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redcrow

Registered: 03/25/22
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Transfers from this plate?
#27816377 - 06/12/22 04:27 PM (2 years, 7 months ago) |
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What do you think? I don't think this plate is ready to be used as an inoculant. Where would you take samples from to try and clean this up a bit?

Thanks for your input.
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LadysKnight
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Re: Transfers from this plate? [Re: redcrow]
#27816432 - 06/12/22 04:57 PM (2 years, 7 months ago) |
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Looks mold enmeshed, I would not try to clean it.
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grownright
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Re: Transfers from this plate? [Re: redcrow]
#27816434 - 06/12/22 04:58 PM (2 years, 7 months ago) |
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Basically anywhere around that bacterial spot. The culture looks good but its still a transfer away from being ready
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grownright
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Re: Transfers from this plate? [Re: LadysKnight]
#27816438 - 06/12/22 04:59 PM (2 years, 7 months ago) |
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Quote:
LadysKnight said: Looks mold enmeshed, I would not try to clean it.
You think so? I guess those wispier areas where theres a break in the myc do look a lil sus
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LadysKnight
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Re: Transfers from this plate? [Re: grownright]
#27816449 - 06/12/22 05:10 PM (2 years, 7 months ago) |
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Quote:
grownright said:
Quote:
LadysKnight said: Looks mold enmeshed, I would not try to clean it.
You think so? I guess those wispier areas where theres a break in the myc do look a lil sus
That's where I see it most, yes.
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TheTimelessDon
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Re: Transfers from this plate? [Re: redcrow]
#27816469 - 06/12/22 05:24 PM (2 years, 7 months ago) |
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That's an interesting growth pattern... Looks like a ghost came in and did transfers off of what would have normally been a perfect round plate, lol.
I'd transfer sections between each of the splits...and honestly, I'd put the entire left side to a small jar of grain and see how it likes it But this is just a grain of salt, food for thought, response... Because I'm just happy to see you moving myc around and getting good growth, so I'm anxious to see you get some good looking fruit pics!
LK might be right, so when you do anything with this plate, make sure to take a piece of the fuzz growing between the sections and put it under your microscope and confirm for the classroom. I don't know how to advise moving forward if it is...
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Nichrome
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Can someone please show me what "enmeshed mold" is? I have honestly never seen it happen.
That is sectoring. That type of sectoring is indicative of a colony with internal competition going on. Like some of the hyphae are planning a mutiny or like a case of star bellied sneetches vs. regular sneetches. Possibly just a genetic defect. Long story short those patterns often lead to susceptibility to mold taking over but there is no mold in that culture.
Definitely not ready as inoculant with that little bacteria booger in there. More transfers.
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TheTimelessDon
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Re: Transfers from this plate? [Re: Nichrome]
#27816586 - 06/12/22 06:28 PM (2 years, 7 months ago) |
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Quote:
Nichrome said: Like some of the hyphae are planning a mutiny or like a case of star bellied sneetches vs. regular sneetches.
Awesome!
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LadysKnight
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Re: Transfers from this plate? [Re: Nichrome]
#27816598 - 06/12/22 06:34 PM (2 years, 7 months ago) |
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Quote:
Nichrome said: Can someone please show me what "enmeshed mold" is? I have honestly never seen it happen.
That is sectoring. That type of sectoring is indicative of a colony with internal competition going on. Like some of the hyphae are planning a mutiny or like a case of star bellied sneetches vs. regular sneetches. Possibly just a genetic defect. Long story short those patterns often lead to susceptibility to mold taking over but there is no mold in that culture.
Definitely not ready as inoculant with that little bacteria booger in there. More transfers.
I'll try. Immediately, the plate looks blurry and out of focus, but it's not the focus. That bacteria colony at the bottom is clear and crisp, as is the table and wire rack. The mycelium is blurred by the mold that can be seen in the missing wedges of mycelium around the edges and in the clear halo of mold surrounding the culture. The only way to prove it is to let it grow, but this is what I'm seeing.
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Nichrome
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Re: Transfers from this plate? [Re: LadysKnight]
#27816788 - 06/12/22 09:05 PM (2 years, 7 months ago) |
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I have looked at those weak spots in sectoring like that extensively with a microscope as well as transferred those areas and so on, and I have yet to find "mold" in a singe one of them out of maybe hundreds that look like that. You are describing "symptoms" of an old wives tale but not really convincing me there is something there that is not. Up close under a microscope those missing areas are still just P. cubensis mycelium. It is just a growth pattern.
