams.
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hambonemcgee
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Re: Ask Quick Questions, Get Quick Answers [Re: Cob]
#27852222 - 07/06/22 03:16 PM (1 year, 6 months ago) |
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Hi, noob here. I just inoculated my first set of jars yesterday and I thought I was good to go because I read all about the process and was pretty confident I hadn't forgotten anything. Well, the one thing I didn't do correctly was putting the needle of the syringe all the way through the vermiculite, I only put it about 1/2 inch into the jar instead of into the substrate. My question is, will just squirting the spores basically on top instead of penetrating the vermiculite ruin my jars? I was hoping it would just take longer, but I would appreciate any input you guys want to share.
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Sockadin



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Re: Ask Quick Questions, Get Quick Answers [Re: hambonemcgee]
#27852434 - 07/06/22 05:50 PM (1 year, 6 months ago) |
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Depends on if the innoculant gets down to the nutrious layer of the PF jar. If it is stuck in the Verm, it probably won't so anything.
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rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
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Re: Ask Quick Questions, Get Quick Answers [Re: Rotnpins] 2
#27852617 - 07/06/22 07:51 PM (1 year, 6 months ago) |
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Quote:
Rotnpins said:
Quote:
Smellyhobbit said: Quick question:
Given the dirty nature of spores, how important is a sterile work environment for the creation of syringes? Does it make much of a difference if I do it out in the open vs a SAB?
Even with the inherently dirty nature of spore syringes, you still want to do everything you can to not increase the possibility/quantity of bacteria/mold spores... the cleaner it is, the more likely you are to have successful colonization
In my opinion, yes, it does make a difference. But that's just 
I agree with this also. Reducing any additional contam vectors is always going to help. I make sterile spore collection jars for this reason. 1/4 pint wide mouth jar, metal wire bent like a little trivet stand, sfd lid setup, pc for 20mins. Set the cap on the wire stand in front of the FH, put the sfd lid back on, spores drop only touching sterile surfaces, cap touches sterile surfaces, when the print looks good, remove the cap and wire stand in front of the FH, put the lid back on, let it sit for days and dry out.
Then you only have to squirt sterile water in the jar, stir with the needle, and suck it up into the syringe. Or just swab with a sterile swab. All in front of the FH of course.
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Hindsight
Mad Scientist


Registered: 01/24/21
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Re: Ask Quick Questions, Get Quick Answers [Re: Cob]
#27852623 - 07/06/22 07:55 PM (1 year, 6 months ago) |
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Quote:
Cob said: Noob here with a question about building a FH. I'm new to this, I have ordered and accumulated some things to work with. I understand some trying to cultivate the easiest and cheapest way possible. But in my opinion after doing some research it appears that I would be ahead to jump straight to the more established techniques. I have the ability to build a FH and want to go ahead and get one built. I'll be following c10's flow hood build to build mine: https://www.shroomery.org/forums/showflat.php/Number/24201748
My question is about the material and paint used. I have the plywood necessary to build one, I also have OSB. I'm guessing the OSB is not a great idea considering how rough the surface texture is. Plywood seems a better choice, possibly sanded on the interior surfaces that will be exposed to the work area. Then when it comes to paint, I've read many use VOC free paint. If I go with plywood, would it be pertinent to prime the plywood with KILZ?
Another question regarding materials, is there a substitute for plywood that would be better? I was thinking gauge metal/sheet metal or similar? The only reason I ask is because I have access to both carbon and stainless steel and the ability weld/fabricate the same box. It will be a little bit of money vs using plywood I already have. But I can easily fabricate a steel cabinet for under $150 or less. I have multiple blower motors though I need to calculate the cfm they produce.
I'm basically just trying to figure out how I'm going to build this FH. I'm building it around a 24"x24"x6" 99.99% @ 3mic filter backed by a second "large particulate" filter to help protect my HEPA filter.
I'm also going to make a simple SAB and begin with making Agar before I even attempt to inoculate any cakes/jars. My goal is to be able make clean Agar plates before I even unwrap my first MS syringe. I have a good PC and most of the materials. Once I have verified clean myc on Agar I'll be starting out with PF cakes in 1/2 pint jars. My goal is to successfully grow PE, I just want to do it right. It only recently occured to me that I could grow my own "fruits". After doing the research I'm stoked, I just want to take my time getting started and minimize contams.
Many people have built with OSB and it worked ok. I built mine with nice sanded hardwood ply. I primed the inside with kilz. Stained the outside and then applied two coats of polyurethane. The idea is to prevent anything from growing on the wood (though even if it did, the filter should prevent it but why tempt fate).
Your first cabinet will be a learning experience - I am glad I used wood for my first build instead of metal (I can TIG weld). Metal might actually be cheaper but you end up re-doing some things as you dial everything in, such as your intake size, even the filter dims and fit can be tricky and it's nice to have the flexibility and workability of wood. Despite using a trusted vendor (not sponsored vendor of this site though), the motor they spec'd was WAY too big for the filter I bought from them. I ended up learning the flow hood math and finding the right blower myself, which meant more mods to the cabinet. Then the cabinet vibrated too much so I had to add in vibration isolating mounts and vibration isolating duct. Unless you think you are really, REALLY good and are only going to do everything once, I'd go with wood, even if more expensive.
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TwonkyD1
Stranger

Registered: 04/01/22
Posts: 4
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Re: Ask Quick Questions, Get Quick Answers [Re: Hindsight]
#27852733 - 07/06/22 08:57 PM (1 year, 6 months ago) |
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Quick question,
How come it seems like nobody is doing what RustyWhyte did for cubes, but for other species?
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hambonemcgee
Stranger

Registered: 07/06/22
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Re: Ask Quick Questions, Get Quick Answers [Re: rumfor69]
#27853737 - 07/07/22 03:45 PM (1 year, 6 months ago) |
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Thank you, I think that makes sense to me. I have one more syringe and six more jars, so I might go ahead and do those the right way and add them to my dozen jars. I have a feeling a few might not pop.
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WyoMX

Registered: 07/06/15
Posts: 2,100
Loc: PNW
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Re: Ask Quick Questions, Get Quick Answers [Re: TwonkyD1]
#27853759 - 07/07/22 04:07 PM (1 year, 6 months ago) |
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Quote:
TwonkyD1 said: Quick question,
How come it seems like nobody is doing what RustyWhyte did for cubes, but for other species?
Rusty whyte is a variety of cubes made by pastywhyte. Are you asking why other people aren't making crosses with different species or what's the actual question? Cubes are very forgiving and one of if not the easiest psychoactive mushrooms to grow but there are people out there crossing other species. It can take a while to stabilize and take to 7 or 8 generations though when they take 6+months from spore to harvest.
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Sockadin



Registered: 01/03/10
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Last seen: 2 months, 19 days
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Re: Ask Quick Questions, Get Quick Answers [Re: TwonkyD1] 1
#27853871 - 07/07/22 05:59 PM (1 year, 6 months ago) |
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Quote:
TwonkyD1 said: Quick question,
How come it seems like nobody is doing what RustyWhyte did for cubes, but for other species?
Cause it is hard to do and cubes are really easy to grow. Also other species may not have as many known phenotypes in the liniage to create things like pens envy pans. Or psilocybin cyan albino.
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