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OfflineShroomNugget
Mycologist Wannabe
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Registered: 02/10/23
Posts: 355
Last seen: 25 days, 21 hours
Re: Ask Quick Questions, Get Quick Answers [Re: Mycolorado]
    #28189519 - 02/15/23 10:43 PM (11 months, 5 days ago)

Hey fellas, I just inoculated for the first time using 1/2 pint BRF cakes via PF TEK. Im looking to clean up an area for the SGFC to settle in. Unfortunately my entire house is carpeted except my overly stuffed storage room.



That being said this isn't ideal but the best spot with the least amount of traffic, in my basement below the stairs. It stays about 65F this time of year. 😅 I plan to place the SGFC on that table and elevated on a wire shelf, up about 8".

I need to do a deep clean after I make some room for all that junk in the way. What's my best odds of success for sterilizing the area? I'm going to dust and vacuum really well, clean the walls and spray everything down with ISO.

Should I buy and use some carpet or fabric disinfectant spray? Anybody have any experience with this? Any particular brand/type or is it useless?


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Edited by ShroomNugget (02/15/23 10:43 PM)


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OfflineShroomNugget
Mycologist Wannabe
I'm a teapot User Gallery


Registered: 02/10/23
Posts: 355
Last seen: 25 days, 21 hours
Re: Ask Quick Questions, Get Quick Answers [Re: micoshroom]
    #28193113 - 02/18/23 01:57 PM (11 months, 2 days ago)

Hey fellas, I'm back! First timer here and I inoculated my jars 70 hours ago. The wait is killing me but I noticed this in one jar beginning about 24 hours after inoculation. Am I a Myc daddy or is this contam?!




This is how the rest of that jar, and all other jars look.



Thanks in advance! 😀


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OfflineShroomNugget
Mycologist Wannabe
I'm a teapot User Gallery


Registered: 02/10/23
Posts: 355
Last seen: 25 days, 21 hours
Re: Ask Quick Questions, Get Quick Answers [Re: hazyhorse]
    #28193123 - 02/18/23 02:08 PM (11 months, 2 days ago)

Spore syringe, keeping it simple on my first go. I looked at the picture from yesterday and it's exactly the same hasn't spread in 24 hrs.


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OfflineShroomNugget
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I'm a teapot User Gallery


Registered: 02/10/23
Posts: 355
Last seen: 25 days, 21 hours
Re: Ask Quick Questions, Get Quick Answers [Re: LadysKnight]
    #28193237 - 02/18/23 03:02 PM (11 months, 2 days ago)

Quote:

LadysKnight said:
Do new people read anything besides the replies to their own posts?

Usually the answers are all around them in other active threads/posts.

Specifically spores to grain and tub automation.

Every day.

All day.




Why are you complaining abut this in about a Q and A thread? I don't get it, lol. Nobody is forcing you to answer questions.

The answer is: There are thousands of threads and a lot of information on every subject. So much so that some information is contradicting other information. Or some posts are so poorly worded, or just plain OLD that it's hard to trust the info. Friendly people answering questions makes it 5000x easier on us noobs.

Example: I did a week's worth of research before buying stuff to start out. Weekend one I spent like 3 hours drilling holes for a SGFC. So much talk about SGFC is the best way to start! Two days ago I found out a water box is way better for a climate like mine, especially in the winter. This is probably a dozen pages back now, but someone mentioned it when I asked a different question. I can't say enough how happy I am that he mentioned the tek to me because IMO it sounds way easier than a SGFC. So it's extremely helpful and we are grateful.

Edit: I STILL can't find the best, cheapest solution for a 6500k full spectrum light because every thread I find on light is a fight regarding if it's necessary or not. I don't care I just want a cheap ass light that's bright enough to work, because why not? Can't hurt.


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Edited by ShroomNugget (02/18/23 03:31 PM)


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OfflineShroomNugget
Mycologist Wannabe
I'm a teapot User Gallery


Registered: 02/10/23
Posts: 355
Last seen: 25 days, 21 hours
Re: Ask Quick Questions, Get Quick Answers [Re: hazyhorse]
    #28193289 - 02/18/23 03:32 PM (11 months, 2 days ago)

I just grabbed a couple bulbs and a hanging light socket with switch on Amazon. There are all sorts of $20ish LEDs for growing but I feel they didn't have enough light/power. I just wanted a simple 10w LED bulb as I read that's about perfect (in a single thread, only info I could find).