I have grown a lot of cultures that had mold. I grow many types of mold on purpose and enjoy cleaning up wild cultures and do so regularly. I have yet to see "enmeshed" mold in a P. Cubensis culture.
If this does indeed happen I really would love to know about it and would love to work with some of the stuff. I really would.
Getting mold from a culture like that in the end only "proves" susceptibility to mold, not that there is mold already there riding along. Trichoderma and the things called "cobweb" molds are extremely visible with a microscope. Trichoderma of any kind does not enmesh with P. cubensis mycelium.
The enmeshed mold idea is really old hat.
This is mold germinating from some dirty Natalensis spores.
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LadysKnight
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Re: Transfers from this plate? [Re: Nichrome]
#27816817 - 06/12/22 09:21 PM (2 years, 7 months ago) |
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Your luck is better than mine because I have dealt with plenty of enmeshed mold, grown it for months , and not for fun. I can see the mold on the plate. Let it grow.
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Nichrome
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Re: Transfers from this plate? [Re: LadysKnight] 1
#27816822 - 06/12/22 09:24 PM (2 years, 7 months ago) |
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What kind of mold do you see? What kind of mold do you get from cultures that look like this?
I don't see any mold.
Here is an example of a culture that is not moldy but sectoring in a similar way. The first picture is at the edge where the thick stuff meets the thin stuff. You can see that it is the same mycelium and that plenty of hyphae are meeting and joining between the two. The thin stuff is the same mycelium just expressing a slightly different growth pattern. The second photo is the thin area and the third is the plate photographed. These photos were from just now.
Edited by Nichrome (06/12/22 09:41 PM)
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Boozie
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Re: Transfers from this plate? [Re: Nichrome]
#27816836 - 06/12/22 09:39 PM (2 years, 7 months ago) |
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Anywhere from 6-12 o’clock looks like prime transfer pickins’ to me. Stay away from the spotty runaway areas and that little “bacterial booger” Nichrome pointed out. More clean up is needed for sure, but you should be fine.
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LadysKnight
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Re: Transfers from this plate? [Re: Nichrome]
#27816851 - 06/12/22 09:53 PM (2 years, 7 months ago) |
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This is the 4oclock section, the little white puffy clouds that are disconnected from the rest of the mass. IME, this is mold.
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Nichrome
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Re: Transfers from this plate? [Re: LadysKnight]
#27816852 - 06/12/22 09:54 PM (2 years, 7 months ago) |
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If you were to look up close you'd see it is the same species of mycelium.
Another example of the same type of sectoring that is not mold.
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Edited by Nichrome (06/12/22 09:56 PM)
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LadysKnight
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Re: Transfers from this plate? [Re: LadysKnight]
#27816856 - 06/12/22 10:00 PM (2 years, 7 months ago) |
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Hmmm. Ok if that is true, and cube mycelium can look like disorganized wispy threads. And it's not mold but is disposed to mold. Then idkwtf a clean plate looks like.
Tougher call on your example, it's kinda blurry and out of focus.
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Edited by LadysKnight (06/12/22 10:06 PM)
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Nichrome
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Re: Transfers from this plate? [Re: LadysKnight]
#27816871 - 06/12/22 10:11 PM (2 years, 7 months ago) |
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Susceptibility to mold gives the same result as if the mold were riding along which is failure and a moldy finish.
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cozmyc
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Re: Transfers from this plate? [Re: redcrow]
#27816873 - 06/12/22 10:15 PM (2 years, 7 months ago) |
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In my few experiences with mold, it usually doesn't grow at the same rate as myc. It soon overtakes and grows faster.
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LadysKnight
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Re: Transfers from this plate? [Re: Nichrome]
#27816875 - 06/12/22 10:19 PM (2 years, 7 months ago) |
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Maybe op can take micropics?
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Eggsalts
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Re: Transfers from this plate? [Re: cozmyc]
#27817119 - 06/13/22 05:54 AM (2 years, 7 months ago) |
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I’ve had the opposite experience with plates that looked like this. If I have a nasty plate like this I transfer it to a low nute plate untill I see that little jetting of ropes come off the transfer, and the second I see them I just transfer it straight to a high nute plate. Seems to outrun the contaminant just fine.
The explanation of the competing mycelliums causing that weird growth helped put some shit together for me… thanks.
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LadysKnight
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Re: Transfers from this plate? [Re: Eggsalts]
#27817199 - 06/13/22 07:52 AM (2 years, 7 months ago) |
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Hopefully op will continue working with this culture and we will learn something.
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redcrow

