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OfflineAngelmz
Student
I'm a teapot

Registered: 06/05/22
Posts: 11
Loc: New Mexico
Last seen: 2 years, 4 months
First time here : I forgot to sterilize the jars
    #27806445 - 06/05/22 12:33 AM (2 years, 7 months ago)

My first time and I messed up. I filled my jars and forgot to sterilize before adding the mycelium. I snapped to my mistake and in an effort to correct my mistake I dug through the rice jars and pulled the agar chunks out. Hopefully they’ll still live, the rice was cold. I refilled the jars and put them to steam for an hour or two. The mycelium went from being all white to bruised in about 15 minutes. What do you think after reading that? Will the mycelium make it? Rookie move I know.

I also have 2 separate agar dishes that look like they have mold growing. I can’t tell if it’s contaminated or bruising. One plate it even looks like a tiny shroom growing on the agar. Can you take a look and let me know if these dishes are still viable or completely lost? Is it mold or bruising?



Will the mycelium survive?
You may choose only one
Mold or bruising on the agar cups?
You may choose only one


Votes accepted from 06/04/22 11:31 PM until the end of time
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OfflineSirPsycho
Purple Belt in Google-Fu
I'm a teapot User Gallery


Registered: 01/01/20
Posts: 7,340
Loc: Rent free in your head
Last seen: 14 hours, 30 minutes
Trusted Cultivator
Re: First time here : I forgot to sterilize the jars [Re: Angelmz]
    #27806570 - 06/05/22 05:23 AM (2 years, 7 months ago)

You won't be able to use either of that plates to inoculate anything now they they've been exposed to open air.


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Onlinechris77
Archaic Revivalist
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Registered: 01/17/22
Posts: 2,105
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Re: First time here : I forgot to sterilize the jars [Re: SirPsycho]
    #27806571 - 06/05/22 05:24 AM (2 years, 7 months ago)

Quote:

SirPsycho said:
You won't be able to use either of that plates to inoculate anything now they they've been exposed to open air.



:whathesaid:


--------------------
the observer is the observed
            j. krishnamurti

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OfflineAngelmz
Student
I'm a teapot

Registered: 06/05/22
Posts: 11
Loc: New Mexico
Last seen: 2 years, 4 months
Re: First time here : I forgot to sterilize the jars [Re: chris77]
    #27806874 - 06/05/22 11:20 AM (2 years, 7 months ago)

Sucks πŸ˜… I learned though. What an idiot, I’ve been slapping myself for it all day. I have some more supplies so I’ll get to retry it, just have to regrow and not mess up this next timeπŸ‘πŸ»πŸ˜‚

I had the pot with the steamer going. As I finished emptying the cups into the jars I snapped, you can’t steam mycelium πŸ™ƒ

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Invisible0001001
πŸ–•Κ•β€’Μα΄₯β€’Μ€Κ”πŸ–•
I'm a teapot

Registered: 02/04/21
Posts: 882
Loc: Inside an opium den
Re: First time here : I forgot to sterilize the jars [Re: Angelmz]
    #27806940 - 06/05/22 12:12 PM (2 years, 7 months ago)

Label everything in this hobby. Prep your grain and sterilize jars as soon as the grain is ready to be sterilized but not so soon that you dont have anything ready to add to it.


If you're from the usa, go on the postal website and you can get huge rolls of postal stickers for free. They work excellent for quickly needing to add a label to anything.

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OfflineAngelmz
Student
I'm a teapot

Registered: 06/05/22
Posts: 11
Loc: New Mexico
Last seen: 2 years, 4 months
Re: First time here : I forgot to sterilize the jars [Re: 0001001]
    #27806996 - 06/05/22 01:08 PM (2 years, 7 months ago)

πŸ™πŸ»Will do 😊 Thank you .

I’m going to watch these ruined jars carefully. I had 2 agar dishes that were perfect and spotless πŸ‘πŸ» So even through all the mistakes. I still have the jars in the garage. I’m just a few days from tossing them, but until the local trash truck comes, there’s just enough time to see what happens. Be a lot cooler if the mycelium didn’t contaminate in open air , makes me wonder how it grows in the wild?

I will label everything clearly this next batch around. I have fresh agar and spores. It’s a very interesting and exciting hobby that requires the respect it deserves to be successful. Absolutely amazing from all aspects. Next stop successful jar colonization, maybe πŸ˜‚πŸ€£

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