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 Judge Doom
Registered: 12/19/20
Posts: 263
Loc: Arizona Bay
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 Re: APE/Ps. natalensis hybrid  [Re: DERRAYLD]
#27772339 - 05/11/22 07:30 AM (1 year, 11 months ago) |
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Of course! It ended up being a really nice pic so I thought I'd share.
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mind.at.large
Myconerd
Registered: 12/13/16
Posts: 1,229
Loc: Floating in liquid gardens
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Re: APE/Ps. natalensis hybrid [Re: Judge Doom]
#27773212 - 05/11/22 09:17 PM (1 year, 10 months ago) |
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Great job dude! This is awesome!
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Mind's Easy Bag 2 Bag Grain Transfers
Endless Sub Tek
...the doll's trying to kill me and the toaster's been laughing at me...
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Judge Doom
Registered: 12/19/20
Posts: 263
Loc: Arizona Bay
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Thanks!
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Judge Doom
Registered: 12/19/20
Posts: 263
Loc: Arizona Bay
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Re: APE/Ps. natalensis hybrid [Re: Judge Doom]
#27773284 - 05/11/22 10:08 PM (1 year, 10 months ago) |
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 Natedogg97
Patch reaper
Registered: 11/10/17
Posts: 234
Loc: Oregon
Last seen: 1 day, 1 hour
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Re: APE/Ps. natalensis hybrid [Re: Judge Doom]
#27773498 - 05/12/22 04:01 AM (1 year, 10 months ago) |
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UnbornCaius
Hobbyist
Registered: 04/20/22
Posts: 45
Last seen: 1 year, 10 months
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Re: APE/Ps. natalensis hybrid [Re: Judge Doom]
#27773553 - 05/12/22 06:33 AM (1 year, 10 months ago) |
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Wow, this is super cool. Excellent work
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Rainy21
Stranger
Registered: 11/01/21
Posts: 9
Last seen: 3 months, 25 days
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Re: APE/Ps. natalensis hybrid [Re: UnbornCaius]
#27782980 - 05/18/22 12:59 PM (1 year, 10 months ago) |
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Loving the purplish hue that some of the caps have
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holybrown101
Stranger
Registered: 11/01/09
Posts: 150
Last seen: 10 months, 21 days
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Re: APE/Ps. natalensis hybrid [Re: Rainy21] 1
#27783752 - 05/19/22 02:05 AM (1 year, 10 months ago) |
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Makes me want to pickup a microscope and experiment using electrical fields to hybridize species.
I'm really interested in cross species hybridization.
I really want to see a pan cyan /cube hybrid.
I have a theory about bad genetics such as penis envy and how by correcting its genetics with a mutant / different species readily hybridize or with an electrical field would make it easier.
I'm seriously thinking about making the leap! This thread rocks!!!
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psillyboy
Florida man
Registered: 09/26/14
Posts: 582
Loc: In the 60's
Last seen: 20 hours, 27 minutes
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Re: APE/Ps. natalensis hybrid [Re: Judge Doom]
#27784044 - 05/19/22 09:25 AM (1 year, 10 months ago) |
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--------------------
"True terror is to wake up one morning and discover that your high school class is running the country - K. Vonnegut
“The real truth, that dare not speak itself, is that no one is in control. Absolutely no one.” ― Terence McKenna
"LSD is a psychedelic drug which occasionally causes psychotic behavior in people who have never taken it." - Timothy Leary
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mycorry
The Empirical
Registered: 06/01/18
Posts: 387
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Re: APE/Ps. natalensis hybrid [Re: psillyboy] 1
#27784099 - 05/19/22 10:31 AM (1 year, 10 months ago) |
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Ok ok, now hybridize the other two close relatives of cubes: psilocybe indica and psilocybe chuxiongensis. Then hybridize the results with your cube/nat hybrid. Spend the time to stabilize and....
You have invented:
PSILOCYBE PANGEA
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My Trade Lists
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smalltalk_canceled
Babnik
Registered: 07/13/20
Posts: 2,904
Last seen: 1 day, 1 hour
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Re: APE/Ps. natalensis hybrid [Re: Judge Doom]
#27784576 - 05/19/22 04:29 PM (1 year, 10 months ago) |
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thats pretty rad dude, i remember posting about my own attempts to cross natal and ape by just mixing grains but you obviously went a faster and better way. Great work!!
What I did was clone this fruit, which I believe to be some kind of cross:
The white one actually turns into this, from a very APE pheno to something different. Seriously it changed colour/pigment.
shroomery creating a new species,? does this qualify?
how to measure beyond visual look...
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Willpower is the one true virtue
   
Edited by smalltalk_canceled (05/19/22 04:31 PM)
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Judge Doom
Registered: 12/19/20
Posts: 263
Loc: Arizona Bay
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Re: APE/Ps. natalensis hybrid [Re: mycorry] 2
#27784618 - 05/19/22 05:00 PM (1 year, 10 months ago) |
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I now have monokaryons of Ps. pseudoaztecorum and Ps. indica so I will do just that! Pairing them up in about a week and I'll let you know how it goes. I'm assuming they will be compatible.
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Judge Doom
Registered: 12/19/20
Posts: 263
Loc: Arizona Bay
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Quote:
smalltalk_canceled said:
thats pretty rad dude, i remember posting about my own attempts to cross natal and ape by just mixing grains but you obviously went a faster and better way. Great work!!
What I did was clone this fruit, which I believe to be some kind of cross:
The white one actually turns into this, from a very APE pheno to something different. Seriously it changed colour/pigment.
shroomery creating a new species,? does this qualify?
how to measure beyond visual look...
