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Invisibleredcrow

Registered: 03/25/22
Posts: 384
Transfers from this plate?
    #27816377 - 06/12/22 04:27 PM (2 years, 7 months ago)

What do you think? I don't think this plate is ready to be used as an inoculant. Where would you take samples from to try and clean this up a bit?



Thanks for your input.

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InvisibleLadysKnight
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Re: Transfers from this plate? [Re: redcrow]
    #27816432 - 06/12/22 04:57 PM (2 years, 7 months ago)

Looks mold enmeshed, I would not try to clean it.


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Invisiblegrownright
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Registered: 10/06/14
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Re: Transfers from this plate? [Re: redcrow]
    #27816434 - 06/12/22 04:58 PM (2 years, 7 months ago)

Basically anywhere around that bacterial spot. The culture looks good but its still a transfer away from being ready



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Invisiblegrownright
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Re: Transfers from this plate? [Re: LadysKnight]
    #27816438 - 06/12/22 04:59 PM (2 years, 7 months ago)

Quote:

LadysKnight said:
Looks mold enmeshed, I would not try to clean it.




You think so? I guess those wispier areas where theres a break in the myc do look a lil sus


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InvisibleLadysKnight
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Re: Transfers from this plate? [Re: grownright]
    #27816449 - 06/12/22 05:10 PM (2 years, 7 months ago)

Quote:

grownright said:
Quote:

LadysKnight said:
Looks mold enmeshed, I would not try to clean it.




You think so? I guess those wispier areas where theres a break in the myc do look a lil sus




That's where I see it most, yes.


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OfflineTheTimelessDon
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Registered: 03/19/22
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Re: Transfers from this plate? [Re: redcrow]
    #27816469 - 06/12/22 05:24 PM (2 years, 7 months ago)

That's an interesting growth pattern... Looks like a ghost came in and did transfers off of what would have normally been a perfect round plate, lol. 

I'd transfer sections between each of the splits...and honestly, I'd put the entire left side to a small jar of grain and see how it likes it :smile:  But this is just a grain of salt, food for thought, response...  Because I'm just happy to see you moving myc around and getting good growth, so I'm anxious to see you get some good looking fruit pics!

LK might be right, so when you do anything with this plate, make sure to take a piece of the fuzz growing between the sections and put it under your microscope and confirm for the classroom.  I don't know how to advise moving forward if it is...

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InvisibleNichrome
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Re: Transfers from this plate? [Re: TheTimelessDon]
    #27816562 - 06/12/22 06:16 PM (2 years, 7 months ago)

Can someone please show me what "enmeshed mold" is? I have honestly never seen it happen.

That is sectoring. That type of sectoring is indicative of a colony with internal competition going on. Like some of the hyphae are planning a mutiny or like a case of star bellied sneetches vs. regular sneetches. Possibly just a genetic defect. Long story short those patterns often lead to susceptibility to mold taking over but there is no mold in that culture.

Definitely not ready as inoculant with that little bacteria booger in there. More transfers.


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OfflineTheTimelessDon
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Re: Transfers from this plate? [Re: Nichrome]
    #27816586 - 06/12/22 06:28 PM (2 years, 7 months ago)

Quote:

Nichrome said:
Like some of the hyphae are planning a mutiny or like a case of star bellied sneetches vs. regular sneetches.




Awesome! :thehellwasthat:

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InvisibleLadysKnight
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Re: Transfers from this plate? [Re: Nichrome]
    #27816598 - 06/12/22 06:34 PM (2 years, 7 months ago)

Quote:

Nichrome said:
Can someone please show me what "enmeshed mold" is? I have honestly never seen it happen.

That is sectoring. That type of sectoring is indicative of a colony with internal competition going on. Like some of the hyphae are planning a mutiny or like a case of star bellied sneetches vs. regular sneetches. Possibly just a genetic defect. Long story short those patterns often lead to susceptibility to mold taking over but there is no mold in that culture.

Definitely not ready as inoculant with that little bacteria booger in there. More transfers.




I'll try. Immediately, the plate looks blurry and out of focus, but it's not the focus. That bacteria colony at the bottom is clear and crisp, as is the table and wire rack. The mycelium is blurred by the mold that can be seen in the missing wedges of mycelium around the edges and in the clear halo of mold surrounding the culture. The only way to prove it is to let it grow, but this is what I'm seeing.


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InvisibleNichrome
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Re: Transfers from this plate? [Re: LadysKnight]
    #27816788 - 06/12/22 09:05 PM (2 years, 7 months ago)

I have looked at those weak spots in sectoring like that extensively with a microscope as well as transferred those areas and so on, and I have yet to find "mold" in a singe one of them out of maybe hundreds that look like that. You are describing "symptoms" of an old wives tale but not really convincing me there is something there that is not. Up close under a microscope those missing areas are still just P. cubensis mycelium. It is just a growth pattern.

