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OfflineSan Pedro GirlS
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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #28069882 - 11/26/22 09:12 AM (1 year, 2 months ago)

I’m close to making my first spore print with my first mushroom, but I’m confused on timing. This one has no veil and near flat cap, but it hasn’t dropped any spores that I can see. Do I take it now or wait?


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Re: As if: laminar flow hood [Re: Proposition 122]
    #28070681 - 11/26/22 07:47 PM (1 year, 2 months ago)

Quote:

Proposition 122 said:
I have a room air cleaner with a HEPA filter and an internal UV lamp. Any chance of utilizing this as a "ghetto" flow hood? Would this be better than a still air box?



It has its place. I run hepa filtration 24/7 in my dedicated mycology “lab” to keep the air generally cleaner. I kill all airflow in the lab, including the hepa filtration, 1hr before I use the still air box and have no problems. If I mess up and stir the air too much, at least it’s already cleaner air.🤷


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Re: Ask Quick Questions, Get Quick Answers [Re: Mushymang] * 2
    #28071379 - 11/27/22 10:48 AM (1 year, 1 month ago)

Quote:

Mushymang said:
Thanks for everyone’s help on this. I’m addition to the six plates I posted here I have 3 other sets (B+, Amazon PES, Colombian Rust Spore) that are also on t1. They look more like the weaker two of the six plates with less rope like myc. What do folks do when that occurs? Transfer the best sections to a new plate or start over with a germination plate from MSS? Attaching a pic of the weaker plates again for reference.




I’m in no rush, so what I do is let plates grow out until they pin. Then, once that mycelium outcompetes it’s brothers and proves it can fruit, I transfer that pin to a new plate and send that to grain when it grows out.


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Re: Ask Quick Questions, Get Quick Answers [Re: Polk_Audio3]
    #28072524 - 11/28/22 05:40 AM (1 year, 1 month ago)

Quote:

Polk_Audio3 said:


She opened I get off wrk at 430EST I’ll have a new FC maybe Shrooms be ok until then they opened over night I can tell the plate wit a little water helped that one



Harvest that center cake before you go to work and get it drying or printing!


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Re: Drying temps [Re: Proposition 122]
    #28072536 - 11/28/22 05:56 AM (1 year, 1 month ago)

Quote:

Proposition 122 said:
My Nesco is only 425 watts but heats to (non-adjustable) 180-degrees. Isn't this too hot? I haven't used it on mushrooms yet (only apples) but 180 seems awfully hot. What is the upper limit without damaging the little critters? :confused:



That temp will degrade the alkaloids a bit, but so will 160°F. It’s more important to dry them fast than worry about temp.

The mushrooms are acidic to begin with and once harvested, they absorb co2 from the air which causes rapid degradation via carbonic acid hydrolysis, et al, so get the water out fast. Alternatively, psilocybin/psilocin are stable under basic conditions, so making a tea, etc with potable tap water, which is buffered, will stop most degradation other than oxidation.


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OfflineSan Pedro GirlS
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Re: Drying temps [Re: Rusty2096]
    #28072638 - 11/28/22 08:37 AM (1 year, 1 month ago)

Quote:

Rusty2096 said:
Unless you are talking about alkaloids other than psilocyn/cybin, that is just not true.



For clarity, I was referring to psilocybin/psilocin specifically.


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OfflineSan Pedro GirlS
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Re: Ask Quick Questions, Get Quick Answers [Re: Guerrilla] * 1
    #28072672 - 11/28/22 09:03 AM (1 year, 1 month ago)

Quote:

W1llyWonka said:
Ello! Getting a shitload of sidepins, should I move the cake to bigger tote or just let it ride and get squished fruits? Guess Ill go back to using liner in future as this aint my jam.




I had tons of side pins on my shoeboxes and just let them grow squished. It was a pain and I regret not pulling it out to set in a monotub.  My vote is definitely to take it out.


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OfflineSan Pedro GirlS
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Re: Drying temps [Re: Rusty2096]
    #28072792 - 11/28/22 10:51 AM (1 year, 1 month ago)

Quote:

Rusty2096 said:
Quote:

San Pedro Girl said:
Quote:

Rusty2096 said:
Unless you are talking about alkaloids other than psilocyn/cybin, that is just not true.



