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Szaszatanek
Stranger
Registered: 05/28/22
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Easy growing PF tek
#27796964 - 05/28/22 09:44 PM (2 years, 7 months ago) |
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Hello
I usually used PFtek and spore syringes, and I had 100% no contamination. I just made a step forward and create SAB, starting with agar and liquid culture and problems has come.
All new PFteks are contaminated mainly with trichoderma..
I've got few questions: 1.Did I was lucky with using spore syringes before? I mean, no SAB, just DIY PFteks and spore syringes.(I tried to be maximum sterile all the time).
2. What is the best method to keep shroom material? LC, agar?
3. Is it possible to inoculate PF tek directly from agar?
Thanks in advance
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ModularMind
M.P.F.



Registered: 02/09/10
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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
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Quote:
Szaszatanek said: Hello
I usually used PFtek and spore syringes, and I had 100% no contamination. I just made a step forward and create SAB, starting with agar and liquid culture and problems has come.
All new PFteks are contaminated mainly with trichoderma..
I've got few questions:
1. Did I was lucky with using spore syringes before? I mean, no SAB, just DIY PFteks and spore syringes.(I tried to be maximum sterile all the time).
2. What is the best method to keep shroom material? LC, agar?
3. Is it possible to inoculate PF tek directly from agar?
Thanks in advance
1) Probably lucky and good technique.
2) Probably Agar plates/slants.
3) Kinda. You have to make a LI syringe first from a section of colonized agar. PF Tek requires syringes, but the syringes can be filled with clean LC or LI instead of spores.
-------------------- "The beginnings of unlimited energy will likely emerge around 2024 and will become a worldwide manifestation by 2040. Once unlimited energy is established, the possibilities will be endless - your progressive movement will accelerate to astronomical heights." - Pleiadian Prophecy 2020 The New Golden Age by James Carwin
PROJECT BLUE BOOK ANALYSIS (PDF Download) - 312 pages! | Psychedelics & UFOs | Ready to Contact UFOs? | The Source on Mushrooms Canned Computer Duster SAB Tek π¨βπ¬ | Trippy Gematrix | Dj TeknoLogical | Fentanyl Test Kits | R.I.P. Big Worm || The Start of the Ascension Process was 2020. πππ
  Oregon Eclipse Festival 2017 :: Aug 19th - 21st :: Pure Paradise   Very Effective LSA Extraction Tek | π§ Advanced Cold Water LSA Extraction Method π§ |  Mescajuana - Mescaline with Marijuana | DMT Dab Bongs | UFO Technology! Shpongle
     
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high_desert
Pipe and Paper Afternoon



Registered: 10/25/16
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If you're using agar then you might as well just go with agar to grain. If you really want to stick with PF then make your own prints and syringes in the sab and get that procedure down. My limited experience with LC wasn't too great and I think it's probably best to get more experienced before messing with it. If you're intent on using LC then youre going to have to go through your procedures step by step and identify possible issues.. Could be a lot of things.
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PF Tek
Quick Coir/Verm Substrate
PF Cakes as Spawn
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Szaszatanek
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Registered: 05/28/22
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Quote:
LogicaL Chaos said:
1) Probably lucky and good technique.
2) Probably Agar plates/slants.
3) Kinda. You have to make a LI syringe first from a section of colonized agar. PF Tek requires syringes, but the syringes can be filled with clean LC or LI instead of spores.
Thanks.
I made my first agar from sterile spore swabs. I'm noob so im not sure that is clean, looks healthy but... I transfered it to LC.
Next questions: 1. Is it possible to get clean agar culture from swab without transfers? 2. If yes and i transfer mycelium from agar to LC is this LC will be clean as well?
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Szaszatanek
Stranger
Registered: 05/28/22
Posts: 94
Last seen: 1 year, 3 months
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Quote:
high_desert said: If you're using agar then you might as well just go with agar to grain. If you really want to stick with PF then make your own prints and syringes in the sab and get that procedure down. My limited experience with LC wasn't too great and I think it's probably best to get more experienced before messing with it. If you're intent on using LC then youre going to have to go through your procedures step by step and identify possible issues.. Could be a lot of things.
I want to stay with rice cake. I've got small 10-11PSI pressure cooker. What do you mean? Just make spores syringes in sterile water?
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high_desert
Pipe and Paper Afternoon



Registered: 10/25/16
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TheTimelessDon
The Don


Registered: 03/19/22
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Quote:
Szaszatanek said: 1.Did I was lucky with using spore syringes before? I mean, no SAB, just DIY PFteks and spore syringes.(I tried to be maximum sterile all the time). 2. What is the best method to keep shroom material? LC, agar? 3. Is it possible to inoculate PF tek directly from agar?
3. Absolutely! Cook up those jars like you would normally do. Swap the top layer of verm for a filtered lid (1/4" hole and some polyfill) (Or no filter is probably fine for smaller jars). Instead of needle/syringe, open the lid and drop in agar wedges.
Whole grains are great, but most mycelium still LOVES brf. I would suggest that method to anyone ready to jump into agar, but not quite ready to jump into PCing grains. These pf tek jars can also be broken up into bulk substrate after too, so it really opens up your options.
You will end up moving to other grains eventually I'm sure... but it is an option for anyone doing pf tek and considering agar work - agar doesn't require bulk or anything special. My recommendation is to start agar asap, drop clean myc in pf tek jars, and fruit however you are used to fruiting successfully. The only thing that changes is your inoculation method and success rate!

