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Offlinedoordonot22
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Advice/Help 12 1lb rye berry grain bags no mycelium 2 months
    #27795348 - 05/27/22 04:08 PM (2 years, 7 months ago)

Hello all,

Definitely looking for advice. I bought 12 sterilized 1lb rye berry grow bags from amazon. I got 4 different strain syringes: Ecuador, B+ , Albino A+ , and Golden Teacher.

I followed all sterilization methods and inoculated 2-3 CCs after vigourous shaking and repeated sterilization into each bag (each syringe had 10ccs). I gently shook the bags after inoculation. I put regular scotch tape over the holes. So, that’s 12 bags inoculated, three of each strain.

I go on a one month trip away and they sat in a dark drawer the whole time undisturbed. The catch, they likely were too chilly most of the time, probably ranging between 50-70 degrees F. Though, likely mostly in the 50-60 F range.

I come back from the trip, see absolutely no mycelium, and realize there are a few potential reasons: it was too cold (likeliest), bad/old syringe/spores, too dry of rye berries/grain, or too little of spores in each bag.

It’s been a month since I’ve actively monitored them and still not much mycelium. Not much except one bag for Albino A+  which is showing perhaps 10 grains with partial white fuzziness/cloudiness.

Now, in the month I’ve been back, I’ve taken more effort to keep them warm. I haven’t had a thermometer, so it was all by feel. I used several methods: an IR (infrared) sauna, an IR heat pad, and, mostly recently, the oven.

The oven seems the most promising. I turn it on “warm” for 30 seconds to a minute and have put a food cooking thermometer in there. It has been around 80 degrees F the majority of the time.

I don’t know if a couple times throughout the process if I gave them too much heat for too long and killed the mycelium? Or perhaps it wasn’t enough spores in the first place? They need fresh air exchange? They need more moisture?

Yesterday I added 15ccs of boiled water reusing the same syringes and a flame for sterilization into one bag from each strain. I did this to add moisture and test if it helps.

Does anyone have any advice? Just put ‘em in a corner and forget about them for months? Throw them out? This is my second grow attempt. The first was azurescens in Denver, though I left before seeing results.

Cheers, and thanks in advance!



Edit: removed vendor name.

Edited by doordonot22 (05/27/22 10:32 PM)

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Offlinechris77
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Re: Advice/Help 12 1lb rye berry grain bags no mycelium 2 months [Re: doordonot22]
    #27795373 - 05/27/22 04:32 PM (2 years, 7 months ago)

first of all, without pictures it is far too speculative and as you said, there are many factors to the equation.

heating bags with an oven doesn't sound too good, as you said you might have damaged the few mycelium by heat.

temps between 50 and 60 are definitely too low, i am not sure if  a healthy culture would properly colonize a whole bag at 55f in reasonable time, others might clear this up.

although its most likely not what you will like to hear, i suggest you heat up your room and keep temps not much  lower than 65F (temp swings are ok), build a still air box, get spores and make your own syringes (if you dont want to start with agar this will never be a clean shot, contamination can and will often be an issue)

agar is definitely worth the effort and absolutely doable!

for the grain bags i would strongly suggest to make your own, if you dont want to invest in a pressure cooker you can have also good results with brf cakes and forget about grain bags (using brf in fruiting chambers or spawning to shoeboxes/..)

sorry to not be of more help.
post some pix and you can expect more help.


--------------------
the observer is the observed
            j. krishnamurti

Edited by chris77 (05/27/22 04:37 PM)

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Offlinehigh_desert
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Re: Advice/Help 12 1lb rye berry grain bags no mycelium 2 months [Re: doordonot22]
    #27795377 - 05/27/22 04:37 PM (2 years, 7 months ago)

Two months without germination after multispore inoculation, I don't see how those bags could still be ok. How do they look?

Although the consensus these days seems to be that cubes are ok incubating/germinating in lower temps than previously thought, I think the temp range you described is very problematic especially for an ms innoc. You can try to stabilize your temps higher now but then you're now dealing with really old sub bags that were injected with spore solution..
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Offlinehazyhorse
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Re: Advice/Help 12 1lb rye berry grain bags no mycelium 2 months [Re: high_desert]
    #27795415 - 05/27/22 05:17 PM (2 years, 7 months ago)

spore syringe directly to bag is generally discouraged too. you’re better off doing PF tek or using agar if you’re using a syringe


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InvisibleveggieM

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Re: Advice/Help 12 1lb rye berry grain bags no mycelium 2 months [Re: doordonot22]
    #27795454 - 05/27/22 05:42 PM (2 years, 7 months ago)

What is recommended to inject into sterilized grain bags is liquid inoculant, liquid culture, or semi liquid culture.

