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SimulatedMatrix33
Inter-dimensional Being



Registered: 04/09/22
Posts: 529
Loc: Planet Kai. Hacker of the Matr...
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Once agar plate transfer is cleaned?
#27802645 - 06/02/22 01:12 PM (2 years, 7 months ago) |
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Alright so my question is, after successfully transferring agar to the point where you have a clean plate with no contams visible. About how many jars/bags can you inoculate with that clean mycelium. I’m talking about your standard Petri-dish
-------------------- My L.A.G.M 2023 grow log
Everything you’ve learned and been told is a lie. You are living in a simulated reality called the game 23. The obstacle is for you to escape this matrix. Your conscience is stored in the Black Saturn cube, you need to find a way to escape now as Judgement day is coming. I live through the conscience minds of those who seek enlightenment through way of psychedelic. I’m the messenger brought down to your realm
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PBJ710
Strangler


Registered: 07/05/19
Posts: 3,301
Last seen: 12 hours, 53 minutes
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It only takes a tiny wedge to inoculate spawn, so depends how many transfer sized pieces you can make from clean sections of the plate. More clean inoculant = faster colonization. If you're going to do bags, I would suggest using a grain master or LC to keep colonization times down to 10-14 days.
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Ankhseramun
Wanderer



Registered: 10/11/21
Posts: 589
Loc: Cantina, Mos Eisley, Tatooine
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Some people use 1/8 or 1/6 of a plate for one half pint or 1qt jar but I use either half pint or 1/3qt jars so they're not that big and I use wedges around 1cm x 1cm (.5" x .5") in size. I also drop two or three of them, depending on how much of it I have left, in there to speed things up.
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Guerrilla
Original Asshole


Registered: 01/30/21
Posts: 3,179
Loc: United Kingdom
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Re: Once agar plate transfer is cleaned? [Re: Ankhseramun]
#27802983 - 06/02/22 05:04 PM (2 years, 7 months ago) |
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I used to do wedges around 1/6th of the plate per jar. Got bored of the colonisation times and started using half a plate.
If both your agar and technique are good you won't have a problem with half a plate. Heck, a whole plate if you really wanted.
I like to go with half a plate so I can get 2 jars out of each plate that colonise quickly. I always have a lot of plates around so it's not an issue for me. Faster is better for me whereas more spawn per plate is better for some.
-------------------- Being pissed on does not make you a real man.
...OR DOES IT?
Edited by Guerrilla (06/02/22 05:06 PM)
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Nimpo
Big Black


Registered: 05/10/12
Posts: 2,609
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Re: Once agar plate transfer is cleaned? [Re: Ankhseramun]
#27802984 - 06/02/22 05:04 PM (2 years, 7 months ago) |
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I slice a plate into 4 and each wedge goes into a different jar
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fungusul
Fungus Kingdom


Registered: 07/16/20
Posts: 1,104
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Re: Once agar plate transfer is cleaned? [Re: Nimpo]
#27803393 - 06/02/22 09:36 PM (2 years, 7 months ago) |
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I slice a plate like a chess table and put all of them into a single jar.
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san pedro guy
Captain



Registered: 10/22/17
Posts: 1,806
Last seen: 25 days, 6 hours
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Re: Once agar plate transfer is cleaned? [Re: fungusul]
#27803413 - 06/02/22 09:53 PM (2 years, 7 months ago) |
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I usually do one final tiny transfer then drop the whole plate, then shake after 4-5 days.
No point in dropping a tiny wedge, torturing yourself waiting for a jar to colonize, unless your into that kinda thing
-------------------- Noob Grow Along 2022
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0001001
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Registered: 02/04/21
Posts: 882
Loc: Inside an opium den
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Re: Once agar plate transfer is cleaned? [Re: san pedro guy]
#27803558 - 06/03/22 12:24 AM (2 years, 7 months ago) |
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I will take an entire plate and slice it into several sections like a pizza then drop that into a jar that is only maybe an 1/8th full of rye.
Once I see the the mycelium take hold and jump off the agar onto the grain I shake it up. It will colonize fast and you can then g2g transfer jars with that.
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