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OfflineRobertJPaulson
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Registered: 06/05/22
Posts: 11
Last seen: 2 years, 7 months
"Bacterial" contamination avoidance
    #27810219 - 06/07/22 09:14 PM (2 years, 7 months ago)

Hello,
I keep getting what I call 'bacterial' contamination.  That's because its not mold, its just something else that makes grain or manure turn bad for the mycelium.
This type of contamination makes grain look wet, and if you spawn that grain to manure, it will make it stink, with a slightly sweet smell, but mostly making it smell like poo again. 
Why am I getting this? 
My 2 guesses are 1:  it was too hot in my closet, one of the bags felt warm when I checked it once. Or 2: my mycelium died (it was paneolus, which apparently dies sometimes when you 'shake the grains') and this bacteria is a result of the dying myc. 
As far as the grain, it was some rye that I cooked, dried duly, then bagged and pressure cooked for 90.  I transferred some healthy mycelium in a still air box.
Thanks in advance for any suggestions on how to avoid this in the future.

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Invisible0001001
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Registered: 02/04/21
Posts: 882
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Re: "Bacterial" contamination avoidance [Re: RobertJPaulson]
    #27810260 - 06/07/22 09:45 PM (2 years, 7 months ago)

How do you know your mycelium was healthy? There was no bacteria on the agar dish?

How long are you drying your grains before pressure cooking?

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OfflineForresterM
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Re: "Bacterial" contamination avoidance [Re: 0001001] * 2
    #27810470 - 06/08/22 05:02 AM (2 years, 7 months ago)

For bags you probably need to pressure cook a lot longer than 90 minutes, that's for jars.  So either your grain wasn't sterile when you inoculated or you got a contam in when inoculating.  I think it's 2.5 or 3 hours or more for bags, but I don't remember I don't use them for grain.


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Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
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Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.

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OfflineRobertJPaulson
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Registered: 06/05/22
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Last seen: 2 years, 7 months
Re: "Bacterial" contamination avoidance [Re: Forrester]
    #27810720 - 06/08/22 10:42 AM (2 years, 7 months ago)

That makes sense.

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OfflineRobertJPaulson
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Registered: 06/05/22
Posts: 11
Last seen: 2 years, 7 months
Re: "Bacterial" contamination avoidance [Re: 0001001]
    #27810721 - 06/08/22 10:43 AM (2 years, 7 months ago)

I dry my grains overnight.
Ah, this is a problem for me.
What does the bacteria look like on the agar dish?

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Invisible0001001
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Registered: 02/04/21
Posts: 882
Loc: Inside an opium den
Re: "Bacterial" contamination avoidance [Re: RobertJPaulson] * 1
    #27810972 - 06/08/22 03:24 PM (2 years, 7 months ago)

Quote:

RobertJPaulson said:
I dry my grains overnight.
Ah, this is a problem for me.
What does the bacteria look like on the agar dish?




Inspect your agar good and flip the dish over holding it up to the light, its fairly easy to spot. If you don't catch it early on then mycelium can grow over any bacteria and you risk transfering it to grain.


Look at one of my recent dishes. Lol.
The bacteria looks like a mushroom :rofl2:

Here is the top of the dish. Looks good, right?





Here is the bottom of the dish. Not so good.



Bacteria on agar




Yeast on agar





Yeasts generally are more larger colonies than that of bacteria

The yeast have a flattened edge and appear glossy mucoid in the center whereas the bacterial colonies do not have a flattened edge and is smooth edged mostly.

The yeast colonies look fused mostly whereas bacterial may appear more separate and distinct.

The Yeast generally give a distinct cheesy-kind of smell or bakery kind of smell.



Hamza, N. H. (2020). Bacteria and yeast on agar [Photo].


You don't want yeast, mold or bacteria on your agar dishes. Hopefully you know how to spot good mycelium? Take your germ transfer early on and then subsequent transfers from the leading edge of the mycelium.


Edited by 0001001 (06/08/22 03:34 PM)

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OfflineRobertJPaulson
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Registered: 06/05/22
Posts: 11
Last seen: 2 years, 7 months
Re: "Bacterial" contamination avoidance [Re: 0001001]
    #27811377 - 06/08/22 08:59 PM (2 years, 7 months ago)

Thanks, this helped a lot.

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