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OfflineOventoasted
I am a meat popsicle

Registered: 04/03/22
Posts: 21
Last seen: 9 months, 22 days
How many agar transfers before use?
    #27801450 - 06/01/22 11:58 AM (2 years, 7 months ago)

As the title says. How many transfer of a culture is usually done before utilizing in LC or grain spawn? Im on my third agar to agar transfer and its looking pretty good with no visible contamination on all plates.

Is this a "it depends" situation?

Here is a pic of my third agar transfers. =)


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InvisibleGuerrilla
Original Asshole


Registered: 01/30/21
Posts: 3,179
Loc: United Kingdom Flag
Re: How many agar transfers before use? [Re: Oventoasted]
    #27801459 - 06/01/22 12:16 PM (2 years, 7 months ago)

Sure it depends but generally speaking T2 is usually good if it looks clean, maybe T3.


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Being pissed on does not make you a real man.

...OR DOES IT?

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OfflineKmacmo
The aborted pin
Other


Registered: 08/14/19
Posts: 1,693
Loc: Central hemisphere
Last seen: 2 months, 14 days
Re: How many agar transfers before use? [Re: Oventoasted]
    #27801460 - 06/01/22 12:17 PM (2 years, 7 months ago)

By the 3rd and 4th transfers mine usually look acceptable for some grains, but I'll transfer upto 7 or 8 times if needed looking for best candidates


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OfflineOventoasted
I am a meat popsicle

Registered: 04/03/22
Posts: 21
Last seen: 9 months, 22 days
Re: How many agar transfers before use? [Re: Oventoasted]
    #27801466 - 06/01/22 12:27 PM (2 years, 7 months ago)

Awesome, thanks for the replies! I'll keep up the transfers any maybe send one to spawn in some grain. Everything is clean but I'm not seeing a lot of difference between them.

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Onlinenektar61S
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Registered: 07/04/20
Posts: 3,686
Loc: Hamsterdam
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Re: How many agar transfers before use? [Re: Oventoasted]
    #27801563 - 06/01/22 02:36 PM (2 years, 7 months ago)

Quote:

Oventoasted said:
Awesome, thanks for the replies! I'll keep up the transfers any maybe send one to spawn in some grain. Everything is clean but I'm not seeing a lot of difference between them.





Can be as low as one or two. It depends.

Post some clear photos.

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Invisibleoutlier52
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Registered: 02/17/22
Posts: 235
Loc: Zeta Reticuli
Re: How many agar transfers before use? [Re: nektar61]
    #27801732 - 06/01/22 05:07 PM (2 years, 7 months ago)

Quote:

nektar61 said:

Can be as low as one or two. It depends.

Post some clear photos.




This came from a neglected cup I germinated with a swab. I had better transfers from the other cups, and this cup was a fluffy mess. Right when I was going to toss the cup, I noticed two strong ropes growing up the edge; the rest of the cup was an amorphous blob.

Having shaky hands, I went in with the scalpel hoping to get both strands of rhizomorphic mycelium, thinking I’d totally screw it up if I tried too surgical of an extraction and went in for a single strand. But what happened was that I effed up my plan and by blind dumb luck perfectly removed a single strand, about half a cm long and quickly relocated it to its new digs.

It’s only been four days, but I have no doubt that barring contam this is going to grain. So one never knows how many transfers it could take, you could just get lucky.
I’ve done four or five transfers that didn’t look as good as this one.


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OfflineOventoasted
I am a meat popsicle

Registered: 04/03/22
Posts: 21
Last seen: 9 months, 22 days
Re: How many agar transfers before use? [Re: outlier52]
    #27801763 - 06/01/22 05:43 PM (2 years, 7 months ago)

Quote:

outlier52 said:
Quote:

nektar61 said:

Can be as low as one or two. It depends.

Post some clear photos.




This came from a neglected cup I germinated with a swab. I had better transfers from the other cups, and this cup was a fluffy mess. Right when I was going to toss the cup, I noticed two strong ropes growing up the edge; the rest of the cup was an amorphous blob.

Having shaky hands, I went in with the scalpel hoping to get both strands of rhizomorphic mycelium, thinking I’d totally screw it up if I tried too surgical of an extraction and went in for a single strand. But what happened was that I effed up my plan and by blind dumb luck perfectly removed a single strand, about half a cm long and quickly relocated it to its new digs.