For clarity, I was referring to psilocybin/psilocin specifically.




I mean those start degrading at much much higher temperatures. 150c/257f still have 0% degradation effect so...they are ultra safe in any dehydrator - please don't tell people it will degrade from heat when it won't. That's just common misbelief.



To the best of my knowledge and applicable peer reviewed studies, I gave correct information in that post. Of course I, along with any of those studies could be wrong, so can you elaborate? I’m all for learning here.🙂

Check these out if you’re interested.

Gotvaldová K, Hájková K, Borovicˇka J, Jurok R, Cihlářová P, Kuchař M. Stability of psilocybin and its four analogs in the biomass of the psychotropic mushroom Psilocybe cubensis. Drug Test Anal. 2021;13:439–446.

John T. Cody,
Chapter 4 Hallucinogens,
Editor(s): M.J. Bogusz,
Handbook of Analytical Separations,
Elsevier Science B.V.,
Volume 6,
2008,
Pages 175-201,
ISSN 1567-7192,
ISBN 9780444522146


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OfflineSan Pedro GirlS
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Re: Drying temps [Re: SingularFusion]
    #28072838 - 11/28/22 11:30 AM (1 year, 1 month ago)

Quote:

Nef said:
Quote:

San Pedro Girl said:
applicable peer reviewed studies🙂




got a link to that please Pedro Girl?

would be keen to see it

:cheers:



I added a couple to
Quote:

Nef said:
Quote:

San Pedro Girl said:
applicable peer reviewed studies🙂




got a link to that please Pedro Girl?

would be keen to see it

:cheers:



Sure, glad to help. I just have these citations handy, but a google search should pull them. I edited to add these to a previous post as well.

Gotvaldová K, Hájková K, Borovicˇka J, Jurok R, Cihlářová P, Kuchař M. Stability of psilocybin and its four analogs in the biomass of the psychotropic mushroom Psilocybe cubensis. Drug Test Anal. 2021;13:439–446.

John T. Cody,
Chapter 4 Hallucinogens,
Editor(s): M.J. Bogusz,
Handbook of Analytical Separations,
Elsevier Science B.V.,
Volume 6,
2008,
Pages 175-201,
ISSN 1567-7192,
ISBN 9780444522146


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OfflineSan Pedro GirlS
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Re: Drying temps [Re: SingularFusion]
    #28072925 - 11/28/22 12:25 PM (1 year, 1 month ago)

Quote:

Nef said:
Thanks but I am not sure if they support your point

The degredation of alkaloids they discuss starting from 100°C is discussing alkaloids in solution of given solvent

I mean they talk about drying fruit bodies in the dark at room temperature as being the optimal for alkaloid preservation? We know from experience that is simply not the case. They did not dry any fruit bodies at dehydrator temps in the study

So while I appreciate you providing the link I don't understand how that supports your assertion that heat ruins potency when drying



I think the opposite is true. Heat causes drying to occur faster, thus preserving most of the potency, not ruining it.


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OfflineSan Pedro GirlS
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Re: Drying temps [Re: DharmaForKarma]
    #28072927 - 11/28/22 12:26 PM (1 year, 1 month ago)

Quote:

DharmaForKarma said:
Quote:

Smellyhobbit said:
Freezing is much more damaging. Exposure to moisture and light is the biggest concern. The faster you dry them, the more you save.




Bingo.

The rules are simple:  Upon harvest dry immediately, hot, for long enough, and keep away from light and air and moisture. No food dehydrator has a setting that will degrade mushrooms.

There are lots of “feelings” about harming the precious fruits.

Don’t. Just don’t. We baby them from spore to pin but once they’re cut they are ready to face the world. After we take the water out.

Dry quickly, hot, long, then protect from the elements.



Exactly!


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Re: Drying temps [Re: Excess Taters]
    #28072933 - 11/28/22 12:29 PM (1 year, 1 month ago)

Quote:

Excess Taters said:
Here's a direct link.

Study link pdf


https://postimg.cc/XptbZtLH

Either way, it showed a very low level of degradation at 75°c, or ~165 ish.  Like... 5-10% compared to lower numbers, I think that's more confirmation that we're correct in basically ignoring that minimal degradation. 

