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Guerrilla
Bumbaclart


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Re: Official P. Subtropicales/Semperviva Thread [Re: rockyfungus]
#27799980 - 05/31/22 09:12 AM (1 year, 8 months ago) |
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Quote:
rockyfungus said:
Quote:
Doctor Mario said: They're really stingy with those things too. The cap will fold Ober on itself and some some spore will drop but even then, most of them stay in the gills. I gave up in prints right away and went directly to swabs.
Yeah, lab is closed down and had no swabs available so had to make due with foil.
With past experiments I'll get a heavy initial drop and limited time to move to foil. They curl and dehydrate super quickly on foil. Then again i'm printing at 20% RH.
Maybe droplets of water on the cap would help in this instance? And a glass or other container to retain humidity?
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rockyfungus
dirty


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Re: Official P. Subtropicales/Semperviva Thread [Re: Guerrilla]
#27799988 - 05/31/22 09:18 AM (1 year, 8 months ago) |
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No luck, doesn't seem to work as well as cubes. Anyone with really dialed in tubs notice better spore production?
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Melliferous
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Re: Official P. Subtropicales/Semperviva Thread [Re: YoshiTrainer] 1
#27800010 - 05/31/22 09:35 AM (1 year, 8 months ago) |
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Quote:
YoshiTrainer said: Thank you for straightening that out Dr. Mario. I just found this at the spore working website.
"It has been noted as slow growing and highly resistant to contamination and decay, which explains the descriptive name βsemperviva" which means ever-living. This is especially appropriate considering that this sample was originally collected over 50 years ago and is still going stong. Guzman (1978) eventually determined that P. semperviva was actually a nonpapillate variant of P. hoogshagenii and was then known as P. hoogshagenii var. convexa.
It remained under that moniker until genetic testing recently revealed the species to be conspecific with Psilocybe subtropicalis, only distantly related to P. hoogshagenii.
Psilocybe subtropicalis is the older name, so that is where it stands."
So we're back to officially cultivating subtropicalis and not hoog var convexa?
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SirPsycho
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Re: Official P. Subtropicales/Semperviva Thread [Re: Melliferous]
#27800017 - 05/31/22 09:41 AM (1 year, 8 months ago) |
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I've also printed at very low RH, took me till 3rd flush to actually get anything decent.
They were printed in a sealed container with 2 or 3 drops of water on each cap and left for about 12 hours. Still had water on them by the end.
First couple of flushes I allowed a bit of air flow and they just dried up
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Melliferous
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Re: Official P. Subtropicales/Semperviva Thread [Re: SirPsycho] 2
#27800020 - 05/31/22 09:42 AM (1 year, 8 months ago) |
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Quote:
SirPsycho said: I've also printed at very low RH, took me till 3rd flush to actually get anything decent.
They were printed in a sealed container with 2 or 3 drops of water on each cap and left for about 12 hours. Still had water on them by the end.
First couple of flushes I allowed a bit of air flow and they just dried up
The caps on mine turn up so much I get weird geometrical wisps of spores but nothing I could conclusively call a classic print.
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      "No, I don't worry. I tell you, I am a man who believed that I died 20 years ago, and I live like a man who is dead already. I have no fear, whatsoever, of anybody or anything."
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SirPsycho
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Re: Official P. Subtropicales/Semperviva Thread [Re: Melliferous] 8
#27800644 - 05/31/22 05:07 PM (1 year, 8 months ago) |
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Buried my two little pint sized cakes after 4th flush. Bag for easy clean up, layer of perlite on the bottom for drainage, cakes on top, verm to fill in around the cakes to level, soil to cover, watered with watering can. I kinda fucked it up a bit playing with he bag after so the surface is a mess but those look like a couple of nice little clusters.
As for the printing, they definitely upturn and can make some weird shapes. Some look like tri-corn hats, some are perfect little bowls though.
They do need to be left for way longer than Rocky's post though. I picked my first flushes(the two cakes in the bucket) around that time and got nothing. Someone in the daily pic thread suggested I leave them for another week when I posted second flush around the same point.
Had some success with 2nd flush but even those were picked way too early. You really gotta just wait them out until those gills are nice and black. You can see it a couple of pages back with my mini grow. Printed those on card stock, set them with hairspray and framed them I'll put up a pic in a few mins. Printed for 6ish hours with a bowl on top .
Edit:

I gotta say, even though it's a bit smudged cause the caps moved a bit... I fucking love it.
Ok I'm done jerking off
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Edited by SirPsycho (05/31/22 05:12 PM)
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SirPsycho
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Re: Official P. Subtropicales/Semperviva Thread [Re: SirPsycho] 1
#27800667 - 05/31/22 05:26 PM (1 year, 8 months ago) |
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Yo! Bridge2far, I know you lurking dude, you up dooted my last post. Check your messages, I tried to hit you up twice. I think you new message icon is bugging out.
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bridge2far


Registered: 10/09/21
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Re: Official P. Subtropicales/Semperviva Thread [Re: SirPsycho] 1
#27800689 - 05/31/22 05:40 PM (1 year, 8 months ago) |
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Quote:
SirPsycho said: Yo! Bridge2far, I know you lurking dude, you up dooted my last post. Check your messages, I tried to hit you up twice. I think you new message icon is bugging out.
Kickass. Checking messages now.
Youβre right. I didnβt get a flashing notification for your 5/26 message.
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MysticMycologist
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Re: Official P. Subtropicales/Semperviva Thread [Re: bridge2far] 1
#27800692 - 05/31/22 05:41 PM (1 year, 8 months ago) |
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I feel your pain.
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SirPsycho
Purple Belt in Google-Fu



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Re: Official P. Subtropicales/Semperviva Thread [Re: bridge2far]
#27800693 - 05/31/22 05:42 PM (1 year, 8 months ago) |
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Yeah, I've had issues with that icon last little bit and someone else just hit me back today saying they missed my message cause the icon glitched
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Edited by SirPsycho (05/31/22 05:43 PM)
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smalltalk_canceled
Babnik


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Re: Official P. Subtropicales/Semperviva Thread [Re: SirPsycho]
#27800706 - 05/31/22 05:56 PM (1 year, 8 months ago) |
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All my subtropica dying lately. Right after soaking them. All at primordia stage. pain
Meh
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Edited by smalltalk_canceled (05/31/22 05:58 PM)
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odissey
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Re: Official P. Subtropicales/Semperviva Thread [Re: SirPsycho] 3
#27801054 - 06/01/22 01:25 AM (1 year, 8 months ago) |
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Quote:
SirPsycho said:

Buried my two little pint sized cakes after 4th flush. Bag for easy clean up, layer of perlite on the bottom for drainage, cakes on top, verm to fill in around the cakes to level, soil to cover, watered with watering can. I kinda fucked it up a bit playing with he bag after so the surface is a mess but those look like a couple of nice little clusters.
As for the printing, they definitely upturn and can make some weird shapes. Some look like tri-corn hats, some are perfect little bowls though.
They do need to be left for way longer than Rocky's post though. I picked my first flushes(the two cakes in the bucket) around that time and got nothing. Someone in the daily pic thread suggested I leave them for another week when I posted second flush around the same point.
Had some success with 2nd flush but even those were picked way too early. You really gotta just wait them out until those gills are nice and black. You can see it a couple of pages back with my mini grow. Printed those on card stock, set them with hairspray and framed them I'll put up a pic in a few mins. Printed for 6ish hours with a bowl on top .
Edit:

I gotta say, even though it's a bit smudged cause the caps moved a bit... I fucking love it.
Ok I'm done jerking off
Lovely piece of art 
This is what happens when you leave them too long

Seriously though, there are 2 things that made my prints sucessful. First is leaving them really long until they have spores on the caps. So even a bit past this:

The second is putting drops of water on the caps when printing and leaving them for a long time printing. My first time printing I was really disappointed beacause at 12 hours there were no spores. I added the drop of water and waited a day and a half and then there were dark prints
Edited by odissey (06/01/22 03:37 AM)
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A.k.a
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Re: Official P. Subtropicales/Semperviva Thread [Re: odissey]
#27801190 - 06/01/22 07:14 AM (1 year, 8 months ago) |
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Idk if it was my airflow/moisture levels or just the culture, but by the time mine had spores the caps had curled back so far they were like little balls 
Always makes me nervous trying to print stuff with gills that exposed. Although the swabs came out fine so I probably shouldβve just tried printing.
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SirPsycho
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My last mexicana grow did that, hopefully it behaves better outdoors.
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Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
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mycorry
The Empirical