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Edited by ShroomNugget (02/18/23 03:34 PM)


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OfflineShroomNugget
Mycologist Wannabe
I'm a teapot User Gallery


Registered: 02/10/23
Posts: 355
Last seen: 25 days, 21 hours
Re: Ask Quick Questions, Get Quick Answers [Re: LadysKnight]
    #28193888 - 02/18/23 10:43 PM (11 months, 2 days ago)

Quote:

LadysKnight said:
Quote:

Weioo said:
Quote:

LadysKnight said:
Do new people read anything besides the replies to their own posts?

Usually the answers are all around them in other active threads/posts.

Specifically spores to grain and tub automation.

Every day.

All day.




Why are you complaining abut this in about a Q and A thread? I don't get it, lol. Nobody is forcing you to answer questions.

The answer is: There are thousands of threads and a lot of information on every subject. So much so that some information is contradicting other information. Or some posts are so poorly worded, or just plain OLD that it's hard to trust the info. Friendly people answering questions makes it 5000x easier on us noobs.

Example: I did a week's worth of research before buying stuff to start out. Weekend one I spent like 3 hours drilling holes for a SGFC. So much talk about SGFC is the best way to start! Two days ago I found out a water box is way better for a climate like mine, especially in the winter. This is probably a dozen pages back now, but someone mentioned it when I asked a different question. I can't say enough how happy I am that he mentioned the tek to me because IMO it sounds way easier than a SGFC. So it's extremely helpful and we are grateful.

Edit: I STILL can't find the best, cheapest solution for a 6500k full spectrum light because every thread I find on light is a fight regarding if it's necessary or not. I don't care I just want a cheap ass light that's bright enough to work, because why not? Can't hurt.




Didn't mean to trigger anyone. But this is a QnA thread, as you mentioned. And I asked a question.

Whether or not searching is hard and old threads exist, there are current threads and posts within the same thread (this one for example) that would answer most questions if anyone bothered to read. And again, not referring to anyone specifically, just the general new peep mentality.

Thank you for the reply.




Nah not triggered bruh. I also want to add that the search probably isn't what it was. Literally half the search results are threads saying "USE THE SEARCH" roofles.


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OfflineShroomNugget
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I'm a teapot User Gallery


Registered: 02/10/23
Posts: 355
Last seen: 25 days, 21 hours
Re: Ask Quick Questions, Get Quick Answers [Re: Excess Taters]
    #28203946 - 02/25/23 11:38 AM (10 months, 26 days ago)

Friggin electrical company just ruined my power while fixing others. Place is down to low 50s, high 40s and possibly going to drop below 40.

My 1/2 pint BRF jars started condensating on the inside of the jar walls. Is there a certain temperature that will kill/highly effective my mycelium? I'm staying else where and wondering if I should just box them up and bring them with me.

I have zero patience so I really rather not fall behind by two weeks on my first attempt. 😅


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OfflineShroomNugget
Mycologist Wannabe
I'm a teapot User Gallery


Registered: 02/10/23
Posts: 355
Last seen: 25 days, 21 hours
Re: Ask Quick Questions, Get Quick Answers [Re: San Pedro Girl]
    #28203965 - 02/25/23 11:47 AM (10 months, 26 days ago)

Quote:

San Pedro Girl said:
Quote:

Weioo said:
Friggin electrical company just ruined my power while fixing others. Place is down to low 50s, high 40s and possibly going to drop below 40.

My 1/2 pint BRF jars started condensating on the inside of the jar walls. Is there a certain temperature that will kill/highly effective my mycelium? I'm staying else where and wondering if I should just box them up and bring them with me.

I have zero patience so I really rather not fall behind by two weeks on my first attempt. 😅



I stall mine at 7°C, so I know at least that is safe for mycelium.




Good to know, thank you sir!


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OfflineShroomNugget
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I'm a teapot User Gallery


Registered: 02/10/23
Posts: 355
Last seen: 25 days, 21 hours
Re: Ask Quick Questions, Get Quick Answers [Re: cf7f36d6]
    #28204638 - 02/25/23 08:29 PM (10 months, 26 days ago)

Sorry for the dumb question but I have no idea where else to ask. What does it take to post in the shroomery sponsor forum? I'm not even able reply to posts and it's very frustrating.