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Re: Transfers from this plate? [Re: redcrow]
#27817296 - 06/13/22 09:19 AM (2 years, 7 months ago) |
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My take away from all this is it's a toss up whether this plate is worth further culturing. As I stated at the start, it's the result of no less than 3 attempts at cleaning it up and it started from a questionable source.
With that in mind, I think I'm going to abandon the effort. It's been worthwhile for the experience and it has improved my skills. But I've got a whole stack of ready to inoculate plates and two very good looking spore prints from a known to be reliable vendor. So I'm going to channel my work down that path and see what happens.
In my experience, persistence is a bit like gravity. It always wins.
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rumfor69
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Re: Transfers from this plate? [Re: redcrow] 1
#27817324 - 06/13/22 09:43 AM (2 years, 7 months ago) |
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I'd still take a couple transfers from that 6-12 o'clock position just to see what happens. It'd be a shame to toss out what could become a good clean culture.
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LadysKnight
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Re: Transfers from this plate? [Re: rumfor69]
#27817369 - 06/13/22 10:15 AM (2 years, 7 months ago) |
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I learned that I'm not seeing enmeshed mold, instead I'm seeing the future and can tell when a clean culture will spontaneously develop moldy spawn.
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redcrow

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Re: Transfers from this plate? [Re: redcrow] 1
#27817371 - 06/13/22 10:20 AM (2 years, 7 months ago) |
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Oh, what the hell... Making one more transfer wouldn't exactly be a labor intensive task. I've got some inoculations to do with those new spore prints so I might as well do that at the same time. Like I said, persistence.
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milkboy
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Re: Transfers from this plate? [Re: LadysKnight] 2
#27817383 - 06/13/22 10:27 AM (2 years, 7 months ago) |
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I would just like to add Ive had many people tell me plates were enmeshed with mold, never found it to be true, always mycelium.
This APE plate for example, did one additional transfer and sent to 1qt of grain, produced 6 flushes almost 2 ozs dry.
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Nichrome
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Re: Transfers from this plate? [Re: rumfor69] 1
#27817530 - 06/13/22 12:13 PM (2 years, 7 months ago) |
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Quote:
rumfor69 said: I'd still take a couple transfers from that 6-12 o'clock position just to see what happens. It'd be a shame to toss out what could become a good clean culture.
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Boozie
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Re: Transfers from this plate? [Re: Nichrome]
#27818578 - 06/13/22 09:54 PM (2 years, 7 months ago) |
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Quote:
Nichrome said:
Quote:
rumfor69 said: I'd still take a couple transfers from that 6-12 o'clock position just to see what happens. It'd be a shame to toss out what could become a good clean culture.