I don't know why the image doesn't want to link directly, but the table from the study with temps is linked below the study now.



Yep, that’s exactly what I said in my original post. For some reason a lot of people keyed in on me saying dehydrating causes a bit of degradation, which is true, but it’s still the best practical way to preserve.


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Re: Drying temps [Re: SingularFusion]
    #28072938 - 11/28/22 12:33 PM (1 year, 1 month ago)

Quote:

Nef said:
Thanks but I am not sure if they support your point

The degredation of alkaloids they discuss starting from 100°C is discussing alkaloids in solution of given solvent

I mean they talk about drying fruit bodies in the dark at room temperature as being the optimal for alkaloid preservation? We know from experience that is simply not the case. They did not dry any fruit bodies at dehydrator temps in the study

So while I appreciate you providing the link I don't understand how that supports your assertion that heat ruins potency when drying



3.2 and figure 1 of the study shows a quick summary.


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OfflineSan Pedro GirlS
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Re: Drying temps [Re: Rusty2096]
    #28072942 - 11/28/22 12:36 PM (1 year, 1 month ago)

Quote:

Rusty2096 said:
Welp, if you truly are all about learning, then the few posts above this one should do just that :super:



Most people just said I was wrong and then parroted my original post.🤷 I may be missing something.


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Re: Drying temps [Re: Smellyhobbit]
    #28072951 - 11/28/22 12:40 PM (1 year, 1 month ago)

Quote:

Smellyhobbit said:
That’s the part we’re honing in on, yes. But it isn’t the dehydration temps that cause it. It’s the fact that they’re sitting picked in a dehydrator and they’re not dry yet. Temps aren’t the issue.



Gotcha! So the general consensus is that wet at any temp is equally bad? I was under the impression that the hydrolysis was accelerated by heat, but that the minimal degradation due to the extra heat was less than the increased time wet at lower temps.


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Re: question [Re: Rusty2096] * 1
    #28073599 - 11/28/22 07:29 PM (1 year, 1 month ago)

Quote:

Rusty2096 said:
Stop fucking around with the title folks

Edit: QQ - Do you guys think when shaking jars pretty hard, the grains coming in contact with the SFD from the inside could cause a jar to become bacterial?





If a jar becomes bacterial from shaking grains against the SFD, then it was a bad SFD. A .3 mic SFD has pores that are one quarter the size of even the smallest known bacteria.


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Re: question [Re: Rusty2096]
    #28073712 - 11/28/22 08:46 PM (1 year, 1 month ago)

Quote:

Rusty2096 said:
@SPD: ty - Sorry please don't think I'm trying to nail you but some endospores are as small as 0.5m when mature. Those are the typical ones but I assume there are also smaller ones. That said they prob aren't my problem :shrug:



Hey no worries, I didn’t know that so thanks for sharing. I just had to google to figure out what an Endospore is!🤣


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Re: question [Re: Smellyhobbit]
    #28074519 - 11/29/22 01:15 PM (1 year, 1 month ago)

Quote:

Smellyhobbit said:
Awesome, thanks 👍

Got my discs and lids. Going to prep them up to the RTV. Walmart doesn’t have anything, unsurprisingly. I’ll just Amazon it. Won’t be able to prep for a few days anyway.



Autopart store near you will have it.  Autozone or oreileys?


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Re: question [Re: Wishery] * 1
    #28074585 - 11/29/22 01:57 PM (1 year, 1 month ago)

Quote:

Wishery said:
how do ya'll clean your quart jars if your hand can't fit inside



Bottle brush on a drill.


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Re: Ask Quick Questions, Get Quick Answers [Re: cooleko]
    #28075836 - 11/30/22 10:29 AM (1 year, 1 month ago)

Quote:

cooleko said:
Attempting to breed a mycelium that can kill everything it competes with might just make it unsuitable for consumption or kick off an arms race to create a superbacteria that eats your face with glee because it gave up on competing with the mycelium.



Reminds me of the guy who got hospitalized in the intensive care unit and almost died after he injected himself with shroom tea and the mycelium started to colonize him.


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