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Re: Official P. Subtropicales/Semperviva Thread [Re: A.k.a] 4
#27802559 - 06/02/22 11:16 AM (1 year, 8 months ago) |
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Quote:
A.k.a said: Idk if it was my airflow/moisture levels or just the culture, but by the time mine had spores the caps had curled back so far they were like little balls 
Always makes me nervous trying to print stuff with gills that exposed. Although the swabs came out fine so I probably shouldβve just tried printing.
IME The tight curling fades with each successive flush.
Here is an older post of mine where I show my own mini mono grow go from tight curled caps on the first flush to almost no curl by flush #8. By the time flush #12 matured the caps edges were saggy and hanging as if they couldn't hold up the weight. This is part of my own personal guess as to why wild mushrooms look so unique, because (dpending on the culture and it's environment)they are potentially on flush#100+, trying to suck last years broken down biological material for this years fruiting.
*I used to use an intensely nutritious substrate recipe that would give a single cake of subtropicales all the nutes it would need for 10ish months of growth. Fruit-->wait until stems turn a few shades darker to harvest--> dunk for 8 hrs-->recase--> repeat. I don't really do that anymore
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mycorry
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Re: Official P. Subtropicales/Semperviva Thread [Re: odissey]
#27802564 - 06/02/22 11:19 AM (1 year, 8 months ago) |
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Quote:
odissey said:

This is right about where I harvest. Once the stems start to turn that translucent mocha color and the caps change from orange to brownish. IME this is the optimal maturity for printing too.
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Adas
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Re: Official P. Subtropicales/Semperviva Thread [Re: mycorry]
#27802568 - 06/02/22 11:28 AM (1 year, 8 months ago) |
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Quote:
mycorry said:
Quote:
A.k.a said: Idk if it was my airflow/moisture levels or just the culture, but by the time mine had spores the caps had curled back so far they were like little balls 
Always makes me nervous trying to print stuff with gills that exposed. Although the swabs came out fine so I probably shouldβve just tried printing.
IME The tight curling fades with each successive flush.
Here is an older post of mine where I show my own mini mono grow go from tight curled caps on the first flush to almost no curl by flush #8. By the time flush #12 matured the caps edges were saggy and hanging as if they couldn't hold up the weight. This is part of my own personal guess as to why wild mushrooms look so unique, because (dpending on the culture and it's environment)they are potentially on flush#100+, trying to suck last years broken down biological material for this years fruiting.
*I used to use an intensely nutritious substrate recipe that would give a single cake of subtropicales all the nutes it would need for 10ish months of growth. Fruit-->wait until stems turn a few shades darker to harvest--> dunk for 8 hrs-->recase--> repeat. I don't really do that anymore
Cool! Do you find that they lose potency with late flushes? I've heard someone say that about Tamps.
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Hindsight
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Re: Official P. Subtropicales/Semperviva Thread [Re: Adas]
#27802591 - 06/02/22 12:00 PM (1 year, 8 months ago) |
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I have another jar of spawn ready to give semps another shot. What is the substrate consensus these days? Seems like all the stuff Mario has been doing lately has just been on straight coir.
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Nichrome
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Re: Official P. Subtropicales/Semperviva Thread [Re: Melliferous] 1
#27803027 - 06/02/22 05:27 PM (1 year, 8 months ago) |
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They don't like to drop on foil. Try parchment.
Also leave the stem attached or at least a good portion of it.
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Nichrome
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Re: Official P. Subtropicales/Semperviva Thread [Re: Hindsight] 1
#27803038 - 06/02/22 05:30 PM (1 year, 8 months ago) |
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Quote:
Hindsight said: I have another jar of spawn ready to give semps another shot. What is the substrate consensus these days? Seems like all the stuff Mario has been doing lately has just been on straight coir.
Coir. 100%.
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