Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Injection Grain Bag   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Substrate Bags   Myyco.com Isolated Cubensis Liquid Culture For Sale   Original Sensible Seeds Bulk Cannabis Seeds   PhytoExtractum Maeng Da Thai Kratom Leaf Powder

Jump to first unread post Pages: 1
Offlinechaoscity
Stranger
 User Gallery
Registered: 02/23/22
Posts: 14
Last seen: 2 years, 6 months
is this transfer strong enough?
    #27805697 - 06/04/22 01:01 PM (2 years, 7 months ago)

Like the title says I'm wondering if this transfer is strong enough, within 3 days it had growth maybe even less. Here are some pictures, thanks for you time.


Extras: Filter Print Post Top
OfflineHealing Oakland
Mushroom Co-op

Registered: 06/03/22
Posts: 146
Last seen: 4 days, 4 hours
Re: is this transfer strong enough? [Re: chaoscity]
    #27805737 - 06/04/22 01:51 PM (2 years, 7 months ago)

Strong enough for what? Strong enough to put to grain or to make another transfer? No to grain, yes to transferring to another plate.


--------------------
Healing Oakland
The Magic Mushroom Co-operative

Are you a cultivator in or near the Bay Area?

:pm: Healing Oakland is accepting new cultivators :pm:
We are also accepting applications for co-op assistants and customer service reps.

OAKLAND  DECRIMINALIZED

Extras: Filter Print Post Top
Offlinechaoscity
Stranger
 User Gallery
Registered: 02/23/22
Posts: 14
Last seen: 2 years, 6 months
Re: is this transfer strong enough? [Re: Healing Oakland]
    #27805747 - 06/04/22 02:02 PM (2 years, 7 months ago)

yes grain, when will I know if it is? just a more vibrant white?

Extras: Filter Print Post Top
OfflineChdeda
Stranger
Registered: 06/06/22
Posts: 1
Last seen: 2 years, 7 months
Re: is this transfer strong enough? [Re: chaoscity]
    #27807785 - 06/06/22 01:57 AM (2 years, 7 months ago)

When transferring to grain you want rhizomorphic mycelium which has well defined strands. Right now all you have is tomentose mycelium which is fuzzy and cotton like. Look up some pictures and keep transferring to agar until you have strong rhizomorphic growth.

Extras: Filter Print Post Top
OfflineDERRAYLD
Constructus
Male User Gallery

Registered: 05/13/02
Posts: 12,109
Loc: South Africa
Last seen: 7 hours, 43 minutes
Trusted Cultivator
OG Cultivator
Re: is this transfer strong enough? [Re: Chdeda]
    #27807792 - 06/06/22 02:09 AM (2 years, 7 months ago)

Quote:

Chdeda said:
When transferring to grain you want rhizomorphic mycelium which has well defined strands. Right now all you have is tomentose mycelium which is fuzzy and cotton like. Look up some pictures and keep transferring to agar until you have strong rhizomorphic growth.




That's not true, they're both just fine.
This plate is weak and has contamination riding along.

Extras: Filter Print Post Top
OfflineBobbins
Stranger
Registered: 02/02/22
Posts: 445
Last seen: 2 years, 3 months
Re: is this transfer strong enough? [Re: chaoscity]
    #27807846 - 06/06/22 04:05 AM (2 years, 7 months ago)

Is it only me that sees a very prominent brown mold throughout?


--------------------
DeALeRsHrOoMs

Extras: Filter Print Post Top
OfflineDERRAYLD
Constructus
Male User Gallery

Registered: 05/13/02
Posts: 12,109
Loc: South Africa
Last seen: 7 hours, 43 minutes
Trusted Cultivator
OG Cultivator
Re: is this transfer strong enough? [Re: Bobbins]
    #27807859 - 06/06/22 04:33 AM (2 years, 7 months ago)

No it definitely has enmeshed contamination.

Extras: Filter Print Post Top
OfflineMcCrumblton
Sir Cookie
Male


Registered: 04/12/22
Posts: 86
Loc: Ohio
Last seen: 1 year, 2 months
Re: is this transfer strong enough? [Re: DERRAYLD]
    #27807987 - 06/06/22 07:24 AM (2 years, 7 months ago)

doesn't rizo growth happen when it's lacking nutrients? not sure still new but i've been told countless times that both forms of mycelium is okay, just depends if it's clean


--------------------
'Tis is I who said "let us be friends!" or so the story goes.....

Extras: Filter Print Post Top
OfflineDERRAYLD
Constructus
Male User Gallery

Registered: 05/13/02
Posts: 12,109
Loc: South Africa
Last seen: 7 hours, 43 minutes
Trusted Cultivator
OG Cultivator
Re: is this transfer strong enough? [Re: McCrumblton]
    #27808016 - 06/06/22 07:51 AM (2 years, 7 months ago)

Not really, there are various different types of mycelium,  we just focus on  the 2 most commonly recognized.

