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LadysKnight
Hello Ladies


Registered: 10/09/15
Posts: 2,331
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Re: Transfers from this plate? [Re: Eggsalts]
#27817199 - 06/13/22 07:52 AM (2 years, 7 months ago) |
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Hopefully op will continue working with this culture and we will learn something.
-------------------- Don't follow leaders
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redcrow

Registered: 03/25/22
Posts: 384
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Re: Transfers from this plate? [Re: redcrow]
#27817296 - 06/13/22 09:19 AM (2 years, 7 months ago) |
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My take away from all this is it's a toss up whether this plate is worth further culturing. As I stated at the start, it's the result of no less than 3 attempts at cleaning it up and it started from a questionable source.
With that in mind, I think I'm going to abandon the effort. It's been worthwhile for the experience and it has improved my skills. But I've got a whole stack of ready to inoculate plates and two very good looking spore prints from a known to be reliable vendor. So I'm going to channel my work down that path and see what happens.
In my experience, persistence is a bit like gravity. It always wins.
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rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
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Re: Transfers from this plate? [Re: redcrow] 1
#27817324 - 06/13/22 09:43 AM (2 years, 7 months ago) |
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I'd still take a couple transfers from that 6-12 o'clock position just to see what happens. It'd be a shame to toss out what could become a good clean culture.
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LadysKnight
Hello Ladies


Registered: 10/09/15
Posts: 2,331
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Re: Transfers from this plate? [Re: rumfor69]
#27817369 - 06/13/22 10:15 AM (2 years, 7 months ago) |
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I learned that I'm not seeing enmeshed mold, instead I'm seeing the future and can tell when a clean culture will spontaneously develop moldy spawn.
-------------------- Don't follow leaders
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redcrow

Registered: 03/25/22
Posts: 384
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Re: Transfers from this plate? [Re: redcrow] 1
#27817371 - 06/13/22 10:20 AM (2 years, 7 months ago) |
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Oh, what the hell... Making one more transfer wouldn't exactly be a labor intensive task. I've got some inoculations to do with those new spore prints so I might as well do that at the same time. Like I said, persistence.
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milkboy
Child



Registered: 05/01/21
Posts: 2,525
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Re: Transfers from this plate? [Re: LadysKnight] 2
#27817383 - 06/13/22 10:27 AM (2 years, 7 months ago) |
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I would just like to add Ive had many people tell me plates were enmeshed with mold, never found it to be true, always mycelium.
This APE plate for example, did one additional transfer and sent to 1qt of grain, produced 6 flushes almost 2 ozs dry.
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Nichrome
Participle Phrase


Registered: 12/17/18
Posts: 7,669
Loc: Zone 5
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Re: Transfers from this plate? [Re: rumfor69] 1
#27817530 - 06/13/22 12:13 PM (2 years, 7 months ago) |
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Quote:
rumfor69 said: I'd still take a couple transfers from that 6-12 o'clock position just to see what happens. It'd be a shame to toss out what could become a good clean culture.
-------------------- discussions are a healthy alternative to arguments
There is only one electron, and it's you.
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Boozie
I like beer.



Registered: 02/18/10
Posts: 1,227
Loc: :ↄo⅃
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Re: Transfers from this plate? [Re: Nichrome]
#27818578 - 06/13/22 09:54 PM (2 years, 7 months ago) |
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Quote:
Nichrome said:
Quote:
rumfor69 said: I'd still take a couple transfers from that 6-12 o'clock position just to see what happens. It'd be a shame to toss out what could become a good clean culture.

Seconded.
Bet dollars to donuts that plate, while not totally clean by any means, will transfer out just fine. …Where did this “enmeshed mold” term come from, anyway? Mold is not going to just tag along and grow at the exact same pace as the healthy mycelium. It will overtake very quickly, halt healthy growth and cause very obvious changes in a plate.
You’ve got bacterial issues to take care of, that’s about it.
-------------------- "After silence, that which comes nearest to expressing the inexpressible is music." - Aldous Huxley
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redcrow

