|
Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 11,144
Loc: Stables
Last seen: 13 minutes, 33 seconds
|
Re: Ask Quick Questions Here [Re: Nichrome]
#27828282 - 06/20/22 06:40 AM (1 year, 7 months ago) |
|
|
Quick question:
If none of my agar is performing from spore, LC, or live myc, what’s the most likely problem? It is MEA with a 8:8:400 malt:agar:water ratio.
|
Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 11,144
Loc: Stables
Last seen: 13 minutes, 33 seconds
|
Re: Ask Quick Questions Here [Re: Rotnpins]
#27828609 - 06/20/22 12:10 PM (1 year, 7 months ago) |
|
|
Correct, sorry. It’s 8g/8g/400ml agar/malt/water. Just sorta the universal beginner recipe here on the forum.
I have my plates in the incubator with my spawn. They’ll grow a little then stall. I’ll keep trying with better samples as I get a grow going, but if there’s anything off the top of your heads you think might be a problem I’d love to know. Otherwise I’ll continue to collect data and come back in a couple of months.
|
Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 11,144
Loc: Stables
Last seen: 13 minutes, 33 seconds
|
Re: Ask Quick Questions Here [Re: Rotnpins] 1
#27829128 - 06/20/22 07:02 PM (1 year, 7 months ago) |
|
|
Quote:
Rotnpins said: Have any pictures?
I didn’t see this reply, my bad.
Here’s the one that got the most growth from spore. It was thin, translucent, and hasn’t changed in I guess a week or so?

Edit: as you can see by the sharpie, this is also made from a dirty LC. It’s the only one of its kind I made, so it might not be the best example. Others are spore and one is a colonized grain. I can take pics of those if that would be helpful, but I can also just let them go for a few weeks and see if they do anything.
Edited by Smellyhobbit (06/20/22 07:07 PM)
|
Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 11,144
Loc: Stables
Last seen: 13 minutes, 33 seconds
|
|
And I’ll put my dishes in the cabinet if room temp is optimal and see if anything changes. I’m going to continue making more plates, since I have the materials. It’ll help refine my process anyway. In a couple of months I’ll do a write up and start a new thread if problems persist.
Thanks!
|
Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 11,144
Loc: Stables
Last seen: 13 minutes, 33 seconds
|
Re: Ask Quick Questions Here [Re: milkboy]
#27829158 - 06/20/22 07:20 PM (1 year, 7 months ago) |
|
|
Quote:
milkboy said: That plate is contaminated, looks like the bacteria won the battle. RIP
Nothing wrong with your agar recipe
Ok that’s good to know. I’m not leaning on agar for results atm, but I would obviously like to get it going to refine my genetics and ensure clean spawn. This was dirty LC, and I thought I might get away with doing a couple of transfers and cleaning it. Oh well!
I have spores galore and Petri dishes aplenty. No harm done.
|
Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 11,144
Loc: Stables
Last seen: 13 minutes, 33 seconds
|
Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
#27831443 - 06/22/22 08:44 AM (1 year, 7 months ago) |
|
|
Quick question:
People dunk and roll PF cakes after the first flush. Is there any benefit to adding a layer of verm to a shoebox tub after floating and harvesting?
|
Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 11,144
Loc: Stables
Last seen: 13 minutes, 33 seconds
|
Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome] 1
#27834051 - 06/24/22 07:25 AM (1 year, 7 months ago) |
|
|
Do prints need to be kept in the fridge? Is that where people normally keep them? I haven’t taken any yet, but my mind defaulted to room temp for some reason.
|
Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 11,144
Loc: Stables
Last seen: 13 minutes, 33 seconds
|
Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome] 1
#27837651 - 06/26/22 04:05 PM (1 year, 6 months ago) |
|
|
It’s a shame. That looks like it had an excellent pin set.
|
Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 11,144
Loc: Stables
Last seen: 13 minutes, 33 seconds
|
|
If they are sterile, which they should be, then they’ll last months in the fridge or at room temp. They’ll last longer in the fridge - they’ll dry out more slowly. But if they’ve been poured and sterilized correctly, you won’t see contams from them sitting around.
|
Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 11,144
Loc: Stables
Last seen: 13 minutes, 33 seconds
|
Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome] 1
#27842005 - 06/29/22 08:31 AM (1 year, 6 months ago) |
|
|
Quick question:
Once a contam like trich presents itself in a tub, should the entirety of the tub no longer be considered a candidate for tissue sampling? It started pinning yesterday, and this morning trich showed up like a magician put it there. I’ve isolated the tub and I’m going to let it do it’s thing. I’m sure it’s a lost cause, but would you recommend taking a pin and putting it to agar or wait for another opportunity?
|
Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 11,144
Loc: Stables
Last seen: 13 minutes, 33 seconds
|
Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome] 1
#27842022 - 06/29/22 08:48 AM (1 year, 6 months ago) |
|
|
Ok excellent. I’ll take a pin today and put it to agar. Hopefully this one works. Haven’t had success with agar yet. It moves really really slow and then stops. Transfers never do anything. I’m thinking a pin has a lot higher chance of success
|
Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 11,144
Loc: Stables
Last seen: 13 minutes, 33 seconds
|
Re: Ask Quick Questions, Get Quick Answers [Re: rumfor69]
#27842026 - 06/29/22 08:52 AM (1 year, 6 months ago) |
|
|
Quote:
rumfor69 said: What's the agar and recipe you're using?
I’m using the 20g/20g/1000ml malt/agar/water. None of my pours have been contaminated, which is great, but the growth is just not happening. I’m sure I’m doing something wrong, maybe even being impatient, but I’m coming up on 3 weeks and none of it is even the size of a dime, including spore streaks and live mycelium from a piece of grain.
