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InvisibleJosex
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
    #24256909 - 04/19/17 10:41 AM (7 years, 8 months ago)

No Ham, it's quite different. The cap in the pic really looks like it would melt in the pc. I'll try to find pics on google to show you.

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InvisibleJosex
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Josex]
    #24256925 - 04/19/17 10:48 AM (7 years, 8 months ago)



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InvisibleJosex
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Josex]
    #24256939 - 04/19/17 10:53 AM (7 years, 8 months ago)

They are always transparent btw and you can pc the shit out of them :thumbup:

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OfflinehamloafM
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Josex]
    #24256950 - 04/19/17 10:55 AM (7 years, 8 months ago)

Interesting.  Wonder if those little caps that vendors send syringes out with are autoclavable.  They look a lot like what you have posted, but what you have posted would be better, so one wouldn't have to mess with getting the needle sterilized separately, then having to get the sterilized parts together again.  Thanks for sharing!


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How I Get Stuff done. - My Reference Guide. - My Grows.

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InvisibleJosex
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
    #24256995 - 04/19/17 11:12 AM (7 years, 8 months ago)

Oh shit, and to think that I wrote the LC tek in my journal thinking that everybody has access to those types of caps :facepalm: For the few of you that read my journal and was like wtf!? You really need those caps to do the method.

On the other hand, glad we have something kewl here in Europe you folks from the Statea don't have, for a fucking change :hehehe:

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Invisiblemynakedrat
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Josex]
    #24257086 - 04/19/17 11:42 AM (7 years, 8 months ago)

All mine have caps.
Just Like that,
and I'm in the USA....
'Murka! Fuck yea!

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InvisiblebodhisattaMDiscordReddit
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Josex]
    #24257092 - 04/19/17 11:44 AM (7 years, 8 months ago)

We have both caps. The cap that goes over the needle hasn't held water in a syringe for me in the past.

The "syringe plugs" will

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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: bodhisatta]
    #24257847 - 04/19/17 05:27 PM (7 years, 8 months ago)

Yeah I guessed it wouldn't hold water at all, I just use them to prevent piercing the foil and to keep the needle sterile when you unwrap the syringe. The only thing to prevent water from boiling out is putting the syringes upright in the pc, needle caps have nothing to do with this.

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InvisibleSkipio
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
    #25351492 - 07/28/18 12:02 AM (6 years, 5 months ago)

Quote:

hamloaf said:...cool to room temp...



Room temp (~72°F) to inoculate too?

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InvisibleNumeroEno
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Skipio]
    #25351503 - 07/28/18 12:14 AM (6 years, 5 months ago)

Man I almost tried this again today but I didn’t have a LC lid made up.

Quote:

Skipio said:
Quote:

hamloaf said:...cool to room temp...



Room temp (~72°F) to inoculate too?




Yes. You always want your media to be room temperature when you inoculate it, whether it be agar, LC, or grain.


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MY ELECTRIC INOCULATION LOOP

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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
    #27809602 - 06/07/22 11:41 AM (2 years, 7 months ago)

Ham, or others,

I know this is an older thread, and it's possible there are other more established LC threads out there, but I liked the simplicity of this preparation.

I am wondering if it might be possible to update the original post with a couple of key points that I wasn't able to suss out with 100% confidence after reading the whole thread.

After inoculation of your GWLC, what is the gameplan after that?

Do we stir plate every two days?
Do we wait a set period, like 2 weeks, and then stir up?

How much is best to stir, what frequency? If plate not handy, and we are using glass shard in jar...what is sufficient?

How long can GWLC be on the shelf, shaken up and used? Is there a point at which it's dangerous to inoculate, even if you're already sure that the contents are contam free?

Is it dumb to store LC in the fridge?

Any additional info you could think to add to OP would be dope to cover the questions i can think to ask, and I'm sure there are points that I'm forgetting.

For getting the LC out of the jar the best method i could envision was a sterile syringe, draw material in, then remove, in SAB or in front of flow hood.

Thanks

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