Seconded.
Bet dollars to donuts that plate, while not totally clean by any means, will transfer out just fine. …Where did this “enmeshed mold” term come from, anyway? Mold is not going to just tag along and grow at the exact same pace as the healthy mycelium. It will overtake very quickly, halt healthy growth and cause very obvious changes in a plate.
You’ve got bacterial issues to take care of, that’s about it.
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redcrow

Registered: 03/25/22
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Re: Transfers from this plate? [Re: redcrow]
#27824229 - 06/17/22 02:11 PM (2 years, 6 months ago) |
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Update;
I made transfers from the plate in question this morning. The samples were taken from the area that's been suggested, one from denser material more inboard at about 8 o'clock and another at the same radian but nearer the edge.
Since it's been requested, I also took photomicrographs of the areas near where I took the samples from:


The top one is at approximately 50x and the bottom one was taken at 20x for a larger FOV.
I'm not mycologist enough to say these are mycelia with any degree of certainty, but they sure look like mushroom mycelia to me. That said, I'd welcome second opinions
Edited by redcrow (06/17/22 02:13 PM)
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redcrow

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Re: Transfers from this plate? [Re: redcrow]
#27824424 - 06/17/22 04:34 PM (2 years, 6 months ago) |
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Just for comparison, I poked around on Google looking for microscope mycelia images.
Found this;
Sure looks a lot like the shots I took today. Note the complete absence of fruiting bodies typical of molds
Edited by redcrow (06/17/22 04:40 PM)
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Rotnpins
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Re: Transfers from this plate? [Re: milkboy]
#27824432 - 06/17/22 04:43 PM (2 years, 6 months ago) |
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Quote:
milkboy said: I would just like to add Ive had many people tell me plates were enmeshed with mold, never found it to be true, always mycelium.
This APE plate for example, did one additional transfer and sent to 1qt of grain, produced 6 flushes almost 2 ozs dry.

Funny.. I just tossed 2 APE plates the looked Exactly like that because someone said they were mold.. makes me wonder 🤔 next time I may not be so quick to just toss plates
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Nichrome
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Re: Transfers from this plate? [Re: Rotnpins]
#27824555 - 06/17/22 05:44 PM (2 years, 6 months ago) |
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Quote:
redcrow said: Update;
I made transfers from the plate in question this morning. The samples were taken from the area that's been suggested, one from denser material more inboard at about 8 o'clock and another at the same radian but nearer the edge.
Since it's been requested, I also took photomicrographs of the areas near where I took the samples from:


The top one is at approximately 50x and the bottom one was taken at 20x for a larger FOV.
I'm not mycologist enough to say these are mycelia with any degree of certainty, but they sure look like mushroom mycelia to me. That said, I'd welcome second opinions
All fungi cells are referred to as mycelium or mycelia, including both mushrooms and molds.
Good luck with that culture. I bet it works out just fine.
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milkboy
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Re: Transfers from this plate? [Re: Rotnpins]
#27824632 - 06/17/22 06:34 PM (2 years, 6 months ago) |
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Quote:
Rotnpins said:
Quote:
milkboy said: I would just like to add Ive had many people tell me plates were enmeshed with mold, never found it to be true, always mycelium.
This APE plate for example, did one additional transfer and sent to 1qt of grain, produced 6 flushes almost 2 ozs dry.

Funny.. I just tossed 2 APE plates the looked Exactly like that because someone said they were mold.. makes me wonder 🤔 next time I may not be so quick to just toss plates 
Resulted in this (first grow actually) later flushes made much bigger mushys 
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Rotnpins
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Re: Transfers from this plate? [Re: milkboy]
#27824654 - 06/17/22 06:45 PM (2 years, 6 months ago) |
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Quote:
milkboy said:
Quote:
Rotnpins said:
Quote:
milkboy said: I would just like to add Ive had many people tell me plates were enmeshed with mold, never found it to be true, always mycelium.
This APE plate for example, did one additional transfer and sent to 1qt of grain, produced 6 flushes almost 2 ozs dry.