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Edited by ShroomNugget (02/25/23 08:35 PM)


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OfflineShroomNugget
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I'm a teapot User Gallery


Registered: 02/10/23
Posts: 355
Last seen: 25 days, 21 hours
Re: Ask Quick Questions, Get Quick Answers [Re: NFLProof]
    #28204705 - 02/25/23 09:37 PM (10 months, 26 days ago)

Quote:

NFLProof said:
Quote:

Weioo said:
Sorry for the dumb question but I have no idea where else to ask. What does it take to post in the shroomery sponsor forum? I'm not even able reply to posts and it's very frustrating.





https://www.shroomery.org/sponsors.php


Click the contact us link in the first paragraph.





I swear it stated that normal members could respond and reply to posts without needing to be an actual sponsor?


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Edited by ShroomNugget (02/25/23 09:40 PM)


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OfflineShroomNugget
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I'm a teapot User Gallery


Registered: 02/10/23
Posts: 355
Last seen: 25 days, 21 hours
Re: Ask Quick Questions, Get Quick Answers [Re: San Pedro Girl]
    #28206831 - 02/27/23 01:37 PM (10 months, 24 days ago)

Question: I grabbed some single use 10mL inoculation loops. Is it okay to use a single loop to inoculate multiple petri dishes if it's all from the same MSS?


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OfflineShroomNugget
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I'm a teapot User Gallery


Registered: 02/10/23
Posts: 355
Last seen: 25 days, 21 hours
Re: Ask Quick Questions, Get Quick Answers [Re: RastaMind]
    #28206978 - 02/27/23 03:46 PM (10 months, 24 days ago)

Quote:

RastaMind said:
Quote:

Mycovester said:
I am having trouble finding the right test tubes for slants, in a pack that isnt 1000 or 500 peices. I dont ned that many or have the money for that many.

Could I use small PP5 plastic tubs like these but filled 1/2 or 3/4 full of agar?




Howzit! I was going to blow some borosilicate test tubes for mycology work and laser cut some parts for slants in the next week. Can definitely make  extras if you still need.




Hmmm is this a service/product you offer? If so how much for like a dozen slants? I'm in the same boat. :smile:


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Edited by ShroomNugget (02/27/23 04:00 PM)


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OfflineShroomNugget
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Registered: 02/10/23
Posts: 355
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Re: Ask Quick Questions, Get Quick Answers [Re: Vimpat89]
    #28213135 - 03/03/23 08:40 PM (10 months, 20 days ago)

Question: I poured my first agar plates way too hot, at ~140F and quite a few plates ended up with considerable condensation. Now it's been 3-4 days and that condensation has pooled down onto the agar, and many of the plates will continue to spread the water if/when tipped in any way.

I assume that condensation that formed then pooled could be contaminated? Or at the very least make it unsafe/difficult for the plate to thrive? If so are these plates basically DOA or is there hope for them? Do I need to strive to keep the pooled water in one area when I have to move the plates?


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Edited by ShroomNugget (03/03/23 08:43 PM)


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OfflineShroomNugget
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Registered: 02/10/23
Posts: 355
Last seen: 25 days, 21 hours
Re: Ask Quick Questions, Get Quick Answers [Re: myceliation]
    #28213836 - 03/04/23 09:36 AM (10 months, 19 days ago)

Quote:

myceliation said:
Quote:

Weioo said:
Question: I poured my first agar plates way too hot, at ~140F and quite a few plates ended up with considerable condensation. Now it's been 3-4 days and that condensation has pooled down onto the agar, and many of the plates will continue to spread the water if/when tipped in any way.

I assume that condensation that formed then pooled could be contaminated? Or at the very least make it unsafe/difficult for the plate to thrive? If so are these plates basically DOA or is there hope for them? Do I need to strive to keep the pooled water in one area when I have to move the plates?





I'm still learning but this has helped me a ton in dealing with condensation and excess water:
https://www.shroomery.org/forums/showflat.php/Number/27189961




Thank you for a useful answer. ^_^

I'm still looking for answers to my questions related to condensation / contamination though.


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Edited by ShroomNugget (03/04/23 09:38 AM)


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OfflineShroomNugget
Mycologist Wannabe
I'm a teapot User Gallery


Registered: 02/10/23
Posts: 355
Last seen: 25 days, 21 hours
Re: Ask Quick Questions, Get Quick Answers [Re: San Pedro Girl]
    #28214328 - 03/04/23 02:55 PM (10 months, 19 days ago)

Quote:

San Pedro Girl said:
Quote:

Weioo said:
Quote:

myceliation said:
Quote:

Weioo said:
Question: I poured my first agar plates way too hot, at ~140F and quite a few plates ended up with considerable condensation. Now it's been 3-4 days and that condensation has pooled down onto the agar, and many of the plates will continue to spread the water if/when tipped in any way.

