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Myyco.com Shop: Isolated Cubensis Liquid Culture For Sale

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OfflineUhhhPholiota
Life long learner
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Registered: 06/25/19
Posts: 33
Last seen: 2 years, 6 months
Sanitation of butane torch?
    #27800784 - 05/31/22 07:31 PM (2 years, 7 months ago)

So I decided to use a torch over an alcohol lamp due to space restrictions and safety for the most part. However, I realized my torch will be outside my SAB and I will be going in and out to sterilize my scalpel and inoculation loop. What's the proper sterile technique? Do I risk contamination after touching my torch or am I overthinking this? Perhaps wipe down the torch with ISO and let it dry before I began.

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OfflineWeavieWonder
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Registered: 08/18/16
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Re: Sanitation of butane torch? [Re: UhhhPholiota]
    #27800793 - 05/31/22 07:43 PM (2 years, 7 months ago)

Overthinking it, bud. Nothing needs to be sterile inside your SAB except scalpel blades or the tip of any tool that will be in contact with your culture medium. As long as the air inside the box is still and practice good sterile techniques, all will be good. Maybe wipe your gloves down with ISO every once in while. Torch should definitely be outside the box. The flame will create air currents. And ignite ISO fumes. POOF! There goes your arm hair or worse.

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OfflinePBJ710
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Registered: 07/05/19
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Re: Sanitation of butane torch? [Re: WeavieWonder]
    #27800803 - 05/31/22 07:59 PM (2 years, 7 months ago)

I just wipe my torch with ISO before a session and then occasionally wipe my gloves.

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InvisibleElf_on_a_Log
Elf

Registered: 07/13/14
Posts: 610
Loc: On a log
Re: Sanitation of butane torch? [Re: PBJ710]
    #27800842 - 05/31/22 08:54 PM (2 years, 7 months ago)

:whathesaid:
I just wipe the torch to get off any dust particles that might ride on my gloves and float off in the SAB.


--------------------
"I was strolling through the woods one day when I came upon an elf sitting on a log.  He offered me a strange-looking mushroom to eat.  As soon as I ate it, I realized that I was the elf on the log and that my human life had been but a dream."
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OfflineBlueAndOrange
Psychedelic success story…
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Registered: 01/11/22
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Re: Sanitation of butane torch? [Re: Elf_on_a_Log]
    #27800938 - 05/31/22 10:33 PM (2 years, 7 months ago)

I am probably a little neurotic about sterile technique, but I wipe my gloves with alcohol frequently while working. I retired my torch for a foot operated induction sterilizer. Best upgrade since going from a SAB to a FFU. So much easier.

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Myyco.com Shop: Isolated Cubensis Liquid Culture For Sale


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