Registered: 03/25/22
Posts: 384
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Re: Transfers from this plate? [Re: redcrow]
#27824229 - 06/17/22 02:11 PM (2 years, 6 months ago) |
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Update;
I made transfers from the plate in question this morning. The samples were taken from the area that's been suggested, one from denser material more inboard at about 8 o'clock and another at the same radian but nearer the edge.
Since it's been requested, I also took photomicrographs of the areas near where I took the samples from:


The top one is at approximately 50x and the bottom one was taken at 20x for a larger FOV.
I'm not mycologist enough to say these are mycelia with any degree of certainty, but they sure look like mushroom mycelia to me. That said, I'd welcome second opinions
Edited by redcrow (06/17/22 02:13 PM)
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redcrow

Registered: 03/25/22
Posts: 384
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Re: Transfers from this plate? [Re: redcrow]
#27824424 - 06/17/22 04:34 PM (2 years, 6 months ago) |
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Just for comparison, I poked around on Google looking for microscope mycelia images.
Found this;
Sure looks a lot like the shots I took today. Note the complete absence of fruiting bodies typical of molds
Edited by redcrow (06/17/22 04:40 PM)
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Rotnpins
🤮 Rotten-Pins 🍄



Registered: 01/11/22
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Loc: in (front of) the hood
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Re: Transfers from this plate? [Re: milkboy]
#27824432 - 06/17/22 04:43 PM (2 years, 6 months ago) |
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Quote:
milkboy said: I would just like to add Ive had many people tell me plates were enmeshed with mold, never found it to be true, always mycelium.
This APE plate for example, did one additional transfer and sent to 1qt of grain, produced 6 flushes almost 2 ozs dry.

Funny.. I just tossed 2 APE plates the looked Exactly like that because someone said they were mold.. makes me wonder 🤔 next time I may not be so quick to just toss plates
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Nichrome
Participle Phrase


Registered: 12/17/18
Posts: 7,669
Loc: Zone 5
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Re: Transfers from this plate? [Re: Rotnpins]
#27824555 - 06/17/22 05:44 PM (2 years, 6 months ago) |
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Quote:
redcrow said: Update;
I made transfers from the plate in question this morning. The samples were taken from the area that's been suggested, one from denser material more inboard at about 8 o'clock and another at the same radian but nearer the edge.
Since it's been requested, I also took photomicrographs of the areas near where I took the samples from:


The top one is at approximately 50x and the bottom one was taken at 20x for a larger FOV.
I'm not mycologist enough to say these are mycelia with any degree of certainty, but they sure look like mushroom mycelia to me. That said, I'd welcome second opinions
All fungi cells are referred to as mycelium or mycelia, including both mushrooms and molds.
Good luck with that culture. I bet it works out just fine.
-------------------- discussions are a healthy alternative to arguments
There is only one electron, and it's you.
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milkboy
Child



Registered: 05/01/21
Posts: 2,525
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Re: Transfers from this plate? [Re: Rotnpins]
#27824632 - 06/17/22 06:34 PM (2 years, 6 months ago) |
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Quote:
Rotnpins said:
Quote:
milkboy said: I would just like to add Ive had many people tell me plates were enmeshed with mold, never found it to be true, always mycelium.
This APE plate for example, did one additional transfer and sent to 1qt of grain, produced 6 flushes almost 2 ozs dry.

Funny.. I just tossed 2 APE plates the looked Exactly like that because someone said they were mold.. makes me wonder 🤔 next time I may not be so quick to just toss plates 
Resulted in this (first grow actually) later flushes made much bigger mushys 
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Rotnpins
🤮 Rotten-Pins 🍄



Registered: 01/11/22
Posts: 4,738
Loc: in (front of) the hood
Last seen: 2 years, 26 days
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Re: Transfers from this plate? [Re: milkboy]
#27824654 - 06/17/22 06:45 PM (2 years, 6 months ago) |
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Quote:
milkboy said:
Quote:
Rotnpins said:
Quote:
milkboy said: I would just like to add Ive had many people tell me plates were enmeshed with mold, never found it to be true, always mycelium.
This APE plate for example, did one additional transfer and sent to 1qt of grain, produced 6 flushes almost 2 ozs dry.