Oh! And a sliiiigght change in obsessions, because that's when it really gets addicting...
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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
Posts: 71,181
Loc: The Inexpressible...
Last seen: 10 minutes
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Quote:
Szaszatanek said:
Quote:
LogicaL Chaos said:
1) Probably lucky and good technique.
2) Probably Agar plates/slants.
3) Kinda. You have to make a LI syringe first from a section of colonized agar. PF Tek requires syringes, but the syringes can be filled with clean LC or LI instead of spores.
Thanks.
I made my first agar from sterile spore swabs. I'm noob so im not sure that is clean, looks healthy but... I transfered it to LC.
Next questions: 1. Is it possible to get clean agar culture from swab without transfers? 2. If yes and i transfer mycelium from agar to LC is this LC will be clean as well?
1. If you transfer the swab directly to another agar plate, then yes. 2. It should be if you select a clean portion of your colonized plate.
-------------------- "The beginnings of unlimited energy will likely emerge around 2024 and will become a worldwide manifestation by 2040. Once unlimited energy is established, the possibilities will be endless - your progressive movement will accelerate to astronomical heights." - Pleiadian Prophecy 2020 The New Golden Age by James Carwin
PROJECT BLUE BOOK ANALYSIS (PDF Download) - 312 pages! | Psychedelics & UFOs | Ready to Contact UFOs? | The Source on Mushrooms Canned Computer Duster SAB Tek π¨βπ¬ | Trippy Gematrix | Dj TeknoLogical | Fentanyl Test Kits | R.I.P. Big Worm || The Start of the Ascension Process was 2020. πππ
  Oregon Eclipse Festival 2017 :: Aug 19th - 21st :: Pure Paradise   Very Effective LSA Extraction Tek | π§ Advanced Cold Water LSA Extraction Method π§ |  Mescajuana - Mescaline with Marijuana | DMT Dab Bongs | UFO Technology! Shpongle
     
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Szaszatanek
Stranger
Registered: 05/28/22
Posts: 94
Last seen: 1 year, 3 months
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Quote:
high_desert said: Yeah that's the idea.. There's some print and syringe making teks around here.
https://www.shroomery.org/forums/printthread.php/Board/2/main/15201763/type/post
I already made it and half of it i just put into pftek and I made LC from the rest of it. Then i inoculated 2 cakes by spores and two by LC All cakes are contaminated but LC looks perfectly good, very clean liquid and fluffy myc. I read about spore syringes and LC that these is kind of lottery, both can be contaminated, is it right?
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Szaszatanek
Stranger
Registered: 05/28/22
Posts: 94
Last seen: 1 year, 3 months
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Quote:
TheTimelessDon said:
3. Absolutely! Cook up those jars like you would normally do. Swap the top layer of verm for a filtered lid (1/4" hole and some polyfill) (Or no filter is probably fine for smaller jars). Instead of needle/syringe, open the lid and drop in agar wedges.
Whole grains are great, but most mycelium still LOVES brf. I would suggest that method to anyone ready to jump into agar, but not quite ready to jump into PCing grains. These pf tek jars can also be broken up into bulk substrate after too, so it really opens up your options.
You will end up moving to other grains eventually I'm sure... but it is an option for anyone doing pf tek and considering agar work - agar doesn't require bulk or anything special. My recommendation is to start agar asap, drop clean myc in pf tek jars, and fruit however you are used to fruiting successfully. The only thing that changes is your inoculation method and success rate!

Oh! And a sliiiigght change in obsessions, because that's when it really gets addicting...
Is it a good idea to remove top vermiculite layer? I use maximum 800ml containers, now i just try with 380ml jars. I always try do things without opening anything, that's why i decided to make LC instead agar, but i read this: https://www-shroomery-org.translate.goog/forums/showflat.php/Number/18081573/fpart/4/vc/1?_x_tr_sl=en&_x_tr_tl=pl&_x_tr_hl=pl&_x_tr_pto=wapp and I made my agar pots ( no pouring method). First problem is i can't see myc properly because SAB isn't full transparent, and secondly there is some water inside. Any advice with this?
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cozmyc
gentle modern ape


Registered: 06/20/21
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I also obtained a SAB that is slightly opaque. In the top of the SAB (which would be the bottom of the container) I just cut a rectangle out for a viewing hole and covered it with some clearer plastic I had. I plan to buy a small sheet of plexi glass to replace the plastic with. Unless I find a big enough container that is also clear.
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