Injecting with spore solution can work and often does but with a much lower rate of success. If you do inject with a MS syringe what you do NOT want to do is then shake the bag as you did.

With the one bag that you think has mycelium let it be until the mycelium grows out to say 30% and then mix the bag gently to distribute it throughout, then go from there.

For your next attempt do the PF Tek with spore syringes. Very high success rate.

In time, as you acquire more experience and a pressure cooker, you can use the more traditional methods with agar.

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InvisibleSimulatedMatrix33
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Re: Advice/Help 12 1lb rye berry grain bags no mycelium 2 months [Re: veggie]
    #27795471 - 05/27/22 05:56 PM (2 years, 7 months ago)

After all the stuff you did, there trashed now. I can attest that the syringes weren’t the problem. More than likely you had them stored WAY too cold for any mycelium growth. You want to keep them around 75-85 range for optimal growth. Most people like to stay right below 80 degrees as the higher temps cause more bacteria growth.  You more than likely nuked any chance by putting them in the oven and never use a heating pad. Next time, don’t start a grow unless you are able to regulate temps by being readily available.  Do some Reading on the FAQs and you’ll have better luck next try.


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Everything you’ve learned and been told is a lie. You are living in a  simulated reality called the game 23. The obstacle is for you to escape this matrix. Your conscience is stored in the Black Saturn cube, you need to find a way to escape now as Judgement day is coming. I live through the conscience minds of those who seek enlightenment through way of psychedelic. I’m the messenger brought down to your realm


Edited by SimulatedMatrix33 (05/27/22 06:03 PM)

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Offlinedoordonot22
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Re: Advice/Help 12 1lb rye berry grain bags no mycelium 2 months [Re: SimulatedMatrix33]
    #27795617 - 05/27/22 07:24 PM (2 years, 7 months ago)

Thanks everyone for your responses! I’m just gonna put em in a bucket and forget about them and try again with one of your suggestions, thanks!

Low key excited about growing dmt tho

While here, anybody have any suggestions for extracting psilocybin?

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Offlinedoordonot22
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Re: Advice/Help 12 1lb rye berry grain bags no mycelium 2 months [Re: doordonot22]
    #27795625 - 05/27/22 07:28 PM (2 years, 7 months ago)




They look and smell the same as when I got em. Likely nuked them to death lol. Noob mistake looking forward to the next go.

Are those good strains though? I actually ordered Amazonian Penis Envy, but they gave me Ecuadorian instead lol. Long term plans are have outdoor grows just all around where I live out in the woods hehe. Straw, access to free horse poop, cardboard, and water. Just gonna pasteurize the straw and pooh with boiling water in buckets. Then add mycelium, wait a few months, then take em to the outdoors.

That’ll probs be after a few monotub tek/shoe boxes. I got all the materials, just need some damn mycelium lmao

Once the mycelium comes around, planning on going to coir/verm bulk sub with mono/shoeboxes. Just gotta figure out a good way to make all the temp/airflow/moisture/light automated and I’ll be gravy

Heard oven dried baking soda acts as a good way to dry out the shroomies? Called dessication? That or fan with newspapers

Edited by doordonot22 (05/27/22 07:40 PM)

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Offlinehigh_desert
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Re: Advice/Help 12 1lb rye berry grain bags no mycelium 2 months [Re: doordonot22]
    #27795648 - 05/27/22 07:41 PM (2 years, 7 months ago)

It's hard to really see but that does not look good to me. I'd be willing to bet a coffee at least some of those are bacterial.
Ive never used bags but I do mss to grain.. That moisture would not be acceptable to me in a jar for sure. Edit: you're saying they smell the same as you got them so at least there's that in your favor..

I've learned with mss-grain to be obsessive about perfect water content in your grain.. My feeling is that you can get away with a lot less wet spots and clumps using mss and it's better to be on the dry side. I don't know much about how bags are supposed to look look but that much loose funky looking water 2 months after inoculation.. I'd open them in a well ventilated area if you catch my drift.

Edited by high_desert (05/27/22 07:42 PM)

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OfflineCocaineBuffet
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Re: Advice/Help 12 1lb rye berry grain bags no mycelium 2 months [Re: high_desert]
    #27795754 - 05/27/22 08:36 PM (2 years, 7 months ago)

Take the vendor name out of your post.