Funny.. I just tossed 2 APE plates the looked Exactly like that because someone said they were mold.. makes me wonder 🤔 next time I may not be so quick to just toss plates 
Resulted in this (first grow actually) later flushes made much bigger mushys 

maybe they don't look exactly the same, but close enough
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redcrow

Registered: 03/25/22
Posts: 384
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Re: Transfers from this plate? [Re: redcrow]
#27824663 - 06/17/22 06:49 PM (2 years, 6 months ago) |
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I understand that both mushroom and mold have mycelia. But I looked up images of both and the images I found do look different. In any case, the ones I found for mushrooms looked a lot like the pix I took this AM. 
I'd love it if the samples I took from that plate give rise to clean plates, but if not, it's not a tragedy. I've got a bunch of plates in process that were 'nocked' from spore plates. Pink Buffalo, KKK, A+, and Tidal Wave. Something's gotta work out.
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Nichrome
Participle Phrase


Registered: 12/17/18
Posts: 7,669
Loc: Zone 5
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Re: Transfers from this plate? [Re: redcrow]
#27824779 - 06/17/22 08:04 PM (2 years, 6 months ago) |
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You have a mushroom culture for sure. Unless you transferred some of that bacteria too you should have a clean P. cubensis culture. At this point it is all just about which piece is calling to you.
-------------------- discussions are a healthy alternative to arguments
There is only one electron, and it's you.
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redcrow

Registered: 03/25/22
Posts: 384
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Re: Transfers from this plate? [Re: redcrow]
#27825038 - 06/17/22 10:01 PM (2 years, 6 months ago) |
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Using my best technique, I pulled 4 samples from 2 different sites on the plate today. Those went to fresh, clean plates. I'll see how those develop. Hopefully enough to inoculate a few jars. I'm pretty optimistic at this point. Once I've got enough clean spawn, it'll go to shoe boxes using your standard CVG media. Keeping my fingers crossed.
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SWBZA


Registered: 05/09/23
Posts: 304
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Re: Transfers from this plate? [Re: Nichrome]
#28688208 - 03/05/24 11:38 PM (10 months, 6 days ago) |
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Quote:
Nichrome said: What kind of mold do you see? What kind of mold do you get from cultures that look like this?
I don't see any mold.
Here is an example of a culture that is not moldy but sectoring in a similar way. The first picture is at the edge where the thick stuff meets the thin stuff. You can see that it is the same mycelium and that plenty of hyphae are meeting and joining between the two. The thin stuff is the same mycelium just expressing a slightly different growth pattern. The second photo is the thin area and the third is the plate photographed. These photos were from just now.
Nichrome, don't know whether replying to this old post will bump it to your awareness, but anyway: what magnification scope do I need to see what you show here so I can see whether there is trich on my agar plates?
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rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
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Re: Transfers from this plate? [Re: SWBZA] 1
#28688210 - 03/05/24 11:43 PM (10 months, 6 days ago) |
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Dude even if you could magnifi to see trich...what would you do at that point. You got macroscopic cellular cutting and transfer tools? You gonna preform surgery that even brain surgeons cant? You think you gonna remove every cell? Not trying to sound condescending truly. I just ask what's the goal? Just start a bunch of MS plates...like 20. Then work with what you think looks good after 3-4 transfers then grow them all and find out then take a clone. Then take soores from fruits that look great and repeathe process into infinity.
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SWBZA


Registered: 05/09/23
Posts: 304
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Re: Transfers from this plate? [Re: rumfor69]
#28688234 - 03/06/24 12:03 AM (10 months, 6 days ago) |
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Quote:
rumfor69 said: Dude even if you could magnifi to see trich...what would you do at that point. You got macroscopic cellular cutting and transfer tools? You gonna preform surgery that even brain surgeons cant? You think you gonna remove every cell? Not trying to sound condescending truly. I just ask what's the goal? Just start a bunch of MS plates...like 20. Then work with what you think looks good after 3-4 transfers then grow them all and find out then take a clone. Then take soores from fruits that look great and repeathe process into infinity.
Fair question. I wrote off two more triched out shoeboxes yesterday. I've got a whole multi-q & a on a different thread helping me problem solve as I've now had to throw out more than a dozen boxes. I want to make sure my agar is as clean as I think it is to eliminate this as a source of the trich. I'm doing LAGM this year, let's see whether I can get fruit from that. Thanks
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