I have grown a lot of cultures that had mold. I grow many types of mold on purpose and enjoy cleaning up wild cultures and do so regularly. I have yet to see "enmeshed" mold in a P. Cubensis culture.

If this does indeed happen I really would love to know about it and would love to work with some of the stuff. I really would.

Getting mold from a culture like that in the end only "proves" susceptibility to mold, not that there is mold already there riding along. Trichoderma and the things called "cobweb" molds are extremely visible with a microscope. Trichoderma of any kind does not enmesh with P. cubensis mycelium.

The enmeshed mold idea is really old hat.

This is  mold germinating from some dirty Natalensis spores.


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InvisibleLadysKnight
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Re: Transfers from this plate? [Re: Nichrome]
    #27816817 - 06/12/22 09:21 PM (2 years, 7 months ago)

Your luck is better than mine because I have dealt with plenty of enmeshed mold, grown it for months , and not for fun. I can see the mold on the plate. Let it grow.


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InvisibleNichrome
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Re: Transfers from this plate? [Re: LadysKnight] * 1
    #27816822 - 06/12/22 09:24 PM (2 years, 7 months ago)

What kind of mold do you see? What kind of mold do you get from cultures that look like this?

I don't see any mold.


Here is an example of a culture that is not moldy but sectoring in a similar way. The first picture is at the edge where the thick stuff meets the thin stuff. You can see that it is the same mycelium and that plenty of hyphae are meeting and joining between the two. The thin stuff is the same mycelium just expressing a slightly different growth pattern. The second photo is the thin area and the third is the plate photographed. These photos were from just now.

Edited by Nichrome (06/12/22 09:41 PM)

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InvisibleBoozie
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Re: Transfers from this plate? [Re: Nichrome]
    #27816836 - 06/12/22 09:39 PM (2 years, 7 months ago)

Anywhere from 6-12 o’clock looks like prime transfer pickins’ to me. Stay away from the spotty runaway areas and that little “bacterial booger” Nichrome pointed out. More clean up is needed for sure, but you should be fine.


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InvisibleLadysKnight
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Re: Transfers from this plate? [Re: Nichrome]
    #27816851 - 06/12/22 09:53 PM (2 years, 7 months ago)

This is the 4oclock section, the little white puffy clouds that are disconnected from the rest of the mass. IME, this is mold.



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InvisibleNichrome
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Re: Transfers from this plate? [Re: LadysKnight]
    #27816852 - 06/12/22 09:54 PM (2 years, 7 months ago)

If you were to look up close you'd see it is the same species of mycelium.

Another example of the same type of sectoring that is not mold.


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discussions are a healthy alternative to arguments

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Edited by Nichrome (06/12/22 09:56 PM)

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InvisibleLadysKnight
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Re: Transfers from this plate? [Re: LadysKnight]
    #27816856 - 06/12/22 10:00 PM (2 years, 7 months ago)

Hmmm. Ok if that is true, and cube mycelium can look like disorganized wispy threads. And it's not mold but is disposed to mold. Then idkwtf a clean plate looks like.

Tougher call on your example, it's kinda blurry and out of focus.


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Edited by LadysKnight (06/12/22 10:06 PM)

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InvisibleNichrome
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Re: Transfers from this plate? [Re: LadysKnight]
    #27816871 - 06/12/22 10:11 PM (2 years, 7 months ago)

Susceptibility to mold gives the same result as if the mold were riding along which is failure and a moldy finish.


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Offlinecozmyc
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Re: Transfers from this plate? [Re: redcrow]
    #27816873 - 06/12/22 10:15 PM (2 years, 7 months ago)

In my few experiences with mold, it usually doesn't grow at the same rate as myc. It soon overtakes and grows faster.

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InvisibleLadysKnight
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Re: Transfers from this plate? [Re: Nichrome]
    #27816875 - 06/12/22 10:19 PM (2 years, 7 months ago)

Maybe op can take micropics?


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OfflineEggsalts
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Re: Transfers from this plate? [Re: cozmyc]
    #27817119 - 06/13/22 05:54 AM (2 years, 7 months ago)

I’ve had the opposite experience with plates that looked like this. If I have a nasty plate like this I transfer it to a low nute plate untill I see that little jetting of ropes come off the transfer, and the second I see them I just transfer it straight to a high nute plate. Seems to outrun the contaminant just fine.

The explanation of the competing mycelliums causing that weird growth helped put some shit together for me… thanks.


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