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OnlineSmellyhobbitM
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Beginner Agar Question
    #27804163 - 06/03/22 12:30 PM (2 years, 7 months ago)

Hello, all! I’ve been reading for the past month on agar, and I’m getting ready to pour my first plates today. In a few minutes, actually. However, I’ve run into a question I can’t find the answer to, presumably because it’s assumed to be very obvious.

Once I spore a plate and I need to isolate the healthy mycelium, how do I transfer that cut to a new agar plate? Do I need to put that specimen in an empty dish and pour more agar over it, or is the cutting placed on top of an already poured agar culture?

Thank you for your help. I am having a hard time finding the answer, so I apologize if this has been asked and answered already.

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OfflineRotnpins
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Re: Beginner Agar Question [Re: Smellyhobbit]
    #27804174 - 06/03/22 12:37 PM (2 years, 7 months ago)

You cut it and put it into another plate of agar that you've pre poured...

Pour a bunch of plates, wrap them and let them sit for 7-10days to make sure they're not contaminated... plan ahead and make sure you'll have plates ready for transfer

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Re: Beginner Agar Question [Re: Rotnpins]
    #27804177 - 06/03/22 12:39 PM (2 years, 7 months ago)

Ok thank you. Yeah I’m sure I’m just overthinking it lol. From pictures it always looks to me to be under more agar, not on top. But it makes sense since agar cuttings inoculate grain jars 🤷♂️

Thank you very much.

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Re: Beginner Agar Question [Re: Smellyhobbit]
    #27804179 - 06/03/22 12:40 PM (2 years, 7 months ago)

No problem..
Check out the agar links in my signature.. I'm pretty sure you'll find them helpful

:cheers::goodluck:

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Re: Beginner Agar Question [Re: Rotnpins]
    #27804873 - 06/03/22 08:49 PM (2 years, 7 months ago)

That first link with the colored agar actually would have perfectly answered this question. Great resources thanks again

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