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Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 15,116
Loc: Hole
Last seen: 11 minutes, 50 seconds
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Very slow incubation times
#27807161 - 06/05/22 03:47 PM (2 years, 7 months ago) |
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Hello all!
So I started in Uncle Bens bags initially. The silliness of that aside, I’ve moved on to quart mason jars of grain.
I’ve noticed in the time it took uncle Ben’s bags to fully colonize, colonization hasn’t even started in the quart jars. Any idea why this might be the case?
I’m trying to grow GT from spore while I wait for my agar to become viable. 75-80° temp. Moisture content looks good. The UB bags were more moist than this, for sure.
Do you think it’s possible the glass is insulating the grain enough that it is too cool inside? Should I crank the temp a bit higher?
Any advice is appreciated
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WyoMX

Registered: 07/06/15
Posts: 2,351
Loc: PNW
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Your Temps are fine and definitely not the problem so no need to crank it up. UB bags being so moist is part of why they are so frowned upon as being that wet is prime breeding grounds for bacteria so it's good that your grains are not as wet as them. How did you make your lids for the jars? Is there a hole for gas exchange? Also going spore directly to grain is more then likely the reason. It can take like 20 days for spores to germinate while sometimes you'll see germination in 4 days it all just depends on the spores doing there thing. Once you've got your agar going you won't have to worry about that though as you'll be transferring live mycelium to the grains. I'd just look at this run like practice, if you get some fruits cool but don't count your chickens before they hatch as going spore to grain often ends in failure.
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Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 15,116
Loc: Hole
Last seen: 11 minutes, 50 seconds
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Re: Very slow incubation times [Re: WyoMX]
#27807237 - 06/05/22 04:57 PM (2 years, 7 months ago) |
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Quote:
WyoMX said: Your Temps are fine and definitely not the problem so no need to crank it up. UB bags being so moist is part of why they are so frowned upon as being that wet is prime breeding grounds for bacteria so it's good that your grains are not as wet as them. How did you make your lids for the jars? Is there a hole for gas exchange? Also going spore directly to grain is more then likely the reason. It can take like 20 days for spores to germinate while sometimes you'll see germination in 4 days it all just depends on the spores doing there thing. Once you've got your agar going you won't have to worry about that though as you'll be transferring live mycelium to the grains. I'd just look at this run like practice, if you get some fruits cool but don't count your chickens before they hatch as going spore to grain often ends in failure.
I’m using these jars as something to experiment on while I get agar up. I had relative “success” with UB. 20 bags no contams and a more than adequate yield for personal use. Not a good yield by real metrics, but plenty for me.
So it’s likely just spores being spores. That’s cool. I’m using bod’s old poly fill lid tek atm. He had good success with it, so I figured it’s good on a budget.
Brand new to grains and agar, so I’m sure I’ll make even more mistakes than I did with my first run of UB. I’m really tempted to do another UB grow for fun now that I’ve realized all the mistakes that can be made with them, but I probably won’t waste my time. Although I do have another spore syringe just chillin I probably won’t need…
Anyway I’m rambling. Thanks for the input!
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