I do not believe it is possible to cross dikaryotic strains in any compacity, including mixing grain together. What you need is monokaryons and then to confirm compatibility with clamp connections. Spontaneous dedikaryotization is very rare, and probably even more rare with cultures on favorable substrates like grain. But there's always exceptions and surprises. I wouldn't call that a hybrid because you just can't satisfy the burden of proof but it is certainly possible for cubensis and natalensis to hybridize, as I have shown.
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Some other guy
Grower of Mold
Registered: 09/06/21
Posts: 273
Last seen: 9 months, 29 days
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Re: APE/Ps. natalensis hybrid [Re: Judge Doom]
#27806855 - 06/05/22 11:00 AM (1 year, 10 months ago) |
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What are the colonizing times like on these?
Are you growing them on straight coir, cvg, poo?
Do they need to be cased to help with blobs?
Last but not least, potency. =)
Very very cool project and I can't wait to see how it turns out.
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Judge Doom
Registered: 12/19/20
Posts: 263
Loc: Arizona Bay
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Quote:
Some other guy said:
What are the colonizing times like on these?
Are you growing them on straight coir, cvg, poo?
Do they need to be cased to help with blobs?
Last but not least, potency. =)
Very very cool project and I can't wait to see how it turns out.
The colonizing times are very slow, almost twice as slow. Very much like APE.
And no there is no blobs or casing.
Yeah these are on straight cc/v.
And the potency is great, I really enjoyed the ride.
Thanks man! I'll post updates when I can.
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Edited by Judge Doom (07/14/22 11:48 AM)
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Guerrilla
Bumbaclart
Registered: 01/30/21
Posts: 3,170
Loc: United Kingdom 
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Re: APE/Ps. natalensis hybrid [Re: Judge Doom]
#27807054 - 06/05/22 01:52 PM (1 year, 10 months ago) |
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Quote:
Judge Doom said:
I do not believe it is possible to cross dikaryotic strains in any compacity, including mixing grain together. What you need is monokaryons and then to confirm compatibility with clamp connections. Spontaneous dedikaryotization is very rare
I'd be interested in a break down of your process. Are there any threads detailing what you went through? If not perhaps you would care to write one some day? A picture guide on mating spores / strain crossing with a scope would get a lot of attention.
You've greatly piqued my interest - I'm into the idea of getting a microscope more than ever now. Which one do you have?
Edited by Guerrilla (06/05/22 01:56 PM)
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DEZn00ts
The Wizard of The Midwest
Registered: 01/17/13
Posts: 389
Loc: Anus, Cantaffordit
Last seen: 3 months, 29 days
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Re: APE/Ps. natalensis hybrid [Re: Guerrilla]
#27808769 - 06/06/22 07:22 PM (1 year, 10 months ago) |
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Man them bitches look cool as hell! Fat penis head with the thinner straight stem. THe color of the caps are pretty unique too. Very cool.
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"it's genetic. Asians are better at math if born in America or Asia. Black people run faster born in Africa or America. White people are still more racist no matter where they were born either. it's genetic." -bodhisatta
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Judge Doom
Registered: 12/19/20
Posts: 263
Loc: Arizona Bay
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Re: APE/Ps. natalensis hybrid [Re: Guerrilla] 2
#27825937 - 06/18/22 03:01 PM (1 year, 9 months ago) |
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Quote:
Guerrilla said:
Quote:
Judge Doom said:
I do not believe it is possible to cross dikaryotic strains in any compacity, including mixing grain together. What you need is monokaryons and then to confirm compatibility with clamp connections. Spontaneous dedikaryotization is very rare
I'd be interested in a break down of your process. Are there any threads detailing what you went through? If not perhaps you would care to write one some day? A picture guide on mating spores / strain crossing with a scope would get a lot of attention.
You've greatly piqued my interest - I'm into the idea of getting a microscope more than ever now. Which one do you have?
I will work on giving a visual outline in the future but for now I'll just give you a quick run down here. I germinated APE spores and isolated the monokaryons using my isolation technique, then my friend Yoshi Amano germinated Ps. natalensis spores and also isolated them using my technique. He handed me the natalensis monokaryons and I paired them with 16 of my cubensis monokaryons in some multiwell culture plates (48). I did a total of 144 pairings. After they had some time to colonize the wells I took small samples of the mycelium with a scalpel and checked the hyphae for clamp connections. Once I confirmed the presence of clamp connections I isolated the pairings in petris and then fruited them.
I'm glad you find it as fascinating as I do! I use a Amscope B120 compound microscope.
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Edited by Judge Doom (06/18/22 03:32 PM)
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Judge Doom
Registered: 12/19/20
Posts: 263
Loc: Arizona Bay
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Re: APE/Ps. natalensis hybrid [Re: DEZn00ts] 1
#27825940 - 06/18/22 03:07 PM (1 year, 9 months ago) |
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Quote:
DEZn00ts said:
Man them bitches look cool as hell! Fat penis head with the thinner straight stem. THe color of the caps are pretty unique too. Very cool.
Hell yeah! I thought so too. And it's definitely awesome seeing the APE phenotypes in the cap matched with the prominent natalensis phenotypes. Thank you!
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Psilosophy23
Psychonaught
Registered: 06/18/22
Posts: 27
Last seen: 1 year, 30 days
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Re: APE/Ps. natalensis hybrid [Re: Judge Doom]
#27826111 - 06/18/22 05:28 PM (1 year, 9 months ago) |
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This is nuts, so basically that is a species hybrid right ?
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