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Bab.D
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Registered: 09/06/17
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Agar plates of my clone fruited. How to proceed?
#27819184 - 06/14/22 11:36 AM (2 years, 7 months ago) |
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So a couple of my agar plates with my clone samples fruited for some reason. How to proceed?
Should I clone from the agar or the shroom bodies? If the second, which ones should be taken?
One of the plates had contamination and I already cloned it into another coupled of dishes previously, but decided to keep the plate to see how the mycelium would cope with the mold . It has the biggest fruit btw.


Any help will be appreciated.
The clone is from my first shroom ever that grew 20g wet in just 2 days way before the rest of the flush.
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nektar61
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Re: Agar plates of my clone fruited. How to proceed? [Re: Bab.D]
#27819203 - 06/14/22 11:51 AM (2 years, 7 months ago) |
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FYI, no problem, but I'm not sure this qualifies as Advanced Mycology. Should probably be posted in Agar Envy or just general cultivation. (You'd get an answer quicker regardless on Agar Envy though if I hadn't seen this on the front page.)
If you post follow ups I'd post them in Agar Envy.
Cool looking plates.
Wait until they grow to plins (plate pins) with tiny caps, but before they open the caps and drop spores. Actually there's already one almost ready on the first plate in the top left.
You can actually clone them before that, but if they have caps it's easier to see which one is performing best, also easier to not get mycelium along with it.
Find the fattest, tallest, or fastest growing one and transfer to a new plate (or transfer all 3 to different plates).
Use flame-sterilized scalpel. Cut them off above the base so you don't bring any non-clone mycelium, then drop it in a fresh plate, and use the scalpel to push it a little way into the plate.
That will grow out to clone mycelium. Transfer that, then when clean, send to grain.
Read this: https://www.shroomery.org/forums/showflat.php/Number/27819173#27819173
Also read the "things I wish I'd known" link in my sig line. There's more in there on cloning.
What's the brown spot in the bottom left of the top plate?
Kat
Edited by nektar61 (06/14/22 12:09 PM)
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Bab.D
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Re: Agar plates of my clone fruited. How to proceed? [Re: nektar61] 1
#27819233 - 06/14/22 12:21 PM (2 years, 7 months ago) |
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Thanks for the answer. Could an admin move this to general cultivation? To avoid double posting. 
I saw some comments on reddit about the need of using a syringe for this type of cloning, so I wasn´t sure that was a regular technique, sorry for the confusion!
The spot is some mold that grew alongside the mycelium, but when I saw it the mycelium had already started growing on top of it, so I took a couple of samples from the best looking strands of the unaffected area, and left the plate to see if the mycelium would win the battle and just check out how things would go from there.
Will definitely check the links, thanks!
Edited by Bab.D (06/14/22 12:22 PM)
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nektar61
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Re: Agar plates of my clone fruited. How to proceed? [Re: Bab.D]
#27819250 - 06/14/22 12:32 PM (2 years, 7 months ago) |
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Quote:
Bab.D said: Thanks for the answer. Could an admin move this to general cultivation? To avoid double posting. 
I saw some comments on reddit about the need of using a syringe for this type of cloning, so I wasn´t sure that was a regular technique, sorry for the confusion!
The spot is some mold that grew alongside the mycelium, but when I saw it the mycelium had already started growing on top of it, so I took a couple of samples from the best looking strands of the unaffected area, and left the plate to see if the mycelium would win the battle and just check out how things would go from there.
Will definitely check the links, thanks!
Reddit is NOT a place to take advice.
As I say in my sig line post:
Don't take grow advice from Reddit. It's a fun place for some to post and be hyper, but there is so much wrong advice there it's crazy. I've seen people tell someone "That grow is contamed" when it's not, and seen people throw out a great grow. That would get someone banned for life here, and that's a good thing. "Reddit shroom subs: where you get yelled at for being a gatekeeper if you suggest using agar.
"Reddit will give 5 upvotes to proper advice, 50 upvotes to wrong advice, and 500 upvotes to a shroom that looks like a butt."
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nektar61
Heterotroph



Registered: 07/04/20
Posts: 3,687
Loc: Hamsterdam
Last seen: 49 seconds
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Re: Agar plates of my clone fruited. How to proceed? [Re: Bab.D]
#27819283 - 06/14/22 12:51 PM (2 years, 7 months ago) |
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If you want this moved to Mushroom Cultivation, click on the "notify moderator" at the bottom of the top post (the whistle icon) and ask them to move it.
Edited by nektar61 (06/14/22 01:14 PM)
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Pastywhyte
Say hello to my little friend


Registered: 09/15/12
Posts: 37,972
Loc: Canada
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Re: Agar plates of my clone fruited. How to proceed? (moved) [Re: Bab.D] 1
#27820720 - 06/15/22 11:58 AM (2 years, 6 months ago) |
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This thread was moved from Advanced Mycology.
Reason: Better fit here
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