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OfflineOventoasted
I am a meat popsicle

Registered: 04/03/22
Posts: 21
Last seen: 9 months, 21 days
Possible LC contam?
    #27812295 - 06/09/22 02:22 PM (2 years, 7 months ago)




On Jun 7th I inoculated my LC with a clean agar sample that I liked. I used a LFH and sterile syringe. I flame sterilized each step and also wiped port with 70% ethanol each time. I extracted the agar mycelium by taking a small amount of LC from the jar and transferred it to agar dish. Using the syringe I scrapped up the mycelium and then sucked up the LC and mycelium back into the syringe. Sterilized and wiped down ports before injecting into the LC jar.

2 days later I see this white booger looking mass floating around the jar. Most LC phots I see are of fluffy clear/white tissue floating in the LC, not solid white clumps.

Could this be from having the LC under constant agitation or just unfortunate contamination?

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InvisibleNichrome
Participle Phrase
I'm a teapot User Gallery

Registered: 12/17/18
Posts: 7,669
Loc: Zone 5
Trusted Cultivator
Re: Possible LC contam? [Re: Oventoasted]
    #27812350 - 06/09/22 03:24 PM (2 years, 7 months ago)

That's too early to tell. Don't stir or swirl it at all for a couple days and watch if there are nice loose fibers that grow out of that blob. It's possibly just all your myc that is zippered together.

Don't touch it and show pictures again in two or three days.


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OfflineOventoasted
I am a meat popsicle

Registered: 04/03/22
Posts: 21
Last seen: 9 months, 21 days
Re: Possible LC contam? [Re: Nichrome]
    #27812373 - 06/09/22 03:45 PM (2 years, 7 months ago)

I was somewhat thinking that. I turned off the stirrer for now to see any changes.

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