Funny.. I just tossed 2 APE plates the looked Exactly like that because someone said they were mold.. makes me wonder 🤔 next time I may not be so quick to just toss plates 
Resulted in this (first grow actually) later flushes made much bigger mushys 

maybe they don't look exactly the same, but close enough
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redcrow

Registered: 03/25/22
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Re: Transfers from this plate? [Re: redcrow]
#27824663 - 06/17/22 06:49 PM (2 years, 6 months ago) |
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I understand that both mushroom and mold have mycelia. But I looked up images of both and the images I found do look different. In any case, the ones I found for mushrooms looked a lot like the pix I took this AM. 
I'd love it if the samples I took from that plate give rise to clean plates, but if not, it's not a tragedy. I've got a bunch of plates in process that were 'nocked' from spore plates. Pink Buffalo, KKK, A+, and Tidal Wave. Something's gotta work out.
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Nichrome
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Re: Transfers from this plate? [Re: redcrow]
#27824779 - 06/17/22 08:04 PM (2 years, 6 months ago) |
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You have a mushroom culture for sure. Unless you transferred some of that bacteria too you should have a clean P. cubensis culture. At this point it is all just about which piece is calling to you.
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redcrow

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Re: Transfers from this plate? [Re: redcrow]
#27825038 - 06/17/22 10:01 PM (2 years, 6 months ago) |
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Using my best technique, I pulled 4 samples from 2 different sites on the plate today. Those went to fresh, clean plates. I'll see how those develop. Hopefully enough to inoculate a few jars. I'm pretty optimistic at this point. Once I've got enough clean spawn, it'll go to shoe boxes using your standard CVG media. Keeping my fingers crossed.
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SWBZA


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Re: Transfers from this plate? [Re: Nichrome]
#28688208 - 03/05/24 11:38 PM (10 months, 6 days ago) |
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Quote:
Nichrome said: What kind of mold do you see? What kind of mold do you get from cultures that look like this?
I don't see any mold.
Here is an example of a culture that is not moldy but sectoring in a similar way. The first picture is at the edge where the thick stuff meets the thin stuff. You can see that it is the same mycelium and that plenty of hyphae are meeting and joining between the two. The thin stuff is the same mycelium just expressing a slightly different growth pattern. The second photo is the thin area and the third is the plate photographed. These photos were from just now.
Nichrome, don't know whether replying to this old post will bump it to your awareness, but anyway: what magnification scope do I need to see what you show here so I can see whether there is trich on my agar plates?
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rumfor69
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Re: Transfers from this plate? [Re: SWBZA] 1
#28688210 - 03/05/24 11:43 PM (10 months, 6 days ago) |
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Dude even if you could magnifi to see trich...what would you do at that point. You got macroscopic cellular cutting and transfer tools? You gonna preform surgery that even brain surgeons cant? You think you gonna remove every cell? Not trying to sound condescending truly. I just ask what's the goal? Just start a bunch of MS plates...like 20. Then work with what you think looks good after 3-4 transfers then grow them all and find out then take a clone. Then take soores from fruits that look great and repeathe process into infinity.
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SWBZA


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Re: Transfers from this plate? [Re: rumfor69]
#28688234 - 03/06/24 12:03 AM (10 months, 6 days ago) |
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Quote:
rumfor69 said: Dude even if you could magnifi to see trich...what would you do at that point. You got macroscopic cellular cutting and transfer tools? You gonna preform surgery that even brain surgeons cant? You think you gonna remove every cell? Not trying to sound condescending truly. I just ask what's the goal? Just start a bunch of MS plates...like 20. Then work with what you think looks good after 3-4 transfers then grow them all and find out then take a clone. Then take soores from fruits that look great and repeathe process into infinity.
Fair question. I wrote off two more triched out shoeboxes yesterday. I've got a whole multi-q & a on a different thread helping me problem solve as I've now had to throw out more than a dozen boxes. I want to make sure my agar is as clean as I think it is to eliminate this as a source of the trich. I'm doing LAGM this year, let's see whether I can get fruit from that. Thanks
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rumfor69
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Re: Transfers from this plate? [Re: SWBZA]
#28688237 - 03/06/24 12:07 AM (10 months, 6 days ago) |
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I will tell you those dishes are not ready to go to grain so hopefully you transferred them a lot more but there is another type of thing that can happen bacteria in your grain spawn can be almost undetectable it's very difficult to identify clean spawn and what happens is you mix it up in a tub when you spawn to bulk and for some reason it's like it can't beat the race It is fighting bacteria it's immune system is weakened it can't fight off things that are in the air they are able to land in there Get a foothold and boom you get trich like before or during first flush.
My question to you is what ratio are you spawning at how much spawn are you using with how much CV?
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rumfor69
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Re: Transfers from this plate? [Re: rumfor69] 1
#28688240 - 03/06/24 12:09 AM (10 months, 6 days ago) |
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Wanna know something crazy...this is a bad culture