I assume that condensation that formed then pooled could be contaminated? Or at the very least make it unsafe/difficult for the plate to thrive? If so are these plates basically DOA or is there hope for them? Do I need to strive to keep the pooled water in one area when I have to move the plates?





I'm still learning but this has helped me a ton in dealing with condensation and excess water:
https://www.shroomery.org/forums/showflat.php/Number/27189961




Thank you for a useful answer. ^_^

I'm still looking for answers to my questions related to condensation / contamination though.



Yes, the condensation could be contaminated. I wouldn’t trash the plate, but when you open it to inoculate, I would carefully pour out any standing water first. The mycelium does not like trying to crawl across water.

You can continue to pour at 140°F/60°C, but stack them and don’t pour too thick to eliminate condensation. Take a look at the link in my signature to see how I pour at that temp.




Ahh, thank you! Unfortunately I had inoculated soon after pouring the agar so the condensation didn't settle until post-incoluation.

I'm aware of the pouring teks/temps but goofed on my first pour from being overly excited and overly fearful of the agar settling. After I poured it I looked it up again and saw pouring should take place at 117-120F, derp. I do stack after pouring but my SAB is the shallow, wide variety so I can only get them stacked like 7 high, thus more top dishes with condensation. >_<


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OfflineShroomNugget
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Registered: 02/10/23
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Re: Ask Quick Questions, Get Quick Answers [Re: Galavant]
    #28215146 - 03/04/23 11:45 PM (10 months, 19 days ago)

Is there any particular reason people mention using 500ml to pour exactly 20 dishes? I've just started agar tek and I've poured about 75 dishes over 3 sessions. Using 100x15 petri Im able to stretch it to almost 30. Is there another reason, like less condensation effect when a dish is 'fuller'?

It seems kinda easy for me to put just enough agar in a dish to almost cover it and then put the top on and give it a quick tilt to one side to finish it off. So I'm wondering if this is taboo or are people just happy with the 20? I'm practicing my tek and placing these in the fridge for my first transfers in a few days. Cling wrapped in stacks of 10 and placed in gallon zip locs.

Thanks in advance!


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Edited by ShroomNugget (03/04/23 11:45 PM)


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OfflineShroomNugget
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Re: Ask Quick Questions, Get Quick Answers [Re: esotericbound]
    #28216022 - 03/05/23 02:43 PM (10 months, 18 days ago)

Question: I'm determining when I should make my first agar xfers after MSS incoluation with a loop. I'm finally beginning to see some small colonies form in a couple areas on some plates.

Should I make transfers as soon as I'm seeing colonization or should I let it grow out a few days and pick the best looking myc then? Most people seem to let plates from out a lot more but I've seen both.

I'm just trying to clean up some plates.


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OfflineShroomNugget
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Registered: 02/10/23
Posts: 355
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Re: Ask Quick Questions, Get Quick Answers [Re: San Pedro Girl]
    #28216196 - 03/05/23 05:01 PM (10 months, 18 days ago)

Thanks fellas!


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OfflineShroomNugget
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Registered: 02/10/23
Posts: 355
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Re: Ask Quick Questions, Get Quick Answers [Re: Eatthedead]
    #28216362 - 03/05/23 06:34 PM (10 months, 18 days ago)

I think full spectrum is best.


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OfflineShroomNugget
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Registered: 02/10/23
Posts: 355
Last seen: 25 days, 21 hours
Re: Ask Quick Questions, Get Quick Answers [Re: dowodenum]
    #28220836 - 03/08/23 02:02 PM (10 months, 15 days ago)

So I've recently recieved spore syringes/swabs from 3 different vendors listed on the sponsors page. Two of three really sell a great product. It almost looks more like a LC than an MSS by color and material within. They also 'work' incredibly well.

The 3rd site has been listed on the sponsor page for over a year now and recently had a celebration with lots of freebies, so I bit. THOSE syringes are more obviously spores in water as I see black specs (verrrrry few) floating. I inoculated some PF tek jars on 2/24 with these and have yet to see  any germination.

What's the difference in syringe/quality/contents and why? It makes me want to avoid vendor #3 :/


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Edited by ShroomNugget (03/08/23 02:03 PM)


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