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Offlinehazyhorse
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Re: Advice/Help 12 1lb rye berry grain bags no mycelium 2 months [Re: CocaineBuffet] * 1
    #27796005 - 05/28/22 02:47 AM (2 years, 7 months ago)

as far as i’ve looked into it, extracting psilocybin isn’t really worth it. it’s not like mescaline or DMT, you’re better off just doing a tea rather than trying to get crystals.

if you’re dehydrating just buy a dehydrator. the biggest killer of potency & yield is improper drying & it’s worth $40 to buy a cheap presto dehydrator from walmart. not saying you can’t dry without it but any of the heatless methods often take way too long & don’t truly dry the mushrooms

strains don’t mean shit with cubensis unless you get actual penis envy/APE or the crosses like tidal wave with notable potency. b+, cambodian, rustywhyte etc. are all gonna give you the same effects & most grow about the same. also, don’t automate anything. it sounds cool & like a good
idea when you’re starting out, but any sort of “automation” is more trouble than it’s worth. it’s easy enough to dial in a monotub to the point you don’t need to mist until after the first harvest & your efforts are better spent on learning to cultivate than they are jerry rigging some box that will end up not really working


--------------------
you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿

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Offlinedoordonot22
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Re: Advice/Help 12 1lb rye berry grain bags no mycelium 2 months [Re: hazyhorse]
    #27796107 - 05/28/22 06:33 AM (2 years, 7 months ago)

Okay good points, thank you!

Would you say there is a best strain for microdosing?

Like for productivity/motivation/focus/learning/memory/creativity?

Never really done microdosing, just heard those are some of the potential benefits 🤷♂️

That was some of the motivation behind going for azurescens the first grow; then realized they are probably better off in the wild in the PNW.

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InvisiblemushboyMDiscord
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Re: Advice/Help 12 1lb rye berry grain bags no mycelium 2 months [Re: doordonot22] * 1
    #27796119 - 05/28/22 06:55 AM (2 years, 7 months ago)

just grow cubes and do shrooms.

Quote:


Like for productivity/motivation/focus/learning/memory/creativity?





it aint weed.

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Offlinechris77
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Re: Advice/Help 12 1lb rye berry grain bags no mycelium 2 months [Re: doordonot22]
    #27796126 - 05/28/22 07:11 AM (2 years, 7 months ago)

Quote:

doordonot22 said:
Okay good points, thank you!

Would you say there is a best strain for microdosing?

Like for productivity/motivation/focus/learning/memory/creativity?

Never really done microdosing, just heard those are some of the potential benefits 🤷♂️

That was some of the motivation behind going for azurescens the first grow; then realized they are probably better off in the wild in the PNW.



just a thought..

instead of asking what the mushrooms can do for you, try to find out what you can do for the mushrooms.

have a beautiful weekend everybody


--------------------
the observer is the observed
            j. krishnamurti

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OfflineRainy21
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Re: Advice/Help 12 1lb rye berry grain bags no mycelium 2 months [Re: chris77]
    #27796528 - 05/28/22 02:51 PM (2 years, 7 months ago)

No bag shaking after innoc with spore syringe - you need the spores to come together to germinate.

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Invisible0001001
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Re: Advice/Help 12 1lb rye berry grain bags no mycelium 2 months [Re: doordonot22]
    #27796724 - 05/28/22 05:37 PM (2 years, 7 months ago)

Quote:

doordonot22 said:
Thanks everyone for your responses! I’m just gonna put em in a bucket and forget about them and try again with one of your suggestions, thanks!

Low key excited about growing dmt tho

While here, anybody have any suggestions for extracting psilocybin?





How does one grow dmt? :smbfacepalm:

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Offlinedoordonot22
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Re: Advice/Help 12 1lb rye berry grain bags no mycelium 2 months [Re: 0001001]
    #27796758 - 05/28/22 06:52 PM (2 years, 7 months ago)

I’m trying my hand at Phalaris grass for DMT. Aquatica and Brachystachys. Pros and cons to it, though figured why not test it? Definitely a bit of research before deciding.

Planted them in buckets, dirt from the backyard/firepit, put clear plastic over top, keep moist. Been a week and about 20% germinated already. Apparently 2 months or less for full life cycle. Just gonna expand it into a nice little pasture lol and get to harvesting the leaves.

Will likely try to isolate the highest potency plants/stress them for max alkaloid content. Probs gonna get a wheatgrass juicer and use one of the more basic extraction teks.

Just gotta figure out if gramine is as big as a bitch/toxic as everyone in DMT land makes it sound for Phalaris. Conflicting research so will figure out myself I guess.

Edited by doordonot22 (05/28/22 07:03 PM)

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Offlinedoordonot22
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Re: Advice/Help 12 1lb rye berry grain bags no mycelium 2 months [Re: doordonot22]
    #27804296 - 06/03/22 02:28 PM (2 years, 7 months ago)


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Invisible0001001
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Re: Advice/Help 12 1lb rye berry grain bags no mycelium 2 months [Re: doordonot22]
    #27804442 - 06/03/22 04:31 PM (2 years, 7 months ago)

You need alot of phalaris grass for extracts. I imagine if you do several smaller pulls you could get a good yield from a decent amount.

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