This thing had an infection that existed in the mycelium plane that didn't show up on the agar and would grow trich before fruit EVERY TIME
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rumfor69
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Re: Transfers from this plate? [Re: rumfor69] 1
#28688242 - 03/06/24 12:12 AM (10 months, 6 days ago) |
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Agar always seems like the holy Grail but it's really not it is a wonderful way to identify things quickly and easily but it doesn't mean that it's some full proof method there can still be things that can live and feed in an almost symbiotic way. The only real way to get good results is replication make a lot of dishes from your spores make like 10-15 multi spore dishes then transfer those to new dishes like 4 times and then if they look like the one I pictured transfer them to grain and try to fruit them keep track of everything label everything.
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SWBZA


Registered: 05/09/23
Posts: 304
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Re: Transfers from this plate? [Re: rumfor69]
#28688264 - 03/06/24 12:57 AM (10 months, 6 days ago) |
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Quote:
rumfor69 said: I will tell you those dishes are not ready to go to grain so hopefully you transferred them a lot more but there is another type of thing that can happen bacteria in your grain spawn can be almost undetectable it's very difficult to identify clean spawn and what happens is you mix it up in a tub when you spawn to bulk and for some reason it's like it can't beat the race It is fighting bacteria it's immune system is weakened it can't fight off things that are in the air they are able to land in there Get a foothold and boom you get trich like before or during first flush.
My question to you is what ratio are you spawning at how much spawn are you using with how much CV?
I've got transfers on water agar and tea agar to help with bacteria. I've use pure coir. Based on feedback from the previous thread, I PCd the coir for those two tubs for 2 hours to eliminate that. I changed my rye tek to Spitball's one to ensure they are not too wet. Also upped the PC for the grain for these last two tubs to 2 hours. Did boxes from 1:1 all the way to 1:6, pseudo casing, no pseudo casing. Spawn to bulk was done in a humidity chamber that I don't use at the moment. It is fed by a fan that filters air through poly, so it is a positive pressure environment with at least cleanish air. I switched off the fan for 2 hours before spawning to bulk in it, essentially giving me a monster size SAB. I didn't touch the spawn or coir, used a stainless steel instrument only, obviously ISOd. Before doing all this, I took all the furniture out of my dedicated shroom room and sprayed all surfaces, ceiling included, with a broad spectrum antifungal. Also sprayed all tables, etc., before putting back in the room, except for surfaces I will touch when I work - those I cleaned with ISO. Despite wearing a KN95 mask, got a nice asthma attack for my efforts. So, clean (not sterile, I know) room, SAB for noccing grains with agar, spawn to bulk in large SAB, no-touch, standard shower / brush teeth, ISO the crap out of everything like only a noob can, but my tubs trich out.
I've changed coir supplier and will use the new supply for my next tubs, and I've bought popcorn and milo. I have 3 jars of popcorn going. I'm off on leave for two weeks this Friday, and I'll make milo jars when I get back.
No idea where the trich comes from, but "trich causes trich", so I'm hunting down every possible vector in the whole chain.
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rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
Posts: 7,373
Loc: In the Gills
Last seen: 17 hours, 40 minutes
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Re: Transfers from this plate? [Re: SWBZA]
#28688267 - 03/06/24 01:06 AM (10 months, 6 days ago) |
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The words fan positive air and S.A.B should not even exist in the same sentence in any way. If air is moving ever no matter how clean you think it is then that has nothing to do with the still airbox cause it is not a still airbox at that point know what I mean?
My guess is you probably went to grain too early with those dishes. They should have been transferred 3..4...5 times and you'd have a beautiful agar plate to display that you took to grain. The real purpose of agar is it's a media plane that you can make multiple transfers to and create an axenic culture in hopefully. So unless you habe a pretty picture updated agar plate pic then I'd surmise you went to grain too soon
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SWBZA