It’s only been four days, but I have no doubt that barring contam this is going to grain. So one never knows how many transfers it could take, you could just get lucky.
I’ve done four or five transfers that didn’t look as good as this one.






oo man! im jelly! All mine are just fuzzy blobs. waiting for some rhizomorphic strands in any of my agars.

Congrats! :dancer:

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InvisibleMushroomguy420
Mushroom Aviator
I'm a teapot User Gallery


Registered: 12/26/21
Posts: 147
Loc: Hiding in your spores
Re: How many agar transfers before use? [Re: Oventoasted] * 1
    #27801781 - 06/01/22 05:58 PM (2 years, 7 months ago)

I'd say 1st to 3rd transfers. From what I'm told the more transfer you do then you are isolating out possibly good phenotypes.

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OfflineOventoasted
I am a meat popsicle

Registered: 04/03/22
Posts: 21
Last seen: 9 months, 22 days
Re: How many agar transfers before use? [Re: Mushroomguy420] * 1
    #27801949 - 06/01/22 08:30 PM (2 years, 7 months ago)

Yeah, that's good to keep in mind! Working with agar is just a lot of fun. Almost as much as growing the mushrooms themselves!

Flow hood was a game changer and now I just want to keep plating different mycelium. :biggrin:

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Onlinenektar61S
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Registered: 07/04/20
Posts: 3,686
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Re: How many agar transfers before use? [Re: outlier52]
    #27802254 - 06/02/22 04:41 AM (2 years, 7 months ago)

Quote:

outlier52 said:
Quote:

nektar61 said:

Can be as low as one or two. It depends.

Post some clear photos.




This came from a neglected cup I germinated with a swab. I had better transfers from the other cups, and this cup was a fluffy mess. Right when I was going to toss the cup, I noticed two strong ropes growing up the edge; the rest of the cup was an amorphous blob.

Having shaky hands, I went in with the scalpel hoping to get both strands of rhizomorphic mycelium, thinking I’d totally screw it up if I tried too surgical of an extraction and went in for a single strand. But what happened was that I effed up my plan and by blind dumb luck perfectly removed a single strand, about half a cm long and quickly relocated it to its new digs.

It’s only been four days, but I have no doubt that barring contam this is going to grain. So one never knows how many transfers it could take, you could just get lucky.
I’ve done four or five transfers that didn’t look as good as this one.






That looks good. I don't see any contam around it, but can't really see what's at the top of the plate, might be contam.

Read my post on taking clear pix. I think your camera is focusing on the top of the plate.

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Onlinenektar61S
Heterotroph
Female Unread Journal User Gallery


Registered: 07/04/20
Posts: 3,686
Loc: Hamsterdam
Last seen: 48 seconds
Re: How many agar transfers before use? [Re: outlier52]
    #27802255 - 06/02/22 04:43 AM (2 years, 7 months ago)

Quote:

outlier52 said:
Quote:

nektar61 said:

Can be as low as one or two. It depends.

Post some clear photos.




This came from a neglected cup I germinated with a swab. I had better transfers from the other cups, and this cup was a fluffy mess. Right when I was going to toss the cup, I noticed two strong ropes growing up the edge; the rest of the cup was an amorphous blob.

Having shaky hands, I went in with the scalpel hoping to get both strands of rhizomorphic mycelium, thinking I’d totally screw it up if I tried too surgical of an extraction and went in for a single strand. But what happened was that I effed up my plan and by blind dumb luck perfectly removed a single strand, about half a cm long and quickly relocated it to its new digs.

It’s only been four days, but I have no doubt that barring contam this is going to grain. So one never knows how many transfers it could take, you could just get lucky.
I’ve done four or five transfers that didn’t look as good as this one.






That looks good. I don't see any contam around it, but can't really see what's at the top of the plate, might be contam.

Read my post on taking clear pix. I think your camera is focusing on the top of the plate.
----
Quote:

Oventoasted said:
oo man! im jelly! All mine are just fuzzy blobs. waiting for some rhizomorphic strands in any of my agars.





Mine are almost all rhizo. Part of it is the agar mix. Read the last link in my sig line, my agar mix is in that post. Was a tweak on what D3 does, and his are all rhizo.

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