Edit: sorry it’s not 10g of each dry ingredient, it’s 20g. Sleepy math.
Edited by Smellyhobbit (06/29/22 08:57 AM)
|
Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 11,144
Loc: Stables
Last seen: 13 minutes, 33 seconds
|
Re: Ask Quick Questions, Get Quick Answers [Re: Guerrilla]
#27842033 - 06/29/22 08:56 AM (1 year, 6 months ago) |
|
|
Quote:
Guerrilla said:
Quote:
Smellyhobbit said:
Quote:
rumfor69 said: What's the agar and recipe you're using?
I’m using the 10g/10g/1000ml malt/agar/water. None of my pours have been contaminated, which is great, but the growth is just not happening. I’m sure I’m doing something wrong, maybe even being impatient, but I’m coming up on 3 weeks and none of it is even the size of a dime, including spore streaks and live mycelium from a piece of grain.
Sounds like maybe bacteria is present and preventing growth.
That’s what I was thinking was the most probable cause, especially with the mycelium I’ve cultured, since it wasn’t from a clean source. Is it usually difficult to clean spore syringes? I have some that are growing, they’re just growing very very slowly.
Edited by Smellyhobbit (06/29/22 08:58 AM)
|
Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 11,144
Loc: Stables
Last seen: 13 minutes, 33 seconds
|
Re: Ask Quick Questions, Get Quick Answers [Re: rumfor69]
#27842041 - 06/29/22 09:04 AM (1 year, 6 months ago) |
|
|
Quote:
rumfor69 said: I'm thinking bacteria also cause if it was mold it'd visibly contaminating the dish.
You using a flowhood or SAB?
Could also just be your technique. Are you cooling tools down or letting them cool down all the way before touching them to tissue or spores?
SAB, minimized airflow in apartment for about 30 min beforehand and a spray of Lysol. All work surfaces and tools sprayed/wiped with 70% iso and allowed to dry. SAB wiped with iso and allowed to dry. I use scalpels that are individually sealed, handled with washed then gloved then iso’d hands. I flame the scalpel then press it into the agar to cool it, which is shown in one of the guides here as an acceptable method of cooling. I cut a small piece and transfer it mycelium down to a new dish, being careful not to open it until that moment, not hover my hand over either plate, and then quickly lid it. Label and wrap with an inch wide piece of saran wrap. Then it goes into the cabinet with my grows on a separate shelf with the same light cycle and temps as the others.
I think that’s my whole process. Do you think the tools are too hot and damaging the mycelium when I transfer them?
|
Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 11,144
Loc: Stables
Last seen: 13 minutes, 33 seconds
|
|
Quote:
CowsPoopShrooms said:
Quote:
Smellyhobbit said: Then it goes into the cabinet with my grows on a separate shelf with the same light cycle and temps as the others.
Everything else is growing ok? It is only an agar issue?
Well it’s growing quickly. I’m obviously dealing with contamination issues since I don’t have a clean culture yet, but speed is not an issue with anything else. Even bacterial contam doesn’t really slow anything down. It’s trich and other stuff that’s getting tubs yeeted.
|
Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 11,144
Loc: Stables
Last seen: 13 minutes, 33 seconds
|
Re: Ask Quick Questions, Get Quick Answers [Re: Rotnpins] 1
#27842108 - 06/29/22 10:16 AM (1 year, 6 months ago) |
|
|
Quote:
Rotnpins said: I was dealing with a bunch of plates stalling, I thought it might have been my agar recipe because I couldn't see any visible contam... just healthy looking mycelium that grew for about a week and then stopped...
I ended up trying a different agar recipe and moving on,.. but when I went to look at the stalled plates again a couple weeks later, I could now see some sort of contamination growing.. light whispy growth.. thinking it was some type of mold I can post some pictures of the plates at different stages when i get home so you can compare... I'd recommend holding on to your stalled plates and checking back on them in a few weeks to see of the contamination has become more visable
I have a riptide plate that I’m 99% sure is showing some signs of mold. I’m guessing it’s gotta be contam issues, which sucks because I’m not sure how I’m meant to clean them up if they don’t grow at least a transferable section. I’m not going to toss them. I’m sure there’s valuable knowledge to be had. The quest for clean culture continues.
|
Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 11,144
Loc: Stables
Last seen: 13 minutes, 33 seconds
|
Re: Ask Quick Questions, Get Quick Answers [Re: albird71] 1
#27842117 - 06/29/22 10:23 AM (1 year, 6 months ago) |
|
|
Golden teachers are known for their pointed caps. Not all golden teachers have the point, but if one tub is pointed and the other isn’t, the pointed one is almost definitely GT. Congrats on your harvest.
|
Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 11,144
Loc: Stables
Last seen: 13 minutes, 33 seconds
|
|
Quote:
Oneleggedwarrior said:
Quote:
Smellyhobbit said: If they are sterile, which they should be, then they’ll last months in the fridge or at room temp. They’ll last longer in the fridge - they’ll dry out more slowly. But if they’ve been poured and sterilized correctly, you won’t see contams from them sitting around.
Thank-you, have thankfully poured and no contam signs 48 hours post sterilisation
I’ve been recommended to leave them at room temp for about a week just to make sure things don’t grow on them. Then put them in the fridge for longevity.
|
Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 11,144
Loc: Stables
Last seen: 13 minutes, 33 seconds
|
Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome] 6
#27842752 - 06/29/22 06:20 PM (1 year, 6 months ago) |
|
|
Just mix em all together and call it trail mix :P
|
Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 11,144
Loc: Stables
Last seen: 13 minutes, 33 seconds
|
Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome] 2
#27844707 - 07/01/22 08:14 AM (1 year, 6 months ago) |
|
|
Quick question: how long can hydrated coir stay good in a sealed bucket?
|
|