Registered: 05/09/23
Posts: 304
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Re: Transfers from this plate? [Re: SWBZA]
#28688269 - 03/06/24 01:10 AM (10 months, 6 days ago) |
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Oh, I've also got two BRF pucks going nocced with Josex's poke. Hoping to run from bacteria, but no idea how that will help with mold, which I can't see - hence the scope. No worries, I'll keep at it.
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rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
Posts: 7,373
Loc: In the Gills
Last seen: 17 hours, 40 minutes
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Re: Transfers from this plate? [Re: SWBZA]
#28688273 - 03/06/24 01:16 AM (10 months, 6 days ago) |
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Mold is actually really easy to identify it's not something that's very sneaky because it's very aggressive it's very carnivorous it loves to overtake everything really fast If there was mold you would know it very quickly
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SWBZA


Registered: 05/09/23
Posts: 304
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Re: Transfers from this plate? [Re: rumfor69]
#28688274 - 03/06/24 01:17 AM (10 months, 6 days ago) |
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Quote:
rumfor69 said: The words fan positive air and S.A.B should not even exist in the same sentence in any way. If air is moving ever no matter how clean you think it is then that has nothing to do with the still airbox cause it is not a still airbox at that point know what I mean?
My guess is you probably went to grain too early with those dishes. They should have been transferred 3..4...5 times and you'd have a beautiful agar plate to display that you took to grain. The real purpose of agar is it's a media plane that you can make multiple transfers to and create an axenic culture in hopefully. So unless you habe a pretty picture updated agar plate pic then I'd surmise you went to grain too soon
Thanks. BTW, that's why I switched off the fan for hours - to get the air still. No matter. I'll keep transferring.
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rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
Posts: 7,373
Loc: In the Gills
Last seen: 17 hours, 40 minutes
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Re: Transfers from this plate? [Re: SWBZA]
#28688278 - 03/06/24 01:26 AM (10 months, 6 days ago) |
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Show us a pic of your grain spawn b4 you goto bulk next time so I can help. Compare it to the pics in my links of Clean Spawn Checklist and How it Should Look. I'm here to help ya friend, I hope you don't take my demeanor offensive. We're here to face science observations so we gotta let go of emotional assumptions sometimes.
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SWBZA


Registered: 05/09/23
Posts: 304
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Re: Transfers from this plate? [Re: rumfor69]
#28688279 - 03/06/24 01:28 AM (10 months, 6 days ago) |
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Quote:
rumfor69 said: Show us a pic of your grain spawn b4 you goto bulk next time so I can help. Compare it to the pics in my links of Clean Spawn Checklist and How it Should Look. I'm here to help ya friend, I hope you don't take my demeanor offensive. We're here to face science observations so we gotta let go of emotional assumptions sometimes.
No offence taken, I appreciate help.
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,438
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Re: Transfers from this plate? [Re: rumfor69]
#28688734 - 03/06/24 01:19 PM (10 months, 6 days ago) |
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Quote:
rumfor69 said: Dude even if you could magnifi to see trich...what would you do at that point. You got macroscopic cellular cutting and transfer tools? You gonna preform surgery that even brain surgeons cant? You think you gonna remove every cell? Not trying to sound condescending truly. I just ask what's the goal? Just start a bunch of MS plates...like 20. Then work with what you think looks good after 3-4 transfers then grow them all and find out then take a clone. Then take soores from fruits that look great and repeathe process into infinity.
You could serial dilute a blended piece of agar and put one drop onto multiple plates until you get a plate with just mycelium. This was the suggestion I received after I asked a question about micromanipulation for physically seperating the threads under a microscope.
Edit: thread here https://www.shroomery.org/forums/showflat.php/Number/28651301#28651301
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Edited by elasticaltiger (03/06/24 01:22 PM)
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rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
Posts: 7,373
Loc: In the Gills
Last seen: 17 hours, 40 minutes
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Serial dilution would probably work but I still doubt it's trich. Trich doesn't hide in a plate as far as I know it will show it's face. Also the process of cleanly preforming serial dilution in an SAB might be tough for OP
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