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tholos
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Re: Ask Quick Questions, Get Quick Answers [Re: DreadedFarms]
#28218277 - 03/06/23 11:43 PM (10 months, 17 days ago) |
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what are your indicators that you're seeing that make you think there is contam?Quote:
DreadedFarms said:

This looks bacterial to me. Could someone confirm? It's APE revert, spores to LC, to this grain jar. Yes, spores to lc, hence my assumption it's bacterial.
What are you seeing that is making you question? Got any other pics from different angles? etc.
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tholos
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Re: Ask Quick Questions, Get Quick Answers [Re: DreadedFarms]
#28218850 - 03/07/23 12:12 PM (10 months, 16 days ago) |
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Quote:
DreadedFarms said: Variations in mycelium color, a few bare grains, looks like it's trying to pin early...
It does look a bit yellow, myc piss caused by contam potentially?
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tholos
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Re: Ask Quick Questions, Get Quick Answers [Re: Excess Taters]
#28227066 - 03/12/23 11:17 PM (10 months, 11 days ago) |
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Anyone familiar with these chunky growths happening on the top of my grain?
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tholos
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Re: Ask Quick Questions, Get Quick Answers [Re: Grogu]
#28227120 - 03/12/23 11:56 PM (10 months, 11 days ago) |
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Quote:
Grogu said:
Quote:
tholos said:



Anyone familiar with these chunky growths happening on the top of my grain?
What strain is that?

Tidal Wave 2/Enigma isolation
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tholos
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Re: Ask Quick Questions, Get Quick Answers [Re: dowodenum]
#28227139 - 03/13/23 12:12 AM (10 months, 11 days ago) |
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Quote:
dowodenum said: Could just be how that strain likes to knot up. My PM tubs and PE6 jars definitely get like that sometimes.
Does this mean they're trying to fruit? Possibly in response to adverse conditions (contam, not enough GE, too high moisture content, etc.)
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tholos
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Jars inoculated 02.27
Break and shake 03.13
Does this look contam'd? (looking specifically at grains pressed against glass and large amount of moisture present)
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tholos
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Re: Ask Quick Questions, Get Quick Answers [Re: Way]
#28234547 - 03/18/23 01:46 AM (10 months, 6 days ago) |
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Quote:
Way said:
Quote:
tholos said:


Jars inoculated 02.27
Break and shake 03.13
Does this look contam'd? (looking specifically at grains pressed against glass and large amount of moisture present)
Looks like it to me. Also the consistency of the mycelium. It's still "reaching" even though it's fully colonized.

I think it is. I think the myc is trying to get out of the jar. It looks like its knotting up on the top of layer of grains. Should I toss? Isolation is Enigma and it has a long fruiting time, so sending it to grain already contam'd would be setting myself up for failure IMO
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Re: Ask Quick Questions, Get Quick Answers [Re: Way]
#28234564 - 03/18/23 02:07 AM (10 months, 6 days ago) |
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Quote:
Way said:
Quote:
tholos said:
Quote:
Way said:
Quote:
tholos said:


Jars inoculated 02.27
Break and shake 03.13
Does this look contam'd? (looking specifically at grains pressed against glass and large amount of moisture present)
Looks like it to me. Also the consistency of the mycelium. It's still "reaching" even though it's fully colonized.

I think it is. I think the myc is trying to get out of the jar. It looks like its knotting up on the top of layer of grains. Should I toss? Isolation is Enigma and it has a long fruiting time, so sending it to grain already contam'd would be setting myself up for failure IMO
It just checks too many boxes. the rhyzomorphic tendrils, the uncolonized wet grain,the color. Only you can decide if it is worth it to toss, but I'd say it's definitely not healthy.
I've got clean plates and an LC that I streaked onto a plate earlier this week that looks good. At this point all I'll have lost is time. I'm assuming the contam came from my grain prep and/or storage/handling of jars during colonization. All of my agar work seems to look good
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Re: Ask Quick Questions, Get Quick Answers [Re: ShroomNugget]
#28236493 - 03/19/23 12:29 PM (10 months, 4 days ago) |
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Opinions on if this is contaminated?
Main concern is condensation build up on the inside of the jar, and weak/impartial colonization of some grains
Edited by tholos (03/19/23 12:30 PM)
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Re: Ask Quick Questions, Get Quick Answers [Re: Mycovester]
#28248943 - 03/27/23 08:51 AM (9 months, 28 days ago) |
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Quote:
Mycovester said:

How are these two looking? PE on rye. Colonised quickly now they are consolidating.
I can't make my mind up if there's bacterial war going on or not. There's no yellow juice and the mycelium was all light and fluffy with until I let them consolidate.
Quote:
Screwup said: I’d send em.
Thanks. I am going to send them this fine day.
They look pretty good to me, they aren't what I would call "obviously" bacterial
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Re: Ask Quick Questions, Get Quick Answers [Re: CocaineBuffet] 2
#28250068 - 03/27/23 09:30 PM (9 months, 27 days ago) |
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Edited by tholos (03/27/23 09:31 PM)
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tholos
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Re: Ask Quick Questions, Get Quick Answers [Re: ReverendMyc]
#28256239 - 03/31/23 01:35 PM (9 months, 23 days ago) |
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Anyone have experience with so called "no prep" grain methods?
I'm thinking specifically of a tek where you weigh out a ratio of grain to water in a quart jar then load into the PC immediately. If I'm using SFDs how do I avoid them coming out of the PC soaking wet? I'm assuming I can not avoid that and the best thing to do would be to let my jars dry in a SAB so the wet filter does not become a point of contamination
Anyone else have experience or insight into this situation?
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tholos
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Re: Ask Quick Questions, Get Quick Answers [Re: Screwup]
#28259244 - 04/02/23 02:15 PM (9 months, 21 days ago) |
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Quote:
Screwup said:
I’d say yes. How long has it been like that? I’d still send it tbh.
Wait is that blue in the jar?

A tiny bit, I'd send as well
Edited by tholos (04/02/23 02:16 PM)
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Re: Ask Quick Questions, Get Quick Answers [Re: NFLProof] 3
#28259575 - 04/02/23 06:07 PM (9 months, 21 days ago) |
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Quote:
NFLProof said: These aren't PC'able are they? I can't seem to find what they're made of. In order to be PC'able they have to be polyethylene correct?
60 Pack Sterile Plastic Petri Dishes with Lid, 90mm Dia x 15mm Deep (90MM-60PACK) https://a.co/d/iKHvV7S
PP5 is what you're looking for, PP5 is polypropylene
Anything being sold as "sterile" has been pre-sterilized and often times are a one use thing
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Re: Ask Quick Questions, Get Quick Answers [Re: ShroomNugget] 1
#28261196 - 04/03/23 08:31 PM (9 months, 20 days ago) |
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Quote:
ShroomNugget said:
Quote:
ShroomNugget said: Why are these guys so bruised up in this little patch? I assume it's a sign they are in need of something. More FAE? More water?
The bigger guy I circled on the other cake hasn't changed size in two days. Is it possible its done and I should harvest it? This is APE and they're still quite small. Standard sharpie in there for scale. Input would be much appreciated! My first grow, with APE none the less, but they seem to be turning out quite well.

Anybody out there with APE experience that can help a brutha out?
It is normal for APE caps to turn blue look up "Starry Night" APES or SNAPES to see an extremely blue cap phenotype -- nothing wrong with it!
Edited by tholos (04/03/23 08:32 PM)
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tholos
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Quote:
trollettin_reyiz said: Thanks, Derralyd.
Fine, back to square 1 then.
I have two big plates. The more-grown one is the first clone. Then I made the second one from the first. I'll inoculate more agar plates today. Which one do you think I should use? And if possible, which parts would be your favorite to clone?

First one looks sketchy, second one does not look promising either IMO
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Re: Ask Quick Questions, Get Quick Answers [Re: Yahra] 1
#28266156 - 04/06/23 10:31 PM (9 months, 17 days ago) |
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Quote:
Yahra said: While making my syringe for Pf-tek i did something stupid.
Instead of removing the needle cap in my SAB to suck up the spores i removed the entire needle.

How bad is it? I know that syringes are "inherently dirty" , but i think i now have a super dirty one.
Should i start over?
If you were working in your SAB and you didn't have any airborne contaminants due to your movement you should be golden
Also PF Tek is a tek that is specifically resistant to contamination from spore syringes so I'm sure if contamination was present in your syringe that would be a bigger issue than you momentarily leaving the end of it exposed to open air for a few seconds
Edited by tholos (04/06/23 10:32 PM)
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Re: Ask Quick Questions, Get Quick Answers [Re: RoscoeReturns]
#28267442 - 04/07/23 06:46 PM (9 months, 16 days ago) |
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Quote:
RoscoeReturns said:
Quote:
stroopwafelman said: Is there any specific reason why grain jar lids are screwed on loosely before PCing despite the lid having a filter on it?
I do it because the plastic lids I use will warp if screwed on tight during the PC cycle.
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Re: Ask Quick Questions, Get Quick Answers [Re: San Pedro Girl]
#28268363 - 04/08/23 08:46 AM (9 months, 16 days ago) |
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What brand/vendor are people using for single use pre sterilized petri dishes?
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tholos
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Re: Ask Quick Questions, Get Quick Answers [Re: ShroomNugget]
#28269424 - 04/08/23 11:29 PM (9 months, 15 days ago) |
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Quote:
ShroomNugget said: Nah they are inoculated culture plates coming in. I plan to inoculate some jars of LC I already have ready and using those to grow some stuff out. I'd like to keep the original cultures long term.
What are some good slants/tubes and what kind do I want? Borosilicate glass with plastic screw on caps?
Edit: would these be good? https://a.co/d/f4N1urD
I would go with plastic centrifuge tubes
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