Rusty whyte for example is super tormentose no matter what you do.

Extras: Filter Print Post Top
InvisibleNichrome
Participle Phrase
I'm a teapot User Gallery

Registered: 12/17/18
Posts: 7,669
Loc: Zone 5
Trusted Cultivator
Re: is this transfer strong enough? [Re: DERRAYLD]
    #27808050 - 06/06/22 08:29 AM (2 years, 7 months ago)

Quote:

DERRAYLD said:
Quote:

Chdeda said:
When transferring to grain you want rhizomorphic mycelium which has well defined strands. Right now all you have is tomentose mycelium which is fuzzy and cotton like. Look up some pictures and keep transferring to agar until you have strong rhizomorphic growth.




That's not true, they're both just fine.
This plate is weak and has contamination riding along.




I grow a lot of fungi that I capture from the wild. This often takes a lot of cleanup and time, and time looking through a microscope and a lot of time using logic and soul to ponder the why's and how's of what I am looking at.

There is no way to tell this for sure without a lot more information that there is "contamination" present in that culture. I have looked at thousands of cultures and have seen a good many that start out looking just like that. "Enmeshed contamination" is a very vague term that I see thrown around here a lot and it is limiting. It is very easy to just jump to "that's contaminated" when looking at anything but rhizomorphic myc patterns. Cubes are very adaptable and do not fit into the Rhizo-tomentose only box.


OP I think that culture if it is a T1 (first transfer from spore germination plate), is likely just figuring it's self out still. Often when a lot of spore is used in the beginning and depending on environment, genetics, etc mycelium can look a lot of different ways. I don't see real signs of bacteria in that plate or anything that looks like foreign mycelium from a non target species. It looks like very tomentose mycelium. That can change and that can also, although not as commonly, be just fine. There is no sectoring, walling, fighting, metabolites, slime, discoloration, variance in rapidness of growth.

Some more information would be helpful. Specifically what variety that is, how many transfers you've made, what agar recipe you used, what the ambient temps have been.

I think a lot of what people call contamination is just mycelium that is not great. Genetic issues are most often the culprit.

OP that culture if anything does not look "strong" and if you run it as is it will likely succumb to one type of invader or another. Probably trichoderma right at the time of pinning or just before.


--------------------
discussions are a healthy alternative to arguments

There is only one electron, and it's you.

Extras: Filter Print Post Top
OfflineDERRAYLD
Constructus
Male User Gallery

Registered: 05/13/02
Posts: 12,109
Loc: South Africa
Last seen: 7 hours, 43 minutes
Trusted Cultivator
OG Cultivator
Re: is this transfer strong enough? [Re: Nichrome]
    #27808100 - 06/06/22 09:03 AM (2 years, 7 months ago)

Agreed Nichrome, it's not a definite but it would deter me from using it in the current state for fear of contamination popping up but I agree it is a very weak plate.

Extras: Filter Print Post Top
InvisibleLadysKnight
Hello Ladies
 User Gallery

Registered: 10/09/15
Posts: 2,331
Re: is this transfer strong enough? [Re: DERRAYLD]
    #27808157 - 06/06/22 09:57 AM (2 years, 7 months ago)

The problems I see are more photography related. Surface not in focus and a poor color choice for the agar. You really can't tell anything from these pics.
But as usual, I suspect mold.


--------------------
Don't follow leaders

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: North Spore Injection Grain Bag   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Substrate Bags   Myyco.com Isolated Cubensis Liquid Culture For Sale   Original Sensible Seeds Bulk Cannabis Seeds   PhytoExtractum Maeng Da Thai Kratom Leaf Powder


Similar ThreadsPosterViewsRepliesLast post
* Rhizomorphic growth/Large Fruits no-tone 3,651 9 12/18/03 05:58 AM
by 95rh
* rhizomorphs wind_wraith 1,541 2 06/06/01 01:09 PM
by wind_wraith
* differences betwen strain isolation by transfers and by cloning?
( 1 2 3 4 all )
FGL 15,467 62 10/06/09 08:13 PM
by feelfunny
* respawn? myc transfer? criddopher 388 2 08/20/07 09:31 AM
by criddopher
* What mould is this? Enmeshed?
( 1 2 all )
LozMyKeys 716 26 03/22/22 10:02 AM
by LozMyKeys
* rhizomorphic isolate going back to linear / tomentose ohmatic 4,770 15 01/19/05 01:47 PM
by tripndicular
* Is this ready to transfer??? Junkaboy 669 2 03/16/05 01:18 PM
by Olgualion
* Rhizomorphic growth=ready to fruit? soochi 3,950 8 06/13/03 12:36 PM
by soochi

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
379 topic views. 20 members, 103 guests and 83 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2025 Mind Media. Some rights reserved.

Generated in 0.022 seconds spending 0.007 seconds on 12 queries.